Functional expression and characterisation of membrane transport proteins

Plant Biology

Volumn 14, Issue 5, 2012, Pages 675-690

  Haferkamp, I ^a   Linka, N ^b  

^a Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Electrophysiological techniques; Flux assays; Heterologous expression systems; Membrane protein purification; Membrane transporter

Indexed keywords

GENE EXPRESSION; GENOME; MEMBRANE; METABOLISM; METABOLITE; PROTEIN; PURIFICATION; SUBSTRATE; TRANSLOCATION;

CARRIER PROTEIN; VEGETABLE PROTEIN;

GENE EXPRESSION; ISOLATION AND PURIFICATION; METABOLISM;

GENE EXPRESSION; MEMBRANE TRANSPORT PROTEINS; PLANT PROTEINS;

EID: 84864753527     PISSN: 14358603     EISSN: 14388677     Source Type: Journal    
DOI: 10.1111/j.1438-8677.2012.00591.x     Document Type: Review

References (240)

1. Abel S., Theologis A. (1994) Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. Plant Journal, 5, 421-424.
2. Ai P., Sun S., Zhao J., Fan X., Xin W., Guo Q., Yu L., Shen Q., Wu P., Miller A.J., Xu G. (2009) Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation. Plant Journal, 57, 798-809.
3. van Aken T., Foxall-Van Aken S., Castleman S., Ferguson-Miller S. (1986) Alkyl glycoside detergents - synthesis and applications to the study of membrane proteins. Methods in Enzymology, 125, 27-35.
4. Alderson A., Sabelli P.A., Dickinson J.R., Cole D., Richardson M., Kreis M., Shewry P.R., Halford N.G. (1991) Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein kinase cDNA. Proceedings of the National Academy of Sciences USA, 88, 8602-8605.
5. Allen T.M., Romans A.Y., Kercret H., Segrest J.P. (1980) Detergent removal during membrane reconstitution. Biochimica et Biophysica Acta, 601, 328-342.
6. +-ATPase into phosphatidylcholine vesicles by dialysis of nonionic alkyl maltoside detergents. Biochimica et Biophysica Acta, 946, 379-388.
7. Aluri S., Buttner M. (2007) Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and flowering. Proceedings of the National Academy of Sciences USA, 104, 2537-2542.
8. +/hexose cotransporter in Xenopus oocytes and its characterization with respect to sugar specificity, pH and membrane-potential. Journal of Plant Physiology, 141, 293-297.
9. Ast M., Gruber A., Schmitz-Esser S., Neuhaus H.E., Kroth P.G., Horn M., Haferkamp I. (2009) Diatom plastids depend on nucleotide import from the cytosol. Proceedings of the National Academy of Sciences USA, 106, 3621-3626.
10. Bakker H., Routier F., Oelmann S., Jordi W., Lommen A., Gerardy-Schahn R., Bosch D. (2005) Molecular cloning of two Arabidopsis UDP-galactose transporters by complementation of a deficient Chinese hamster ovary cell line. Glycobiology, 15, 193-201.
11. Baldi L., Hacker D.L., Adam M., Wurm F.M. (2007) Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives. Biotechnology Letters, 29, 677-684.
12. Baldwin T.C., Handford M.G., Yuseff M.I., Orellana A., Dupree P. (2001) Identification and characterization of GONST1, a Golgi-localized GDP-mannose transporter in Arabidopsis. Plant Cell, 13, 2283-2295.
13. Barth I., Meyer S., Sauer N. (2003) PmSUC3: characterization of a SUT2/SUC3-type sucrose transporter from Plantago major. Plant Cell, 15, 1375-1385.
14. Bassham D.C., Raikhel N.V. (2000) Plant cells are not just green yeast. Plant Physiology, 122, 999-1001.
15. 2+-dependent manner. Proceedings of the National Academy of Sciences USA, 101, 15621-15626.
16. Bedhomme M., Hoffmann M., McCarthy E.A., Gambonnet B., Moran R.G., Rébeillé F., Ravanel S. (2005) Folate metabolism in plants: an Arabidopsis homolog of the mammalian mitochondrial folate transporter mediates folate import into chloroplasts. Journal of Biological Chemistry, 280, 34823-34831.
17. Bergsdorf E.Y., Zdebik A.A., Jentsch T.J. (2009) Residues important for nitrate/proton coupling in plant and mammalian CLC transporters. Journal of Biological Chemistry, 284, 11184-11193.
18. Bernaudat F., Frelet-Barrand A., Pochon N., Dementin S., Hivin P., Boutigny S., Rioux J.B., Salvi D., Seigneurin-Berny D., Richaud P., Joyard J., Pignol D., Sabaty M., Desnos T., Pebay-Peyroula E., Darrouzet E., Vernet T., Rolland N. (2011) Heterologous expression of membrane proteins: choosing the appropriate host. PLoS ONE, 6, e29191.
19. + and contributes to optimal fatty acid degradation during storage oil mobilisation. Plant Journal, 69, 1-13.
20. Berrier C., Park K.H., Abes S., Bibonne A., Betton J.M., Ghazi A. (2004) Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. Biochemistry, 43, 12585-12591.
21. + channel from Arabidopsis thaliana, and comparison with endogeneous yeast channels and carriers. Proceedings of the National Academy of Sciences USA, 92, 2701-2705.
22. Bessette P.H., Aslund F., Beckwith J., Georgiou G. (1999) Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proceedings of the National Academy of Sciences USA, 96, 13703-13708.
23. Bigay J., Gounon P., Robineau S., Antonny B. (2003) Lipid packing sensed by ArfGAP1 couples COPI coat disassembly to membrane bilayer curvature. Nature, 426, 563-566.
24. Birch R.G., Franks T. (1991) Development and optimization of microprojectile systems for plant genetic transformation. Australian Journal of Plant Physiology., 18, 453-469.
25. Bolter B., Soll J., Hill K., Hemmler R., Wagner R. (1999) A rectifying ATP-regulated solute channel in the chloroplastic outer envelope from pea. EMBO Journal, 18, 5505-5516.
26. Boorer K.J., Forde B.G., Leigh R.A., Miller A.J. (1992) Functional expression of a plant plasma membrane transporter in Xenopus oocytes. FEBS Letters, 302, 166-168.
27. Bouvier F., Linka N., Isner J.C., Mutterer J., Weber A.P.M., Camara B. (2006) Arabidopsis SAMT1 defines a plastid transporter regulating plastid biogenesis and plant development. Plant Cell, 18, 3088-3105.
28. Brink S., Flügge U.I., Chaumont F., Boutry M., Emmermann M., Schmitz U., Becker K., Pfanner N. (1994) Preproteins of chloroplast envelope inner membrane contain targeting information for receptor-dependent import into fungal mitochondria. Journal of Biological Chemistry, 269, 16478-16485.
29. + channels and KAT1 and KST1 alpha-subunit homomers of guard cells. Planta, 207, 370-376.
30. Carpaneto A., Geiger D., Bamberg E., Sauer N., Fromm J., Hedrich R. (2005) Phloem-localized proton-coupled sucrose carrier ZmSUT1 mediates sucrose efflux under the control of the sucrose gradient and the proton motive force. Journal of Biological Chemistry, 280, 21437-21443.
31. Carrier D.J., Bakar N.T.A., Swarup R., Callaghan R., Napier R.M., Bennett M.J., Kerr I.D. (2008) The binding of auxin to the Arabidopsis auxin influx transporter AUX1. Plant Physiology, 148, 529-535.
32. Cereghino J.L., Cregg J.M. (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiology Reviews, 24, 45-66.
33. Cereghino G.P., Cereghino J.L., Ilgen C., Cregg J.M. (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Current Opinion in Biotechnolog, 13, 329-332.
34. Chaumont F., Barrieu F., Jung R., Chrispeels M.J. (2000) Plasma membrane intrinsic proteins from maize cluster in two sequence subgroups with differential aquaporin activity. Plant Physiology, 122, 1025-1034.
35. Chen L.Q., Hou B.H., Lalonde S., Takanga H., Hartung M.L., Qu X.Q., Guo W.J., Kim J.G., Underwood W., Chaudhuri B., Chermak D., Antony G., White S.C., Somerville S.C., Mudgett M.B., Frommer W.B. (2010) Sugar transporters for intercellular exchange and nutrition of pathogens. Nature, 468, 527-532.
36. Chen L.Q., Qu X.Q., Hou B.H., Sosso D., Osorio S., Fernie A.R., Frommer W.B. (2012) Sucrose efflux mediated by SWEET proteins as a key step for phloem transport. Science, 335, 207-211.
37. Chiang C.S., Stacey G., Tsay Y.F. (2004) Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2. Journal of Biological Chemistry, 279, 30150-30157.
38. Choi W.G., Roberts D.M. (2007) Arabidopsis NIP2;1, a major intrinsic protein transporter of lactic acid induced by anoxic stress. Journal of Biological Chemistry, 282, 24209-24218.
39. Clough S.J., Bent A.F. (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant Journal, 16, 735-743.
40. Cohen F.S., Niles W.D., Akabas M.H. (1989) Fusion of phospholipid vesicles with a planar membrane depends on the membrane permeability of the solute used to create the osmotic pressure. Journal of General Physiology, 93, 201-210.
41. Colmenero-Flores J.M., Martínez G., Gamba G., Vázquez N., Iglesias D.J., Brumós J., Talón M. (2007) Identification and functional characterization of cation-chloride cotransporters in plants. Plant Journal, 50, 278-292.
42. Colosimo A., Goncz K.K., Holmes A.R., Kunzelmann K., Novelli G., Malone R.W., Bennett M.J., Gruenert D.C. (2000) Transfer and expression of foreign genes in mammalian cells. BioTechniques, 29, 314-318, 320-2, 324.
43. Darszon A. (1986) Planar bilayers: a powerful tool to study membrane proteins involved in ion transport. Methods in Enzymology, 127, 486-502.
44. DasSarma S., Tischer E., Goodman H.M. (1986) Plant glutamine synthetase complements a glnA mutation in Escherichia coli. Science, 232, 1242-1244.
45. Deisenhofer J., Epp O., Miki K., Huber R., Michel H. (1984) X-ray structure analysis of a membrane protein complex. Electron density map at 3 Å resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis. Journal of Molecular Biololgy, 180, 385-398.
46. Deniaud A., Bernaudat F., Frelet-Barrand A., Juillan-Binard C., Vernet T., Rolland N., Pebay-Peyroula E. (2011) Expression of a chloroplast ATP/ADP transporter in E. coli membranes: behind the Mistic strategy. Biochimica et Biophysica Acta, 1808, 2059-2066.
47. DeVries J.K., Zubay G. (1967) DNA-directed peptide synthesis. II. The synthesis of the alpha-fragment of the enzyme beta-galactosidase. Proceedings of the National Academy of Sciences USA, 57, 1010-1012.
48. Diller A., Loudet C., Aussenac F., Raffard G., Fournier S., Laguerre M., Grelard A., Opella S.J., Marassi F.M., Dufourc E.J. (2009) Bicelles: A natural 'molecular goniometer' for structural, dynamical and topological studies of molecules in membranes. Biochimie, 91, 744-751.
49. Drew D., Fröderberg L., Baars L., de Gier J.W. (2003) Assembly and overexpression of membrane proteins in Escherichia coli. Biochimica et Biophysica Acta, 1610, 3-10.
50. Drew D., Lerch M., Kunji E., Slotboom D.J., de Gier J.W. (2006) Optimization of membrane protein overexpression and purification using GFP fusions. Nature Methods, 3, 303-313.
51. Drew D., Newstead S., Sonoda Y., Kim H., von Heijne G., Iwata S. (2008) GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nature Protocols, 3, 784-798.
52. Dreyer I., Horeau C., Lemaillet G., Zimmermann S., Bush D.R., Rodriguez-Navarro A., Schachtman D.P., Spalding E.P., Sentenac H., Gaber R.F. (1999) Identification and characterization of plant transporters using heterologous expression systems. Journal of Experimental Botany, 50, 1073-1087.
53. Dvir H., Choe S. (2009) Bacterial expression of a eukaryotic membrane protein in fusion to various Mistic orthologs. Protein Expression and Purification, 68, 28-33.
54. Eicks M., Maurino V., Knappe S., Flügge U.I., Fischer K. (2002) The plastidic pentose phosphate translocator represents a link between the cytosolic and the plastidic pentose phosphate pathways in plants. Plant Physiology, 128, 512-522.
55. Elbaz Y., Steiner-Mordoch S., Danieli T., Schuldiner S. (2004) In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state. Proceedings of the National Academy of Sciences USA, 101, 1519-1524.
56. Endo Y., Sawasaki T. (2006) Cell-free expression systems for eukaryotic protein production. Current Opinion in Biotechnology, 17, 373-380.
57. Fang H., Zhang X., Shen L., Si X., Ren Y., Dai H., Li S., Zhou C., Chen H. (2009) RimJ is responsible for N(alpha)-acetylation of thymosin alpha1 in Escherichia coli. Applied Microbiology and Biotechnology, 84, 99-104.
58. Fertig N., Meyer C., Blick R.H., Trautmann C., Behrends J.C. (2001) Microstructured glass chip for ion-channel electrophysiology. Physical Review E Statistical, Nonlinear, and Soft Matter Physics, 64, 040901.
59. Fertig N., Klau M., George M., Blick R.H., Behrends J.C. (2002a) Activity of single ion channel proteins detected with a planar microstructure. Applied Physics Letters, 81, 4865-4867.
60. Fertig N., Blick R.H., Behrends J.C. (2002b) Whole cell patch clamp recording performed on a planar glass chip. Biophysical Journal, 82, 3056-3062.
61. Fischer K., Kammerer B., Gutensohn M., Arbinger B., Weber A., Häusler R.E., Flügge U.I. (1997) A new class of plastidic phosphate translocators: a putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter. Plant Cell, 9, 453-462.
62. Flügge U.I. (1992) Reaction mechanism and asymmetric orientation of the reconstituted chloroplast phosphate translocator. Biochimica et Biophysica Acta, 1110, 112-118.
63. Flügge U.I., Gao W. (2005) Transport of isoprenoid intermediates across chloroplast envelope membranes. Plant Biology, 7, 91-97.
64. Frommer W.B., Ninnemann O. (1995) Heterologous expression of genes in bacterial, fungal, animal, and plant cells. Annual Review of Plant Physiology and Plant Molecular Biology, 46, 419-444.
65. + transporter from Arabidopsis. Plant Cell, 10, 63-73.
66. Gale E.F., Folkes J.P. (1954) Effect of nucleic acids on protein synthesis and amino acid incorporation in disrupted staphylococcal cells. Nature, 173, 1223-1227.
67. Galian C., Maon F., Dezi M., Torres C., Ebel C., Levy D., Jault J.M. (2011) Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2-D crystallization. PLoS ONE, 6, e19677.
68. Garcia-Mata R., Bebök Z., Sorscher E.J., Sztul E.S. (1999) Characterization and dynamics of aggresome formation by a cytosolic GFP-chimera. Journal of Cell Biology, 20, 1239-1254.
69. Gaymard F., Cerutti M., Horeau G., Lemaillet G., Urbach S., Ravallec M., Devauchelle G., Sentenac H., Thibaud J.B. (1996) The baculovirus/insect cell system as an alternative to Xenopus oocytes. First characterization of the AKT1 K+ channel from Arabidopsis thaliana. Journal of Biological Chemistry, 271, 22863-22870.
70. Geisse S., Gram H., Kleuser B., Kocher H.P. (1996) Eukaryotic expression systems: a comparison. Protein Expression and Purification, 8, 271-282.
71. Giaever G., Chu A.M., Ni L., Connelly C., Riles L., Véronneau S., Dow S., Lucau-Danila A., Anderson K., André B., Arkin A.P., Astromoff A., El-Bakkoury M., Bangham R., Benito R., Brachat S., Campanaro S., Curtiss M., Davis K., Deutschbauer A., Entian K.D., Flaherty P., Foury F., Garfinkel D.J., Gerstein M., Gotte D., Güldener U., Hegemann J.H., Hempel S., Herman Z., Jaramillo D.F., Kelly D.E., Kelly S.L., Kötter P., LaBonte D., Lamb D.C., Lan N., Liang H., Liao H., Liu L., Luo C., Lussier M., Mao R., Menard P., Ooi S.L., Revuelta J.L., Roberts C.J., Rose M., Ross-Macdonald P., Scherens B., Schimmack G., Shafer B., Shoemaker D.D., Sookhai-Mahadeo S., Storms R.K., Strathern J.N., Valle G., Voet M., Volckaert G., Wang C.Y., Ward T.R., Wilhelmy J., Winzeler E.A., Yang Y., Yen G., Youngman E., Yu K., Bussey H., Boeke J.D., Snyder M., Philippsen P., Davis R.W., Johnston M. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418, 387-391.
72. Goff S.A., Casson L.P., Goldberg A.L. (1984) Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli. Proceedings of the National Academy of Sciences USA, 81, 6647-6651.
73. Goffeau A., Barrell B.G., Bussey H., Davis R.W., Dujon B., Feldmann H., Galibert F., Hoheisel J.D., Jacq C., Johnston M., Louis E.J., Mewes H.W., Murakami Y., Philippsen P., Tettelin H., Oliver S.G. (1996) Life with 6000 genes. Science, 274, 546, 563-546, 567.
74. Goren M.A., Fox B.G. (2008) Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. Protein Expression and Purification, 62, 171-178.
75. Götze T.A., Philippar K., Ilkavets I., Soll J., Wagner R. (2006) OEP37 is a new member of the chloroplast outer membrane ion channels. Journal of Biological Chemistry, 281, 17989-17998.
76. Gourdon P., Alfredsson A., Pedersen A., Malmerberg E., Nyblom M., Widell M., Berntsson R., Pinhassi J., Braiman M., Hansson O., Bonander N., Karlsson G., Neutze R. (2008) Optimized in vitro and in vivo expression of proteorhodopsin: a seven-transmembrane proton pump. Protein Expression and Purification, 58, 103-113.
77. Guo B., Jin Y., Wussler C., Blancaflor E.B., Motes C.M., Versaw W.K. (2008) Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters. New Phytologist, 177, 889-898.
78. Gurdon J.B., Lane C.D., Woodland H.R., Marbaix G. (1971) Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature, 233, 177-182.
79. Haferkamp I., Hackstein J.H., Voncken F.G., Schmit G., Tjaden J. (2002) Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids. European Journal of Biochemistry, 269, 3172-3181.
80. + transporter in an intracellular bacterial symbiont related to Chlamydiae. Nature, 432, 622-625.
81. Haferkamp I., Schmitz-Esser S., Wagner M., Neigel N., Horn M., Neuhaus H.E. (2006) Tapping the nucleotide pool of the host: novel nucleotide carrier proteins of Protochlamydia amoebophila. Molecular Microbiology, 60, 1534-1545.
82. + channel using yeast tonoplasts. Journal of Biological Chemistry, 283, 1911-1920.
83. Hamilton A.J., Bouzayen M., Grierson D. (1991) Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast. Proceedings of the National Academy of Sciences USA, 88, 7434-7437.
84. Hedrich R., Kurkdjian A. (1988) Characterization of an anion-permeable channel from sugar beet vacuoles: effect of inhibitors. EMBO Journal, 7, 3661-3666.
85. Heimpel S., Basset G., Odoy S., Klingenberg M. (2001) Expression of the mitochondrial ADP/ATP carrier in Escherichia coli. Renaturation, reconstitution, and the effect of mutations on 10 positive residues. Journal of Biological Chemistry, 276, 11499-11506.
86. Henikoff S., Tatchell K., Hall B.D., Nasmyth K.A. (1981) Isolation of a gene from Drosophila by complementation in yeast. Nature, 289, 33-37.
87. Herick K., Kramer R., Luhring H. (1997) Patch clamp investigation into the phosphate carrier from Saccharomyces cerevisiae mitochondria. Biochimica et Biophysica Acta, 1321, 207-220.
88. Hodgkin A.L., Huxley A.F., Katz B. (1952) Measurement of current-voltage relations in the membrane of the giant axon of Loligo. Journal of Physiology (London), 116, 424-448.
89. Hoffmann B., Stöckl A., Schlame M., Beyer K., Klingenberg M. (1994) The reconstituted ADP/ATP carrier activity has an absolute requirement for cardiolipin as shown in cysteine mutants. Journal of Biological Chemistry, 269, 1940-1944.
90. Hohe A., Decker E.L., Gorr G., Schween G., Reski R. (2002) Tight control of growth and cell differentiation in photoautotrophically growing moss Physcomitrella patens bioreactor cultures. Plant Cell Reports, 20, 1135-1140.
91. Hoyos M.E., Palmieri L., Wertin T., Arrigoni R., Polacco J.C., Palmieri F. (2003) Identification of a mitochondrial transporter for basic amino acids in Arabidopsis thaliana by functional reconstitution into liposomes and complementation in yeast. Plant Journal, 33, 1027-1035.
92. Ikeda M., Arai M., Lao D.M., Shimizu T. (2002) Transmembrane topology prediction methods: a re-assessment and improvement by a consensus method using a dataset of experimentally-characterized transmembrane topologies. In Silico Biology, 2, 19-33.
93. Itakura K., Hirose T., Crea R., Riggs A.D., Heyneker H.L., Bolivar F., Boyer H.W. (1977) Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science, 198, 1056-1063.
94. Jarvis D.L. (2009) Baculovirus-insect cell expression systems. Methods in Enzymology, 463, 191-222.
95. Jiang F., Ryan M.T., Schlame M., Zhao M., Gu Z., Klingenberg M., Pfanner N., Greenberg M.L. (2000) Absence of cardiolipin in the crd1 null mutant results in decreased mitochondrial membrane potential and reduced mitochondrial function. Journal of Biological Chemistry, 275, 22387-22394.
96. Johnson M., Coulton A.T., Geeves M.A., Mulvihill D.P. (2010) Targeted amino-terminal acetylation of recombinant proteins in E. coli. PLoS ONE, 5, e15801.
97. Jost W., Link S., Horstmann V., Decker E.L., Reski R., Gorr G. (2005) Isolation and characterisation of three moss-derived beta-tubulin promoters suitable for recombinant expression. Current Genetics, 47, 111-120.
98. Junge F., Schneider B., Reckel S., Schwarz D., Dötsch V., Bernhard F. (2008) Large-scale production of functional membrane proteins. Cellular and Molecular Life Sciences, 65, 1729-1755.
99. Kaiser L., Graveland-Bikker J., Steuerwald D., Vanberghem M., Herlihy K., Zhang S. (2008) Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses. Proceedings of the National Academy of Sciences USA, 105, 15726-15731.
100. Kalmbach R., Chizhov I., Schumacher M.C., Friedrich T., Bamberg E., Engelhard M. (2007) Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomes. Journal of Molecular Biology, 371, 639-648.
101. Kammerer B., Fischer K., Hilpert B., Schubert S., Gutensohn M., Weber A., Flügge U.I. (1998) Molecular characterization of a carbon transporter in plastids from heterotrophic tissues: The glucose-6-phosphate/phosphate antiporter. Plant Cell, 10, 105-117.
102. Kane J.F. (1995) Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Current Opinion in Biotechnology, 6, 494-500.
103. Kasahara M., Hinkle P.C. (1977) Reconstitution and purification of the D-glucose transporter from human erythrocytes. Journal of Biological Chemistry, 252, 7384-7390.
104. Keller T., Schwarz D., Bernhard F., Dötsch V., Hunte C., Gorboulev V., Koepsell H. (2008) Cell-free expression and functional reconstitution of eukaryotic drug transporters. Biochemistry, 47, 4552-4564.
105. Kigawa T., Yabuki T., Matsuda N., Matsuda T., Nakajima R., Tanaka A., Yokoyama S. (2004) Preparation of Escherichia coli cell extract for highly productive cell-free protein expression. Journal of Structural and Functional Genomics, 5, 63-68.
106. Kirchberger S., Leroch M., Huynen M.A., Wahl M., Neuhaus H.E., Tjaden J. (2007) Molecular and biochemical analysis of the plastidic ADP-glucose transporter (ZmBT1) from Zea mays. Journal of Biological Chemistry, 282, 22481-22491.
107. Klammt C., Schwarz D., Fendler K., Haase W., Dötsch V., Bernhard F. (2005) Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS Journal, 272, 6024-6038.
108. +-symport of numerous substrates, including myo-inositol, glycerol, and ribose. Plant Cell, 17, 204-218.
109. + symporters in pollen and young xylem cells. Journal of Experimental Botany, 61, 537-550.
110. Klingenberg M. (2008) The ADP and ATP transport in mitochondria and its carrier. Biochimica et Biophysica Acta, 1778, 1978-2021.
111. Knappe S., Löttgert T., Schneider A., Voll L., Flügge U.I., Fischer K. (2003) Characterization of two functional phosphoenolpyruvate/phosphate translocator (PPT) genes in Arabidopsis - AtPPT1 may be involved in the provision of signals for correct mesophyll development. Plant Journal, 36, 411-420.
112. Knol J., Sjollema K., Poolman B. (1998) Detergent-mediated reconstitution of membrane proteins. Biochemistry, 37, 16410-16415.
113. Kovermann P., Meyer S., Hortensteiner S., Picco C., Scholz-Starke J., Ravera S., Lee Y., Martinoia E. (2007) The Arabidopsis vacuolar malate channel is a member of the ALMT family. Plant Journal, 52, 1169-1180.
114. Kunji E.R., Chan K.W., Slotboom D.J., Floyd S., O'Connor R., Monne M. (2005) Eukaryotic membrane protein overproduction in Lactococcuslactis. Current Opinion in Biotechnology, 16, 546-551.
115. Kuromori T., Miyaji T., Yabuuchi H., Shimizu H., Sugimoto E., Kamiya A., Moriyama Y., Shinozaki K. (2010) ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proceedings of the National Academy of Sciences USA, 107, 2361-2366.
116. Labarca P., Latorre R. (1992) Insertion of ion channels into planar lipid bilayers by vesicle fusion. Methods in Enzymology, 207, 447-463.
117. Laible P.D., Scott H.N., Henry L., Hanson D.K. (2004) Towards higher-throughput membrane protein production for structural genomics initiatives. Journal of Structural and Functional Genomics, 5, 167-172.
118. Lederman M., Zubay G. (1967) DNA-directed peptide synthesis. 1. A comparison of T2 and Escherichia coli DNA-directed peptide synthesis in two cell-free systems. Biochimica et Biophysica Acta, 149, 253-258.
119. Ligaba A., Katsuhara M., Ryan P.R., Shibasaka M., Matsumoto H. (2006) The BnALMT1 and BnALMT2 genes from rape encode aluminum-activated malate transporters that enhance the aluminum resistance of plant cells. Plant Physiology, 142, 1294-1303.
120. Liguori L., Blesneac I., Madern D., Vivaudou M., Lenormand J.-L. (2010) Single-step production of functional OEP24 proteoliposomes. Protein Expression and Purification, 69, 106-111.
121. Lingwood D., Simons K. (2010) Lipid rafts as a membrane-organizing principle. Science, 327, 46-50.
122. Linka N., Weber A.P.M. (2010) Intracellular metabolite transporters in plants. Molecular Plant, 3, 21-53.
123. Linka N., Theodoulou F.L., Haslam R.P., Linka M., Napier J.A., Neuhaus H.E., Weber A.P.M. (2008) Peroxisomal ATP import is essential for seedling development in Arabidopsis thaliana. Plant Cell, 20, 3241-3257.
124. Liu L.H., Ludewig L., Gassert B., Frommer W.B., von Wirén N. (2003) Urea transport by nitrogen-regulated tonoplast intrinsic proteins in Arabidopsis. Plant Physiology, 133, 1220-1228.
125. Liu J., Sitaram A., Burd C.G. (2007) Regulation of copper-dependent endocytosis and vacuolar degradation of the yeast copper transporter, Ctr1p, by the Rsp5 ubiquitin ligase. Traffic, 8, 1375-1384.
126. Loddenkötter B., Kammerer B., Fischer K., Flügge U.-I. (1993) Expression of the functional mature chloroplast triose phosphate translocator in yeast internal membranes and purification of the histidine-tagged protein by a single metal-affinity chromatography step. Proceedings of the National Academy of Sciences USA, 90, 2155-2159.
127. Looger L.L., Lalonde S., Frommer W.B. (2005) Genetically encoded FRET sensors for visualizing metabolites with subcellular resolution in living cells. Plant Physiology, 138, 555-557.
128. 3 transport into the vacuole. Plant Physiology, 137, 671-680.
129. Ludewig U., Frommer W.B. (2002) Genes and protein for solute transport and sensing. The Arabidopsis Book, 1, e0092.doi:.
130. 4 uniporters. Journal of Biological Chemistry, 278, 45603-45610.
131. 2+-ATPase from sarcoplasmic reticulum. Journal of Biological Chemistry, 264, 4907-4915.
132. Mahdieh M., Mostajeran A., Horie T., Katsuhara M. (2008) Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants. Plant and Cell Physiology, 49, 801-813.
133. Matsumori N., Murata M. (2010) 3D structures of membrane-associated small molecules as determined in isotropic bicelles. Natural Product Reports, 27, 1480-1492.
134. Matthaei H., Nirenberg M.W. (1961) The dependence of cell-free protein synthesis in E. coli upon RNA prepared from ribosomes. Biochemical and Biophysical Research Communications, 4, 404-408.
135. Maycox P.R., Deckwerth T., Jahn R. (1990) Bacteriorhodopsin drives the glutamate transporter of synaptic vesicles after co-reconstitution. EMBO Journal, 9, 1465-1469.
136. Miledi R., Parker I., Sumikawa K. (1982) Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes. EMBO Journal, 1, 1307-1312.
137. Miller A.J., Zhou J.J. (2000) Xenopus oocytes as an expression system for plant transporters. Biochimica et Biophysica Acta, 1465, 343-358.
138. Minet M., Dufour M.E., Lacroute F. (1992) Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant Journal, 2, 417-422.
139. Miroux B., Walker J.E. (1996) Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. Journal of Molecular Biology, 260, 289-298.
140. Mitsukawa N., Okumura S., Shirano Y., Sato S., Kato T., Harashima S., Shibata D. (1997) Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. Proceedings of the National Academy of Sciences USA, 94, 7098-7102.
141. Möhlmann T., Tjaden J., Schwöppe C., Winkler H.H., Kampfenkel K., Neuhaus H.E. (1998) Occurrence of two plastidic ATP/ADP transporters in Arabidopsis thaliana L. - molecular characterisation and comparative structural analysis of similar ATP/ADP translocators from plastids and Rickettsia prowazekii. European Journal of Biochemistry, 252, 353-359.
142. Möller S., Croning M.D., Apweiler R. (2001) Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics, 17, 646-653.
143. Moran N., Ehrenstein G., Iwasa K., Bare C., Mischke C. (1984) Ion channels in plasmalemma of wheat protoplasts. Science, 226, 835-838.
144. Mueller P., Rudin D.O., Tien H.T., Wescott W.C. (1962) Reconstitution of cell membrane structure in vitro and its transformation into an excitable system. Nature, 194, 979-980.
145. Narimatsu Y., Kubota T., Furukawa S., Shimojima M., Iwasaki H., Tozawa Y., Tachibana K., Narimatsu H. (2011) Co-translational function of Cosmc, core 1 synthase specific molecular chaperone, revealed by a cell-free translation system. FEBS Letters, 585, 1276-1280.
146. Neher E., Sakmann B. (1976) Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260, 799-802.
147. Neuhaus H.E., Thom E., Möhlmann T., Steup M., Kampfenkel K. (1997) Characterization of a novel eukaryotic ATP/ADP translocator located in the plastid envelope of Arabidopsis thaliana L. Plant Journal, 11, 73-82.
148. Newstead S., Kim H., von Heijne G., Iwata S., Drew D. (2007) High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences USA, 104, 13936-13941.
149. Niewiadomski P., Knappe S., Geimer S., Fischer K., Schulz B., Unte U.S., Rosso M.G., Ache P., Flügge U.I., Schneider A. (2005) The Arabidopsis plastidic glucose 6-phosphate/phosphate translocator GPT1 is essential for pollen maturation and embryo sac development. Plant Cell, 17, 760-775.
150. Niu L., Kim J.M., Khorana H.G. (2002) Structure and function in rhodopsin: asymmetric reconstitution of rhodopsin in liposomes. Proceedings of the National Academy of Sciences USA, 99, 13409-13412.
151. Norambuena L., Marchant L., Berninsone P., Hirschberg C.B., Silva H., Orellana A. (2002) Transport of UDP-galactose in plants. Identification and functional characterization of AtUTr1, an Arabidopsis thaliana UDP-galactose/UDP-glucose transporter. Journal of Biological Chemistry, 277, 32923-32929.
152. Nozawa A., Nanamiya H., Miyata T., Linka N., Endo Y., Weber A.P.M., Tozawa Y. (2007) A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters. Plant Cell Physiology, 48, 1815-1820.
153. Nozawa A., Ogasawara T., Matsunaga S., Iwasaki T., Sawasaki T., Endo Y. (2011) Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system. BMC Biotechnology, 11, 35-44.
154. Okumoto S., Jones A., Frommer W.B. (2012) Quantitative imaging with fluorescent biosensors: advanced tools for spatiotemporal analysis of biodynamics in cells. Annual Review of Plant Biology 63, 1-26.
155. Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F., Miller A.J. (2006) Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction. Plant Physiology, 142, 1304-1317.
156. Palmieri L., Arrigoni R., Blanco E., Carrari F., Zanor M.I., Studart-Guimaraes C., Fernie A.R., Palmieri F. (2006a) Molecular identification of an Arabidopsis S-adenosylmethionine transporter. Analysis of organ distribution, bacterial expression, reconstitution into liposomes, and functional characterization. Plant Physiology, 142, 855-865.
157. Palmieri L., Todd C.D., Arrigoni R., Hoyos M.E., Santoro A., Polacco J.C., Palmieri F. (2006b) Arabidopsis mitochondria have two basic amino acid transporters with partially overlapping specificities and differential expression in seedling development. Biochimica et Biophysica Acta, 1757, 1277-1283.
158. Palmieri L., Picault N., Arrigoni R., Besin E., Palmieri F., Hodges M. (2008) Molecular identification of three Arabidopsis thaliana mitochondrial dicarboxylate carrier isoforms: organ distribution, bacterial expression, reconstitution into liposomes and functional characterization. Biochemical Journal, 410, 621-629.
159. Palmieri F., Pierri C.L., De G.A., Nunes-Nesi A., Fernie A.R. (2011) Evolution, structure and function of mitochondrial carriers: a review with new insights. Plant Journal, 66, 161-181.
160. Pandhal J., Wright P.C. (2010) N-Linked glycoengineering for human therapeutic proteins in bacteria. Biotechnology Letters, 32, 1189-1198.
161. Paternostre M.T., Roux M., Rigaud J.L. (1988) Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 1. Solubilization of large unilamellar liposomes (prepared by reverse-phase evaporation) by Triton X-100, octyl glucoside, and sodium cholate. Biochemistry, 27, 2668-2677.
162. +-dependent phosphate transporter: functional characterization in Escherichia coli. Journal of Biological Chemistry, 283, 13520-13527.
163. + channel expression represents an essential step in coleoptile growth and gravitropism. Proceedings of the National Academy of Sciences USA, 96, 12186-12191.
164. Picault N., Palmieri L., Pisano I., Hodges M., Palmieri F. (2002) Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution. Journal of Biological Chemistry, 277, 24204-24211.
165. Poschet G., Hannich B., Büttner M. (2010) Identification and characterization of AtSTP14, a novel galactose transporter from Arabidopsis. Plant and Cell Physiology, 51, 1571-1580.
166. Privé G.G. (2007) Detergents for the stabilization and crystallization of membrane proteins. Methods, 41, 388-397.
167. Qin X., Liu M., Yang D., Zhang X. (2010) Concentration-dependent aggregation of CHAPS investigated by NMR spectroscopy. Journal of Physical Chemistry, 114, 3863-3868.
168. + transporters from Arabidopsis that are conserved across phyla. FEBS Letters, 415, 206-211.
169. Ramsperger-Gleixner M., Geiger D., Hedrich R., Sauer N. (2004) Differential expression of sucrose transporter and polyol transporter genes during maturation of common plantain companion cells. Plant Physiology, 134, 147-160.
170. Ravaud S., Do Cao M.A., Jidenko M., Ebel C., Le Maire M., Jault J.M., Di Pietro A., Haser R., Aghajari N. (2006) The ABC transporter BmrA from Bacillus subtilis is a functional dimer when in a detergent-solubilized state. Biochemical Journal, 395, 345-353.
171. Reinders A., Sivitz A.B., Starker C.G., Gantt J.S., Ward J.M. (2008) Functional analysis of LjSUT4, a vacuolar sucrose transporter from Lotus japonicus. Plant Molecular Biology, 68, 289-299.
172. Reinsberg D., Booth P.J., Jegerschold C., Khoo B.J., Paulsen H. (2000) Folding, assembly, and stability of the major light-harvesting complex of higher plants, LHCII, in the presence of native lipids. Biochemistry, 39, 14305-14313.
173. Renne P., Dressen U., Hebbeker U., Hille D., Flügge U.I., Westhoff P., Weber A.P. (2003) The Arabidopsis mutant dct is deficient in the plastidic glutamate/malate translocator DiT2. Plant Journal, 35, 316-331.
174. Rigaud J.L., Lévy D. (2003) Reconstitution of membrane proteins into liposomes. Methods in Enzymology, 372, 65-86.
175. Rigaud J.L., Paternostre M.T., Bluzat A. (1988) Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 2. Incorporation of the light-driven proton pump bacteriorhodopsin. Biochemistry, 27, 2677-2688.
176. Rigaut G., Shevchenko A., Rutz B., Wilm M., Mann M., Séraphin B. (1999) A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnology, 17, 1030-1032.
177. Roche Y., Gerbeau-Pissot P., Buhot B., Thomas D., Bonneau L., Gresti J., Mongrand S., Perrier-Cornet J.M., Simon-Plas F. (2008) Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts. FASEB Journal, 22, 3980-3991.
178. Rollwitz I., Santaella M., Hille D., Flügge U.I., Fischer K. (2006) Characterization of AtNST-KT1, a novel UDP-galactose transporter from Arabidopsis thaliana. FEBS Letters, 580, 4246-4251.
179. Roosild T.P., Greenwald J., Vega M., Castronovo S., Riek R., Choe S. (2005) NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science, 307, 1317-1321.
180. Rubio F., Gassmann W., Schroeder J.I. (1995) Sodium-driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance. Science, 270, 1660-1663.
181. Sachs F., Auerbach A. (1983) Single-channel electrophysiology: use of the patch clamp. Methods in Enzymology, 103, 147-176.
182. Saier M.H., Tran C.V., Barabote R.D. (2006) TCDB: the Transporter Classification Database for membrane transport protein analyses and information. Nucleic Acids Research, 34, D181-D186.
183. Saier M.H., Yen M.R., Noto K., Tamang D.G., Elkan C. (2009) The Transporter Classification Database: recent advances. Nucleic Acids Research, 37, D274-D278.
184. Sakr S., Alves G., Morillon R., Maurel K., Decourteix M., Guilliot A., Fleurat-Lessard P., Julien J.L., Chrispeels M.J. (2003) Plasma membrane aquaporins are involved in winter embolism recovery in walnut tree. Plant Physiology, 133, 630-641.
185. Santa-María G.E., Rubio F., Dubcovsky J., Rodriguez-Navarro A. (1997) The HAK1 gene of barley is a member of a large gene family and encodes a high-affinity potassium transporter. Plant Cell, 9, 2281-2289.
186. Sasaki Y., Asayama W., Niwa T., Sawada S., Ueda T., Taguchi H., Akiyoshi K. (2011) Amphiphilic polysaccharide nanogels as artificial chaperones in cell-free protein synthesis. Macromolecular Bioscience, 11, 814-820.
187. Sauer N., Stolz J. (1994) SUC1 and SUC2: two sucrose transporters from Arabidopsis thaliana; expression and characterization in baker's yeast and identification of the histidine-tagged protein. Plant Journal, 6, 67-77.
188. Sauer N., Caspari T., Klebl F., Tanner W. (1990a) Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences USA, 87, 7949-7952.
189. Sauer N., Friedländer K., Gräml-Wicke U. (1990b) Primary structure, genomic organization and heterologous expression of a glucose transporter from Arabidopsis thaliana. EMBO Journal, 9, 3045-3050.
190. Schachtman D.P., Schroeder J.I., Lucas W.J., Anderson J.A., Gaber R.F. (1992) Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science, 258, 1654-1658.
191. Schaefer D., Zryd J.P., Knight C.D., Cove D.J. (1991) Stable transformation of the moss Physcomitrella patens. Molecular and General Genetics, 226, 418-424.
192. Schneider S., Beyhl D., Hedrich R., Sauer N. (2008) Functional and physiological characterization of Arabidopsis INOSITOL TRANSPORTER1, a novel tonoplast-localized transporter for myo-inositol. Plant Cell, 20, 1073-1087.
193. Schroeder J.I., Hedrich R., Fernandez J.M. (1984) Potassium-selective single channels in guard cell protoplasts of Vicia faba. Nature, 312, 361-362.
194. Schwacke R., Schneider A., van der Graaff E., Fischer K., Catoni E., Desimone M., Frommer W.B., Flügge U.I., Kunze R. (2003) ARAMEMNON, a novel database for Arabidopsis integral membrane proteins. Plant Physiology, 131, 16-26.
195. Schwacke R., Flügge U.-I., Kunze R. (2004) Plant membrane proteome databases. Plant Physiology and Biochemistry, 42, 1023-1034.
196. Schwarz M., Gross A., Steinkamp T., Flügge U.I., Wagner R. (1994) Ion channel properties of the reconstituted chloroplast triose phosphate/phosphate translocator. Journal of Biological Chemistry, 269, 29481-29489.
197. Schwarz D., Daley D., Beckhaus T., Dötsch V., Bernhard F. (2010) Cell-free expression profiling of E. coli inner membrane proteins. Proteomics, 10, 1762-1779.
198. Schwöppe C., Winkler H.H., Neuhaus H.E. (2002) Properties of the glucose 6-phosphate transporter from Chlamydia pneumoniae (HPTcp) and the glucose 6-phosphate sensor from Escherichia coli (UhpC). Journal of Bacteriology, 184, 2108-2115.
199. Seddon A.M., Curnow P., Booth P.J. (2004) Membrane proteins, lipids and detergents: not just a soap opera. Biochimica et Biophysica Acta, 1666, 105-117.
200. 1-ATP synthase. Biochemical Journal, 346, 41-44.
201. Sigel E., Minier F. (2005) The Xenopus oocyte: system for the study of functional expression and modulation of proteins. Molecular Nutrition and Food Research, 49, 228-234.
202. Silvius J.R. (1992) Solubilization and functional reconstitution of biomembrane components. Annual Review of Biophysics and Biomolecular Structure, 21, 323-348.
203. Singh S.M., Panda A.K. (2005) Solubilization and refolding of bacterial inclusion body proteins. Journal of Biosciences and Bioengineering, 99, 303-310.
204. Smith G.E., Vlak J.M., Summers M.D. (1982) In vitro translation of Autographa californica nuclear polyhedrosis virus early and late mRNAs. Journal of Virology, 44, 199-208.
205. Sobczak K., Bangel-Ruland N., Leier G., Weber W.-M. (2010) Endogenous transport systems in the Xenopus laevis oocyte plasma membrane. Methods, 51, 183-189.
206. Spirin A.S. (2004) High-throughput cell-free systems for synthesis of functionally active proteins. Trends in Biotechnology, 22, 538-545.
207. Spirin A.S., Baranov V.I., Ryabova L.A., Ovodov S.Y., Alakhov Y.B. (1988) A continuous cell-free translation system capable of producing polypeptides in high yield. Science, 242, 1162-1164.
208. Steinkamp T., Hill K., Hinnah S.C., Wagner R., Rohl T., Pohlmeyer K., Soll J. (2000) Identification of the pore-forming region of the outer chloroplast envelope protein OEP16. Journal of Biological Chemistry, 275, 11758-11764.
209. + symporter in lipid vesicles and purification of the histidine tagged protein from transgenic Saccharomyces cerevisiae. Plant Journal, 6, 225-233.
210. Struhl K., Cameron J.R., Davis R.W. (1976) Functional genetic expression of eukaryotic DNA in Escherichia coli. Proceedings of the National Academy of Sciences USA, 73, 1471-1475.
211. Studier F.W., Moffatt B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology, 189, 113-130.
212. Takabatake R., Hata S., Taniguchi M., Kouchi H., Sugiyama T., Izui K. (1999) Isolation and characterization of cDNAs encoding mitochondrial phosphate transporters in soybean, maize, rice, and Arabidopsis. Plant Molecular Biology, 40, 479-486.
213. Taniguchi M., Taniguchi Y., Kawasaki M., Takeda S., Kato T., Tabata H., Miyake H., Sugiyama T. (2002) Identifying and characterizing plastidic 2-oxoglutarate/malate and dicarboxylate transporters in Arabidopsis thaliana. Plant Cell Physiology, 43, 706-717.
214. Terpe K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60, 523-533.
215. Terpe K. (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 72, 211-222.
216. Tjaden J., Schwöppe C., Möhlmann T., Quick P.W., Neuhaus H.E. (1998) Expression of a plastidic ATP/ADP transporter gene in Escherichia coli leads to a functional adenine nucleotide transport system in the bacterial cytoplasmic membrane. Journal of Biological Chemistry, 273, 9630-9636.
217. Törnroth-Horsefield S., Wang Y., Hedfalk K., Johanson U., Karlsson M., Tajkhorshid E., Neutze R., Kjellbom P. (2006) Structural mechanism of plant aquaporin gating. Nature, 439, 688-694.
218. Traub M., Flörchinger M., Piecuch J., Kunz H.-H., Weise-Steinmetz A., Deitmer J.W., Neuhaus H.E., Möhlmann T. (2007) The fluorouridine insensitive 1 (fur1) mutant is defective in equilibrative nucleoside transporter 3 (ENT3), and thus represents an important pyrimidine nucleoside uptake system in Arabidopsis thaliana. Plant Journal, 49, 855-864.
219. Trentmann O., Horn M., van Scheltinga A.C., Neuhaus H.E., Haferkamp I. (2007) Enlightening energy parasitism by analysis of an ATP/ADP transporter from Chlamydiae. PLos Biology, 5, e231.
220. Trentmann O., Jung B., Neuhaus H.E., Haferkamp I. (2008) Non-mitochondrial ATP/ADP transporters accept phosphate as third substrate. Journal of Biological Chemistry, 283, 36486-36493.
221. Tucker J., Grisshammer R. (1996) Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochemical Journal, 317, 891-899.
222. Turner G.J., Miercke L.J., Mitra A.K., Stroud R.M., Betlach M.C., Winter-Vann A. (1999) Expression, purification, and structural characterization of the bacteriorhodopsin-aspartyltranscarbamylase fusion protein. Protein Expression and Purification, 17, 324-338.
223. Vergis J.M., Wiener M.C. (2011) The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal. Protein Expression and Purification, 78, 139-142.
224. Versaw W.K., Harrison M.J. (2002) A chloroplast phosphate transporter, PHT2;1, influences allocation of phosphate within the plant and phosphate-starvation responses. Plant Cell, 14, 1751-1766.
225. Wacker M., Linton D., Hitchen P.G., Nita-Lazar M., Haslam S.M., North S.J., Panico M., Morris H.R., Dell A., Wren B.W., Aebi M. (2002) N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science, 298, 1790-1793.
226. Wagner S., Baars L., Ytterberg A.J., Klussmeier A., Wagner C.S., Nord O., Nygren P.A., van Wijk K.J., de Gier J.W. (2007) Consequences of membrane protein overexpression in Escherichia coli. Molecular and Cellular Proteomics, 6, 1527-1550.
227. Wagner S., Klepsch M.M., Schlegel S., Appel A., Draheim R., Tarry M., Högbom M., van Wijk K.J., Slotboom D.J., Persson J.O., de Gier J.W. (2008) Tuning Escherichia coli for membrane protein overexpression. Proceedings of the National Academy of Sciences USA, 105, 14371-14376.
228. Weber A., Menzlaff E., Arbinger B., Gutensohn M., Eckerskorn C., Flügge U.I. (1995) The 2-oxoglutarate/malate translocator of chloroplast envelope membranes: molecular cloning of a transporter containing a 12-helix motif and expression of the functional protein in yeast cells. Biochemistry, 34, 2621-2627.
229. Weber H., Borisjuk L., Heim U., Sauer N., Wobus U. (1997) A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds. Plant Cell, 9, 895-908.
230. Weise A., Altmann F., Rodriguez-Franco M., Sjoberg E., Baumer W., Launhardt H., Kietzmann M., Gorr G. (2007) High-level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella delta-fuc-t & delta-xyl-t mutant. Plant Biotechnology Journal, 5, 389-401.
231. White M.A., Clark K.M., Grayhack E.J., Dumont M.E. (2007) Characteristics affecting expression and solubilization of yeast membrane proteins. Journal of Molecular Biology, 365, 621-636.
232. Woodbury D.J., Hall J.E. (1988) Role of channels in the fusion of vesicles with a planar bilayer. Biophysical Journal, 54, 1053-1063.
233. Wormit A., Traub M., Flörchinger M., Neuhaus H.E., Möhlmann T. (2004) Characterization of three novel members of the Arabidopsis thaliana equilibrative nucleoside transporter (ENT) family. Biochemical Journal, 383, 19-26.
234. Wu Z., Zhao J., Gao R., Hu G., Gai J., Xu G., Xing H. (2011) Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max. PLoS ONE, 6, e19752.
235. + transporter AKT1 in Arabidopsis. Cell, 125, 1347-1360.
236. Yang H., Murphy A.S. (2009) Functional expression and characterization of Arabidopsis ABCB, AUX 1 and PIN auxin transporters in Schizosaccharomyces pombe. Plant Journal, 59, 179-191.
237. Zhang X.P., Glaser E. (2002) Interaction of plant mitochondrial and chloroplast signal peptides with the Hsp70 molecular chaperone. Trends in Plant Science, 7, 14-21.
238. 3+-activated anion channel in transformed tobacco (Nicotiana tabacum L.) cells. Plant Cell Physiology, 49, 1316-1330.
239. Zhang B., Liu X., Qian Q., Liu L., Dong G., Xiong G., Zeng D., Zhou Y. (2011) Golgi nucleotide sugar transporter modulates cell wall biosynthesis and plant growth in rice. Proceedings of the National Academy of Sciences USA, 108, 5110-5115.
240. Zuo X., Mattern M.R., Tan R., Li S., Hall J., Sterner D.E., Shoo J., Tran H., Lim P., Sarafianos S.G., Kazi L., Navas-Martin S., Weiss S.R., Butt T.R. (2005) Expression and purification of SARS coronavirus proteins using SUMO-fusions. Protein Expression and Purification, 42, 100-110.