Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Wu, Zhaoyun ^a   Zhao, Jinming ^a   Gao, Ruifang ^a   Hu, Guanjun ^a   Gai, Junyi ^a   Xu, Guohua ^a   Xing, Han ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOSPHATE; PHOSPHATE TRANSPORTER; PHOSPHATE TRANSPORTER 1; PHOSPHATE TRANSPORTER 2; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE TRANSLOCATION; GENETIC CODE; HYDROPHILICITY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE PHOSPHORYLATION; PHOSPHATE TRANSPORTER 1 GENE; PHOSPHATE TRANSPORTER 2 GENE; PLANT GENE; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEEDLING; SEQUENCE HOMOLOGY; SOYBEAN; CELL FRACTIONATION; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR GENETICS;

GLYCINE MAX; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, PLANT; MOLECULAR SEQUENCE DATA; PHOSPHATE TRANSPORT PROTEINS; PLANT PROTEINS; SOYBEANS; SUBCELLULAR FRACTIONS;

EID: 79958791220     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0019752     Document Type: Article

References (62)

1. Karandashov V, Bucher M, (2005) Symbiotic phosphate transport in arbuscular mycorrhizas. Trends Plant Sci 10: 22-29.
2. Rausch C, Bucher M, (2002) Molecular mechanisms of phosphate transport in plants. Planta 216: 23-37.
3. Yuan H, Liu D, (2008) Signaling components involved in plant responses to phosphate starvation. J Integr Plant Biol 50: 849-859.
4. Schachtman DP, Reid RJ, Ayling SM, (1998) Phosphorus Uptake by Plants: From Soil to Cell. Plant Physiol 116: 447-453.
5. Smith FW, Mudge SR, Rae AL, Glassop D, (2003) 2.Phosphate transport in plants. Plant Soil 248: 71-83.
6. Rausch C, Daram P, Brunner S, Jansa J, Laloi M, et al. (2001) A phosphate transporter expressed in arbuscule-containing cells in potato. Nature 414: 462-470.
7. Chiou TJ, Liu H, Harrison MJ, (2001) The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface. Plant J 25: 281-293.
8. Marschner H, (1995) Nutrient availability in soils. Mineral Nutrition of Higher Plants. 2nd ed London Academic Press pp. 483-507.
9. Gordon-Weeks R, Tong YP, Davies TGE, Leggewie G, (2003) Restricted spatial expression of a high-affinity phosphate transporter in potato roots. J Cell Sci 116: 3135-3144.
10. Ullrich-Eberius C, Novacky A, Bel A, (1984) Phosphate uptake inLemna gibba G1: energetics and kinetics. Planta 161: 46-52.
11. Raghothama KG, (1999) PHOSPHATE ACQUISITION. Annu Rev Plant Physiol Plant Mol Bio 50: 665-693.
12. Furihata T, Suzuki M, Sakurai H, (1992) Kinetic characterization of two phosphate uptake systems with different affinities in suspension-cultured Catharanthus roseus protoplasts. Plant Cell Physiol 33: 1151.
13. Muchhal US, Raghothama KG, (1999) Transcriptional regulation of plant phosphate transporters. Proc Natl Acad Sci U S A 96: 5868-5872.
14. Muchhal US, Pardo JM, Raghothama KG, (1996) Phosphate transporters from the higher plant Arabidopsis thaliana. Proc Natl Acad Sci U S A 93: 10519-10523.
15. Liu C, Muchhal US, Uthappa M, Kononowicz AK, Raghothama KG, (1998) Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus. Plant Physiol 116: 91-99.
16. Okumura S, Mitsukawa N, Shirano Y, Shibata D, (1998) Phosphate transporter gene family of Arabidopsis thaliana. DNA Res 5: 261-269.
17. Hamburger D, Rezzonico E, MacDonald-Comber Petetot J, Somerville C, Poirier Y, (2002) Identification and Characterization of the Arabidopsis PHO1 Gene Involved in Phosphate Loading to the Xylem. Plant Cell 14: 889-902.
18. Jeschke WD, Kirkby EA, Peuke AD, Pate JS, Hartung W, (1997) Effects of P deficiency on assimilation and transport of nitrate and phosphate in intact plants of castor bean (Ricinus communis L.). J Exp Bot 48: 75-91.
19. Versaw WK, Harrison MJ, (2002) A chloroplast phosphate transporter, PHT2;1, influences allocation of phosphate within the plant and phosphate-starvation responses. Plant Cell 14: 1751-1766.
20. Rae AL, Cybinski DH, Jarmey JM, Smith FW, (2003) Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family. Plant Mol Biol 53: 27-36.
21. Ai Penghui, Sun Shubin, Zhao Jianning, Fan Xiaorong, Xin Weijie, et al. (2009) Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation. Plant J 57: 798-809.
22. Shoemaker RC, Schlueter J, Doyle JJ, (2006) Paleopolyploidy and gene duplication in soybean and other legumes. Curr Opin Plant Biol 9: 104-109.
23. Graham PH, Vance CP, (2003) Legumes: Importance and Constraints to Greater Use. Plant Physiol 131: 872-877.
24. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
25. Smith FW, Ealing PM, Dong B, Delhaize E, (1997) The cloning of two Arabidopsis genes belonging to a phosphate transporter family. Plant J 11: 83-92.
26. Leggewie G, Willmitzer L, Riesmeier JW, (1997) Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: Identification of phosphate transporters from higher plants. Plant Cell 9: 381-392.
27. Liu H, Trieu AT, Blaylock LA, Harrison MJ, (1998) Cloning and characterization of two phosphate transporters from Medicago truncatula roots: Regulation in response to phosphate and to colonization by arbuscular mycorrhizal (AM) fungi. Mol Plant Microbe Interact 11: 14-22.
28. Bun-Ya M, Nishimura M, Harashima S, Oshima Y, (1991) The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol Cell Biol 11: 3229-3238.
29. Bun-ya M, Shikata K, Nakade S, Yompakdee C, Harashima S, et al. (1996) Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Curr Genet 29: 344-351.
30. Daram P, Brunner S, Persson BL, Amrhein N, Bucher M, (1998) Functional analysis and cell-specific expression of a phosphate transporter from tomato. Planta 206: 225-233.
31. Daram P, Brunner S, Rausch C, Steiner C, Amrhein N, et al. (1999) Pht2;1 encodes a low-affinity phosphate transporter from Arabidopsis. Plant Cell 11: 2153-2166.
32. Liu JQ, Uhde-Stone C, Li A, Vance C, Allan D, (2001) A phosphate transporter with enhanced expression in proteoid roots of white lupin (Lupinus albus L.). Plant Soil 237: 257-266.
33. Paszkowski U, Kroken S, Roux C, Briggs SP, (2002) Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis. Proc Natl Acad Sci U S A 99: 13324-13329.
34. Zhao L, Versaw WK, Liu J, Harrison MJ, (2003) A phosphate transporter from Medicago truncatula is expressed in the photosynthetic tissues of the plant and located in the chloroplast envelope. New Phytol 157: 291-302.
35. Nagy R, Vasconcelos MJV, Zhao S, McElver J, Bruce W, et al. (2006) Differential regulation of five Pht1 phosphate transporters from maize (Zea mays L.). Plant Biol (Stuttg) 8: 186-197.
36. Rashid M, Saha S, Raghava GPS, (2007) Support Vector Machine-based method for predicting subcellular localization of mycobacterial proteins using evolutionary information and motifs. BMC Bioinformatics 8: 337.
37. Epstein E, Hagen CE, (1952) A Kinetic Study of the Absorption of Alkali Cations by Barley Roots. Plant Physiol 27: 457-474.
38. Epstein E, Rains DW, Elzam OE, (1963) Resolution of Dual Mechanisms of Potassium Absorption by Barley Roots. Proc Natl Acad Sci U S A 49: 684-692.
39. Pao SS, Paulsen IT, Saier MH Jr, (1998) Major Facilitator Superfamily. Microbiol Mol Biol Rev 62: 1-34.
40. Bucher M, Rausch C, Daram P, (2001) Molecular and biochemical mechanisms of phosphorus uptake into plants. J Plant Nutr Soil Sci 164: 209-217.
41. Auesukaree C, Homma T, Kaneko Y, Harashima S, (2003) Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae. Biochem Biophys Res Commun 306: 843-850.
42. Ullrich-Eberius C, Novacky A, Fischer E, Luttge U, (1981) Relationship between energy-dependent phosphate uptake and the electrical membrane potential in Lemna gibba G1. Plant Physiol 67: 797-801.
43. Ullrich-Eberius C, Novacky A, Bel A, (1984) Phosphate uptake in Lemna gibba G1: energetics and kinetics. Planta 161: 46-52.
44. Sakano K, (1990) Proton/phosphate stoichiometry in uptake of inorganic phosphate by cultured cells of Catharanthus roseus (L.) G. Don. Plant Physiol 93: 479.
45. Harrison MJ, Dewbre GR, Liu J, (2002) A Phosphate Transporter from Medicago truncatula Involved in the Acquisition of Phosphate Released by Arbuscular Mycorrhizal Fungi. Plant Cell 14: 2413-2429.
46. Poirier Y, Thoma S, Somerville C, Schiefelbein J, (1991) Mutant of Arabidopsis deficient in xylem loading of phosphate. Plant Physiol 97: 1087-1093.
47. Dong B, Rengel Z, Delhaize E, (1998) Uptake and translocation of phosphate by pho2 mutant and wild-type seedlings of Arabidopsis thaliana. Planta 205: 251-256.
48. Delhaize E, Randall PJ, (1995) Characterization of a Phosphate-Accumulator Mutant of Arabidopsis thaliana. Plant Physiol 107: 207-213.
49. Liu F, Wang Z, Ren H, Shen C, Li Y, et al. (2010) OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice. Plant J 62: 508-517.
50. Mudge SR, Rae AL, Diatloff E, Smith FW, (2002) Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J 31: 341-353.
51. Deleage G, Combet C, Blanchet C, Geourjon C, (2001) ANTHEPROT: An integrated protein sequence analysis software with client/server capabilities. Comput Biol Med 31: 259-267.
52. Tusnády GE, Simon I, (2001) The HMMTOP transmembrane topology prediction server. Bioinformatics 17: 849-850.
53. Sigrist CJ, Cerutti L, de Castro E, Langendijk-Genevaux PS, Bulliard V, et al. (2010) PROSITE, a protein domain database for functional characterization and annotation. Nucleic Acids Res 38: D161-166.
54. Thompson JD, Higgins DG, Gibson TJ, (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22: 4673-4680.
55. Tamura K, Dudley J, Nei M, Kumar S, (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596-1599.
56. Riesmeier JW, Willmitzer L, Frommer WB, (1992) Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast. EMBO J 11: 4705-4713.
57. Gietz RD, Schiestl RH, (2007) High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protocols 2: 31-34.
58. Wykoff DD, O'Shea EK, (2001) Phosphate Transport and Sensing in Saccharomyces cerevisiae. Genetics 159: 1491-1499.
59. Libault M, Thibivilliers S, Bilgin DD, Radwan O, Benitez M, et al. (2008) Identification of Four Soybean Reference Genes for Gene Expression Normalization. The Plant Genome 1: 44-54.
60. Schmittgen TD, Livak KJ, (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protocols 3: 1101-1108.
61. Livak KJ, Schmittgen TD, (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408.
62. Kyte J, Doolittle R, (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105-132.