Crystal structure of a shark single-domain antibody V region in complex with lysozyme

Science

Volumn 305, Issue 5691, 2004, Pages 1770-1773

  Stanfield, Robyn L ^a   Dooley, Helen ^b,c   Flajnik, Martin F ^b,c   Wilson, Ian A ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c National Aquarium in Baltimore   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; ANTIGENS; BINDING ENERGY; CRYSTAL STRUCTURE;

ANTIGEN RECEPTORS; HOMODIMERS; IMMUNE RESPONSE; NANOMOLAR AFFINITY;

IMMUNOLOGY;

EGG WHITE; IMMUNOGLOBULIN HEAVY CHAIN; LYMPHOCYTE ANTIGEN RECEPTOR; LYSOZYME; DRUG DERIVATIVE; HEN EGG LYSOZYME; IMMUNOGLOBULIN; MEGLUMINE; NURSE SHARK ANTIGEN RECEPTOR; TETRAHYDROPAPAVEROLINE; UTEN;

IMMUNE SYSTEM; SHARK;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; COMPARATIVE STUDY; CRYSTAL STRUCTURE; FISH; IMMUNE RESPONSE; IMMUNE SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SHARK; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; COMPLEMENTARITY DETERMINING REGION; DIMERIZATION; DRUG COMBINATION; GENETICS; IMMUNOGLOBULIN GENE; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; METABOLISM; MOLECULAR EVOLUTION; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

CHONDRICHTHYES; VERTEBRATA;

ANIMALS; COMPLEMENTARITY DETERMINING REGIONS; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; DRUG COMBINATIONS; EVOLUTION, MOLECULAR; GENES, IMMUNOGLOBULIN; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOGLOBULINS; MEGLUMINE; MODELS, MOLECULAR; MURAMIDASE; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, ANTIGEN; SHARKS; TETRAHYDROPAPAVEROLINE;

EID: 4544250973     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1101148     Document Type: Article

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43. We thank P. Horton for technical assistance. I. Holton, G. Meigs, the staff of Advanced Light Source Beamline 8.3.1 for support during data collection, and The National Aquarium in Baltimore for meticulous care of the sharks. Supported by NIH grants GM38273 (R.L.S. and I.A.W.) and RR06603 (H.D. and M.F.F.). This is manuscript no. 16602-MB from The Scripps Research Institute. The coordinates and structure factors have been deposited in the Protein Data Bank (PDB) with accession codes 1SQ2 (crystal form 1) and 1T6V (crystal form 2).