Structural features and healthy properties of polysaccharides occurring in mushrooms

Agriculture (Switzerland)

Volumn 2, Issue 4, 2012, Pages 452-471

  Villares, Ana ^a   Mateo Vivaracho, Laura ^a   Guillamón, Eva ^a  

^a INIA   (Spain)

Author keywords

Biological response modifier; Carbohydrates; Fungus; Mushroom; Polysaccharides

Indexed keywords

EID: 84888222098     PISSN: None     EISSN: 20770472     Source Type: Journal    
DOI: 10.3390/agriculture2040452     Document Type: Article

References (101)

1. Chiron, N.; Michelot, D. Odeurs des champignons: Chimie et rôle dans les interactions biotiques—Une revue. Cryptogam. Mycol. 2005, 26, 299–364.
2. Maga, J.A. Mushroom flavor. J. Agric. Food Chem. 1981, 29, 1–4.
3. Tsai, S.Y.; Tsai, H.L.; Mau, J.L. Non-volatile taste components of Agaricus blazei, Agrocybe cylindracea and Boletus edulis. Food Chem. 2008, 107, 977–983.
4. Beluhan, S.; Ranogajec, A. Chemical composition and non-volatile components of croatian wild edible mushrooms. Food Chem. 2011, 124, 1076–1082.
5. Manzi, P.; Aguzzi, A.; Pizzoferrato, L. Nutritional value of mushrooms widely consumed in Italy. Food Chem. 2001, 73, 321–325.
6. Manzi, P.; Marconi, S.; Aguzzi, A.; Pizzoferrato, L. Commercial mushrooms: Nutritional quality and effect of cooking. Food Chem. 2004, 84, 201–206.
7. Chang, S.T.; Wasser, S.P. The role of culinary-medicinal mushrooms on human welfare with a pyramid model for human health. Int. J. Med. Mushrooms 2012, 14, 95–134.
8. Bohn, J.A.; BeMiller, J.N. (1→3)-β-D-glucans as biological response modifiers: A review of structure-functional activity relationships. Carbohydr. Polym. 1995, 28, 3–14.
9. Da Silva, M.D.C.; Fukuda, E.K.; Vasconcelos, A.F.D.; Dekker, R.F.H.; Matias, A.C.; Monteiro, N.K.; Cardoso, M.S.; Barbosa, A.M.; Silveira, J.L.M.; Sassaki, G.L., et al. Structural characterization of the cell wall D-glucans isolated from the mycelium of Botryosphaeria rhodina MAMB-05. Carbohydr. Res. 2008, 343, 793–798.
10. Muller, A.; Ensley, H.; Pretus, H.; McNamee, R.; Jones, E.; McLaughlin, E.; Chandley, W.; Browder, W.; Lowman, D.; Williams, D. The application of various protic acids in the extraction of (1→3)-β-D-glucan from Saccharomyces cerevisiae. Carbohydr. Res. 1997, 299, 203–208.
11. Lo, T.C.T.; Tsao, H.H.; Wang, A.Y.; Chang, C.A. Pressurized water extraction of polysaccharides as secondary metabolites from Lentinula edodes. J. Agric. Food Chem. 2007, 55, 4196–4201.
12. Santoyo, S.; Plaza, M.; Jaime, L.; Ibanez, E.; Reglero, G.; Senorans, F.J. Pressurized liquid extraction as an alternative process to obtain antiviral agents from the edible microalga Chlorella vulgaris. J. Agric. Food Chem. 2010, 58, 8522–8527.
13. Pereira, C.G.; Meireles, M.A.A. Supercritical fluid extraction of bioactive compounds: Fundamentals, applications and economic perspectives. Food Bioprocess Technol. 2010, 3, 340–372.
14. Fu, Y.-J.; Liu, W.; Zu, Y.-G.; Shi, X.-G.; Liu, Z.-G.; Schwarz, G.; Efferth, T. Breaking the spores of the fungus Ganoderma lucidum by supercritical CO2. Food Chem. 2009, 112, 71–76.
15. Riera, E.; Blanco, A.; Garcia, J.; Benedito, J.; Mulet, A.; Gallego-Juarez, J.A.; Blasco, M. High-power ultrasonic system for the enhancement of mass transfer in supercritical CO2 extraction processes. Ultrasonics 2010, 50, 306–309.
16. Ma, J.; Qiao, Z.; Xiang, X. Optimisation of extraction procedure for black fungus polysaccharides and effect of the polysaccharides on blood lipid and myocardium antioxidant enzymes activities. Carbohydr. Polym. 2011, 84, 1061–1068.
17. Tian, Y.; Zeng, H.; Xu, Z.; Zheng, B.; Lin, Y.; Gan, C.; Lo, Y.M. Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus). Carbohydr. Polym. 2012, 88, 522–529.
18. Zhang, Z.; Lv, G.; Pan, H.; Shi, L.; Fan, L. Optimization of the microwave-assisted extraction process for polysaccharides in himematsutake (Agaricus blazei Murrill) and evaluation of their antioxidant activities. Food Sci. Technol. Res. 2011, 17, 461–470.
19. Song, J.-F.; Li, D.-J.; Liu, C.-Q. Response surface analysis of microwave-assisted extraction of polysaccharides from cultured Cordyceps militaris. J. Chem. Technol. Biotechnol. 2009, 84, 1669–1673.
20. Ru, Q.M.; Zhang, L.R.; Chen, J.D.; Pei, Z.M.; Zheng, H.L. Microwave-assisted extraction and identification of polysaccharide from Lycoris aurea. Chem. Nat. Compd. 2009, 45, 474–477.
21. Dong, J.-Z.; Wang, Z.-C.; Wang, Y. Rapid extraction of polysaccharides from fruits of Lycium barbarum L. J. Food Biochem. 2011, 35, 1047–1057.
22. Huang, S.-Q.; Ning, Z.-X. Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity. Int. J. Biol. Macromol. 2010, 47, 336–341.
23. Chen, Y.; Gu, X.; Huang, S.-Q.; Li, J.; Wang, X.; Tang, J. Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities. Int. J. Biol. Macromol. 2010, 46, 429–435.
24. Palacios, I.; Lozano, M.; Moro, C.; D’Arrigo, M.; Rostagno, M.A.; Martínez, J.A.; García-Lafuente, A.; Guillamón, E.; Villares, A. Antioxidant properties of phenolic compounds occurring in edible mushrooms. Food Chem. 2011, 128, 674–678.
25. Park, H.G.; Shim, Y.Y.; Choi, S.O.; Park, W.M. New method development for nanoparticle extraction of water-soluble β-(1→3)-D-glucan from edible mushrooms, Sparassis crispa and Phellinus linteus. J. Agric. Food Chem. 2009, 57, 2147–2154.
26. Ragaee, S.M.; Wood, P.J.; Wang, Q.; Tosh, S.M.; Brummer, Y.; Huang, X. Isolation, fractionation, and structural characteristics of alkali-extractable beta-glucan from rye whole meal. Cereal Chem. 2008, 85, 289–294.
27. Li, W.; Cui, S.W.; Kakuda, Y. Extraction, fractionation, structural and physical characterization of wheat β-D-glucans. Carbohydr. Polym. 2006, 63, 408–416.
28. Bao, X.F.; Fang, J.N.; Li, X.Y. Structural characterization and immunomodulating activity of a complex glucan from spores of Ganoderma lucidum. Biosci. Biotechnol. Biochem. 2001, 65, 2384–2391.
29. Ye, L.B.; Zhang, J.S.; Yang, Y.; Zhou, S.A.; Liu, Y.F.; Tang, Q.L.; Du, X.J.; Chen, H.; Pan, Y.J. Structural characterisation of a heteropolysaccharide by NMR spectra. Food Chem. 2009, 112, 962–966.
30. Johansson, L.; Virkki, L.; Anttila, H.; Esselstrom, H.; Tuomainen, P.; Sontag-Strohm, T. Hydrolysis of beta-glucan. Food Chem. 2006, 97, 71–79.
31. Blakeney, A.B.; Harris, P.J.; Henry, R.J.; Stone, B.A. A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohydr. Res. 1983, 113, 291–299.
32. Bernardo, D.; Mendoza, C.G.; Calonje, M.; Novaes-Ledieu, M. Chemical analysis of the lamella walls of Agaricus bisporus fruit bodies. Curr. Microbiol. 1999, 38, 364–367.
33. Ciucanu, I. Per-O-methylation reaction for structural analysis of carbohydrates by mass spectrometry. Anal. Chim. Acta 2006, 576, 147–155.
34. Agrawal, P.K. NMR spectroscopy in the structural elucidation of oligosaccharides and glycosides. Phytochemistry 1992, 31, 3307–3330.
35. Laws, A.P.; Chadha, M.J.; Chacon-Romero, M.; Marshall, V.M.; Maqsood, M. Determination of the structure and molecular weights of the exopolysaccharide produced by Lactobacillus acidophilus 5e2 when grown on different carbon feeds. Carbohydr. Res. 2008, 343, 301–307.
36. Wang, L.; Zhang, H.B.; Zhang, X.Y.; Chen, Z.X. Purification and identification of a novel heteropolysaccharide RBPS2a with anti-complementary activity from defatted rice bran. Food Chem. 2008, 110, 150–155.
37. Calonje, M.; García Mendoza, C.; Perez Cabo, A.; Novaes-Ledieu, M. New contributions to the wall polysaccharide structure of the vegetative mycelium and fruit body cell walls of Agaricus bisporus. Microbiología 1996, 12, 599–606.
38. Lee, J.S.; Kwon, J.S.; Won, D.P.; Lee, K.E.; Shin, W.C.; Hong, E.K. Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris. Carbohydr. Polym. 2010, 82, 982–988.
39. Yoshioka, Y.; Uehara, N.; Saito, H. Conformation-dependent change in antitumor-activity of linear and branched (1→3)-beta-D-glucans on the basis of conformational elucidation by C-13 Nuclear Magnetic Resonance spectroscopy. Chem. Pharm. Bull. 1992, 40, 1221–1226.
40. Kulicke, W.M.; Lettau, A.I.; Thielking, H. Correlation between immunological activity, molar mass, and molecular structure of different (1→3)-beta-D-glucans. Carbohydr. Res. 1997, 297, 135–143.
41. Jelsma, J.; Kreger, D.R. Ultrastructural observations on (1→3)-beta-D-glucan from fungal cell-walls. Carbohydr. Res. 1975, 43, 200–203.
42. Sweeley, C.C.; Nunez, H.A. Structural analysis of glycoconjugates by mass spectrometry and nuclear magnetic resonance spectroscopy. Annu. Rev. Biochem. 1985, 54, 765–801.
43. Young, S.H.; Jacobs, R.R. Sodium hydroxide-induced conformational change in schizophyllan detected by the fluorescence dye, aniline blue. Carbohydr. Res. 1998, 310, 91–99.
44. Ko, Y.T.; Lin, Y.L. 1,3-beta-glucan quantification by a fluorescence microassay and analysis of its distribution in foods. J. Agric. Food Chem. 2004, 52, 3313–3318.
45. Ogawa, K.; Dohmaru, T.; Yui, T. Dependence of complex-formation of (1→3)-beta-D-glucan with congo red on temperature in alkaline-solutions. Biosci. Biotechnol. Biochem. 1994, 58, 1870–1872.
46. Dore, C.; Azevedo, T.C.G.; de Souza, M.C.R.; Rego, L.A.; de Dantas, J.C.M.; Silva, F.R.F.; Rocha, H.A.O.; Basela, I.G.; Leite, E.L. Antiinflammatory, antioxidant and cytotoxic actions of beta-glucan-rich extract from Geastrum saecatum mushroom. Int. Immunopharmacol. 2007, 7, 1160–1169.
47. Smiderle, F.R.; Carbonero, E.R.; Sassaki, G.L.; Gorin, P.A.J.; Iacomini, M. Characterization of a heterogalactan: Some nutritional values of the edible mushroom Flammulina velutipes. Food Chem. 2008, 108, 329–333.
48. Numata, M.; Tamesue, S.; Fujisawa, T.; Haraguchi, S.; Hasegawa, T.; Bae, A.H.; Li, C.; Sakurai, K.; Shinkai, S. Beta-1,3-glucan polysaccharide (schizophyllan) acting as a one-dimensional host for creating supramolecular dye assemblies. Org. Lett. 2006, 8, 5533–5536.
49. Chakraborty, I.; Mondal, S.; Rout, D.; Islam, S.S. A water-insoluble (1→3)-beta-D-glucan from the alkaline extract of an edible mushroom Termitomyces eurhizus. Carbohydr. Res. 2006, 341, 2990–2993.
50. Chandra, K.; Ghosh, K.; Roy, S.K.; Mondal, S.; Maiti, D.; Ojha, A.K.; Das, D.; Islam, S.S. A water-soluble glucan isolated from an edible mushroom Termitomyces microcapus. Carbohydr. Res. 2007, 342, 2484–2489.
51. Dong, Q.; Jia, L.M.; Fang, J.N. A beta-D-glucan isolated from the fruiting bodies of Hericium erinaceus and its aqueous conformation. Carbohydr. Res. 2006, 341, 791–795.
52. Jia, L.M.; Liu, L.; Dong, Q.; Fang, J.N. Structural investigation of a novel rhamnoglucocalactan isolated from the fruiting bodies of the fungus Hericium erinaceus. Carbohydr. Res. 2004, 339, 2667–2671.
53. Wang, J.G.; Zhang, L. Structure and chain conformation of five water-soluble derivatives of a beta-D-glucan isolated from Ganoderma lucidum. Carbohydr. Res. 2009, 344, 105–112.
54. Dong, Q.; Wang, Y.; Shi, L.; Yao, J.; Li, J.; Ma, F.; Ding, K. A novel water-soluble beta-D-glucan isolated from the spores of Ganoderma lucidum. Carbohydr. Res. 2012, 353, 100–105.
55. Bao, X.F.; Wang, X.S.; Dong, Q.; Fang, J.N.; Li, X.Y. Structural features of immunologically active polysaccharides from Ganoderma lucidum. Phytochemistry 2002, 59, 175–181.
56. Chen, Y.; Xie, M.Y.; Nie, S.P.; Li, C.; Wang, Y.X. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem. 2008, 107, 231–241.
57. Xu, S.; Xu, X.; Zhang, L. Branching structure and chain conformation of water-soluble glucan extracted from Auricularia auricula-judae. J. Agric. Food Chem. 2012, 60, 3498–3506.
58. Nandan, C.K.; Patra, P.; Bhanja, S.K.; Adhikari, B.; Sarkar, R.; Mandal, S.; Islam, S.S. Structural characterization of a water-soluble beta-(1→6)-linked D-glucan isolated from the hot water extract of an edible mushroom, Agaricus bitorquis. Carbohydr. Res. 2008, 343, 3120–3122.
59. Gonzaga, M.L.C.; Ricardo, N.; Heatley, F.; Soares, S.D. Isolation and characterization of polysaccharides from Agaricus blazei Murill. Carbohydr. Polym. 2005, 60, 43–49.
60. Zhang, Y.Y.; Li, S.; Wang, X.H.; Zhang, L.N.; Cheung, P.C.K. Advances in lentinan: Isolation, structure, chain conformation and bioactivities. Food Hydrocoll. 2011, 25, 196–206.
61. Carbonero, E.R.; Gracher, A.H.P.; Komura, D.L.; Marcon, R.; Freitas, C.S.; Baggio, C.H.; Santos, A.R.S.; Torri, G.; Gorin, P.A.J.; Iacomini, M. Lentinus edodes heterogalactan: Antinociceptive and anti-inflammatory effects. Food Chem. 2008, 111, 531–537.
62. Palacios, I.; Guillamón, E.; García-Lafuente, A.; Villares, A. Structural characterization of water-soluble polysaccharides from the fruiting bodies of Lentinus edodes mushrooms. Curr. Nutr. Food Sci. 2012, 8, 234–241.
63. Karacsonyi, S.; Kuniak, L. Polysaccharides of Pleurotus ostreatus—Isolation and structure of pleuran, an alkali-insoluble β-D-glucan. Carbohydr. Polym. 1994, 24, 107–111.
64. Synytsya, A.; Mickova, K.; Jablonsky, I.; Spevacek, J.; Erban, V.; Kovarikova, E.; Copikova, J. Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii: Structure and potential prebiotic activity. Carbohydr. Polym. 2009, 76, 548–556.
65. Palacios, I.; García-Lafuente, A.; Guillamón, E.; Villares, A. Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms. Carbohydr. Res. 2012, 358, 72–77.
66. Santos-Neves, J.C.; Pereira, M.I.; Carbonero, E.R.; Gracher, A.H.P.; Alquini, G.; Gorin, P.A.J.; Sassaki, G.L.; Iacomini, M. A novel branched alpha beta-glucan isolated from the basidiocarps of the edible mushroom Pleurotus florida. Carbohydr. Polym. 2008, 73, 309–314.
67. Rout, D.; Mondal, S.; Chakraborty, I.; Islam, S.S. The structure and conformation of a water-insoluble (1→3),(1→6)-beta-D-glucan from the fruiting bodies of Pleurotus florida. Carbohydr. Res. 2008, 343, 982–987.
68. Carbonero, E.R.; Gracher, A.H.P.; Rosa, M.C.C.; Torri, G.; Sassaki, G.L.; Gorin, P.A.J.; Iacomini, M. Unusual partially 3-O-methylated alpha-galactan from mushrooms of the genus Pleurotus. Phytochemistry 2008, 69, 252–257.
69. Rosado, F.R.; Carbonero, E.R.; Kemmelmeier, C.; Tischer, C.A.; Gorin, P.A.J.; Iacomini, M. A partially 3-O-methylated (1→4)-linked α-D-galactan and α-D-mannan from Pleurotus ostreatoroseus Sing. FEMS Microbiol. Lett. 2002, 212, 261–265.
70. Mandal, E.K.; Maity, K.; Maity, S.; Gantait, S.K.; Behera, B.; Maiti, T.K.; Sikdar, S.R.; Islam, S.S. Chemical analysis of an immunostimulating (1→4)-, (1→6)-branched glucan from an edible mushroom, Calocybe indica. Carbohydr. Res. 2012, 347, 172–177.
71. Mandal, S.; Maity, K.K.; Bhunia, S.K.; Dey, B.; Patra, S.; Sikdar, S.R.; Islam, S.S. Chemical analysis of new water-soluble (1→6)-, (1→4)-α, β-glucan and water-insoluble (1→3)-, (1→4)-β-glucan (calocyban) from alkaline extract of an edible mushroom, Calocybe indica (Dudh Chattu). Carbohydr. Res. 2010, 345, 2657–2663.
72. Mandal, E.K.; Maity, K.; Maity, S.; Gantait, S.K.; Maiti, S.; Maiti, T.K.; Sikdar, S.R.; Islam, S.S. Structural characterization of an immunoenhancing cytotoxic heteroglycan isolated from an edible mushroom Calocybe indica var. APK2. Carbohydr. Res. 2011, 346, 2237–2243.
73. Chauveau, C.; Talaga, P.; Wieruszeski, J.M.; Strecker, G.; Chavant, L. A water-soluble β-D-glucan from Boletus erythropus. Phytochemistry 1996, 43, 413–415.
74. Yue, L.; Cui, H.; Li, C.; Lin, Y.; Sun, Y.; Niu, Y.; Wen, X.; Liu, J. A polysaccharide from Agaricus blazei attenuates tumor cell adhesion via inhibiting E-selectin expression. Carbohydr. Polym. 2012, 88, 1326–1333.
75. Hsieh, T.-C.; Wu, J.M. Suppression of proliferation and oxidative stress by extracts of Ganoderma lucidum in the ovarian cancer cell line OVCAR-3. Int. J. Mol. Med. 2011, 28, 1065–1069.
76. Lee, K.H.; Cho, C.H.; Rhee, K.-H. Synergic anti-tumor activity of gamma-irradiated exo-polysaccharide from submerged culture of Grifola frondosa. J. Med. Plants Res. 2011, 5, 2378–2386.
77. Selvi, S.; Umadevi, P.; Murugan, S.; Senapathy, G.J. Anticancer potential evoked by Pleurotus florida and Calocybe indica using T24 urinary bladder cancer cell line. Afr. J. Biotechnol. 2011, 10, 7279–7285.
78. Zhang, L.; Fan, C.; Liu, S.; Zang, Z.; Jiao, L.; Zhang, L. Chemical composition and antitumor activity of polysaccharide from Inonotus obliquus. J. Med. Plants Res. 2011, 5, 1251–1260.
79. Lo, T.C.-T.; Hsu, F.-M.; Chang, C.A.; Cheng, J.C.-H. Branched alpha-(1,4) glucans from Lentinula edodes (L10) in combination with radiation enhance cytotoxic effect on human lung adenocarcinoma through the toll-like receptor 4 mediated induction of THP-1 differentiation/activation. J. Agric. Food Chem. 2011, 59, 11997–12005.
80. Wang, G.; Dong, L.; Zhang, Y.; Ji, Y.; Xiang, W.; Zhao, M. Polysaccharides from Phellinus linteus inhibit cell growth and invasion and induce apoptosis in HepG2 human hepatocellular carcinoma cells. Biologia 2012, 67, 247–254.
81. Hu, Q.; Wang, H.; Ng, T.B. Isolation and purification of polysaccharides with anti-tumor activity from Pholiota adiposa (Batsch) P. Kumm. (higher Basidiomycetes). Int. J. Med. Mushrooms 2012, 14, 271–284.
82. Chihara, G.; Hamuro, J.; Maeda, Y.Y.; Shiio, T.; Suga, T.; Takasuka, N.; Sasaki, T. Antitumor and metastasis-inhibitory activities of lentinan as an immunomodulator: An overview. Cancer Detect. Prev. 1987, 1, 423–443.
83. Ochiai, T.; Isono, K.; Suzuki, T.; Koide, Y.; Gunji, Y.; Nagata, M.; Ogawa, N. Effect of immunotherapy with lentinan on patients survival and immunological parameters in patients with advanced gastric-cancer—Results of a multicenter randomized controlled-study. Int. J. Immunother. 1992, 8, 161–169.
84. Taguchi, T.; Abe, O.; Enomoto, K.; Kusama, S.; Tomiyama, J.; Tominaga, K.; Ogawa, N. Life-span prolongation effect of lentinan on patients with advanced or recurrent breast-cancer. Int. J. Immunopharmacol. 1982, 4, 271–271.
85. Kosaka, A.; Suga, T.; Yamashita, A. Dose reductive effect of lentinan on the epirubicin therapy for breast cancer patients. Int. J. Immunother. 1995, 11, 143–151.
86. Hamuro, J.; Takatsuki, F.; Suga, T.; Kikuchi, T.; Suzuki, M. Synergistic antimetastatic effects of lentinan and interleukin-2 with preoperative and postoperative treatments. Jpn. J. Cancer Res. 1994, 85, 1288–1297.
87. Shimizu, C.; Kihara, M.; Aoe, S.; Araki, S.; Ito, K.; Hayashi, K.; Watari, J.; Sakata, Y.; Ikegami, S. Effect of high beta-glucan barley on serum cholesterol concentrations and visceral fat area in japanese men—A randomized, double-blinded, placebo-controlled trial. Plant Food Hum. Nutr. 2008, 63, 21–25.
88. Naumann, E.; van Rees, A.B.; Onning, G.; Oste, R.; Wydra, M.; Mensink, R.P. Beta-glucan incorporated into a fruit drink effectively lowers serum LDL-cholesterol concentrations. Am. J. Clin. Nutr. 2006, 83, 601–605.
89. Drozdowski, L.A.; Reimer, R.A.; Temelli, F.; Bell, R.C.; Vasanthan, T.; Thomson, A.B.R. β-glucan extracts inhibit the in vitro intestinal uptake of long-chain fatty acids and cholesterol and down-regulate genes involved in lipogenesis and lipid transport in rats. J. Nutr. Biochem. 2010, 21, 695–701.
90. Gordon, M.; Guralnik, M.; Kaneko, Y.; Mimura, T.; Goodgame, J.; DeMarzo, C.; Pierce, D.; Baker, M.; Lang, W. A phase II controlled study of a combination of the immune modulator, lentinan, with didanosine (ddi) in HIV patients with CD4 cells of 200–500/mm3. J. Med. 1995, 26, 193–207.
91. Drandarska, I.; Kussovski, V.; Nikolaeva, S.; Markova, N. Combined immunomodulating effects of BCG and lentinan after intranasal application in guinea pigs. Int. Immunopharmacol. 2005, 5, 795–803.
92. Asatiani, M.D.; Elisashvili, V.I.; Wasser, S.P.; Reznick, A.Z.; Nevo, E. Free-radical scavenging activity of submerged mycelium extracts from higher basidiomycetes mushrooms. Biosci. Biotechnol. Biochem. 2007, 71, 3090–3092.
93. Asatiani, M.D.; Elisashvili, V.; Wasser, S.P.; Reznick, A.Z.; Nevo, E. Antioxidant activity of submerged cultured mycelium extracts of higher basidiomycetes mushrooms. Int. J. Med. Mushrooms 2007, 9, 151–158.
94. Heleno, S.A.; Barros, L.; Martins, A.; Queiroz, M.J.R.P.; Santos-Buelga, C.; Ferreira, I.C.F.R. Phenolic, polysaccharidic, and lipidic fractions of mushrooms from northeastern Portugal: Chemical compounds with antioxidant properties. J. Agric. Food Chem. 2012, 60, 4634–4640.
95. Wang, C.; Chen, Y.; Hu, M.; Ding, J.; Xu, C.; Wang, R. In vitro antioxidant activities of the polysaccharides from Tricholoma lobayense. Int. J. Biol. Macromol. 2012, 50, 534–539.
96. Kao, P.-F.; Wang, S.-H.; Hung, W.-T.; Liao, Y.-H.; Lin, C.-M.; Yang, W.-B. Structural characterization and antioxidative activity of low-molecular-weights β-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies. J. Biomed. Biotechnol. 2012, doi:10.1155/2012/673764.
97. Li, N.; Li, L.; Fang, J.C.; Wong, J.H.; Ng, T.B.; Jiang, Y.; Wang, C.R.; Zhang, N.Y.; Wen, T.Y.; Qu, L.Y., et al. Isolation and identification of a novel polysaccharide-peptide complex with antioxidant, anti-proliferative and hypoglycaemic activities from the abalone mushroom. Biosci. Rep. 2012, 32, 221–228.
98. Liatis, S.; Tsapogas, P.; Chala, E.; Dimosthenopoulos, C.; Kyriakopoulos, K.; Kapantais, E.; Katsilambros, N. The consumption of bread enriched with betaglucan reduces LDL-cholesterol and improves insulin resistance in patients with type 2 diabetes. Diabetes Metab. 2009, 35, 115–120.
99. Barone Lumaga, R.; Azzali, D.; Fogliano, V.; Scalfi, L.; Vitaglione, P. Sugar and dietary fibre composition influence, by different hormonal response, the satiating capacity of a fruit-based and a β-glucan-enriched beverage. Food Funct. 2012, 3, 67–75.
100. Ye, M.; Chen, W.-X.; Qiu, T.; Yuan, R.-Y.; Ye, Y.-W.; Cai, J.-M. Structural characterisation and anti-ageing activity of extracellular polysaccharide from a strain of Lachnum sp. Food Chem. 2012, 132, 338–343.
101. Velebny, S.; Hrckova, G.; Kogan, G. Impact of treatment with praziquantel, silymarin and/or β-glucan on pathophysiological markers of liver damage and fibrosis in mice infected with Mesocestoides vogae (Cestoda) tetrathyridia. J. Helminthol. 2008, 82, 211–219.