Transfer and expression of foreign genes in mammalian cells

BioTechniques

Volumn 29, Issue 2, 2000, Pages 314-331

  Colosimo, A ^a   Goncz, K K ^a   Holmes, A R ^a   Kunzelmann, K ^a   Novelli, G ^a   Malones, R W ^a   Bennett, M J ^a   Gruenert, D C ^a  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TRANSFECTION; ENHANCER REGION; EUKARYOTIC CELL; EXPRESSION VECTOR; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; GENE TRANSFER; HUMAN; MAMMAL CELL; NONHUMAN; PROMOTER REGION; REVIEW;

EUKARYOTA; MAMMALIA;

EID: 0033836072     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00292rv01     Document Type: Review

References (157)

1. The plasmid replicon of EBV consists of multiple cis-acting elements that facilitate DNA synthesis by cell and a viral maintenance element
2. Reporter genes: Application to the study of mammalian gene transcription
3. Introduction and expression of DNA molecules in eukaryotic cells by electroporation
4. Identification of amplified DNA sequences on double minute chromosomes in a leukemic cell line KY821 by means of spectral karyotyping and comparative genomic hybridization
5. Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery
6. Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein
7. An optimized electroporation protocol applicable to a wide range of cell lines
8. Construction and properties of an Epstein-Barr-virus-derived cDNA expression vector for human cells
9. Development of adenovirus vectors for the expression of heterologous genes
10. Treatment of severe combined immunodeficiency disease (SCID) due to adenosine
11. The β-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner
12. Status of gene therapy for cystic fibrosis lung disease
13. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
14. Baculovirus-mediated gene transfer into mammalian cells
15. Strontium phosphate transfection of human cells in primary culture: Stable expression of the simian virus 40 large T-antigen in primary human bronchial epithelial cells
16. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs
17. Adenovirus-mediated in vivo gene transfer
18. Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis
19. Pox viruses as expression vectors
20. Enhancement of polylysine-mediated transferrinfection by nuclear localization sequences: Polylysine does not function as a nuclear localization sequence
21. Design of protocols for electroporation and electrofusion: Selection of electrical parameters
22. Calcium phosphate-mediated gene transfer: A highly efficient transfection system for stably transforming cells with plasmid DNA
23. Current status of cationic liposome-mediated gene therapy in cystic fibrosis
24. Gene transfection efficiency of tracheal epithelial cells by Dc-Chol-DOPE/DNA complexes
25. Characterization of immortal cystic fibrosis tracheobronchial gland epithelial cells
26. CFTR expression and ion transport in polarized immortal humanbronchial epithelial cells
27. A transformed human epithelial cell line that retains tight junctions post crisis
28. Administration of an adenovirus containing the human CFTR cDNA to the respiratory tract of individuals with cystic fibrosis
29. High-efficiency gene transfer mediated by adenovirus-polylysine-DNA complexes
30. Methods for micromanipulation of human somatic cells in culture
31. A new DNA vehicle for nonviral gene delivery: Supercoiled minicircle
32. Minicircle: An improved DNA molecule for in vitro and in vivo gene transfer
33. Glass needle-mediated microinjection of macromolecules and trnasgenes into primary human blood stem/progenitor cels
34. Biodistribution tissue reaction and lung retention of pentamidine aerosolized as three different salts
35. Microinjecting DNA into mouse ova to study DNA replication and gene expression and to produce transgenic animals
36. Correction of the cystic fibrosis defect in vitro by retrovirus-mediated gene transfer
37. Correction of defective PKA regulation of outwardly rectifying chloride channels by insertion of cystic fibrosis transmembrane regulator into CF airway epithelial cells
38. Retroviral vectors for introduction of genes into mammalian cells
39. Sugar-mediated uptake of glycosylated polylysines and gene transfer into normal and cystic fibrosis airway epithelial cells
40. Effect of cationic cholesterol derivatives on gene transfer and protein kinase C activity
41. Complexes of adenovirus with polycationic polymers and cationic lipids increase the efficiency of gene transfer in vitro and in vivo
42. Impact of a new generation of gene transfer markers on gene therapy
43. Nonviral strategies for gene therapy
44. Gene transfer into the airway epithelium of animals by targeting the polymeric immunoglobulin receptor
45. The transmembrane domain of diphtheria toxin improves molecular conjugate gene transfer
46. A phase I study of an adeno-associated virus-CFTR gene vector in adult CF patients with mild lung disease
47. Gene therapy for cystic fibrosis
48. Adeno-associated virus vectors for gene therapy
49. Progress toward human gene therapy
50. Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes
51. An element in the bovine papillomavirus late 3' untranslated region reduces polyadenylated cytoplasmic RNA levels
52. Cationic liposome-mediated gene transfer
53. A placebo-controlled study of liposome-mediated gene transfer to the nasal epithelium of patients with cystic fibrosis
54. SV40-transformed simian cells support the replication of early SV40 mutants
55. Site-directed alteration of DNA by small fragment homologous replacement (SFHR)
56. Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate
57. Position-independent high-level expression of the human β-globin gene in transgenic mice
58. Retroviral vector-mediated gene transfer into human hematopoietic progenitor cells
59. Differentiated properties of human epithelial cells transformed in vitro
60. Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40
61. Immortalized cell cultures of CF-tissues as a tool to study ion transport properties
62. Polyamidoamine cascade polymers and their use for efficient transfection of cells in culture
63. Recombinant adeno-associated virus (rAAV) vectors for somatic gene therapy: Recent advances and potential clinical applications
64. Gene transfer into mammalian cells by particle bombardment
65. Do LCRs open chromatin domains?
66. Virosomes: Cationic liposomes enhance retroviral transduction
67. Efficient gene transfer into human hepatocytes by baculovirus vectors
68. Baculovirus-mediated gene transfer in the presence of human serum or blood facilitated by inhibition of the complement system
69. Intracellular compartmentalization of DNA fragments in cultured airway epithelial cells mediated by cationic lipids
70. The introduction of biologically active foreign genes into human respiratory epithelial cells using electroporation
71. Prospects for gene therapy in cystic fibrosis
72. Activation of a heterologous promoter in response to dexamethasone and cadmium by metallothionein gene 5'-flanking DNA
73. Tissue-specific targeting of retroviral vectors through ligand-receptor interactions
74. Locus control regions: Overcoming heterochromatin-induced gene activation in mammals
75. Particle bombardment: A universal approach for gene transfer to cells and tissues
76. The dose dependence of glucocoticoid-inducible gene expression results from changes in the number of transcriptionally active templates
77. Enhanced efficiency of lactosylated poly-L-lysine-mediated gene transfer into cystic fibrosis airway epithelial cells
78. Adenoviral vectors for gene transfer
79. Efficient transfer of genetic material into mammalian cells using Starbust polyamidoamine dendrimers
80. Gene targeting of CFTR DNA in CF epithelial cells
81. Liposomes in gene therapy
82. Agonist-mediated destabilization of m1 muscarinic acetylcholine receptor mRNA. Elements involved in mRNA stability are localized in the 3'-untranslated region
83. Lipidic vector system for gene transfer
84. Delivery of plasmid DNA into mammalian cell lines using pH-sensitive liposomes: Comparison with cationic liposomes
85. Episomal expression of wild-type CFTR corrects cAMP-dependent chloride transport in respiratory epithelial cells
86. A novel gene delivery system targeting cells expressing VEGF receptors
87. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment
88. Stable transduction of recombinant adeno-associated virus into hematopoietic stem cells from normal and sickle cell patients
89. Alphaviruses as expression vectors
90. High efficiency polyoma DNA transfection of chloroquine treated cells
91. A small region of the mouse mammary tumor virus long terminal repeat confers glucocorticoid hormone regulation on a linked heterologous gene
92. Lipofectin-mediated RNA transfection
93. Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells
94. Optimizing electroporation parameters for a variety of human hematopoietic cell lines
95. Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells
96. Efficient gene transfer by histidylated polylysine/pDNA complexes
97. Genetically engineered pox viruses for recombinant gene expression, vaccination, and safety
98. Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: A negative selection system
99. Gene transfer in vivo with DNA-liposome complexes: Lack of autoimmunity and gonadal localization
100. Direct gene transfer with DNA-liposome complexes in melanoma: Expression, biologic activity, and lack of toxicity in humans
101. Differential sensitivity of double minute chromosomes to hydroxyurea treatment in cultured methotrexate-resistant mouse cells
102. Site-specific integration in mammalian cells mediated by a new hybrid baculovirus-adeno-associated virus vector
103. Applications of pox virus vectors to vaccination: An update
104. The AU-rich sequences in the 3' untranslated region mediate the increased turnover of interferon mRNA induced by glucocorticoids
105. Gene transfer in vivo: Sustained expression and regulation of genes introduced into the liver by receptor-targeted uptake
106. Targeted integration of adeno-associated virus-derived plasmids in transfected human cells
107. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells
108. Adeno-associated virus type 2-mediated transduction of murine hematopoietic cells with long-term repopulating ability and sustained expression of a human globin gene in vivo
109. Hormonal response region in the mouse mammary tumor virus long terminal repeat can be dissociated from proviral promoter and has enhancer properties
110. Evidence for safety and efficacy of DOTAP cationic liposome mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis
111. Baculoviruses as expression vectors
112. Application of electroporation in recombinant DNA technology
113. Enhancer-dependent expression of human k immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation
114. Adeno-associated virus expression systems for gene transfer
115. A novel technique for the study of intercellular, junctional communication: Electroporation of adherent cells on a partly conductive slide
116. Transformation of human bronchial epithelial cells by infection with SV40 hybrid virus, or transfection via strontium phosphate coprecipitation with a plasmid containing SV40 early region genes
117. Recent advances in inducible gene expression systems
118. Human gene targeting by viral vectors
119. Inducible gene expression in mammalian cells and transgenic mice
120. Optimizing the biolistic process for different biological applications
121. Liposomal DNA vectors for cystic fibrosis gene therapy. Current applications, limitations, and future directions
122. A GC-rich domain with bifunctional effects on mRNA and protein levels: Implications for control of transforming growth factor beta 1 expression
123. DNA vector chemistry: The covalent attachment of signal peptides to plasmid DNA
124. Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency
125. Electroporation of eukaryotes and prokaryotes: A general approach to the introduction of macromolecules into cells
126. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors
127. Enhanced transformation of human fibroblasts by origin-defective SV40
128. Gene therapy for cystic fibrosis using cationic liposome mediated gene transfer: A phase I trial of safety and efficacy in the nasal airway
129. Studies on the transfer of DNA into cells through use of avidin-poly1ysine conjugates complexed to biotinylated transferrin and DNA
130. A vector that replicates as a plasmid and can be efficiently selected in B-lymphocytes transformed by Epstein-Barr virus
131. Low-voltage electric discharge biolistic device
132. In vitro gene delivery by degraded polyamidoamine dendrimers
133. Structure of polycation-DNA complexes and theories of DNA compactions
134. Improved DNA:Liposome complexes for increased systemic delivery and gene expression
135. High frequency targeting of genes to specific sites in the mammalian genome
136. Transient promoter activity in primary rat mammary epithelial cells evaluated using particle bombardment gene transfer
137. Novel vectors for gene delivery formed by self-assembly of DNA with poly(L-lysine) grafted with hydrophilic polymers
138. The 3'-untranslated region of mouse myelin basic protein gene increases the amount of mRNA in immortalized mouse oligodendrocytes
139. Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle
140. Effects of lipopolysaccharide on transfection efficiency in eukaryotic cells
141. Efficient purification of plasmid DNA for gene transfer using triple-helix affinity chromatography
142. Addition of glycerol during DNA exposure enhances calcium phospate transfection
143. In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment
144. Gene gun and other non-viral approaches for cancer gene therapy
145. Efficient in situ electroporation of mammalian cells grown on microporous membranes
146. Stable replication of a plasmid derived from Epstein-Barr virus in various mammalian cells
147. HVJ (Sendai virus)-cationic liposomes: A novel and potentially effective liposome-mediated technique for gene transfer to the airway epithelium
148. Adenovirus-mediated gene transfer transiently corrects the chloride transport defect in nasal epithelia of patients with cystic fibrosis
149. Cellular and molecular barriers to gene transfer by a cationic lipid
150. Transfer of foreign DNA into the cells of developing mouse embryos by microprojectile bombardment
151. Genetic transformation of mouse cultured cells with the help of high-velocity mechanical DNA injection
152. Intracellular distribution and mechanism of delivery of oligonucleotides mediated by cationic lipids
153. High-efficiency gene transfection by in situ electroporation of cultured cells