The Xenopus oocyte: System for the study of functional expression and modulation of proteins

Molecular Nutrition and Food Research

Volumn 49, Issue 3, 2005, Pages 228-234

  Sigel, Erwin ^a,b   Minier, Frédéric ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b Department of Pharmacology ^*  (Switzerland)

Author keywords

cRNA; mRNA; Protein expression; Protein function; Xenopus oocyte

Indexed keywords

PROTEIN;

CELL FUNCTION; INFORMATION SYSTEM; MEMBRANE POTENTIAL; MICROINJECTION; NONHUMAN; NUTRITION; OOCYTE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; REVIEW; XENOPUS;

ANIMALS; FEMALE; GENE EXPRESSION; HUMANS; MUTATION; OOCYTES; PROTEINS; RNA, COMPLEMENTARY; RNA, MESSENGER; STRUCTURE-ACTIVITY RELATIONSHIP; TRANSFECTION; XENOPUS LAEVIS;

ANURA; XENOPUS (GENUS); XENOPUS LAEVIS;

EID: 15944402496     PISSN: 16134125     EISSN: None     Source Type: Journal    
DOI: 10.1002/mnfr.200400104     Document Type: Review

References (29)

1. Sigel, E., Use of Xenopus oocytes for the functional expression of plasma membrane proteins. J. Membr. Biol. 1990, 117, 201-221.
2. Wagner, C. A., Friedrich, B., Setiawan, I., Lang, F., Broer, S., The use of Xenopus laevis oocytes for the functional characterization of heterologously expressed membrane proteins. Cell. Physiol. Biochem. 2000, 10, 1-12.
3. Dascal, N., The use of Xenopus oocytes for the study of ion channels. CRC Crit. Rev. Biochem. 1987, 22, 317-387.
4. Gurdon, J. B., Lane, C. D., Woodland, H. R., Marbaix, G., Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature 1971, 233, 177-182.
5. Mertz, J. E., Gurdon, J. B., Purified DNAs are transcribed after microinjection into Xenopus oocytes. Proc. Natl. Acad. Sci. USA 1977, 74, 1502-1506.
6. Sumikawa, K., Houghton, M., Emtage, J. S., Richards, B. M., Barnard, E. A., Active multi-subunit ACh receptor assembled by translation of heterologous mRNA in Xenopus oocytes. Nature 1981, 292, 862-864.
7. Barnard, E. A., Miledi, R., Sumikawa, K., Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes. Proc. R. Soc. Lond. B 1982, 215, 241-246.
8. Rungger, D., Xenopus helveticus, an endangered species? Int. J. Dev Biol. 2002, 46, 49-63.
9. - channels in Xenopus oocytes injected with Torpedo electroplaque membranes. Proc. Natl. Acad. Sci. USA 1995, 92, 5224-5228.
10. Colman, A., in: Hames, B. D., Higgings, S. J. (Eds.), Transcription and Translation. A Practical Approach. IRL, Oxford-Washington, 1984, pp. 271-302.
11. Sigel, E., Properties of single sodium channels translated by Xenopus oocytes after injection with messenger ribonucleic acid. J. Physiol. (Lond.) 1987, 386, 73-90.
12. 1 subunit tagged with green fluorescent protein requires a β-subunit for functional surface expression. J. Biol. Chem. 1998, 273, 28906-28911.
13. A receptors isolated by selective immunoprecipitation from the cell surface. Neuropharmacology 1996, 35, 1403-1411.
14. Soreq, H., The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes. CRC Crit. Rev. Biochem. 1985, 18, 199-238.
15. Gundersen, C. B., Miledi, R., Parker, I., Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes. Nature 1984, 308, 421-424.
16. Noda, M., Ikeda, T., Suzuki, H., Takeshima, H., et al., Expression of functional sodium channels from cloned cDNA. Nature 1986, 322, 826-828.
17. Mishina, M., Kurosaki, T., Tobimatsu, T., Morimoto, Y., et al., Expression of functional acetylcholine receptor from cloned cDNAs. Nature 1984, 307, 604-608.
18. A receptors on channel function. Neuron 1990, 5, 703-711.
19. A receptor channels. EMBO J. 1992, 11, 2017-2023.
20. A receptors influence affinities for benzodiazepine binding site ligands. Mol. Pharmacol. 1997, 52, 676-682.
21. A receptors. Proc. Natl. Acad. Sci. USA 2004, 101, 7769-7774.
22. 2-subunit of γ-aminobutyric acid type A receptors results in altered benzodiazepine binding site specificity. Proc. Natl. Acad. Sci. USA 1997, 94, 8824-8829.
23. Nowak, M. W., Kearney, P. C., Sampson, J. R., Saks, M. E., et al., Nicotinic receptor binding site probed with unnatural amino acid incorporation in intact cells. Science 1995, 268, 439-442.
24. 1-subunit gene SCN1B. Nature Genetics 1998, 19, 366-370.
25. Restituito, S., Thompson, R. M., Eliet, J., Raike, R., et al., The polyglutamine expansion in spinocerebellar ataxia type 6 causes a β-subunit-specific enhanced activation of P/Q-type calcium channels in Xenopus oocytes. J. Neurosci. 2000, 20, 6394-6403.
26. Lehmann-Horn, F., Jurkat-Rott, K., Voltage-gated ion channels and hereditary disease. Physiol. Rev. 1999, 79, 1317-1372.
27. Kavvadias, D., Sand, P., Youdim, K. A., Qaiser, M. Z., Rice-Evans, C., Baur, R., Sigel, E., Rausch. W. D., Riederer, P., Schreier, P., The flavone hispidulin, a benzodiazepine receptor ligand with positive allosteric properties, traverses the blood-brain barrier and exhibits anticonvulsive effects. Br. J. Pharmacol. 2004, 142, 811-820.
28. Romero, M. F., Kanai, Y., Gunshin, H., Hediger, M. A., Expression cloning using Xenopus laevis oocytes. Methods Enzymol. 1998, 296, 17-52.
29. +/glucose co-transporter. Nature 1987, 330, 379-381.