The fluorouridine insensitive 1 (fur1) mutant is defective in equilibrative nucleoside transporter 3 (ENT3), and thus represents an important pyrimidine nucleoside uptake system in Arabidopsis thaliana

Plant Journal

Volumn 49, Issue 5, 2007, Pages 855-864

  Traub, Michaela ^a   Flörchinger, Martin ^a   Piecuch, Jennifer ^a   Kunz, Hans Henning ^a   Weise Steinmetz, Alexandra ^a   Deitmer, Joachim W ^a   Neuhaus, H Ekkehard ^a   Möhlmann, Torsten ^a  

^a Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

Arabidopsis thaliana; Equilibrative nucleoside transporter; Expression analysis; Knock out mutant; Salvage pathway; Xenopus oocytes

Indexed keywords

ARABIDOPSIS THALIANA; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER; EXPRESSION ANALYSIS; KNOCK-OUT MUTANT; SALVAGE PATHWAY; XENOPUS OOCYTES;

AMINO ACIDS; CONCENTRATION (PROCESS); DNA; GROWTH KINETICS; PLANTS (BOTANY);

MUTAGENESIS;

5-FLUOROURIDINE; DRUG DERIVATIVE; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER; FLUOROURIDINE; UNCLASSIFIED DRUG; URIDINE;

ANIMAL; ARABIDOPSIS; ARTICLE; ELECTROPHYSIOLOGY; FEMALE; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; OOCYTE; SITE DIRECTED MUTAGENESIS; TRANSPORT AT THE CELLULAR LEVEL; XENOPUS;

ANIMALS; ARABIDOPSIS; BIOLOGICAL TRANSPORT; ELECTROPHYSIOLOGY; EQUILIBRATIVE NUCLEOSIDE TRANSPORT PROTEINS; FEMALE; GENE EXPRESSION REGULATION, PLANT; MUTAGENESIS, SITE-DIRECTED; OOCYTES; URIDINE; XENOPUS;

CELLS; MUTAGENS; NUCLEIC ACIDS; PROTEINS;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 33847128735     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2006.02998.x     Document Type: Article

References (30)

1. Acimovic, Y. and Coe, I.R. (2002) Molecular evolution of the equilibrative nucleoside transporter family: identification of novel family members in prokaryotes and eukaryotes. Mol. Biol. Evol. 19, 2199-2210.
2. Arai, M., Mitsuke, H., Ikeda, M., Xia, J., Kikuchi, T., Satake, M. and Shimizu, T. (2004) ConPred II: a consensus prediction method for obtaining transmembrane topology models with high reliability. Nucleic Acids Res. 32, 390-393.
3. + buffering mediated by sodium bicarbonate cotransport expressed in Xenopus oocytes. J. Biol. Chem. 279, 28057-28062.
4. Cabrita, M., Baldwin, S., Young, J. and Cass, C. (2002) Molecular biology and regulation of nucleoside and nucleobase transporter proteins in eukaryotes and prokaryotes. Biochem. Cell Biol. 80, 623-638.
5. Curie, C., Liboz, T., Bardet, C., Gander, E., Medale, C., Axelos, M. and Lescure, B. (1991) Cis and trans-acting elements involved in the activation of Arabidopsis thaliana A1 gene encoding the translation elongation factor EF-1 alpha. Nucleic Acids Res. 19, 1305-1310.
6. Fsihi, H., Kottwitz, B. and Bremer, E. (1993) Single amino acid substitutions affecting the substrate specificity of the Escherichia coli K-12 nucleoside-specific Tsx channel. J. Biol. Chem. 268, 17495-17503.
7. Gibeaut, D.M., Hulett, J., Cramer, G.R. and Seemann, J.R. (1997) Maximal biomass of Arabidopsis thaliana using a simple, low-maintenance hydroponic method and favorable environmental conditions. Plant Physiol. 115, 317-319.
8. Hakala, M.T. and Rustrum, Y.M. (1979) The potential of in vitro screening. In Cancer Drug Development (DeVita, V.T. and Busch, H., eds). New York: Academic Press, pp. 271-290.
9. Hirose, N., Makita, N., Yamaya, T. and Sakakibara, H. (2005) Functional characterization and expression analysis of a gene, OsENT2, encoding an equilibrative nucleoside transporter in rice suggest a function in cytokinine transport. Plant Physiol. 138, 196-206.
10. Hyde, R.J., Cass, C.E., Young, J.D. and Baldwin, S.A. (2001) The ENT family of eukaryote nucleoside and nucleobase transporters: recent advances in the investigation of structure/function relationships and the identification of novel isoforms. Mol. Membr. Biol. 18, 53-63.
11. Ito, H., Fukuda, J., Murata, K. and Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168.
12. Leroch, M., Kirchberger, S., Haferkamp, I., Wahl, M., Neuhaus, H.E. and Tjaden, J. (2005) Identification and characterization of a novel plastidic adenine nucleotide uniporter from Solanum tuberosum. J. Biol. Chem. 280, 17992-18000.
13. Li, G., Liu, K., Baldwin, S.A. and Wang, D. (2003) Equilibrative nucleoside transporters of Arabidopsis thaliana: cDNA cloning, expression pattern and analysis of transport activities. J. Biol. Chem. 278, 35732-35742.
14. + channel determined by construction of multimeric cDNAs. Neuron, 9, 861-871.
15. Moffatt, B.A. and Ashihara, H. (2002) Purine and pyrimidine nucleotide synthesis and metabolism. In The Arabidopsis Book (Somerville, C.R. and Meyerowitz, E.M., eds). American Society of Plant Biologists, Rockville doi/10.1199/tab.0018, http://www.aspb.org/publications/arabidopsis, pp. 1-20.
16. Möhlmann, T., Mezher, Z., Schwerdtfeger, G. and Neuhaus, H.E. (2001) Characterisation of a concentrative type of adenosine transporter from Arabidopsis thaliana (ENT1, At). FEBS Lett. 509, 370-374.
17. Murashige, F. and Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Plant Physiol. 15, 473-496.
18. Phillips, D.A., Joseph, C.M. and Hirsch, P.R. (1997) Occurrence of flavonoids and nucleosides in agricultural soils. Appl. Environ. Microbiol. 63, 4573-4577.
19. Reiser, J., Linka, N., Lemke, L., Jeblick, W. and Neuhaus, H.E. (2004) Molecular physiological analysis of the two plastidic ATP/ADP transporters from Arabidopsis. Plant Physiol. 36, 3524-3536.
20. Schmidt, A., Su, Y.H., Kunze, R., Warner, S., Hewitt, M., Slocum, R.D., Ludewig, U., Frommer, W.B. and Desimone, M. (2004) UPS1 and UPS2 from Arabidopsis mediate high affinity transport of uracil and 5-fluorouracil. J. Biol. Chem. 279, 44817-44824.
21. Sherson, S.M., Hemmann, G., Wallace, G., Forbes, S., Germain, V., Stadler, R., Bechtold, N., Sauer, N. and Smith, S.M. (2000) Monosaccharide/proton symporter AtSTP1 plays a major role in uptake and response of Arabidopsis seeds and seedlings to sugars. Plant J. 24, 849-857.
22. Stasolla, C., Katahira, R., Thorpe, T.A. and Ashihara, H. (2003) Purine and pyrimidine nucleotide metabolism in higher plants. J. Plant. Physiol. 160, 1271-1295.
23. Stein, A., Vaseduvan, G., Carter, N.S., Ullman, B., Landfear, S.M. and Kavanaugh, M.P. (2003) Equilibrative nucleoside transporter family members from Leishmania donovani are electrogenic proton symporters. J. Biol. Chem. 278, 35127-35134.
24. Sun, J., Hirose, N., Wang, X., Wen, P., Xue, L., Sakakibara, H. and Zuo, J. (2005) Arabidopsis SOI33/AtENT8 gene encodes a putative equilibrative nucleoside transporter that is involved in cytokinin transport in planta. J. Integr. Plant Biol. 47, 588-603.
25. Wagner, C.A., Friedrich, B., Setiawan, I., Lang, F. and Broer, S. (2000) The use of Xenopus laevis oocytes for the functional characterization of heterologously expressed membrane proteins. Cell Physiol. Biochem. 10, 1-12.
26. Weigel, D. and Glazebrook, J. (2002) Arabidopsis. A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
27. Wormit, A., Traub, M., Flörchinger, M., Neuhaus, H.E. and Möhlmann, T. (2004) Characterization of three novel members of the Arabidopsis thaliana equilibrative nucleoside transporter (ENT) family. Biochem. J. 383, 19-26.
28. Wu, K. and King, J. (1994) Biochemical and genetic characterization of 5-fluoro-2′-deoxyuridine-resistant mutants of Arabidopsis thaliana. Planta, 194, 117-122.
29. Ye, J. and van den Berg, B. (2005) Crystal structure of the bacterial nucleoside transporter Tsx. EMBO J. 23, 3187-3195.
30. Zimmermann, P., Hirsch-Hoffmann, M., Henning, L. and Gruissem, W. (2004) Genevestigator. Arabidopsis microarray database and analysis toolbox. Plant Physiol. 136, 2621-2632.