메뉴 건너뛰기




Volumn 285, Issue 5428, 1999, Pages 754-756

Embryonic stem cell-derived glial precursors: A source of myelinating transplants

Author keywords

[No Author keywords available]

Indexed keywords

GLIAL FIBRILLARY ACIDIC PROTEIN; PROTEOLIPID PROTEIN;

EID: 0033618593     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5428.754     Document Type: Article
Times cited : (885)

References (39)
  • 1
    • 0019826665 scopus 로고
    • M. J. Evans and M. H. Kaufman, Nature 292, 154 (1981); G. R. Martin, Proc. Natl. Acad. Sci. U.S.A. 78, 7634 (1981).
    • (1981) Nature , vol.292 , pp. 154
    • Evans, M.J.1    Kaufman, M.H.2
  • 3
    • 0032491416 scopus 로고    scopus 로고
    • J. A. Thomson et al., Science 282, 1145 (1998); M. J. Shamblott et al., Proc. Natl. Acad. Sci U.S.A. 95, 13726 (1998).
    • (1998) Science , vol.282 , pp. 1145
    • Thomson, J.A.1
  • 7
    • 0028841640 scopus 로고
    • G. Keller, Curr. Opin. Cell Biol. 7, 862 (1995); G. Bain, D. Kitchens, M. Yao, J. E. Huettner, D. I. Gottlieb, Dev. Biol. 168, 342 (1995); A. Fraichard et al., J. Cell Sci. 108, 3181 (1995); M. F. A. Finley, N. Kulkami, J. E. Huettner, J. Neurosci. 16, 1056 (1996); M. Li, L. Pevny, R. Lovell-Badge, A. Smith, Curr. Biol. 8, 971 (1998).
    • (1995) Curr. Opin. Cell Biol. , vol.7 , pp. 862
    • Keller, G.1
  • 8
    • 0028907753 scopus 로고
    • G. Keller, Curr. Opin. Cell Biol. 7, 862 (1995); G. Bain, D. Kitchens, M. Yao, J. E. Huettner, D. I. Gottlieb, Dev. Biol. 168, 342 (1995); A. Fraichard et al., J. Cell Sci. 108, 3181 (1995); M. F. A. Finley, N. Kulkami, J. E. Huettner, J. Neurosci. 16, 1056 (1996); M. Li, L. Pevny, R. Lovell-Badge, A. Smith, Curr. Biol. 8, 971 (1998).
    • (1995) Dev. Biol. , vol.168 , pp. 342
    • Bain, G.1    Kitchens, D.2    Yao, M.3    Huettner, J.E.4    Gottlieb, D.I.5
  • 9
    • 0029147524 scopus 로고
    • G. Keller, Curr. Opin. Cell Biol. 7, 862 (1995); G. Bain, D. Kitchens, M. Yao, J. E. Huettner, D. I. Gottlieb, Dev. Biol. 168, 342 (1995); A. Fraichard et al., J. Cell Sci. 108, 3181 (1995); M. F. A. Finley, N. Kulkami, J. E. Huettner, J. Neurosci. 16, 1056 (1996); M. Li, L. Pevny, R. Lovell-Badge, A. Smith, Curr. Biol. 8, 971 (1998).
    • (1995) J. Cell Sci. , vol.108 , pp. 3181
    • Fraichard, A.1
  • 10
    • 0029985691 scopus 로고    scopus 로고
    • G. Keller, Curr. Opin. Cell Biol. 7, 862 (1995); G. Bain, D. Kitchens, M. Yao, J. E. Huettner, D. I. Gottlieb, Dev. Biol. 168, 342 (1995); A. Fraichard et al., J. Cell Sci. 108, 3181 (1995); M. F. A. Finley, N. Kulkami, J. E. Huettner, J. Neurosci. 16, 1056 (1996); M. Li, L. Pevny, R. Lovell-Badge, A. Smith, Curr. Biol. 8, 971 (1998).
    • (1996) J. Neurosci. , vol.16 , pp. 1056
    • Finley, M.F.A.1    Kulkami, N.2    Huettner, J.E.3
  • 11
    • 0032572734 scopus 로고    scopus 로고
    • G. Keller, Curr. Opin. Cell Biol. 7, 862 (1995); G. Bain, D. Kitchens, M. Yao, J. E. Huettner, D. I. Gottlieb, Dev. Biol. 168, 342 (1995); A. Fraichard et al., J. Cell Sci. 108, 3181 (1995); M. F. A. Finley, N. Kulkami, J. E. Huettner, J. Neurosci. 16, 1056 (1996); M. Li, L. Pevny, R. Lovell-Badge, A. Smith, Curr. Biol. 8, 971 (1998).
    • (1998) Curr. Biol. , vol.8 , pp. 971
    • Li, M.1    Pevny, L.2    Lovell-Badge, R.3    Smith, A.4
  • 12
    • 0030894092 scopus 로고    scopus 로고
    • R. McKay, Science 276, 66 (1997); O. Bögler, D. Wren, S. C. Barnett, H. Land, M. Noble, Proc. Natl. Acad. Sci. U.S.A. 87, 6368 (1990).
    • (1997) Science , vol.276 , pp. 66
    • McKay, R.1
  • 14
    • 0027462920 scopus 로고    scopus 로고
    • A. K. Groves et al., Nature 362, 453 (1993); W. F. Blakemore, N. J. Olby, R. J. M. Franklin, Brain Pathol. 5, 443 (1995); F. Lachapelle, ibid., p. 289; I. D. Duncan, W. E. Grever, S. C. Zhang, Mol. Med. Today 3, 554 (1997).
    • (1993) Nature , vol.362 , pp. 453
    • Groves, A.K.1
  • 15
    • 0028849310 scopus 로고
    • A. K. Groves et al., Nature 362, 453 (1993); W. F. Blakemore, N. J. Olby, R. J. M. Franklin, Brain Pathol. 5, 443 (1995); F. Lachapelle, ibid., p. 289; I. D. Duncan, W. E. Grever, S. C. Zhang, Mol. Med. Today 3, 554 (1997).
    • (1995) Brain Pathol. , vol.5 , pp. 443
    • Blakemore, W.F.1    Olby, N.J.2    Franklin, R.J.M.3
  • 16
    • 0027462920 scopus 로고    scopus 로고
    • A. K. Groves et al., Nature 362, 453 (1993); W. F. Blakemore, N. J. Olby, R. J. M. Franklin, Brain Pathol. 5, 443 (1995); F. Lachapelle, ibid., p. 289; I. D. Duncan, W. E. Grever, S. C. Zhang, Mol. Med. Today 3, 554 (1997).
    • Brain Pathol. , pp. 289
    • Lachapelle, F.1
  • 17
    • 0031465925 scopus 로고    scopus 로고
    • A. K. Groves et al., Nature 362, 453 (1993); W. F. Blakemore, N. J. Olby, R. J. M. Franklin, Brain Pathol. 5, 443 (1995); F. Lachapelle, ibid., p. 289; I. D. Duncan, W. E. Grever, S. C. Zhang, Mol. Med. Today 3, 554 (1997).
    • (1997) Mol. Med. Today , vol.3 , pp. 554
    • Duncan, I.D.1    Grever, W.E.2    Zhang, S.C.3
  • 18
    • 0345151194 scopus 로고    scopus 로고
    • note
    • ES cells [line J1 (21), passage number ≤ 17] were grown on γ-irradiated embryonic fibroblasts in Dulbecco's modified Eagle's medium (DMEM) containing 20% fetal bovine serum, 0.1 mM 2-mercaptoethanol, nucleosides, nonessential amino acids, and human recombinant leukemia inhibitory factor (LIF, Life Technologies; 1000 units/ml). Cells were passaged once onto gelatin-coated dishes and then aggregated to form embryoid bodies in the absence of LIF. We plated 4-day-old embryoid bodies in tissue culture dishes and propagated them for 5 days in ITSFn medium [DMEM/F12 supplemented with 5 μg/ml insulin, 50 μg/ml transferrin, 30 nM selenium chloride, and 5 μg/ml fibronectin (8)]. Cells were then trypsinized, plated in polyomithine-coated dishes (15 μg/ml), and propagated in DMEM/F12 supplemented with insulin (25 μg/ml), transferrin (50 μg/ml), progesterone (20 nM), putrescine (100 μM), selenium chloride (30 nM) plus FGF2 (10 ng/ml), and laminin (1 μg/ml). After 5 days, cells were harvested by scraping in calcium- and magnesium-free Hanks' buffered salt solution (CMF-HBSS). triturated to a single-cell suspension, replated at a 1:5 ratio, and grown to subconfluency in the presence of FGF2 (10 ng/ml) and ECF (20 ng/ml). Cells were then passaged at a 1:5 ratio and again grown to subconfluency in the presence of FGF2 (10 ng/ml) and PDGF-AA (10 ng/ml). Human recombinant FGF2, EGF, and PDGF-AA (R&D Systems, Minneapolis, MN) were added daily and the medium was replaced every 2 days. In vitro differentiation was induced by growth factor withdrawal.
  • 21
    • 0345151192 scopus 로고    scopus 로고
    • note
    • Astrocytic and oligodendroglial differentiation were assessed by immunofluorescence analysis using antibodies to A2B5 (Boehringer; 1:200), O4 (Boehringer; 1:5) and GFAP (ICN; 1:100). Similar results were obtained using the ES cell line CJ7 (22) (passage number ≤ 18). Four days after growth factor withdrawal, 31.4 ± 3.6% and 35.0 ± 7.4% of the cells were immunoreactive to the O4 and GFAP antibodies, respectively (n = 5).
  • 24
    • 0344720895 scopus 로고    scopus 로고
    • note
    • Cells growing in medium containing FGF2 and PDGF were harvested and triturated to a single-cell suspension. Spinal cord transplants were performed as described (23). Following laminectomy at the thoracolumbar transition, 100,000 cells in a volume of 1 μl CMF-HBSS were injected into the dorsal column of 7-day-old md rats at one or several levels. The recipient animals received dally i.p. injections of cydosporin (10 mg/kg body weight). All procedures were done in accordance with institutional guidelines.
  • 26
    • 0345151190 scopus 로고    scopus 로고
    • note
    • Transplant recipients were perfusion-fixed with 4% paraformaldehyde at 3 weeks of age. Brains and spinal cords were processed for vibratome sectioning. Selected spinal cord transplant recipients were processed for semi- and ultrathin sectioning by routine procedures. Primary antibodies used for immunohistochemistry were anti-PLP (1:500), anti-MBP (Boehringer; 1:200), anti-CNP (Sigma; 1:200), and anti-GFAP (ICN; 1:100). Antigens were visualized using appropriate peroxidase-conjugated secondary antibodies. Identification of donor cells by DNA in situ hybridization with a probe to mouse satellite DNA was done as described (16).
  • 28
    • 0031790755 scopus 로고    scopus 로고
    • O. Brüstle, U. Maskos, R. D. G. McKay, Neuron 15, 1275 (1995); O. Brüstle et al., Nature Biotechnol. 16, 1040 (1998).
    • (1998) Nature Biotechnol. , vol.16 , pp. 1040
    • Brüstle, O.1
  • 29
    • 0028917250 scopus 로고
    • G. Fishell, Development 121, 803 (1995); K. Campbell, M. Olsson, A. Björklund, Neuron 15, 1259 (1995).
    • (1995) Development , vol.121 , pp. 803
    • Fishell, G.1
  • 31
    • 0345583042 scopus 로고    scopus 로고
    • note
    • 5 cells in a volume of 4 to 9 μl were injected into the ventricle of E17 rat embryos as described (16).
  • 32
    • 0024245022 scopus 로고
    • A. G. Smith et al., Nature 336, 688 (1988).
    • (1988) Nature , vol.336 , pp. 688
    • Smith, A.G.1
  • 33
    • 0031434399 scopus 로고    scopus 로고
    • O. Brüstle et al., Proc. Natl. Acad. Sci. U.S.A. 94, 14809 (1997); J. Dinsmore et al., Cell Transplant. 5, 131 (1996); T. Deacon, J. Dinsmore, L. C. Costantini, J. Ratliff, O. Isacson, Exp. Neurol. 149, 28 (1998).
    • (1997) Proc. Natl. Acad. Sci. U.S.A. , vol.94 , pp. 14809
    • Brüstle, O.1
  • 34
    • 0029867290 scopus 로고    scopus 로고
    • O. Brüstle et al., Proc. Natl. Acad. Sci. U.S.A. 94, 14809 (1997); J. Dinsmore et al., Cell Transplant. 5, 131 (1996); T. Deacon, J. Dinsmore, L. C. Costantini, J. Ratliff, O. Isacson, Exp. Neurol. 149, 28 (1998).
    • (1996) Cell Transplant. , vol.5 , pp. 131
    • Dinsmore, J.1
  • 39
    • 0345151189 scopus 로고    scopus 로고
    • note
    • We thank I. Griffiths for the PLP antibody and R. Buschwald for excellent technical assistance. This work was supported by a Junior Research Award from the state of Nordrhein-Westfalen (O.B.), the BONFOR program (O.B.), and NIH grant NS33710 (I.D.D.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.