Construction of neocentromere-based human minichromosomes for gene delivery and centromere studies

Gene Therapy

Volumn 9, Issue 11, 2002, Pages 724-726

  Wong, L H ^a   Saffery, R ^a   Choo, K H A ^a  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

Author keywords

CENP A; Gene delivery; Human engineered chromosomes; Neocentromere

Indexed keywords

CHROMOSOME PROTEIN; SATELLITE DNA;

ARTICLE; CENTROMERE; CHROMOSOME MARKER; CONTROLLED STUDY; DNA VECTOR; EUKARYOTE; GENE TARGETING; GENE THERAPY; HUMAN; HUMAN CELL; HUMAN CHROMOSOME; MINICHROMOSOME; MITOSIS; MOLECULAR SIZE; PRIORITY JOURNAL; TELOMERE;

CENTROMERE; CHROMOSOMES, ARTIFICIAL, HUMAN; GENE DELETION; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENETIC ENGINEERING; HUMANS; MICROSATELLITE REPEATS; RESEARCH;

EUKARYOTA;

EID: 0036274392     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301756     Document Type: Article

References (17)

1. A functional marker centromere with no detectable alpha-satellite, satellite III, or CENP-B protein: Activation of a latent centromere?
2. A functional neo-centromere formed through activation of a latent human centromere and consisting of non-alpha-satellite DNA
3. Sequence analysis of an 80 kb human neocentromere
4. The 10q25 neocentromere and its inactive progenitor have identical primary nucleotide sequence: Further evidence for epigenetic modification
5. Components of the human spindle checkpoint control mechanism localize specifically to the active centromere on dicentric chromosomes
6. Human centromeres and neocentromeres show identical distribution patterns of >20 functionally important kinetochore-associated proteins
7. A 330 kb CENP-A binding domain and altered replication timing at a human neocentromere
8. A novel chromatin immunoprecipitation and array (CIA) analysis identifies a 460-kb CENP-A-binding neocentromere DNA
9. Domain organisation at the centromere and neocentromere
10. Molecular cytogenetic analysis of eight inversion duplications of human chromosome 13q that each contain a neocentromere
11. Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation
12. Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage
13. Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation
14. Artificial chromosomes: Ideal vectors?
15. Neocentromeres and human artificial chromosomes: An unnatural act
16. Engineering human chromosomes for gene therapy studies
17. Strategies for engineering human chromosomes with therapeutic potential