Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Nozawa, Akira ^a,b   Ogasawara, Tomio ^a,b   Matsunaga, Satoko ^a   Iwasaki, Takahiro ^a   Sawasaki, Tatsuya ^a,b   Endo, Yaeta ^a,b  

^a EHIME UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASSOCIATION RATES; CELL FREE PROTEIN SYNTHESIS; CELL-FREE; CELL-FREE SYSTEM; CELL-FREE TRANSLATION SYSTEMS; COMPLEX FORMATIONS; COOMASSIE BRILLIANT BLUE; DENSITY GRADIENTS; FUNCTIONAL MEMBRANES; GREEN FLUORESCENT PROTEIN; LARGE SCALE PREPARATION; MEAN VALUES; MEMBRANE PROTEINS; OPTIMAL CONDITIONS; PARTIAL PURIFICATION; PHOSPHATE TRANSPORTERS; SOLUBLE PROTEINS; SYNTHESIS REACTION; TRANS-MEMBRANE DOMAINS; TRANSPORT ACTIVITY; ULTRACENTRIFUGATION;

ACTIVATION ANALYSIS; BIOLOGICAL MEMBRANES; CELLS; CENTRIFUGATION; LIPOSOMES; MAMMALS; OPTIMIZATION; PHOSPHOLIPIDS; PURIFICATION; SYNTHESIS (CHEMICAL);

PROTEINS;

ANAZOLENE SODIUM; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LIPOSOME; MEMBRANE PROTEIN; PHOSPHATE TRANSPORTER; PURINERGIC RECEPTOR; ARABIDOPSIS PROTEIN; CARRIER PROTEIN; PHOSPHATE TRANSLOCATOR 1, ARABIDOPSIS;

ARTICLE; CELL FREE SYSTEM; COMPLEX FORMATION; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; HUMAN; LARGE SCALE PRODUCTION; MAMMAL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; WHEAT; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; SPECTROFLUOROMETRY; TIME; ULTRACENTRIFUGATION;

MAMMALIA; TRITICUM AESTIVUM;

ARABIDOPSIS PROTEINS; CELL-FREE SYSTEM; CENTRIFUGATION, DENSITY GRADIENT; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GREEN FLUORESCENT PROTEINS; LIPOSOMES; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TIME FACTORS; TRITICUM; ULTRACENTRIFUGATION;

EID: 79953802067     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-35     Document Type: Article

References (32)

1. International Human Genome Sequencing Consortium Initial sequencing and analysis of the human genome. Nature 2001, 409:860-921. 10.1038/35057062, 11237011, International Human Genome Sequencing Consortium.
2. Arabidopsis Genome Initiative Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 2000, 408:796-815. 10.1038/35048692, 11130711, Arabidopsis Genome Initiative.
3. Wagner S, Bader ML, Drew D, de Gier JW. Rationalizing membrane protein overexpression. Trends Biotechnol 2006, 24:364-371. 10.1016/j.tibtech.2006.06.008, 16820235.
4. Ishihara G, Goto M, Saeki M, Ito K, Hori T, Kigawa T, Shirouzu M, Yokoyama S. Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors. Protein Expr Purif 2005, 41:27-37. 10.1016/j.pep.2005.01.013, 15802218.
5. Liguori L, Marques B, Villegas-Méndez A, Rothe R, Lenormand JL. Production of membrane proteins using cell-free expression systems. Expert Rev Proteomics 2007, 4:79-90. 10.1586/14789450.4.1.79, 17288517.
6. Schwarz D, Dötsch V, Bernhard F. Production of membrane proteins using cell-free expression systems. Proteomics 2008, 8:3933-3946. 10.1002/pmic.200800171, 18763710.
7. Berrier C, Park KH, Abes S, Bibonne A, Betton JM, Ghazi A. Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. Biochemistry 2004, 43:12585-12591. 10.1021/bi049049y, 15449948.
8. Liguori L, Marques B, Villegas-Méndez A, Rothe R, Lenormand JL. Liposome-mediated delivery of pro-apoptotic therapeutic membrane proteins. J Cont Rel 2008, 126:217-227.
9. Katzen F, Peterson TC, Kudlicki W. Membrane protein expression: no cells required. Trends Biotechnol 2009, 27:455-460. 10.1016/j.tibtech.2009.05.005, 19616329.
10. Shimono K, Goto M, Kikukawa T, Miyauchi S, Shirouzu M, Kamo N, Yokoyama S. Production of functional bacteriorhodopsin by an Escherichia coli cell-free protein synthesis system supplemented with steroid detergent and lipid. Protein Sci 2009, 18:2160-2171. 10.1002/pro.230, 2786979, 19746358.
11. Lim L, Linka M, Mullin KA, Weber AP, McFadden GI. The carbon and energy sources of the non-photosynthetic plastid in the malaria parasite. FEBS Lett 2010, 584:549-554. 10.1016/j.febslet.2009.11.097, 19968990.
12. Klammt C, Schwarz D, Eifer N, Engel A, Piehler J, Haase W, Hahn S, Dötsch V, Bernhard F. Cell-free production of G protein-coupled receptors for functional and structural studies. J Struct Biol 2007, 158:482-493. 10.1016/j.jsb.2007.01.006, 17350285.
13. Kaiser L, Graveland-Bikker J, Steuerwald D, Vanberghem M, Herlihy K, Zhang S. Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses. Proc Natl Acad Sci USA 2008, 105:15726-15731. 10.1073/pnas.0804766105, 2572932, 18840687.
14. Kalmbach R, Chizhov I, Schumacher MC, Friedrich T, Bamberg E, Engelhard M. Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomes. J Mol Biol 2007, 371:639-648. 10.1016/j.jmb.2007.05.087, 17586523.
15. Goren MA, Fox BG. Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. Protein Expr Purif 2008, 62:171-178. 10.1016/j.pep.2008.08.002, 2586813, 18765284.
16. Nozawa A, Nanamiya H, Miyata T, Linka N, Endo Y, Weber AP, Tozawa Y. A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters. Plant Cell Physiol 2007, 48:1815-1820. 10.1093/pcp/pcm150, 17981875.
17. Klabunde T, Hessler G. Drug design strategies for targeting G-protein-coupled receptors. Chembiochem 2002, 3:928-944. 10.1002/1439-7633(20021004)3:10<928::AID-CBIC928>3.0.CO;2-5, 12362358.
18. Sawasaki T, Ogasawara T, Morishita R, Endo Y. A cell-free protein synthesis system for high-throughput proteomics. Proc Natl Acad Sci USA 2002, 99:14652-14657. 10.1073/pnas.232580399, 137474, 12409616.
19. Sawasaki T, Gouda MD, Kawasaki T, Tsuboi T, Tozawa Y, Takai K, Endo Y. The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information. Methods Mol Biol 2005, 310:131-144. full_text, 16350952.
20. Katzen F, Fletcher JE, Yang JP, Kang D, Peterson TC, Cappuccio JA, Blanchette CD, Sulchek T, Chromy BA, Hoeprich PD, Coleman MA, Kudlicki W. Insertion of membrane proteins into discoidal membranes using a cell-free protein expression approach. J Prot Res 2008, 7:3535-3542.
21. Moritani Y, Nomura SM, Morita I, Akiyoshi K. Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation. FEBS J 2010, 277:3343-3352.
22. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Cell-free translation reconstituted with purified components. Nat Biotechnol 19:751-755. 10.1038/90802, 11479568.
23. Kaneda M, Nomura SM, Ichinose S, Kondo S, Nakahama K, Akiyoshi K, Morita I. Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes. Biomaterials 2009, 30:3971-3977. 10.1016/j.biomaterials.2009.04.006, 19423159.
24. Sobrado P, Goren MA, James D, Amundson CK, Fox BG. A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles. Protein Expr Purif 2008, 58:229-241. 10.1016/j.pep.2007.11.018, 2277500, 18226920.
25. Graham JM, Ford T, Rickwood D. Isolation of the major subcellular organelles from liver using Nycodenz gradients without the use of an ultracentrifuge. Anal Biochem 1990, 187:318-323. 10.1016/0003-2697(90)90463-J, 2382834.
26. Sbracia M, Sayme N, Grasso J, Vigue L, Huszar G. Sperm function and choice of preparation media: comparison of Percoll and Accudenz discontinuous density gradients. J Androl 1996, 17:61-67.
27. Nomura SM, Kondoh S, Asayama W, Asada A, Nishikawa S, Akiyoshi K. Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis. J Biotechnol 2008, 133:190-195. 10.1016/j.jbiotec.2007.08.023, 17900734.
28. Madin K, Sawasaki T, Ogasawara T, Endo Y. A highly efficient and robustcell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. Proc Natl Acad Sci USA 2000, 97:559-564. 10.1073/pnas.97.2.559, 15369, 10639118.
29. Takai K, Sawasaki T, Endo Y. Practical cell-free protein synthesis system using purified wheat embryos. Nature Protoc 2010, 5:227-238.
30. Goren MA, Nozawa A, Makino S, Wrobel RL, Fox BG. Cell-free translation of integral membrane proteins into unilamelar liposomes. Methods Enzymol 2009, 463:647-673. full_text, 19892197.
31. Sawasaki T, Hasegawa Y, Tsuchimochi M, Kamura N, Ogasawara T, Kuroita T, Endo Y. A bilayer cell-free protein synthesis system for high-throughput screening of gene products. FEBS Lett 2002, 514:102-105. 10.1016/S0014-5793(02)02329-3, 11904190.
32. Nozawa A, Nanamiya H, Tozawa Y. Production of membrane proteins through the wheat-germ cell-free technology. Methods Mol Biol 2010, 607:213-218. full_text, 20204859.