Towards higher-throughput membrane protein production for structural genomics initiatives

Journal of Structural and Functional Genomics

Volumn 5, Issue 1-2, 2004, Pages 167-172

  Laible, Philip D ^a   Scott, Heather N ^a   Henry, Lynda ^b   Hanson, Deborah K ^a  

^a ARGONNE NATIONAL LABORATORY   (United States)

^b Amersham Biosciences   (United States)

Author keywords

Detergent solubilization; FPLC ; Heterologous expression; Immobilized metal affinity chromatography (IMAC); Ion exchange chromatography; Membrane proteins; Rhodobacter; Semi automated protein purification; Size exclusion chromatography

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CRYSTALLIZATION; FAST PROTEIN LIQUID CHROMATOGRAPHY; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; GENOMICS; HIGH THROUGHPUT SCREENING; ION EXCHANGE CHROMATOGRAPHY; LABORATORY AUTOMATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PHOTOTROPHIC BACTERIUM; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN INDUCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RHODOBACTER; X RAY DIFFRACTION;

CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MEMBRANE PROTEINS; MOLECULAR STRUCTURE; PROTEOMICS; RECOMBINANT PROTEINS; RHODOBACTER SPHAEROIDES;

BACTERIA (MICROORGANISMS); PHOTOBACTERIA; RHODOBACTER;

EID: 3543074650     PISSN: 1345711X     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:JSFG.0000029201.33710.46     Document Type: Article

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