A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters

Plant and Cell Physiology

Volumn 48, Issue 12, 2007, Pages 1815-1820

  Nozawa, Akira ^a   Nanamiya, Hideaki ^a   Miyata, Takuji ^a   Linka, Nicole ^b   Endo, Yaeta ^a   Weber, Andreas P M ^b   Tozawa, Yuzuru ^a  

^a EHIME UNIVERSITY   (Japan)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Cell free translation system; Phosphoenolpyruvate; Proteoliposome; Transporter; Wheat germ

Indexed keywords

CARRIER PROTEIN; PRIMER DNA; PROTEOLIPID; PROTEOLIPOSOMES; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; CELL FREE SYSTEM; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROTEIN SYNTHESIS;

ARABIDOPSIS; BASE SEQUENCE; CELL-FREE SYSTEM; DNA PRIMERS; MEMBRANE TRANSPORT PROTEINS; PROTEIN BIOSYNTHESIS; PROTEOLIPIDS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; TRITICUM AESTIVUM;

EID: 37149014145     PISSN: 00320781     EISSN: 14719053     Source Type: Journal    
DOI: 10.1093/pcp/pcm150     Document Type: Article

References (18)

1. Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-815.
2. Elbaz, Y., Steiner-Mordoch, S., Danieli, T. and Schuldiner, S. (2004) In vitro synthesis of fully functional EmeE, a multidrug transporter, and study of its oligomeric state. Proc. Natl Acad. Sci. USA 101: 1519-1524.
3. Fischer, K., Kammerer, B., Gutensohn, M., Arbinger, B., Weber, A., Häusler, R.E. and Flügge, U. (1997) A new class of plastidic phosphate translocators: a putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter. Plant Cell 9: 453-462.
4. Flügge, U. and Weber, A. (1994) A rapid method for measuring organelle-specific substrate transport in homogenates from plant tissues. Planta 194: 181-185.
5. Flügge, U.I., Weber, A., Fischer, K., Lottspeich, F., Eckerskorn, C., Waegemann, K. and Soll, J. (1991) The major chloroplast envelope polypeptide is the phosphate translocator and not the protein import receptor. Nature 353: 364-367.
6. Ishihara, G., Goto, M., Saeki, M., Ito, K., Hori, T., Kigawa, T., Shirouzu, M. and Yokoyama, S. (2005) Expression of G protein coupled receptors in a cell-free translational system using detergent and thioredoxin-fusion vectors. Protein Expr. Purif. 41: 27-37.
7. Kammerer, B., Fischer, K., Hilpert, B., Schubert, S., Gutensohn, M., Weber, A. and Flügge, U. (1998) Molecular characterization of a carbon transporter in plastids from heterotrophic tissues: the glucose 6-phosphate/phosphate antiporter. Plant Cell 10: 105-117.
8. Kasahara, M. and Hinkle, P.C. (1976) Reconstitution of D-glucose transport catalyzed by a protein fraction from human erythrocytes in sonicated liposomes. Proc. Natl Acad. Sci. USA 73: 396-400.
9. Kasai, K., Kawagishi-Kobayashi, M., Teraishi, M., Ito, Y., Ochi, K., Wakasa, K. and Tozawa, Y. (2004) Differential expression of three plastidial sigma factors, OsSIG1, OsSIG2A, and OsSIG2B, during leaf development in rice. Biosci. Biotechnol. Biochem. 68: 973-977.
10. Katzen, F., Chang, G. and Kudlicki, W. (2005) The past, present and future of cell-free protein synthesis. Trends Biotechnol. 23: 150-156.
11. Klammt, C., Schwarz, D., Fendler, K., Haase, W., Hahn, S., Dötsch, V. and Bernhard, F. (2005) Evaluation of detergents for the soluble expression of α-helical and β-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J. 272: 6024-6038.
12. Klammt, C., Schwarz, D., Löhr, F., Schneider, B., Dötsch, V. and Bernhard, F. (2006) Cell-free expression as an emerging technique for the large scale production of integral membrane protein. FEBS J. 273: 4141-4153.
13. Knappe, S., Löttgert, T., Schneider, A., Voll, L., Flügge, U. and Fischer, K. (2003) Characterization of two functional phosphoenolpyruvate/phosphate translocator (PPT) genes in Arabidopsis - AtPPT1 may be involved in the provision of signals for correct mesophyll development. Plant J. 36: 411-420.
14. Loddenkötter, B., Kammerer, B., Fischer, K. and Flügge, U. (1993) Expression of the functional mature chloroplast triose phosphate translocator in yeast internal membranes and purification of the histidine-tagged protein by a single metal-affinity chromatography step. Proc. Natl Acad. Sci. USA 90: 2155-2159.
15. Sawasaki, T., Hasegawa, Y., Tsuchimochi, M., Kamura, N., Ogasawara, T., Kuroita, T. and Endo, Y. (2002b) A bilayer cell-free synthesis system for high-throughput screening of gene products. FEBS Lett. 514: 102-105.
16. Sawasaki, T., Ogasawara, T., Morishita, R. and Endo, Y. (2002a) A cell-free protein synthesis system for high-throughput proteomics. Proc. Natl Acad. Sci. USA 99: 14652-14657.
17. Streatfield, S.J., Weber, A., Kinsman, E.A., Häusler, R.E., Li, J., Post-Beittenmiller, D., Kaiser, W.M., Pyke, K.A., Flügge, U.-I. and Chory, J. (1999) The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development, and plastid-dependent nuclear gene expression. Plant Cell 11: 1609-1621.
18. Toyota, K., Tamura, M., Ohdan, T. and Nakamura, Y. (2006) Expression profiling of starch metabolism-related plastid translocator genes in rice. Planta 223: 248-257.