Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2-D crystallization

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Galián, Carmen ^a,b,c   Manon, Florence ^a,b,c   Dezi, Manuela ^d,e   Torres, Cristina ^a,b,c   Ebel, Christine ^a,b,c   Lévy, Daniel ^d,e   Jault, Jean Michel ^a,b,c  

^a UNIV GRENOBLE ALPES   (France)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c CEA GRENOBLE   (France)

^d INSTITUT CURIE   (France)

^e CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; ADENOSINE TRIPHOSPHATASE; DETERGENT; HETERODIMER; OLIGOMER; PROTEIN BMRC; PROTEIN BMRD; UNCLASSIFIED DRUG; VANADIC ACID; BACTERIAL PROTEIN; BMR PROTEIN, BACILLUS SUBTILIS; CARRIER PROTEIN; DODECYL MALTOSIDE; GLUCOSIDE; LIPID;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; CRYSTALLIZATION; ELECTRON MICROSCOPY; ENZYME ACTIVITY; GEL FILTRATION; LIPID COMPOSITION; MOLECULAR WEIGHT; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN QUALITY; PROTEIN STABILITY; PROTEOLIPOSOME; SOLUBILIZATION; STRUCTURE ANALYSIS; SUPRAMOLECULAR CHEMISTRY; THERMOSTABILITY; ULTRACENTRIFUGATION; BACILLUS SUBTILIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; PROTEIN MULTIMERIZATION;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

ATP-BINDING CASSETTE TRANSPORTERS; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CRYSTALLIZATION; ESCHERICHIA COLI; GLUCOSIDES; LIPIDS; MEMBRANE TRANSPORT PROTEINS; MICROSCOPY, ELECTRON; PROTEIN MULTIMERIZATION; PROTEIN STABILITY;

EID: 79955934876     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0019677     Document Type: Article

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