Molecular farming of antibodies in plants

Recent Advances in Plant Biotechnology

Volumn , Issue , 2009, Pages 35-63

  Fischer, Rainer ^a   Schillberg, Stefan ^a   Twyman, Richard M ^b  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^b UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 77956622512     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-1-4419-0194-1_3     Document Type: Chapter

References (142)

1. Andersen, D. C., Krummen, L. 2003. Recombinant protein expression for therapeutic applications. Curr. Opin. Biotechnol. 3:117-123.
2. Artsaenko, O., Kettig, B., Fiedler, U., Conrad, U., Düring, K. 1998. Potato tubers as a biofactory for recombinant antibodies. Mol. Breeding 4:313-319.
3. Bai, Y., Glatz, C. E. 2003. Bioprocess considerations for expanded-bed chromatography of crude canola extract: sample preparation and adsorbent reuse. Biotechnol. Bioeng. 81:775-782.
4. Bakker, H., Bardor, M., Molthoff, J. W., Gomord, V., Elbers, I., Stevens, L. H., Jordi, W., Lommen, A., Faye, L., Lerouge, P., Bosch, D. 2001. Galactose-extended glycans of antibodies produced by transgenic plants. Proc. Natl Acad. Sci. USA 98:2899-2904.
5. Baneyx, F., Mujacic, M. 2004. Recombinant protein folding and misfolding in Escherichia coli. Nat. Biotechnol. 22:1399-1408.
6. Basaran, P., Rodriguez-Cerezo, E. 2008. Plant molecular farming: opportunities and challenges. Crit. Rev. Biotechnol. 28:153-172.
7. Benvenuto, E., Ordas, R. J., Tavazza, R., Ancora, G., Biocca, S., Cattaneo, A., Galeffi, P. 1991. 'Phytoantibodies': a general vector for the expression of immunoglobulin domains in transgenic plants. Plant Mol. Biol. 17:865-874.
8. Blixt, O., Allin, K., Pereira, L., Datta, A., Paulson, J. C. 2002. Efficient chemoenzymatic synthesis of O-linked sialyl oligosaccharides. J. Am. Chem. Soc. 124:5739-5746.
9. Bock, R. 2007. Plastid biotechnology: prospects for herbicide and insect resistance, metabolic engineering and molecular farming. Curr. Opin. Biotechnol. 18:100-106.
10. Borisjuk, N. V., Borisjuk, L. G., Logendra, S., Petersen, F., Gleba, Y., Raskin, I. 1999. Production of recombinant proteins in plant root exudates. Nat. Biotechnol. 17:466-469.
11. Bouquin, T., Thomsen, M., Nielsen, L. K., Green, T. H., Mundy, J., Hanefeld Dziegiel, M. 2002. Human anti-rhesus D IgG1 antibody produced in transgenic plants. Transgenic Res. 11:115-122.
12. Bruyns, A. M., De Jaeger, G., De Neve, M., De Wilde, C., Van Montagu, M., Depicker, A. 1996. Bacterial and plant-produced scFv proteins have similar antigen-binding properties. FEBS Lett. 386:5-10.
13. Cabanes-Macheteau, M., Fitchette-Laine, A. C., Loutelier-Bourhis, C., Lange, C., Vine, N., Ma, J., Lerouge, P., Faye, L. 1999. N-Glycosylation of a mouse IgG expressed in transgenic tobacco plants. Glycobiology 9:365-372.
14. Canizares, M. C., Nicholson, L., Lomonossoff, G. P. 2005. Use of viral vectors for vaccine production in plants. Immunol. Cell. Biol. 83:263-270.
15. Chadd, H. E., Chamow, S. M. 2001. Therapeutic antibody expression technology. Curr. Opin. Biotrechnol. 12:188-194.
16. Chargelegue, D., Drake, P. M., Obregon, P., Prada, A., Fairweather, N., Ma, J. K. 2005. Highly immunogenic and protective recombinant vaccine candidate expressed in transgenic plants. Infect. Immun. 73:5915-5922.
17. Christensen, A. H., Quail, P. H. 1996. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res. 5:213-218.
18. Christou, P., Stoger, E., Twyman, R. M. 2004. Monocot systems for molecular farming. In: Fischer, R., Schillberg, S. (Eds.) Molecular Farming: Plant-made Pharmaceuticals and Technical Proteins. John Wiley & Sons Inc., New York, pp. 55-67.
19. Chu, L., Robinson, D. K. 2001. Industrial choices for protein production by large-scale cell culture. Curr. Opin. Biotechnol. 12:180-187.
20. Commandeur, U., Twyman, R. M., Fischer, R. 2003. The biosafety of molecular farming in plants. AgBiotechNet 5: ABN 110.
21. Conrad, U., Fiedler, U. 1998. Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Mol. Biol. 38:101-109.
22. CPMP. 2002. Points to consider on quality aspects of medicinal products containing active substances produced by stable transgene expression in higher plants (CPMP/BWP/764/02). The European Medicines Agency (EMEA), London.
23. CPMP. 2006. Guideline on the quality of biological active substances produced by stable transgene expression in higher plants. Draft 2. (EMEA/CHMP/BWP/48316/2006). The European Medicines Agency (EMEA), London.
24. Cramer, C. L., Boothe, J. G., Oishi, K. K. 1999. Transgenic plants for therapeutic proteins: linking upstream and downstream technologies. Curr. Top. Microbiol. Immunol. 240:95-118.
25. Daniell, H., Chebolu, S., Kumar, S., Singleton, M., Falconer, R. 2005a. Chloroplast-derived vaccine antigens and other therapeutic proteins. Vaccine 23:1779-1783.
26. Daniell, H., Kumar, S., Dufourmantel, N. 2005b. Breakthrough in chloroplast genetic engineering of agronomically important crops. Trends Biotechnol. 23:238-245.
27. D'Aoust, M. A., Lerouge, P., Busse, U., Bilodeau, P., Trepanier, S., Gomord, V., Faye, L., Vezina, L. P. 2004. Efficient and reliable production of pharmaceuticals in alfalfa. In: Fischer, R., Schillberg, S. (Eds.) Molecular Farming: Plant-made Pharmaceuticals and Technical Proteins. John Wiley & Sons, New York, pp. 1-12.
28. De Jaeger, G., Scheffer, S., Jacobs, A., Zambre, M., Zobell, O., Goossens, A., Depicker, A., Angenon, G. 2002. Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences. Nat. Biotechnol. 20:1265-1268.
29. De Marchis, F., Wang, Y., Stevanato, P., Arcioni, S., Bellucci, M. 2009. Genetic transformation of the sugar beet plastome. Transgenic Res. 18:17-30.
30. De Neve, M., De Loose, M., Jacobs, A., Van Houdt, H., Kaluza, B., Weidle, U., Van Montagu, M., Depicker, A. 1993. Assembly of an antibody and its derived antibody fragment in Nicotiana and Arabidopsis. Transgenic Res. 2:227-237.
31. De Wilde, C., De Rycke, R., Beeckman, T., De Neve, M., Van Montagu, M., Engler, G., Depicker, A. 1998. Accumulation pattern of IgG antibodies and Fab fragments in transgenic Arabidopsis thaliana plants. Plant Cell. Physiol. 39:639-646.
32. De Wilde, C., Peeters, K., Jacobs, A., Peck, I., Depicker, A. 2002. Expression of antibodies and Fab fragments in transgenic potato plants: a case study for bulk production in crop plants. Mol. Breeding 9:271-282.
33. Decker, E. L., Reski, R. 2004. The moss bioreactor. Curr. Opin. Plant Biol. 7:166-170.
34. Donson, J., Kearney, C. M., Hilf, M. E., Dawson, W. O. 1991. Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector. Proc. Natl Acad. Sci. USA 88:7204-7208.
35. Doran, P. M. 2006. Foreign protein degradation and instability in plants and plant tissue cultures. Trends Biotechnol. 24:426-432.
36. Drake, P. M., Chargelegue, D. M., Vine, N. D., van Dolleweerd, C. J., Obregon, P., Ma, J. K. 2003. Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots. Plant Mol. Biol. 52:233-241.
37. Drossard, J. 2003. Downstream processing of plant-derived recombinant therapeutic proteins. In: Fischer, R., Schillberg, S. (Eds.) Molecular Farming: Plant-made Pharmaceuticals and Technical Proteins. John Wiley & Sons Inc., New York, pp. 217-231.
38. Dyck, M. K., Lacroix, D., Pothier, F., Sirard, M. A. 2003. Making recombinant proteins in animals - different systems, different applications. Trends Biotechnol. 21:394-399.
39. Ehsani, P., Meunier, A., Nato, F., Jafari, A., Nato, A., Lafaye, P. 2003. Expression of anti human IL-4 and IL-6 scFvs in transgenic tobacco plants. Plant Mol. Biol. 52:17-29.
40. Fahrner, R. L., Knudsen, H. L., Basey, C. D., Galan, W., Feuerhelm, D., Vanderlaan, M., Blank, G. S. 2001. Industrial purification of pharmaceutical antibodies: development, operation, and validation of chromatography processes. Biotechnol. Genet. Eng. Rev. 18:301-327.
41. Faye, L., Boulaflous, A., Benchabane, M., Gomord, V., Michaud, D. 2005. Protein modifications in the plant secretory pathway: current status and practical implications in molecular pharming. Vaccine 23:1770-1778.
42. FDA. 2002. Guidance for industry. Drugs, biologics, and medical devices derived from bioengineered plants for use in humans and animals. Food and Drug Administration, Rockville MD.
43. Fischer, R., Emans, N. 2000. Molecular farming of pharmaceutical proteins. Transgenic Res. 9:279-299.
44. Fischer, R., Emans, N., Schuster, F., Hellwig, S., Drossard, J. 1999. Towards molecular farming in the future: using plant-cell-suspension cultures as bioreactors. Biotechnol. Appl. Biochem. 30:109-112.
45. Floss, D. M., Sack, M., Stadlmann, J., Rademacher, T., Scheller, J., Stöger, E., Fischer, R., Conrad, U. 2008. Biochemical and functional characterization of anti-HIV antibody-ELP fusion proteins from transgenic plants. Plant Biotechnol. J. 6:379-391.
46. Francisco, J. A., Gawlak, S. L., Miller, M., Bathe, J., Russell, D., Chace, D., Mixan, B., Zhao, L., Fell, H. P., Siegall, C. B. 1997. Expression and characterization of bryodin 1 and a bryodin 1-based single-chain immunotoxin from tobacco cell culture. Bioconjug. Chem. 8:708-713.
47. Franconi, R., Roggero, P., Pirazzi, P., Arias, F. J., Desiderio, A., Bitti, O., Pashkoulov, D., Mattei, B., Bracci, L., Masenga, V., Milne, R. G., Benvenuto, E. 1999. Functional expression in bacteria and plants of an scFv antibody fragment against tospoviruses. Immunotechnology 4:189-201.
48. Franklin, S. E., Mayfield, S. P. 2005. Recent developments in the production of human therapeutic proteins in eukaryotic algae. Expert Opin. Biol. Ther. 5:225-235.
49. Gasdaska, J. R., Spencer, D., Dickey, L. 2003. Advantages of therapeutic protein production in the aquatic plant Lemna. BioProcessing J. Mar/Apr: 49-56.
50. Gavilondo, J. V., Larrick, J. W. 2000. Antibody production technology in the millennium. Biotechniques 29:128-145.
51. Gerngross, T. U. 2004. Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nat. Biotechnol. 22:1409-1414.
52. Gleba, Y., Klimyuk, V., Marillonnet, S. 2005. Magnifection - a new platform for expressing recombinant vaccines in plants. Vaccine 23:2042-2048.
53. Gleba, Y., Marillonnet, S., Klimyuk, V. 2004. Engineering viral expression vectors for plants: the 'full virus' and the 'deconstructed virus' strategies. Curr. Opin. Plant Biol. 7:182-188.
54. Gomord, V., Chamberlain, P., Jefferis, R., Faye, L. 2005. Biopharmaceutical production in plants: problems, solutions and opportunities. Trends Biotechnol. 23:559-565.
55. Gomord, V., Sourrouille, C., Fitchette, A. C., Bardor, M., Pagny, S., Lerouge, P., Faye, L. 2004. Production and glycosylation of plant-made pharmaceuticals: the antibodies as a challenge. Plant Biotechnol. J. 2:83-100.
56. Green, L. 1999. Antibody engineering via genetic engineering of the mouse: xenomouse strains are a vehicle for the facile generation of therapeutic human monoclonal antibodies. J. Immunol. Methods 231:11-23.
57. Griffiths, A., Duncan, A. 1998. Strategies for selection of antibodies by phage display. Curr. Opin. Biotechnol. 9:102-108.
58. Harvey, A. J., Speksnijder, G., Baugh, L. R., Morris, J. A., Ivarie, R. 2002. Expression of exogenous protein in the egg white of transgenic chickens. Nat. Biotechnol. 20:396-399.
59. Hellwig, S., Drossard, J., Twyman, R. M., Fischer, R. 2004. Plant cell cultures for the production of recombinant proteins. Nat. Biotechnol. 22:1415-1422.
60. Hendy, S., Chen, Z. C., Barker, H., Santa Cruz, S., Chapman, S., Torrance, L., Cockburn, W., Whitelam, G. C. 1999. Rapid production of single-chain Fv fragments in plants using a potato virus X episomal vector. J. Immunol. Methods 231:137-146.
61. Hiatt, A., Cafferkey, R., Bowdish, K. 1989. Production of antibodies in transgenic plants. Nature 342:76-78.
62. Hood, E. E., Woodard, S. L., Horn, M. E. 2002. Monoclonal antibody manufacturing in transgenic plants - myths and realities. Curr. Opin. Biotechnol. 13:630-635.
63. Hull, A. K., Criscuolo, C. J., Mett, V., Groen, H., Steeman, W., Westra, H., Chapman, G., Legutki, B., Baillie, L., Yusibov, V. 2005. Human-derived, plant-produced monoclonal antibody for the treatment of anthrax. Vaccine 23:2082-2086.
64. Ikonomou, L., Schneider, Y. J., Agathos, S. N. 2003. Insect cell culture for industrial production of recombinant proteins. Appl. Microbiol. Biotechnol. 62:1-20.
65. Jefferis, R. 2001. Glycosylation of human IgG antibodies: relevance to therapeutic applications. Biopharm 14:19-27.
66. Kapila, J., De Rycke, R., van Montagu, M., Angenon, G. 1997. An agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci. 122:101-108.
67. Kathuria, S., Sriraman, R., Nath, R., Sack, M., Pal, R., Artsaenko, O., Talwar, G. P., Fischer, R., Finnern, R. 2002. Efficacy of plant-produced recombinant antibodies against HCG. Hum. Reprod. 17:2054-2061.
68. Khoudi, H., Laberge, S., Ferullo, J. M., Bazin, R., Darveau, A., Castonguay, Y., Allard, G., Lemieux, R., Vezina, L. P. 1999. Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol. Bioeng. 64:135-143.
69. Kipriyanov, S. M., Little, M. 1999. Generation of recombinant antibodies. Mol. Biotechnol. 12:173-201.
70. Ko, K., Steplewski, Z., Glogowska, M., Koprowski, H. 2005. Inhibition of tumor growth by plantderived mAb. Proc. Natl. Acad. Sci. USA 102:7026-7030.
71. Ko, K., Tekoah, Y., Rudd, P. M., Harvey, D. J., Dwek, R. A., Spitsin, S., Hanlon, C. A., Rupprecht, C., Dietzschold, B., Golovkin, M., Koprowski, H. 2003. Function and glycosylation of plantderived antiviral monoclonal antibody. Proc. Natl. Acad. Sci. USA 100:8013-8018.
72. Komarnytsky, S., Borisjuk, N. V., Borisjuk, L. G., Alam, M. Z., Raskin, I. 2000. Production of recombinant proteins in tobacco guttation fluid. Plant Physiol. 124:927-933.
73. Lelivelt, C. L., McCabe, M. S., Newell, C. A., Desnoo, C. B., van Dun, K. M., Birch-Machin, I., Gray, J. C., Mills, K. H., Nugent, J. M. 2005. Stable plastid transformation in lettuce (Lactuca sativa L.). Plant Mol. Biol. 58:763-774.
74. Ma, J. K., Drake, P. M., Christou, P. 2003. The production of recombinant pharmaceutical proteins in plants. Nat. Rev. Genet. 4:794-805.
75. Ma, J. K., Hiatt, A., Hein, M., Vine, N. D., Wang, F., Stabila, P., van Dolleweerd, C., Mostov, K., Lehner, T. 1995. Generation and assembly of secretory antibodies in plants. Science 268:716-719.
76. Magee, A. M., Coyne, S., Murphy, D., Horvath, E. M., Medgyesy, P., Kavanagh, T. A. 2004. T7 RNA polymerase-directed expression of an antibody fragment transgene in plastids causes a semi-lethal pale-green seedling phenotype. Transgenic Res. 13:325-337.
77. Makvandi-Nejad, S., McLean, M. D., Hirama, T., Almquist, K. C., Mackenzie, C. R., Hall, J. C. 2005. Transgenic tobacco plants expressing a dimeric single-chain variable fragment (scFv) antibody against Salmonella enterica serotype Paratyphi B. Transgenic Res. 14:785-792.
78. Maliga, P. 2003. Progress towards commercialization of plastid transformation technology. Trends Biotechnol. 21:20-28.
79. Maliga, P. 2004. Plastid transformation in higher plants. Annu. Rev. Plant Biol. 55:289-313.
80. Marillonnet, S., Thoeringer, C., Kandzia, R., Klimyuk, V., Gleba, Y. 2005. Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat. Biotechnol. 23:718-723.
81. Mayfield, S. P., Franklin, S. E. 2005. Expression of human antibodies in eukaryotic micro-algae. Vaccine 23:1828-1832.
82. Mayfield, S. P., Franklin, S. E., Lerner, R. A. 2003. Expression and assembly of a fully active antibody in algae. Proc. Natl. Acad. Sci. USA 100:438-442.
83. McCormick, A. A., Kumagai, M. H., Hanley, K., Turpen, T. H., Hakim, I., Grill, L. K., Tuse, D., Levy, S., Levy, R. 1999. Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proc. Natl. Acad. Sci. USA 96:703-708.
84. Menkhaus, T. J., Bai, Y., Zhang, C., Nikolov, Z. L., Glatz, C. E. 2004. Considerations for the recovery of recombinant proteins from plants. Biotechnol. Prog. 20:1001-1014.
85. Menkhaus, T. J., Glatz, C. E. 2005. Antibody capture from corn endosperm extracts by packed bed and expanded bed adsorption. Biotechnol. Prog. 21:473-485.
86. Nikolov, Z. L., Regan, J. T., Dickey, L. F., Woodard, S. L. 2009. Purification of antibodies from transgenic plants. In: Gottschalk, U. (Ed.) Process Scale Purification of Antibodies. John Wiley & Sons, New York, pp. 387-406.
87. Nikolov, Z. L., Woodard, S. L. 2004. Downstream processing of recombinant proteins from transgenic feedstock. Curr. Opin. Biotechnol. 15:479-486.
88. Nugent, G. D., Coyne, S., Nguyen, T. T., Kavanagh, T. A., Dix, P. J. 2006. Nuclear and plastid transformation of Brassica oleracea var. botrytis (cauliflower) using PEG-mediated uptake of DNA into protoplasts. Plant Sci. 170:135-142.
89. Padidam, M. 2003. Chemically regulated gene expression in plants. Curr. Opin. Plant Biol. 6:169-177.
90. Perrin, Y., Vaquero, C., Gerrard, I., Sack, M., Drossard, J., Stoger, E., Christou, P., Fischer, R. 2000. Transgenic pea seeds as bioreactors for the production of a single-chain Fv fragment (scFv) antibody used in cancer diagnosis and therapy. Mol. Breeding 6:345-352.
91. Platis, D., Drossard, J., Fischer, R., Ma, J. K., Labrou, N. E. 2008. New downstream processing strategy for the purification of monoclonal antibodies from transgenic tobacco plants. J. Chromatogr. A. 1211:80-89.
92. Rademacher, T., Sack, M., Arcalis, E., Stadlmann, J., Balzer, S., Altmann, F., Quendler, H., Stiegler, G., Kunert, R., Fischer, R., Stoger, E. 2008. Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans. Plant Biotechnol. J. 6:189-201.
93. Raju, T. S., Briggs, J., Borge, S. M., Jones, AJ. S. 2000. Species-specific variation in glycosylation of IgG: evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics. Glycobiolgy 10:477-486.
94. Ramessar, K., Capell, T., Christou, P. 2008a. Molecular pharming in cereal crops. Phytochem. Rev. 7:579-592.
95. Ramessar, K., Capell, T., Twyman, R. M., Quemada, H., Christou, P. 2008b. Trace and traceabilitya call for regulatory harmony. Nat. Biotechnol. 26:975-978.
96. Ramessar, K., Rademacher, T., Sack, M., Stadlmann, J., Platis, D., Stiegler, G, Labrou, N., Altmann, F., Ma, J., Stöger, E., Capell, T., Christou, P. 2008c. Cost-effective production of a vaginal protein microbicide to prevent HIV transmission. Proc. Natl. Acad. Sci. USA 105:3727-3732.
97. Ramírez, N, Ayala, M., Lorenzo, D., Palenzuela, D., Herrera, L., Doreste, V., Pérez, M., Gavilond, J. V., Oramas, P. 2002. Expression of a single-chain Fv antibody fragment specific for the hepatitis B surface antigen in transgenic tobacco plants. Transgenic Res. 11:61-64.
98. Rodriguez, M., Ramirez, N. I., Ayala, M., Freyre, F., Perez, L., Triguero, A., Mateo, C, Selman-Housein, G., Gavilondo, J. V., Pujol, M. 2005. Transient expression in tobacco leaves of an aglycosylated recombinant antibody against the epidermal growth factor receptor. Biotechnol. Bioeng. 89:188-194.
99. Sack, M., Paetz, A., Kunert, R., Bomble, M., Hesse, F., Stiegler, G, Fischer, R., Katinger, H, Stoeger, E., Rademacher, T. 2007. Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures. FASEB J. 21:1655-1664.
100. Schaefer, D. G 2002. A new moss genetics: targeted mutagenesis in Physcomitrellapatens. Annu. Rev. Plant Biol. 53:477-501.
101. Schillberg, S., Emans, N, Fischer, R. 2002. Antibody molecular farming in plants and plant cells. Phytochem. Rev. 1:45-54.
102. Schillberg, S., Fischer, R., Emans, N. 2003. Molecular farming of recombinant antibodies in plants. Cell. Mol. Life Sci. 60:433-445.
103. Schillberg, S., Zimmermann, S., Voss, A., Fischer, R. 1999. Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum. Transgenic Res. 8:255-263.
104. Schillberg, S., Zimmermann, S., Zhang, M. Y., Fischer, R. 2001. Antibody-based resistance to plant pathogens. Transgenic Res. 10:1-12.
105. Schunmann, PH. D., Coia, G, Waterhouse, P. M. 2002. Biopharming the SimpliREDTM HIV diagnostic reagent in barley, potato and tobacco. Mol. Breeding 9:113-121.
106. Schunmann, PH. D., Surin, B., Waterhouse, P. M. 2003. A suite of novel promoters and terminators for plant biotechnology. II. The pPLEX series for use in monocots. Funct. Plant Biol. 30:453-460.
107. Semenyuk, E. G., Orlova, I. V., Stremovskii, O. A., Balandin, T. G., Nosov, A. M., Bur'yanov, Y, Deev, S. M. 2002. Transgenic tobacco plants produce miniantibodies against human ferritin. Dokl. Biochem. Biophys. 384:176-178.
108. Seveno, M., Bardor, M., Paccalet, T, Gomord, V, Lerouge, P., Faye, L. 2004. Glycoprotein sialylation in plants? Nat. Biotechnol. 22:1351-1352.
109. Shah, M. M., Fujiyama, K., Flynn, C. R., Joshi, L. 2003. Sialylated endogenous glycoconjugates in plant cells. Nat. Biotechnol. 21:1470-1471.
110. Shah, M. M., Fujiyama, K., Flynn, C. R., Joshi, L. 2004. Glycoprotein sialylation in plants? Reply. Nat. Biotechnol. 22:1352-1353.
111. Sharp, J. M., Doran, P. M. 2001a. Characterization of monoclonal antibody fragments produced by plant cells. Biotechnol. Bioeng. 73:338-346.
112. Sharp, J. M., Doran, P. M. 2001b. Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures. Biotechnol. Prog. 17:979-992.
113. Sidhu, S. S. 2000. Phage display in pharmaceutical biotechnology. Curr. Opin. Biotechnol. 11:610-616.
114. Sparrow, P. A. C., Irwin, J. A., Dale, P. J., Twyman, R. M. and Ma, J. K. C. 2007. Pharma-Planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals. Transgenic Res. 16:147-161.
115. Spök, A., Twyman, R. M., Fischer, R., Ma, J. K. C., Sparrow, P. A. C. 2008. Evolution of a regulatory framework for plant-made pharmaceuticals. Trends Biotechnol. 26:506-517.
116. Sriraman, R., Bardor, M., Sack, M., Vaquero, C., Faye, L., Fischer, R., Finnern, R., Lerouge, P. 2004. Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core-a (1, 3)-fucose residues. Plant Biotechnol. J. 2:279-287.
117. Sriraman, R., Sack, M., Talwar, G. P., Fischer, R. 2003. Glycosylation of recombinant antibodies in plants. In: Proceedings of the Ninth Annual Ranbaxy Science Foundation Symposium. Ranbaxy Science Foundation, New Delhi, pp. 89-98.
118. Stoger, E., Ma, J. K., Fischer, R., Christou, P. 2005a. Sowing the seeds of success: pharmaceutical proteins from plants. Curr. Opin. Biotechnol. 16:167-173.
119. Stoger, E., Sack, M., Nicholson, L., Fischer, R., Christou, P. 2005b. Recent progress in plantibody technology. Curr. Pharm. Des. 11:2439-2457.
120. Stoger, E., Sack, M., Perrin, Y., Vaquero, C., Torres, E., Twyman, R. M., Christou, P., Fischer, R. 2002. Practical considerations for pharmaceutical antibody production in different crop systems. Mol. Breeding 9:149-158.
121. Stoger, E., Vaquero, C., Torres, E., Sack, M., Nicholson, L., Drossard, J., Williams, S., Keen, D., Perrin, Y., Christou, P., Fischer, R. 2000. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol. Biol. 42:583-590.
122. Strasser, R., Altmann, F., Mach, L., Glossl, J., Steinkellner, H. 2004. Generation of Arabidopsis thaliana plants with complex N-glycans lacking beta1, 2-linked xylose and core alpha1, 3-linked fucose. FEBS Lett. 561:132-136.
123. Subroto, M. A., Hamill, J. D., Doran, P. M. 1996. Development of shooty teratomas from several solanaceous plants: growth kinetics, stoichiometry and alkaloid production. J. Biotechnol. 45:45-57.
124. Torres, E., Vaquero, C., Nicholson, L., Sack, M., Stoger, E., Drossard, J., Christou, P., Fischer, R., Perrin, Y. 1999. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies. Transgenic Res. 8:441-449.
125. Triguero, A., Cabrera, G., Cremata, J. A., Yuen, C. T., Wheeler, J., Ramírez, N. I. 2005. Plant-derived mouse IgG monoclonal antibody fused to KDEL endoplasmic reticulum-retention signal is N-glycosylated homogeneously throughout the plant with mostly high-mannose-type N-glycans. Plant Biotechnol. J. 3:449-457.
126. Twyman, R. M., Schillberg, S., Fischer, R. 2005. Transgenic plants in the biopharmaceutical market. Expert Opin. Emerg. Drugs 10:185-218.
127. Twyman, R. M., Stoger, E., Schillberg, S., Christou, P., Fischer, R. 2003. Molecular farming in plants: host systems and expression technology. Trends Biotechnol. 21:570-578.
128. Valdes, R., Gomez, L., Padilla, S., Brito, J., Reyes, B., Alvarez, T., Mendoza, O., Herrera, O., Ferro, W., Pujol, M., Leal, V., Linares, M., Hevia, Y., Garcia, C., Mila, L., Garcia, O., Sanchez, R., Acosta, A., Geada, D., Paez, R., Luis Vega, J., Borroto, C. 2003a. Large-scale purification of an antibody directed against hepatitis B surface antigen from transgenic tobacco plants. Biochem. Biophys. Res. Commun. 308:94-100.
129. Valdes, R., Reyes, B., Alvarez, T., Garcia, J., Montero, J. A., Figueroa, A., Gomez, L., Padilla, S., Geada, D., Abrahantes, M. C., Dorta, L., Fernandez, D., Mendoza, O., Ramirez, N., Rodriguez, M., Pujol, M., Borroto, C., Brito, J. 2003b. Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale. Biochem. Biophys. Res. Commun. 310:742-747.
130. Van Droogenbroeck, B., Cao, J., Stadlmann, J., Altmann, F., Colanesi, S., Hillmer, S., Robinson, D. G., Van Lerberge, E., Terryn, N., Van Montagu, M., Liang, M., Depicker, A., De Jaeger, G. 2007. Aberrant localization and underglycosylation of highly accumulating single-chain Fv-Fc antibodies in transgenic Arabidopsisseeds. Proc. Natl. Acad. Sci. USA 104:1430-1435.
131. Vaquero, C., Sack, M., Chandler, J., Drossard, J., Schuster, F., Monecke, M., Schillberg, S., Fischer, R. 1999. Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc. Natl. Acad. Sci. USA 96:11128-11133.
132. Vaquero, C., Sack, M., Schuster, F., Finnern, R., Drossard, J., Schumann, D., Reimann, A., Fischer, R. 2002. A carcinoembryonic antigen-specific diabody produced in tobacco. FASEB J. 16:408-410.
133. Verch, T., Yusibov, V., Koprowski, H. 1998. Expression and assembly of a full-length monoclonal antibody in plants using a plant virus vector. J. Immunol. Methods 220:69-75.
134. Vine, N. D., Drake, P., Hiatt, A., Ma, J. K. 2001. Assembly and plasma membrane targeting of recombinant immunoglobulin chains in plants with a murine immunoglobulin transmembrane sequence. Plant Mol. Biol. 45:159-167.
135. Warner, T. G. 2000. Metabolic engineering glycosylation: biotechnology's challenge to the glycobiologist in the new millennium. In: Ernst, B., Hart, G. W., Sanay, P. (Eds.) Carbohydrates in Chemistry and Biology. Wiley-VCH, New York, pp. 1043-1064.
136. Wurm, F. M. 2004. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22:1393-1398.
137. Yano, A., Maeda, F., Takekoshi, M. 2004. Transgenic tobacco cells producing the human monoclonal antibody to hepatitis B virus surface antigen. J. Med. Virol. 73:208-215.
138. Yusibov, V., Rabindran, S. 2008. Recent progress in the development of plant derived vaccines. Expert Rev. Vaccines 7:1173-1183.
139. Yusibov, V., Rabindran, S., Commandeur, U., Twyman, R. M., Fischer, R. 2006. The potential of plant virus vectors for vaccine production. Drugs R&D 7:203-217.
140. Zeitlin, L., Olmsted, S. S., Moench, T. R., Co, M. S., Martinell, B. J., Paradkar, V. M., Russell, D. R., Queen, C., Cone, R. A., Whaley, K. J. 1998. A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes. Nat. Biotechnol. 16:1361-1364.
141. Ziegler, A., Cowan, G. H., Torrance, L., Ross, H. A., Davies, H. V. 2000. Facile assessment of cDNA constructs for expression of functional antibodies in plants using the potato virus X vector. Mol. Breeding 6:327-335.
142. Zimmermann, S., Schillberg, S., Liao, Y. C., Fisher, R. 1998. Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum. Mol. Breeding. 4:369-379.