NMR structure of mistic, a membrane-integrating protein for membrane protein expression

Science

Volumn 307, Issue 5713, 2005, Pages 1317-1321

  Roosild, Tarmo P ^a   Greenwald, Jason ^a   Vega, Mark ^a   Castronovo, Samantha ^a   Riek, Roland ^a   Choe, Senyon ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOLOGICAL MEMBRANES; CONFORMATIONS; CRYSTALS; LIPIDS; NUCLEAR MAGNETIC RESONANCE; PROBES;

BACILLUS SUBTILIS; MEMBRANE PROTEINS; MISTIC;

PROTEINS;

BACTERIAL PROTEIN; INTEGRAL MEMBRANE PROTEIN; MEMBRANE PROTEIN; MISTIC PROTEIN; UNCLASSIFIED DRUG;

BIOLOGY;

ARTICLE; BACILLUS SUBTILIS; CRYSTALLIZATION; ESCHERICHIA COLI; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CELL MEMBRANE; CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; HYDROGEN BONDING; LIPID BILAYERS; MEMBRANE PROTEINS; MICELLES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; RECOMBINANT PROTEINS;

EID: 14544292475     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1106392     Document Type: Article

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28. We thank E. Wiater for help in TGF-β receptor binding studies and C. Park for Edman degradation sequencing of proteins. Supported by NIH grant GM056653. R.R. is a Pew scholar. The bundle of 10 conformers representing the NMR structure is deposited in the PDB database with accession code 1YGM. The coding sequence of Mistic has been deposited in GenBank with accession code AY874162.