Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview

Methods in Molecular Biology

Volumn 738, Issue , 2011, Pages 199-216

  Katona, Robert L ^a   Vanderbyl, Sandra L ^b   Perez, Carl F ^b  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b NONE

Author keywords

Adult stem cells; Embryonic stem cells; Gene therapy; MACs; Mammalian artificial chromosomes; Transgenic animals

Indexed keywords

ANIMAL; ARTIFICIAL CHROMOSOME; BIOLOGICAL THERAPY; CELL LINE; CHROMOSOMAL INSTABILITY; CYTOLOGY; GENETIC ENGINEERING; GENETICS; HUMAN; METABOLISM; METHODOLOGY; REVIEW; STEM CELL; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELL LINE; CHROMOSOMAL INSTABILITY; CHROMOSOMES, ARTIFICIAL, MAMMALIAN; GENETIC ENGINEERING; HUMANS; STEM CELLS; TISSUE THERAPY;

EID: 79960912996     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-099-7_14     Document Type: Chapter

References (180)

1. Ascenzioni, F., Donini, P. and Lipps, H.J. (1997). Mammalian artificial chromosomes – vectors for somatic gene therapy. Cancer Lett. 118, 135–142.
2. Basu, J. and Willard, H.F. (2006). Human artificial chromosomes: potential applications and clinical considerations. Pediatr. Clin. North Am. 53, 843–853, viii-843–853, viii.
3. Bridger, J.M. (2004). Mammalian artificial chromosomes: modern day feats of engineering – Isambard Kingdom Brunel style. Cytogenet. Genome Res. 107, 5–8.
4. Cooke, H. (2001). Mammalian artificial chromosomes as vectors: progress and prospects. Cloning Stem Cells 3, 243–249.
5. Larin, Z. and Mejia, J.E. (2002). Advances in human artificial chromosome technology. Trends Genet. 18, 313–319.
6. Lewis, M. (2001). Human artificial chromosomes: emerging from concept to reality in biomedicine. Clin. Genet. 59, 15–16.
7. Lim, H.N. and Farr, C.J. (2004). Chromosome-based vectors for Mammalian cells: an overview. Methods Mol. Biol. 240, 167–186.
8. Monaco, Z.L. and Moralli, D. (2006). Progress in artificial chromosome technology. Biochem. Soc. Trans. 34, 324–327.
9. Ren, X., Tahimic, C.G.T., Katoh, M., Kurimasa, A., Inoue, T. and Oshimura, M. (2006). Human artificial chromosome vectors meet stem cells: new prospects for gene delivery. Stem Cell Rev 2, 43–50.
10. Ehrhardt, A., Haase, R., Schepers, A., Deutsch, M.J., Lipps, H.J. and Baiker, A. (2008). Episomal vectors for gene therapy. Curr Gene Ther 8, 147–161.
11. Macnab, S. and Whitehouse, A. (2009). Progress and prospects: human artificial chromosomes. Gene Ther. 16, 1180–1188.
12. Duncan, A. and Hadlaczky, G. (2007). Chromosomal engineering. Curr. Opin. Biotechnol. 18, 420–424.
13. Hadlaczky, G. (2001). Satellite DNA-based artificial chromosomes for use in gene therapy. Curr. Opin. Mol. Ther. 3, 125–132.
14. Grimes, B.R. and Monaco, Z.L. (2005). Artificial and engineered chromosomes: developments and prospects for gene therapy. Chromosoma 114, 230–241.
15. Basu, J. and Willard, H.F. (2005). Artificial and engineered chromosomes: nonintegrating vectors for gene therapy. Trends Mol Med 11, 251–258.
16. Brown, W.R., Mee, P.J. and Hong Shen, M. (2000). Artificial chromosomes: ideal vectors? Trends Biotechnol. 18, 218–223.
17. Lipps, H.J., Jenke, A.C., Nehlsen, K., Scinteie, M.F., Stehle, I.M. and Bode, J. (2003). Chromosome-based vectors for gene therapy. Gene 304, 23–33.
18. Grimes, B.R., Warburton, P.E. and Farr, C.J. (2002). Chromosome engineering: prospects for gene therapy. Gene Ther. 9, 713–718.
19. Harrington, J.J., Van Bokkelen, G., Mays, R.W., Gustashaw, K. and Willard, H.F. (1997). Formation of de novo centromeres and construction of first-generation human artificial microchromosomes. Nat. Genet. 15, 345–355.
20. Ikeno, M., Grimes, B., Okazaki, T., Nakano, M., Saitoh, K., Hoshino, H., McGill, N.I., Cooke, H. and Masumoto, H. (1998). Construction of YAC-based mammalian artificial chromosomes. Nat. Biotechnol. 16, 431–439.
21. Henning, K.A., Novotny, E.A., Compton, S.T., Guan, X.Y., Liu, P.P. and Ashlock, M.A. (1999). Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences. Proc Natl Acad Sci USA 96, 592–597.
22. de las Heras, J.I., D’Aiuto, L. and Cooke, H. (2004). Mammalian artificial chromosome formation in human cells after lipofection of a PAC precursor. Methods Mol. Biol. 240, 187–206.
23. Basu, J., Willard, H.F. and Stromberg, G. (2007). Human artificial chromosome assembly by transposon-based retrofitting of genomic BACs with synthetic alpha-satellite arrays. Curr Protoc Hum Genet Chapter 5, Unit 5.18-Unit 5.18.
24. Nakashima, H., Nakano, M., Ohnishi, R., Hiraoka, Y., Kaneda, Y., Sugino, A. and Masumoto, H. (2005). Assembly of additional heterochromatin distinct from centromerekinetochore chromatin is required for de novo formation of human artificial chromosome. J. Cell. Sci. 118, 5885–5898.
25. Kaname, T., McGuigan, A., Georghiou, A., Yurov, Y., Osoegawa, K., De Jong, P.J., Ioannou, P. and Huxley, C. (2005). Alphoid DNA from different chromosomes forms de novo minichromosomes with high efficiency. Chromosome Res. 13, 411–422.
26. Basu, J., Compitello, G., Stromberg, G., Willard, H.F. and Van Bokkelen, G. (2005). Efficient assembly of de novo human artificial chromosomes from large genomic loci. BMC. Biotechnol 5, 21–21.
27. Grimes, B.R., Rhoades, A.A. and Willard, H.F. (2002). Alpha-satellite DNA and vector composition influence rates of human artificial chromosome formation. Mol. Ther. 5, 798–805.
28. Ikeno, M., Suzuki, N., Hasegawa, Y. and Okazaki, T. (2009). Manipulating transgenes using a chromosome vector. Nucleic Acids Res. 37, e44–e44.
29. Kouprina, N., Ebersole, T., Koriabine, M., Pak, E., Rogozin, I.B., Katoh, M., Oshimura, M., Ogi, K., Peredelchuk, M., Solomon, G., Brown, W., Barrett, J.C. and Larionov, V. (2003). Cloning of human centromeres by transformation-associated recombination in yeast and generation of functional human artificial chromosomes. Nucleic Acids Res. 31, 922–934.
30. Suzuki, N., Nishii, K., Okazaki, T. and Ikeno, M. (2006). Human artificial chromosomes constructed using the bottom-up strategy are stably maintained in mitosis and efficiently transmissible to progeny mice. J. Biol. Chem 281, 26615–26623.
31. Carine, K., Solus, J., Waltzer, E., Manch Citron, J., Hamkalo, B.A. and Scheffler, I.E. (1986). Chinese hamster cells with a minichromosome containing the centromere region of human chromosome 1. Somat. Cell Mol. Genet. 12, 479–491.
32. Farr, C.J., Bayne, R.A., Kipling, D., Mills, W., Critcher, R. andCooke, H.J. (1995). Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation. EMBO J. 14, 5444–5454.
33. Heller, R., Brown, K.E., Burgtorf, C. and Brown, W.R. (1996). Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage. Proc Natl Acad Sci USA 93, 7125–7130.
34. Kuroiwa, Y., Tomizuka, K., Shinohara, T., Kazuki, Y., Yoshida, H., Ohguma, A., Yamamoto, T., Tanaka, S., Oshimura, M. and Ishida, I. (2000). Manipulation of human minichromosomes to carry greater than megabase-sized chromosome inserts. Nat. Biotechnol 18, 1086–1090.
35. Au, H.C., Mascarello, J.T. and Scheffler, I.E. (1999). Targeted integration of a dominant neo(R) marker into a 2-to 3-Mb human minichromosome and transfer between cells. Cytogenet. Cell Genet. 86, 194–203.
36. Mills, W., Critcher, R., Lee, C. and Farr, C.J. (1999). Generation of an approximately 2.4 Mb human X centromere-based minichromosome by targeted telomere-associated chromosome fragmentation in DT40. Hum. Mol. Genet. 8, 751–761.
37. Shen, M.H., Mee, P.J., Nichols, J., Yang, J., Brook, F., Gardner, R.L., Smith, A.G. and Brown, W.R. (2000). A structurally defined mini-chromosome vector for the mouse germ line. Curr. Biol. 10, 31–34.
38. Choo, K.H., Saffery, R., Wong, L.H., Irvine, D.V., Bateman, M.A., Griffiths, B., Cutts, S.M., Cancilla, M.R., Cendron, A.C. and Stafford, A.J. (2001). Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation. Proc Natl Acad Sci USA 98, 5705–5710.
39. Voet, T., Vermeesch, J., Carens, A., Durr, J., Labaere, C., Duhamel, H., David, G. and Marynen, P. (2001). Efficient male and female germline transmission of a human chromosomal vector in mice. Genome Res. 11, 124–136.
40. Wong, L.H., Saffery, R. and Choo, K.H.A. (2002). Construction of neocentromerebased human minichromosomes for gene delivery and centromere studies. Gene Ther. 9, 724–726.
41. Auriche, C., Donini, P. and Ascenzioni, F. (2001). Molecular and cytological analysis of a 5.5 Mb minichromosome. EMBO Rep. 2, 102–107.
42. Katoh, M., Ayabe, F., Norikane, S., Okada, T., Masumoto, H., Horike, S., Shirayoshi, Y. and Oshimura, M. (2004). Construction of a novel human artificial chromosome vector for gene delivery. Biochem. Biophys. Res. Commun. 321, 280–290.
43. Hadlaczky, G., Praznovszky, T., Cserpan, I., Kereso, J., Peterfy, M., Kelemen, I., Atalay, E., Szeles, A., Szelei, J. and Tubak, V. (1991). Centromere formation in mouse cells cotransformed with human DNA and a dominant marker gene. Proc. Natl. Acad. Sci. U.S.A 88, 8106–8110.
44. Praznovszky, T., Kereső, J., Tubak, V., Cserpán, I., Fátyol, K. and Hadlaczky, G. (1991). De novo chromosome formation in rodent cells. Proc Natl Acad Sci USA 88, 11042–11046.
45. Kereso, J., Praznovszky, T., Cserpan, I., Fodor, K., Katona, R., Csonka, E., Fatyol, K., Hollo, G., Szeles, A., Ross, A.R., Sumner, A.T., Szalay, A.A. and Hadlaczky, G. (1996). De novo chromosome formations by largescale amplification of the centromeric region of mouse chromosomes. Chromosome Res. 4, 226–239.
46. Csonka, E., Cserpan, I., Fodor, K., Hollo, G., Katona, R., Kereso, J., Praznovszky, T., Szakal, B., Telenius, A., deJong, G., Udvardy, A. and Hadlaczky, G. (2000). Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells. J. Cell Sci 113 (Pt 18), 3207–3216.
47. Hollo, G., Kereso, J., Praznovszky, T., Cserpan, I., Fodor, K., Katona, R., Csonka, E., Fatyol, K., Szeles, A., Szalay, A.A. and Hadlaczky, G. (1996). Evidence for a megareplicon covering megabases of centromeric chromosome segments. Chromosome Res. 4, 240–247.
48. Lindenbaum, M., Perkins, E., Csonka, E., Fleming, E., Garcia, L., Greene, A., Gung, L., Hadlaczky, G., Lee, E., Leung, J., MacDonald, N., Maxwell, A., Mills, K., Monteith, D., Perez, C.F., Shellard, J., Stewart, S., Stodola, T., Vandenborre, D., Vanderbyl, S. and Ledebur, H.C. (2004). A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical protein production, transgenesis and gene-based cell therapy. Nucleic Acids Res. 32, e172–e172.
49. Perez, C.F., Vanderbyl, S.L., Mills, K. and Ledebur Jr, H.C. (2004). The ACE System: A versatile chromosome engineering technology with applications for genebased cell therapy. Bioprocessing 3, 61–68.
50. Grimes, B.R., Schindelhauer, D., McGill, N.I., Ross, A., Ebersole, T.A. and Cooke, H.J. (2001). Stable gene expression from a mammalian artificial chromosome. EMBO Rep. 2, 910–914.
51. Breman, A.M., Steiner, C.M., Slee, R.B. and Grimes, B.R. (2008). Input DNA ratio determines copy number of the 33 kb Factor IX gene on de novo human artificial chromosomes. Mol. Ther. 16, 315–323.
52. Grimes, B.R., Babcock, J., Rudd, M.K., Chadwick, B. and Willard, H.F. (2004). Assembly and characterization of heterochromatin and euchromatin on human artificial chromosomes. Genome Biol. 5, R89–R89.
53. Rudd, M.K., Mays, R.W., Schwartz, S. and Willard, H.F. (2003). Human artificial chromosomes with alpha satellite-based de novo centromeres show increased frequency of nondisjunction and anaphase lag. Mol. Cell. Biol. 23, 7689–7697.
54. Higgins, A.W., Gustashaw, K.M. and Willard, H.F. (2005). Engineered human dicentric chromosomes show centromere plasticity. Chromosome Res. 13, 745–762.
55. Oshimura, M. and Katoh, M. (2008). Transfer of human artificial chromosome vectors into stem cells. Reprod. Biomed. Online 16, 57–69.
56. Ayabe, F., Katoh, M., Inoue, T., Kouprina, N., Larionov, V. and Oshimura, M. (2005). A novel expression system for genomic DNA loci using a human artificial chromosome vector with transformation-associated recombination cloning. J. Hum. Genet 50, 592–599.
57. Kawahara, M., Inoue, T., Ren, X., Sogo, T., Yamada, H., Katoh, M., Ueda, H., Oshimura, M. and Nagamune, T. (2007). Antigenmediated growth control of hybridoma cells via a human artificial chromosome. Biochim. Biophys. Acta 1770, 206–212.
58. Kakeda, M., Hiratsuka, M., Nagata, K., Kuroiwa, Y., Kakitani, M., Katoh, M., Oshimura, M. and Tomizuka, K. (2005). Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts. Gene Ther. 12, 852–856.
59. Otsuki, A., Tahimic, C.G., Tomimatsu, N., Katoh, M., Chen, D.J., Kurimasa, A. and Oshimura, M. (2005). Construction of a novel expression system on a human artificial chromosome. Biochem. Biophys. Res. Commun 329, 1018–1025.
60. Suda, T., Katoh, M., Hiratsuka, M., Takiguchi, M., Kazuki, Y., Inoue, T. and Oshimura, M. (2006). Heat-regulated production and secretion of insulin from a human artificial chromosome vector. Biochem. Biophys. Res. Commun. 340, 1053–1061.
61. Messina, G., Cossu, G., Oshimura, M., Hoshiya, H., Kazuki, Y., Abe, S., Takiguchi, M., Kajitani, N., Watanabe, Y., Yoshino, T., Shirayoshi, Y. and Higaki, K. (2009). A highly stable and nonintegrated human artificial chromosome (HAC) containing the 2.4 Mb entire human dystrophin gene. Mol. Ther. 17, 309–317.
62. Yamada, H., Li, Y.C., Nishikawa, M., Oshimura, M. and Inoue, T. (2008). Introduction of a CD40L genomic fragment via a human artificial chromosome vector permits cell-type-specific gene expression and induces immunoglobulin secretion. J. Hum. Genet. 53, 447–453.
63. Inoue, K., Kajitani, N., Yoshino, T., Shirayoshi, Y., Ogura, A., Shinohara, T., Barrett, J.C., Oshimura, M., Kazuki, Y., Hoshiya, H., Kai, Y., Abe, S., Takiguchi, M., Osaki, M., Kawazoe, S., Katoh, M. and Kanatsu-Shinohara, M. (2008). Correction of a genetic defect in multipotent germline stem cells using a human artificial chromosome. Gene Ther. 15, 617–624.
64. Oshimura, M., Tomizuka, K., Shitara, S., Kakeda, M., Nagata, K., Hiratsuka, M., Sano, A., Osawa, K., Okazaki, A., Katoh, M. and Kazuki, Y. (2008). Telomerasemediated lifespan extension of human primary fibroblasts by human artificial chromosome (HAC) vector. Biochem. Biophys. Res. Commun. 369, 807–811.
65. Sands, V.E. (1969). Short arm enlargement in acrocentric chromosomes. Am. J. Hum. Genet. 21, 293–304.
66. Warburton, D., Atwood, K.C. and Henderson, A.S. (1976). Variation in the number of genes for rRNA among human acrocentric chromosomes: correlation with frequency of satellite association. Cytogenet. Cell Genet. 17, 221–230.
67. Verma, R.S., Dosik, H. and Lubs, H.A. (1977). Size variation polymorphisms of the short arm of human acrocentric chromosomes determined by R-banding by fluorescence using acridine orange (RFA). Hum. Genet. 38, 231–234.
68. Sofuni, T., Tanabe, K. and Awa, A.A. (1980). Chromosome heteromorphisms in the Japanese. II. Nucleolus organizer regions of variant chromosomes in D and G groups. Hum. Genet. 55, 265–270.
69. Trask, B., van den Engh, G., Mayall, B. and Gray, J.W. (1989). Chromosome heteromorphism quantified by high-resolution bivariate flow karyotyping. Am. J. Hum. Genet. 45, 739–752.
70. Trask, B., van den Engh, G. and Gray, J.W. (1989). Inheritance of chromosome heteromorphisms analyzed by high-resolution bivariate flow karyotyping. Am. J. Hum. Genet. 45, 753–760.
71. Stergianou, K., Gould, C.P., Waters, J.J. and Hultén, M.A. (1993). A DA/DAPI positive human 14p heteromorphism defined by fluorescence in-situ hybridisation using chromosome 15-specific probes D15Z1 (satellite III) and p-TRA-25 (alphoid). Hereditas 119, 105–110.
72. Friedrich, U., Caprani, M., Niebuhr, E., Therkelsen, A.J. and Jørgensen, A.L. (1996). Extreme variant of the short arm of chromosome 15. Hum. Genet. 97, 710–713.
73. Conte, R.A., Kleyman, S.M., Laundon, C. and Verma, R.S. (1997). Characterization of two extreme variants involving the short arm of chromosome 22: are they identical? Ann. Genet. 40, 145–149.
74. Cooper, H.L. and Hirschhorn, K. (1962). Enlarged satellites as a familial chromosome marker. Am. J. Hum. Genet. 14, 107–124.
75. Therkelsen, A.J. (1964). Enlarged short arm of a small acrocentric chromosome in grandfather, mother and child, the latter with Down’s syndrome. Cytogenetics 10, 441–451.
76. Yoder, F.E., Bias, W.B., Borgaonkar, D.S., Bahr, G.F., Yoder, I.I., Yoder, O.C. and Golomb, H.M. (1974). Cytogenetic and linkage studies of a familial 15pplus variant. Am. J. Hum. Genet. 26, 535–548.
77. Bernstein, R., Dawson, B. and Griffiths, J. (1981). Human inherited marker chromosome 22 short-arm enlargement: investigation of rDNA gene multiplicity, Agband size, and acrocentric association. Hum. Genet. 58, 135–139.
78. Schmid, M., Nanda, I., Steinlein, C. and Epplen, J.T. (1994). Amplification of (GACA)n simple repeats in an exceptional 14p+ marker chromosome. Hum. Genet. 93, 375–382.
79. Mukerjee, D. and Burdette, W.J. (1966). A familial minute isochromosome. Am. J. Hum. Genet. 18, 62–69.
80. Howard-Peebles, P.N. (1979). A familial bisatellited extra metacentric microchromosome in man. J. Hered. 70, 347–348.
81. Blennow, E., Bui, T.H., Kristoffersson, U., Vujic, M., Annerén, G., Holmberg, E. and Nordenskjöld, M. (1994). Swedish survey on extra structurally abnormal chromosomes in 39 105 consecutive prenatal diagnoses: prevalence and characterization by fluorescence in situ hybridization. Prenat. Diagn. 14, 1019–1028.
82. Brondum-Nielsen, K. and Mikkelsen, M. (1995). A 10-year survey, 1980-1990, of prenatally diagnosed small supernumerary marker chromosomes, identified by FISH analysis. Outcome and follow-up of 14 cases diagnosed in a series of 12,699 prenatal samples. Prenat. Diagn. 15, 615–619.
83. Crolla, J.A., Howard, P., Mitchell, C., Long, F.L. and Dennis, N.R. (1997). A molecular and FISH approach to determining karyotype and phenotype correlations in six patients with supernumerary marker(22) chromosomes. Am. J. Med. Genet. 72, 440–447.
84. Adhvaryu, S.G., Peters-Brown, T., Livingston, E. and Qumsiyeh, M.B. (1998). Familial supernumerary marker chromosome evolution through three generations. Prenat. Diagn. 18, 178–181.
85. Hadlaczky, G., Bujdosó, G., Koltai, E., Schwanitz, G. and Csonka, E. (2002). A natural equivalent to human satellite DNAbased artificial chromosome persists over 140 years, in a three-generation family. European Journal of Human Genetics 10 (Supplement), 143–143.
86. Kennard, M.L., Goosney, D.L., Monteith, D., Zhang, L., Moffat, M., Fischer, D. and Mott, J. (2009). The generation of stable, high MAb expressing CHO cell lines based on the artificial chromosome expression (ACE) technology. Biotechnol. Bioeng. 104, 540–553.
87. Kennard, M.L., Goosney, D.L., Monteith, D., Roe, S., Fischer, D. and Mott, J. (2009). Auditioning of CHO host cell lines using the artificial chromosome expression (ACE) technology. Biotechnol. Bioeng. 104, 526–539.
88. Katona, R.L., Sinkó, I., Holló, G., Szucs, K.S., Praznovszky, T., Kereső, J., Csonka, E., Fodor, K., Cserpán, I., Szakál, B., Blazsó, P., Udvardy, A. and Hadlaczky, G. (2008). A combined artificial chromosome-stem cell therapy method in a model experiment aimed at the treatment of Krabbe’s disease in the Twitcher mouse. Cell. Mol. Life Sci. 65, 3830–3838.
89. Adriaansen, J., Vervoordeldonk, M.J., Vanderbyl, S., de Jong, G. and Tak, P.P. (2006). A novel approach for gene therapy: engraftment of fibroblasts containing the artificial chromosome expression system at the site of inflammation. J Gene Med 8, 63–71.
90. Schor, S.L., Johnson, R.T. and Mullinger, A.M. (1975). Perturbation of mammalian cell division. II. Studies on the isolation and characterization of human mini segregant cells. J. Cell. Sci. 19, 281–303.
91. Fournier, R.E. and Ruddle, F.H. (1977). Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells. Proc Natl Acad Sci USA 74, 319–323.
92. Meaburn, K.J., Parris, C.N. and Bridger, J.M. (2005). The manipulation of chromosomes by mankind: the uses of microcell mediated chromosome transfer. Chromosoma 114, 263–274.
93. Doherty, A.M.O. and Fisher, E.M.C. (2003). Microcell-mediated chromosome transfer (MMCT): small cells with huge potential. Mamm. Genome 14, 583–592.
94. Yamada, H., Kunisato, A., Kawahara, M., Tahimic, C.G., Ren, X., Ueda, H., Nagamune, T., Katoh, M., Inoue, T., Nishikawa, M. and Oshimura, M. (2006). Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome. J. Hum .Genet 51, 147–150.
95. de Jong, G., Telenius, A.H., Telenius, H., Perez, C.F., Drayer, J.I. and Hadlaczky, G. (1999). Mammalian artificial chromosome pilot production facility: large-scale isolation of functional satellite DNA-based artificial chromosomes. Cytometry 35, 129–133.
96. Co, D.O., Borowski, A.H., Leung, J.D., van der Kaa, J., Hengst, S., Platenburg, G.J., Pieper, F.R., Perez, C.F., Jirik, F.R. and Drayer, J.I. (2000). Generation of transgenic mice and germline transmission of a mammalian artificial chromosome introduced into embryos by pronuclear microinjection. Chromosome Res. 8, 183–191.
97. Monteith, D.P., Leung, J.D., Borowski, A.H., Co, D.O., Praznovszky, T., Jirik, F.R., Hadlaczky, G. and Perez, C.F. (2004). Pronuclear microinjection of purified artificial chromosomes for generation of transgenic mice: pick-and-inject technique. Methods Mol. Biol. 240, 227–242.
98. Vanderbyl, S., MacDonald, G.N., Sidhu, S., Gung, L., Telenius, A., Perez, C. and Perkins, E. (2004). Transfer and stable transgene expression of a mammalian artificial chromosome into bone marrow-derived human mesenchymal stem cells. Stem Cells 22, 324–333.
99. Vanderbyl, S.L., Sullenbarger, B., White, N., Perez, C.F., MacDonald, G.N., Stodola, T., Bunnell, B.A., Ledebur, H.C. and Lasky, L.C. (2005). Transgene expression after stable transfer of a mammalian artificial chromosome into human hematopoietic cells. Exp. Hematol. 33, 1470–1476.
100. de Jong, G., Telenius, A., Vanderbyl, S., Meitz, A. and Drayer, J. (2001). Efficient invitro transfer of a 60-Mb mammalian artificial chromosome into murine and hamster cells using cationic lipids and dendrimers. Chromosome Res. 9, 475–485.
101. Robl, J.M., Kasinathan, P., Sullivan, E., Kuroiwa, Y., Tomizuka, K. and Ishida, I. (2003). Artificial chromosome vectors and expression of complex proteins in transgenic animals. Theriogenology 59, 107–113.
102. Kuroiwa, Y., Kasinathan, P., Choi, Y.J., Naeem, R., Tomizuka, K., Sullivan, E.J., Knott, J.G., Duteau, A., Goldsby, R.A., Osborne, B.A., Ishida, I. and Robl, J.M. (2002). Cloned transchromosomic calves producing human immunoglobulin. Nat. Biotechnol 20, 889–894.
103. Kazuki, Y., Shinohara, T., Tomizuka, K., Katoh, M., Ohguma, A., Ishida, I. and Oshimura, M. (2001). Germline transmission of a transferred human chromosome 21 fragment in transchromosomal mice. J. Hum. Genet. 46, 600–603.
104. Raper, S.E., Chirmule, N., Lee, F.S., Wivel, N.A., Bagg, A., Gao, G., Wilson, J.M. and Batshaw, M.L. (2003). Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer. Mol. Genet. Metab. 80, 148–158.
105. Christ, M., Lusky, M., Stoeckel, F., Dreyer, D., Dieterlé, A., Michou, A.I., Pavirani, A. and Mehtali, M. (1997). Gene therapy with recombinant adenovirus vectors: evaluation of the host immune response. Immunol. Lett. 57, 19–25.
106. Couto, L.B. (2004). Preclinical gene therapy studies for hemophilia using adenoassociated virus (AAV) vectors. Semin. Thromb. Hemost. 30, 161–171.
107. Miller, D.G., Rutledge, E.A. and Russell, D.W. (2002). Chromosomal effects of adeno-associated virus vector integration. Nat. Genet. 30, 147–148.
108. Miller, D.G., Petek, L.M. and Russell, D.W. (2004). Adeno-associated virus vectors integrate at chromosome breakage sites. Nat. Genet. 36, 767–773.
109. Hacein-Bey-Abina, S., von Kalle, C., Schmidt, M., McCormack, M.P., Wulffraat, N., Leboulch, P., Lim, A., Osborne, C.S., Pawliuk, R., Morillon, E., Sorensen, R., Forster, A., Fraser, P., Cohen, J.I., de Saint, B.G., Alexander, I., Wintergerst, U., Frebourg, T., Aurias, A., Stoppa-Lyonnet, D., Romana, S., Radford-Weiss, I., Gross, F., Valensi, F., Delabesse, E., Macintyre, E., Sigaux, F., Soulier, J., Leiva, L.E., Wissler, M., Prinz, C., Rabbitts, T.H., Le Deist, F., Fischer, A. and Cavazzana-Calvo, M. (2003). LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCIDX1. Science 302, 415–419.
110. Cavazzana-Calvo, M., Fischer, A., Hacein Bey-Abina, S., von Kalle, C., Schmidt, M., Le Deist, F., Wulffraat, N., McIntyre, E., Radford, I., Villeval, J. and Fraser, C.C. (2003). A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency. N. Engl. J. Med. 348, 255–256.
111. Guhr, A., Kurtz, A., Friedgen, K. and La ser, P. (2006). Current state of human embryonic stem cell research: an overview of cell lines and their use in experimental work. Stem Cells 24, 2187–2191.
112. Guan, K., Nayernia, K., Maier, L.S., Wagner, S., Dressel, R., Lee, J.H., Nolte, J., Wolf, F., Li, M., Engel, W. and Hasenfuss, G. (2006). Pluripotency of spermatogonial stem cells from adult mouse testis. Nature 440, 1199–1203.
113. Shamblott, M.J., Axelman, J., Wang, S., Bugg, E.M., Littlefield, J.W., Donovan, P.J., Blumenthal, P.D., Huggins, G.R. and Gearhart, J.D. (1998). Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc. Natl. Acad. Sci. U.S.A. 95, 13726–13731.
114. Donovan, P.J. and de Miguel, M.P. (2003). Turning germ cells into stem cells. Curr. Opin. Genet. Dev. 13, 463–471.
115. Klimanskaya, I., Chung, Y., Becker, S., Lu, S.J. and Lanza, R. (2006). Human embryonic stem cell lines derived from single blastomeres. Nature 444, 481–485.
116. Hochedlinger, K. and Jaenisch, R. (2006). Nuclear reprogramming and pluripotency. Nature 441, 1061–1067.
117. Takahashi, K. and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663–676.
118. Evans, M.J. and Kaufman, M.H. (1981). Establishment in culture of pluripotential cells from mouse embryos. Nature 292, 154–156.
119. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S. and Jones, J.M. (1998). Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147.
120. Orimo, A., Gupta, P.B., Sgroi, D.C., Arenzana-Seisdedos, F., Delaunay, T., Naeem, R., Carey, V.J., Richardson, A.L. and Weinberg, R.A. (2005). Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Cell 121, 335–348.
121. Hung, S.C., Deng, W.P., Yang, W.K., Liu, R.S., Lee, C.C., Su, T.C., Lin, R.J., Yang, D.M., Chang, C.W., Chen, W.H., Wei, H.J. and Gelovani, J.G. (2005). Mesenchymal stem cell targeting of microscopic tumors and tumor stroma development monitored by noninvasive in vivo positron emission tomography imaging. Clin. Cancer Res. 11, 7749–7756.
122. Danks, M.K., Yoon, K.J., Bush, R.A., Remack, J.S., Wierdl, M., Tsurkan, L., Kim, S.U., Garcia, E., Metz, M.Z., Najbauer, J., Potter, P.M. and Aboody, K.S. (2007). Tumortargeted enzyme/prodrug therapy mediates long-term disease-free survival of mice bearing disseminated neuroblastoma. Cancer Res. 67, 22–25.
123. Aboody, K.S., Najbauer, J., Schmidt, N.O., Yang, W., Wu, J.K., Zhuge, Y., Przylecki, W., Carroll, R., Black, P.M. and Perides, G. (2006). Targeting of melanoma brain metastases using engineered neural stem/progenitor cells. Neuro-oncology 8, 119–126.
124. Aboody, K.S., Bush, R.A., Garcia, E., Metz, M.Z., Najbauer, J., Justus, K.A., Phelps, D.A., Remack, J.S., Yoon, K.J., Gillespie, S., Kim, S.U., Glackin, C.A., Potter, P.M. and Danks, M.K. (2006). Development of a tumor-selective approach to treat metastatic cancer. PLoS ONE 1, e23–e23.
125. Studeny, M., Marini, F.C., Dembinski, J.L., Zompetta, C., Cabreira-Hansen, M., Bekele, B.N., Champlin, R.E. and Andreeff, M. (2004). Mesenchymal stem cells: potential precursors for tumor stroma and targeteddelivery vehicles for anticancer agents. J. Natl. Cancer Inst. 96, 1593–1603.
126. Zhu, W., Xu, W., Jiang, R., Qian, H., Chen, M., Hu, J., Cao, W., Han, C. and Chen, Y. (2006). Mesenchymal stem cells derived from bone marrow favor tumor cell growth in vivo. Exp. Mol. Pathol. 80, 267–274.
127. Nakayama, N., Lee, J. and Chiu, L. (2000). Vascular endothelial growth factor synergistically enhances bone morphogenetic protein4-dependent lymphohematopoietic cell generation from embryonic stem cells in vitro. Blood 95, 2275–2283.
128. Kyba, M., Perlingeiro, R.C.R. and Daley, G.Q. (2002). HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Cell 109, 29–37.
129. Kondo, M., Wagers, A.J., Manz, M.G., Prohaska, S.S., Scherer, D.C., Beilhack, G.F., Shizuru, J.A. and Weissman, I.L. (2003). Biology of hematopoietic stem cells and progenitors: implications for clinical application. Annu. Rev. Immunol. 21, 759–806.
130. Burt, R.K., Verda, L., Kim, D.A., Oyama, Y., Luo, K. and Link, C. (2004). Embryonic stem cells as an alternate marrow donor source: engraftment without graft-versus-host disease. J. Exp. Med. 199, 895–904.
131. Park, C., Afrikanova, I., Chung, Y.S., Zhang, W.J., Arentson, E., Fong Gh, G.H., Rosendahl, A. and Choi, K. (2004). A hierarchical order of factors in the generation of FLK1-and SCL-expressing hematopoietic and endothelial progenitors from embryonic stem cells. Development 131, 2749–2762.
132. Doetschman, T.C., Eistetter, H., Katz, M., Schmidt, W. and Kemler, R. (1985). The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol 87, 27–45.
133. Bautch, V.L., Stanford, W.L., Rapoport, R., Russell, S., Byrum, R.S. and Futch, T.A. (1996). Blood island formation in attached cultures of murine embryonic stem cells. Dev. Dyn. 205, 1–12.
134. Yamashita, J., Itoh, H., Hirashima, M., Ogawa, M., Nishikawa, S., Yurugi, T., Naito, M., Nakao, K. and Nishikawa, S. (2000). Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors. Nature 408, 92–96.
135. Kolossov, E., Fleischmann, B.K., Liu, Q., Bloch, W., Viatchenko-Karpinski, S., Manzke, O., Ji, G.J., Bohlen, H., Addicks, K. and Hescheler, J. (1998). Functional characteristics of ES cell-derived cardiac precursor cells identified by tissue-specific expression of the green fluorescent protein. J. Cell Biol. 143, 2045–2056.
136. Yang, Y., Min, J.Y., Rana, J.S., Ke, Q., Cai, J., Chen, Y., Morgan, J.P. and Xiao, Y.F. (2002). VEGF enhances functional improvement of postinfarcted hearts by transplantation of ESC-differentiated cells. J. Appl. Physiol. 93, 1140–1151.
137. Min, J.Y., Yang, Y., Converso, K.L., Liu, L., Huang, Q., Morgan, J.P. and Xiao, Y.F. (2002). Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats. J. Appl. Physiol. 92, 288–296.
138. Müller, M., Fleischmann, B.K., Selbert, S., Ji, G.J., Endl, E., Middeler, G., Müller, O.J., Schlenke, P., Frese, S., Wobus, A.M., Hescheler, J., Katus, H.A. and Franz, W.M. (2000). Selection of ventricular-like cardiomyocytes from ES cells in vitro. FASEB J. 14, 2540–2548.
139. Zandstra, P.W., Bauwens, C., Yin, T., Liu, Q., Schiller, H., Zweigerdt, R., Pasumarthi, K.B.S. and Field, L.J. (2003). Scalable production of embryonic stem cellderived cardiomyocytes. Tissue Eng. 9, 767–778.
140. Hidaka, K., Lee, J.K., Kim, H.S., Ihm, C.H., Iio, A., Ogawa, M., Nishikawa, S.I., Kodama, I. and Morisaki, T. (2003). Chamber-specific differentiation of Nkx2.5-positive cardiac precursor cells from murine embryonic stem cells. FASEB J. 17, 740–742.
141. Min, J.Y., Yang, Y., Sullivan, M.F., Ke, Q., Converso, K.L., Chen, Y., Morgan, J.P. and Xiao, Y.F. (2003). Long-term improvement of cardiac function in rats after infarction by transplantation of embryonic stem cells. J. Thorac. Cardiovasc. Surg. 125, 361–369.
142. Pinto, D.O., Kolterud, A. and Carlsson, L. (1998). Expression of the LIMhomeobox gene LH2 generates immortalized steel factor-dependent multipotent hematopoietic precursors. EMBO J. 17, 5744–5756.
143. Lieber, J.G., Webb, S., Suratt, B.T., Young, S.K., Johnson, G.L., Keller, G.M. and Worthen, G.S. (2004). The in vitro production and characterization of neutrophils from embryonic stem cells. Blood 103, 852–859.
144. Eto, K., Murphy, R., Kerrigan, S.W., Bertoni, A., Stuhlmann, H., Nakano, T., Leavitt, A.D. and Shattil, S.J. (2002). Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc. Natl. Acad. Sci. U.S.A. 99, 12819–12824.
145. Tsai, M., Wedemeyer, J., Ganiatsas, S., Tam, S.Y., Zon, L.I. and Galli, S.J. (2000). In vivo immunological function of mast cells derived from embryonic stem cells: an approach for the rapid analysis of even embryonic lethal mutations in adult mice in vivo. Proc. Natl. Acad. Sci. U.S.A. 97, 9186–9190.
146. Hamaguchi-Tsuru, E., Nobumoto, A., Hirose, N., Kataoka, S., Fujikawa-Adachi, K., Furuya, M. and Tominaga, A. (2004). Development and functional analysis of eosinophils from murine embryonic stem cells. Br. J. Haematol. 124, 819–827.
147. Fairchild, P.J., Nolan, K.F., Cartland, S., GraA a, L. and Waldmann, H. (2003). Stable lines of genetically modified dendritic cells from mouse embryonic stem cells. Transplantation 76, 606–608.
148. Carotta, S., Pilat, S., Mairhofer, A., Schmidt, U., Dolznig, H., Steinlein, P. and Beug, H. (2004). Directed differentiation and mass cultivation of pure erythroid progenitors from mouse embryonic stem cells. Blood 104, 1873–1880.
149. Chang, A.H., Stephan, M.T. and Sadelain, M. (2006). Stem cell-derived erythroid cells mediate long-term systemic protein delivery. Nat. Biotechnol. 24, 1017–1021.
150. Colman, A. (2004). Making new beta cells from stem cells. Semin. Cell Dev. Biol. 15, 337–345.
151. Stoffel, M., Vallier, L. and Pedersen, R.A. (2004). Navigating the pathway from embryonic stem cells to beta cells. Semin. Cell Dev. Biol. 15, 327–336.
152. D’Amour, K.A., Agulnick, A.D., Eliazer, S., Kelly, O.G., Kroon, E. and Baetge, E.E. (2005). Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. Biotechnol. 23, 1534–1541.
153. D’Amour, K.A., Bang, A.G., Eliazer, S., Kelly, O.G., Agulnick, A.D., Smart, N.G., Moorman, M.A., Kroon, E., Carpenter, M.K. and Baetge, E.E. (2006). Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat. Biotechnol. 24, 1392–1401.
154. Hamazaki, T., Iiboshi, Y., Oka, M., Papst, P.J., Meacham, A.M., Zon, L.I. and Terada, N. (2001). Hepatic maturation in differentiating embryonic stem cells in vitro. FEBS Lett. 497, 15–19.
155. Jones, E.A., Tosh, D., Wilson, D.I., Lindsay, S. and Forrester, L.M. (2002). Hepatic differentiation of murine embryonic stem cells. Exp. Cell Res. 272, 15–22.
156. Yamada, T., Yoshikawa, M., Kanda, S., Kato, Y., Nakajima, Y., Ishizaka, S. and Tsunoda, Y. (2002). In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green. Stem Cells 20, 146–154.
157. Lin, R.Y., Kubo, A., Keller, G.M. and Davies, T.F. (2003). Committing embryonic stem cells to differentiate into thyrocyte-like cells in vitro. Endocrinology 144, 2644–2649.
158. Ali, N.N., Edgar, A.J., Samadikuchaksaraei, A., Timson, C.M., Romanska, H.M., Polak, J.M. and Bishop, A.E. (2002). Derivation of type II alveolar epithelial cells from murine embryonic stem cells. Tissue Eng. 8, 541–550.
159. Yamada, T., Yoshikawa, M., Takaki, M., Torihashi, S., Kato, Y., Nakajima, Y., Ishizaka, S. and Tsunoda, Y. (2002). In vitro functional gut-like organ formation from mouse embryonic stem cells. Stem Cells 20, 41–49.
160. Brustle, O., Spiro, A.C., Karram, K., Choudhary, K., Okabe, S. and McKay, R.D. (1997). In vitro-generated neural precursors participate in mammalian brain development. Proc. Natl. Acad. Sci. U.S.A 94, 14809–14814.
161. Brustle, O., Jones, K.N., Learish, R.D., Karram, K., Choudhary, K., Wiestler, O.D., Duncan, I.D. and McKay, R.D. (1999). Embryonic stem cell-derived glial precursors: a source of myelinating transplants. Science 285, 754–756.
162. Kim, J.H., Auerbach, J.M., Rodríguez Gómez, J.A., Velasco, I., Gavin, D., Lumelsky, N., Lee, S.H., Nguyen, J., Sánchez-Pernaute, R., Bankiewicz, K. and McKay, R. (2002). Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson’s disease. Nature 418, 50–56.
163. Barberi, T., Klivenyi, P., Calingasan, N.Y., Lee, H., Kawamata, H., Loonam, K., Perrier, A.L., Bruses, J., Rubio, M.E., Topf, N., Tabar, V., Harrison, N.L., Beal, M.F., Moore, M.A.S. and Studer, L. (2003). Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice. Nat. Biotechnol. 21, 1200–1207.
164. Tropepe, V., Hitoshi, S., Sirard, C., Mak, T.W., Rossant, J. and van der Kooy, D. (2001). Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism. Neuron 30, 65–78.
165. Bagutti, C., Wobus, A.M., Fa ssler, R. and Watt, F.M. (1996). Differentiation of embryonal stem cells into keratinocytes: comparison of wild-type and beta 1 integrindeficient cells. Dev. Biol. 179, 184–196.
166. Coraux, C., Hilmi, C., Rouleau, M., Spadafora, A., Hinnrasky, J., Ortonne, J.P., Dani, C. and Aberdam, D. (2003). Reconstituted skin from murine embryonic stem cells. Curr. Biol. 13, 849–853.
167. Young, H.E., Duplaa, C., Katz, R., Thompson, T., Hawkins, K.C., Boev, A.N., Henson, N.L., Heaton, M., Sood, R., Ashley, D., Stout, C., Morgan, J.H., Uchakin, P.N., Rimando, M., Long, G.F., Thomas, C., Yoon, J.I., Park, J.E., Hunt, D.J., Walsh, N.M., Davis, J.C., Lightner, J.E., Hutchings, A.M., Murphy, M.L., Boswell, E., McAbee, J.A., Gray, B.M., Piskurich, J., Blake, L., Collins, J.A., Moreau, C., Hixson, D., Bowyer, F.P. and Black, A.C.J. (2005). Adult-derived stem cells and their potential for use in tissue repair and molecular medicine. J. Cell. Mol. Med. 9, 753–769.
168. Serafini, M., Dylla, S.J., Oki, M., Heremans, Y., Tolar, J., Jiang, Y., Buckley, S.M., Pelacho, B., Burns, T.C., Frommer, S., Rossi, D.J., Bryder, D., Panoskaltsis-Mortari, A., O’Shaughnessy, M.J., Nelson-Holte, M., Fine, G.C., Weissman, I.L., Blazar, B.R. and Verfaillie, C.M. (2007). Hematopoietic reconstitution by multipotent adult progenitor cells: precursors to long-term hematopoietic stem cells. J. Exp. Med. 204, 129–139.
169. Petrovic, V. and Stefanovic, V. (2009). Dental tissue–new source for stem cells. ScientificWorldJournal 9, 1167–1177.
170. Fraser, J.K., Wulur, I., Alfonso, Z. and Hedrick, M.H. (2006). Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol. 24, 150–154.
171. De Coppi, P., Bartsch, G., Siddiqui, M.M., Xu, T., Santos, C.C., Perin, L., Mostoslavsky, G., Serre, A.C., Snyder, E.Y., Yoo, J.J., Furth, M.E., Soker, S. and Atala, A. (2007). Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotechnol. 25, 100–106.
172. Auriche, C., Carpani, D., Conese, M., Caci, E., Zegarra-Moran, O., Donini, P. and Ascenzioni, F. (2002). Functional human CFTR produced by a stable minichromosome. EMBO Rep. 3, 862–868.
173. Bunnell, B.A., Izadpanah, R., Ledebur Jr, H.C. and Perez, C.F. (2005). Development of mammalian artificial chromosomes for the treatment of genetic diseases: Sandhoff and Krabbe diseases. Expert Opin Biol Ther 5, 195–206.
174. Ren, X., Katoh, M., Hoshiya, H., Kurimasa, A., Inoue, T., Ayabe, F., Shibata, K., Toguchida, J. and Oshimura, M. (2005). A novel human artificial chromosome vector provides effective cell lineage-specific transgene expression in human mesenchymal stem cells. Stem Cells 23, 1608–1616.
175. Goldman, S. (2005). Stem and progenitor cell-based therapy of the human central nervous system. Nat. Biotechnol. 23, 862–871.
176. Klug, M.G., Soonpaa, M.H., Koh, G.Y. and Field, L.J. (1996). Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts. J. Clin. Invest. 98, 216–224.
177. Li, M., Pevny, L., Lovell-Badge, R. and Smith, A. (1998). Generation of purified neural precursors from embryonic stem cells by lineage selection. Curr. Biol. 8, 971–974.
178. Billon, N., Jolicoeur, C., Ying, Q.L., Smith, A. and Raff, M. (2002). Normal timing of oligodendrocyte development from genetically engineered, lineage-selectable mouse ES cells. J. Cell. Sci. 115, 3657–3665.
179. Gilad, A.A., McMahon, M.T., Walczak, P., Winnard, P.T.J., Raman, V., van Laarhoven, H.W.M., Skoglund, C.M., Bulte, J.W.M. and van Zijl, P.C.M. (2007). Artificial reporter gene providing MRI contrast based on proton exchange. Nat. Biotechnol. 25, 217–219.
180. Gyöngyösi, M., Blanco, J., Marian, T., Trón, L., Petneházy, O., Petrasi, Z., Hemetsberger, R., Rodriguez, J., Font, G., Pavo, I.J., Kertész, I., Balkay, L., Pavo, N., Posa, A., Emri, M., Galuska, L., Kraitchman, D.L., Wojta, J., Huber, K. and Glogar, D. (2008). Serial noninvasive in vivo positron emission tomographic tracking of percutaneously intramyocardially injected autologous porcine mesenchymal stem cells modified for transgene reporter gene expression. Circ Cardiovasc Imaging 1, 94–103.