Efficient assembly of de novo human artificial chromosomes from large genomic loci

BMC Biotechnology

Volumn 5, Issue , 2005, Pages

  Basu, Joydeep ^a,b   Compitello, George ^b   Stromberg, Gregory ^b   Willard, Huntington F ^a   Van Bokkelen, Gil ^b  

^a DUKE UNIVERSITY   (United States)

^b ATHERSYS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CHROMOSOMES; CLONING; DNA; GENE TRANSFER; MOLECULAR WEIGHT;

BIOMOLECULAR SYSTEM; HUMAN ARTIFICIAL CHROMOSOMES (HACS); HUMAN GROWTH HORMONE;

GENETIC ENGINEERING;

BETA GLOBIN; CIS ACTING ELEMENT; FIBROCYSTIN; GENOMIC DNA; HUMAN GROWTH HORMONE; DNA; GLOBIN; MESSENGER RNA; SATELLITE DNA;

ARTICLE; ARTIFICIAL CHROMOSOME; BACTERIAL ARTIFICIAL CHROMOSOME; CONTROLLED STUDY; EXPRESSION VECTOR; FREQUENCY ANALYSIS; GENE CONSTRUCT; GENE EXPRESSION; GENE FUNCTION; GENE LOCUS; GENE THERAPY; GENETIC SELECTION; HUMAN; HUMAN CELL; METHODOLOGY; NUCLEOTIDE SEQUENCE; VALIDATION PROCESS; BIOLOGICAL MODEL; BIOTECHNOLOGY; CELL LINE; CYTOLOGY; FIBROBLAST; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GENE TRANSFER; GENE VECTOR; GENETIC PROCEDURES; GENETIC TRANSFECTION; GENETICS; GENOME; KIDNEY POLYCYSTIC DISEASE; METABOLISM; MOLECULAR CLONING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); MAMMALIA;

BIOTECHNOLOGY; CELL LINE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CHROMOSOMES, ARTIFICIAL, HUMAN; CLONING, MOLECULAR; DNA; DNA, SATELLITE; FIBROBLASTS; GENE TRANSFER TECHNIQUES; GENETIC TECHNIQUES; GENETIC VECTORS; GENOME; GLOBINS; HUMAN GROWTH HORMONE; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, FLUORESCENCE; MODELS, GENETIC; POLYCYSTIC KIDNEY DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSFECTION;

EID: 26844440951     PISSN: 14726750     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-5-21     Document Type: Article

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