Antibody molecular farming in plants and plant cells

Phytochemistry Reviews

Volumn 1, Issue 1, 2002, Pages 45-54

  Schillberg, Stefan ^a   Emans, Neil ^b   Fischer, Rainer ^a,b  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Molecular Farming; Recombinant antibody; Recombinant protein; Transgenic plant

Indexed keywords

ENZYME; GROWTH FACTOR; PLASMA PROTEIN; RECOMBINANT ANTIBODY; VACCINE;

AGRICULTURAL PROCEDURES; ANTIBODY ENGINEERING; ANTIBODY PRODUCTION; CELL TRANSFORMATION; CULTURE OPTIMIZATION; DRUG MANUFACTURE; DRUG SAFETY; DRUG SYNTHESIS; DRUG UTILIZATION; MOLECULAR BIOLOGY; MOLECULAR FARMING; NONHUMAN; PLANT BIOTECHNOLOGY; PLANT CELL CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PROTEIN TARGETING; REVIEW; SUSPENSION CELL CULTURE; TRANSGENIC PLANT;

ANIMALIA;

EID: 0012515015     PISSN: 15687767     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015880218651     Document Type: Review

References (87)

1. An G (1985) High efficiency transformation of cultured tobacco cells. Plant Physiol. 79: 568-570.
2. Archambault J (1991) Large-scale (20 1) culture of surface-immobilized Catharanthus roseus cells. Enzyme Microb. Technol. 13: 882-892.
3. Artsaenko O, Kettig B, Fiedler U, Conrad U & Düring K (1998) Potato tubers as a biofactory for recombinant antibodies. Mol. Breed. 4: 313-319.
4. Artsaenko O, Peisker M, zur Nieden U, Fiedler U, Weiler EW, Müntz K & Conrad U (1995) Expression of a single-chain Fv antibody against abscisic acid creates a wilty phenotype in transgenic tobacco. Plant J. 8: 745-750.
5. Bakker H, Bardor M, Molthoff J, Gormord V, Elbers I, Stevens L, Jordi W, Lommen A, Faye L, Lerouge P & Bosch D (2001) Galactose-extended glycans of antibodies produced by transgenic plants. Proc. Natl. Acad. Sci. USA 98: 2899-2904.
6. Baum TJ, Hiatt A, Parrott WA, Pratt LH & Hussey RS (1996) Expression in tobacco of a functional monoclonal antibody specific to stylet secretions of the root-knot nematode. MPMI 9: 382-387.
7. Bisaria V & Panda A ( 1991) Large-scale plant cell culture: Methods, applicatons and products. Curr. Opin. Biotechnol. 2: 370-374.
8. Burton D (1995) Phage display. Immunotechnology 1: 87-94.
9. Carpita N, Sabularse D, Montezinos D & Delmer DP (1979) Determination of the pore size of cell walls of living plant cells. Science 205: 1144-1147.
10. Chargelegue D, Vine N, van Dolleweerd C, PMW D & Ma J (2000) A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice. Transgenic Res. 9: 187-194.
11. Chen MH, Liu LF, Chen YR, Wu HK & Yu SM (1994) Expression of alpha-amylases, carbohydrate metabolism, and autophagy in cultured rice cells is coordinately regulated by sugar nutrient. Plant J. 6: 625-636.
12. Christou P (1993) Particle gun-mediated transformation. Curr. Opin. Biotechnol. 4: 135-141.
13. Christou P (1995) Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment. Euphytica 85: 13-27.
14. Conrad U & Fiedler U (1998) Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Mol. Biol. 38: 101-109.
15. Daniell T & Edwards R (1995) Changes in protein methylation associated with the elicitation response in cell cultures of alfalfa (Medicago sativa L.). FEBS Lett. 360: 57-61.
16. De Jaeger G, Buys E, Eeckhout D, De Wilde C, Jacobs A, Kapila J, Angenon G, Van Montagu M, Gerats T & Depicker A (1998) High level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida. Eur. J. Biochem. 259: 1-10.
17. De Neve M, De Loose M, Jacobs A, Van Houdt H, Kaluza B, Weidle U, Van Montagu M & Depicker A (1993) Assembly of an antibody and its derived antibody fragment in Nicotiana and Arabidopsis. Transgenic Res. 2: 227-237.
18. De Wilde C, De Neve M, De Rycke R, Bruyns AM, De Jaeger G, Van Montagu M, Depicker A & Engler G (1996) Intact antigen-binding MAK33 antibody and Fab fragment accumulate in intercellular spaces of Arabidopsis thaliana. Plant Sci. 114: 233-241.
19. Desikan R, Hancock JT, Neill SJ, Coffey MJ & Jones OT (1996) Elicitor-induced generation of active oxygen in suspension cultures of Arabidopsis thaliana. Biochem. Soc. Trans. 24: 199S.
20. During K, Hippe S, Kreuzaler F & Schell J (1990) Synthesis and selfassembly of a functional monoclonal antibody in transgenic Nicotiana tabacum. Plant Mol. Biol. 15: 281-293.
21. Fecker LF, Kaufmann A, Commandeur U, Commandeur J, Koenig R & Burgermeister W (1996) Expression of single-chain antibody fragments (scFv) specific for beet necrotic yellow vein virus coat protein or 25kDa protein in Escherichia coli and Nicotiana benthamiana. Plant Mol. Biol. 32: 979-986.
22. Fiedler U & Conrad U (1995) High-level production and long-term storage of engineered antibodies in transgenic tobacco seeds. Bio/Technology 13: 1090-1093.
23. Fiedler U, Philips J, Artsaenko O & Conrad U (1997) Optimisation of scFv antibody production in transgenic plants. Immunotechnology 3: 205-216.
24. Firek S, Draper J, Owen MR, Gandecha A, Cockburn B & Whitelam GC (1993) Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures [published erratum appeal's in Plant Mol Biol (1994) 24(5): 833]. Plant Mol. Biol. 23: 861-870.
25. Fischer R, Drossard J, Hellwig S, Emans N & Schillberg S (1998a) Transgenic plants as bioreactors for the expression of recombinant antibodies. In: Talwar N (ed) Proceedings of the X international congress of immunology (pp. 307-313). Monduzzi Editore Publishers, Bologna.
26. Fischer R, Drossard J, Liao YC & Schillberg S (1998b) Characterisation and applications of plant-derived recombinant antibodies. In: Cunningham C & Porter AJR (eds) Methods in biotechnology, Vol. 3: Recombinant proteins in plants: Production and isolation of clinically useful compounds (pp. 129-142). Humana Press, Totowa, NJ.
27. Fischer R, Emans N, Schuster F, Hellwig S & Drossard J (1999a) Towards molecular farming in the future: Using plant cell suspension cultures as bioreactors. Biotechnol. Appl. Biochem. 30: 113-116.
28. Fischer R, Liao Y-C & Drossard J (1999b) Affinity-purification of a TMV-specific recombinant full-size antibody from a transgenic tobacco suspension culture. J. Immunol. Meth. 226: 1-10.
29. Fischer R, Schumann D, Zimmermann S, Drossard J, Sack M & Schillberg S (1999c) Expression and characterization of bispecific single chain Fv fragments produced in transgenic plants. Eur. J. Biochem. 262: 810-816.
30. Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry JS, Galluppi GR, Goldberg SB, Hoffmann NL & Woo SC (1983) Expression of bacterial genes in plant cells. Proc. Natl. Acad. Sci. USA 80: 4803-4807.
31. Hiatt A, Cafferkey R & Bowdish K (1989) Production of antibodies in transgenic plants. Nature 342: 76-78.
32. Hilton MG & Rhodes MJ (1990) Growth and hyoscyamine production of 'hairy root' cultures of Datura stramonium in a modified stirred tank reactor. Appl. Microbiol. Biotechnol. 33: 132-138.
33. Hoehl U, Upmeier B & Barz W (1988) Growth and nicotinate biotransformation in batch cultured and airlift fermenter grown soybean cell suspension cultures. Appl. Microbiol. Biotechnol. 28: 319-323.
34. Hogue RS, Lee JM & An G (1990) Production of a foreign protein product with genetically modified plant cells. Enzyme Microb. Technol. 12: 533-538.
35. Hood E, Witcher D, Maddock S, Meyer T, Baszczynski C, Bailey M, Flynn P, Register J, Marshall L, Bond D, Kulisek E, Kusnadi A, Evangelista R, Nikolov Z, Wooge C, Mehigh R, Hernan R, Kappel W, Ritland D, Li C-P & Howard J (1997) Commercial production of Avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Mol. Breed. 3: 291-306.
36. Hood EE, Kusnadi A, Nikolov C & Howard JA (1999) Molecular farming of industrial proteins from transgenic maize. Adv. Exp. Med. Biol. 464: 127-147.
37. Hooker BS, Lee JM & An G (1990) Cultivation of plant cells in a stirred tank reactor. Biotechnol. Bioeng. 35: 296-304.
38. Horsch R, Fry JE, Hoffman N, Eicholtz D, Rogers S & Fraley R (1985) A simple and general method for transferring genes into plants. Science 227: 1229-1231.
39. Kapila J, De Rycke R, van Montagu M & Angenon G (1996) An Agrobacterium mediated transient gene expression system for intact leaves. Plant Sci. 122: 101-108.
40. Kieran PM, MacLoughlin PF & Malone DM (1997) Plant cell suspension cultures: some engineering considerations. J. Biotechnol. 59: 39-52.
41. Koehler G & Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497.
42. L-DNA gene 5 controls the tissue specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector. Mol. Gen. Genet. 204: 383-396.
43. Kusnadi AR, Nikolov ZL & Howard JA (1997) Production of recombinant proteins in transgenic plants: Practical considerations. Biotechnol. Bioeng. 56: 473-184.
44. Larrick JW, Yu L, Chen J, Jaiswal S & Wycoff K ( 1998) Production of antibodies in transgenic plants. Res. Immunol. 149: 603-608.
45. Leite A, Kemper E, da Silva M, Luchessi A, Siloto R, Bonaccorsi E, El-Dorry H & Arruda P (2000) Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants. Mol. Breed. 6: 47-53.
46. Lindsey K & Jones MGK ( 1987) Transient gene expression in electroporated protoplasts and intact cells of sugar beet. Plant Mol. Biol. 10: 43-52.
47. Longstaff M, Newell CA, Boonstra B, Strachan G, Learmonth D, Harris WJ, Porter AJ & Hamilton WD (1998) Expression and characterisation of single-chain antibody fragments produced in transgenic plants against the organic herbicides atrazine and paraquat. Biochim. Biophys. Acta 1381: 147-160.
48. Ma J & Hein M (1995) Plant antibodies for immunotherapy. Plant Physiol. 109: 341-346.
49. Ma JK, Hiatt A, Hein M, Vine ND, Wang F, Stabila P, van Dolleweerd C, Mostov K & Lehner T ( 1995) Generation and assembly of secretory antibodies in plants. Science 268: 716-719.
50. Ma JK, Hikmat BY, Wycoff K, Vine ND, Chargelegue D, Yu L, Hein MB & Lehner T ( 1998) Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nat. Med. 4: 601-606.
51. Ma JKC, Lehner T, Stabila P, Fux CI & Hiatt A (1994) Assembly of monoclonal antibodies with IgG 1 and IgA heavy chain domains in transgenic tobacco plants. Eur. J. Immunol. 24: 131-138.
52. Magnuson NS, Linzmaier PM, Reeves R, An G, HayGlass K & Lee JM (1998) Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. Prot. Expr. Purif. 13: 45-52.
53. Mason HS & Arntzen CJ (1995) Transgenic plants as vaccine production systems. Trends Biotechnol. 13: 388-392.
54. McCormick AA, Kumagai MH, Hanley K, Turpen TH, Hakim I, Grill LK, Tusé D, Levy S & Levy R (1999) Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proc. Natl. Acad. Sci. USA 96: 703-708.
55. Moloney MM & Holbrook LA (1997) Subcellular targeting and purification of recombinant proteins in plant production systems. Biotechnol. Genet. Eng. Rev. 14: 321-336.
56. Nagata T, Nemoto Y & Seiichiro H (1992) Tobacco BY-2 cell line as the 'HeLa' cell in the cell biology of higher plants. Int. Rev. Cytol. 132: 1-30.
57. Owen M, Gandecha A, Cockburn B & Whitelam G (1992) Synthesis of a functional anti-phytochrome single-chain Fv protein in transgenic tobacco. Bio/Technology 10: 790-794.
58. Phillips J, Artsaencko O, Fiedler U, Horstmann C, Mock HP, Müntz K & Conrad U (1997) Seed-specific immunomodulation of abscisic acid activity induces a developmental switch. EMBO J. 16: 4489-1496.
59. Porta C & Lomonossoff GP (1996) Use of viral replicons for the expression of genes in plants. Mol. Biotech. 5: 209-221.
60. Russell D & Fuller J (2000) Method for producing antibodies in plant cells. Monsanto Company, St. Louis, Mo., USA.
61. Schillberg S, Zimmermann S, Findlay K & Fischer R (2000) Plasma membrane display of anti-viral single chain Fv fragments confers resistance to tobacco mosaic virus. Mol. Breed. 6: 317-326.
62. Schillberg S, Zimmermann S, Voss A & Fischer R (1999) Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum. Transgenic Res. 8: 255-263.
63. Schillberg S, Zimmermann S, Zhang MY & Fischer R (2001) Antibody-based resistance to plant pathogens. Transgenic Res. 10: 1-12.
64. Schlatmann JE, ten Hoopen HJG & Heijnen JJ (1996) Large-scale production of secondary metabolites by plant cell cultures. In: DiCosmo F & Misawa M (ed) Plant cell culture secondary metabolism: Toward industrial application. CRC Press, Boca Raton.
65. Scheuten A, Roosien J, de Boer JM, Wilmink A, Rosso MN, Bosch D, Stiekema WJ, Gommers FJ, Bakker J & Schots A (1997) Improving scFv antibody expression levels in the plant cytosol. FEBS Lett. 415:235-241.
66. Schouten A, Roosien J, van Engelen FA, de long GAM, Borst-Vrenssen AWM, Zilverentant JF, Bosch D, Stiekema WJ, Gommers FJ, Schots A & Bakker J. (1996) The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both cytosol and the secretory pathway in transgenic tobacco. Plant Mol. Biol. 30: 781-793.
67. Seki M, Ohzora C, Takeda M & Furusaki S (1997) Taxol (Paclitaxel) production using free and immobilized cells of Taxus cuspidata. Biotechnol. Bioeng. 53: 214-219.
68. Sijmons PC, Dekker BMM, Schrammeijer B, Verwoerd TC, van den Elzen PJM & Hoekema A (1990) Production of correctly processed human serum albumin in transgenic plants. Bio/Technology 8: 217-221.
69. Spiegel H, Schillberg S, Sack M, Holzem A, Nähring J, Monecke M, Liao Y-C & Fischer R (1999) Expression of antibody fusion proteins in the cytoplasm and ER of plant cells. Plant Sci. 149: 63-71.
70. Staub J, Garcia B, Graves J, Hajdukiewicz P, Hunter P, Nehra N, Paradkar V, Schlittler M, Carroll J, Spatola L, Ward D, Ye G & Russell D (2000) High-yield production of a human therapeutic protein in tobacco chloroplasts. Nature Biotechnol. 18: 333-338.
71. Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A & Galeffi P (1993) Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366: 469-472.
72. ten Hoopen HJG, van Gulik WM & Heijnen JJ (1992) Continuous culture of suspended plant cells. In vitro Cell. Dev. Biol. 28: 115-120.
73. Terashima M, Murai Y, Kawamura M, Nakanishi S, Stoltz T, Chen L, Drohan W, Rodriguez RL & Katoh S (1999) Production of functional human alpha 1-antitrypsin by plant cell culture. Appl. Microbiol. Biotechnol. 52: 516-523.
74. Torres E, Vaquero C, Nicholson L, Sack M, Stöger E, Drossard J, Christou P & Fischer R (1999) Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies. Transgen. Res. 8: 441-449.
75. Van Der Heijden R, Verpoorte R & Ten Hoopen HJG (1989) Cell and tissue cultures of Catharanthus roseus L. G. Don - a Literature Survey. Plant Cell Tiss. Org. Cult. 18: 231-280.
76. van Engelen FA, Schouten A, Molthoff JW, Roosien J, Salinas J, Dirkse WG, Schots A, Bakker J, Gommers FJ, Jongsma MA et al. (1994) Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco. Plant Mol. Biol. 26: 1701-1710.
77. Vaquero C, Sack M, Chandler J, Drossard J, Schuster F, Schillberg S & Fischer R (1999) Transient expression of a tumor-specific single chain fragment and a chimeric antibody in tobacco leaves. Proc. Natl. Acad. Sci. 96: 11128-11133.
78. Verwoerd TC, van Paridon PA, van Ooyen AJJ, van Lent JWM, Hoekema A & Pen J (1995) Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves. Plant Physiol. 109: 1199-1205.
79. Voss A, Niersbach M, Hain R, Hirsch H, Liao Y, Kreuzaler F & Fischer R (1995) Reduced virus infectivity in N. tabacum secreting a TMV-specific full size antibody. Mol. Breed. 1: 39-50.
80. Wen WS (1995) Bioprocessing technology for plant cell suspension cultures. Appl. Biochem. Biotechnol. 50: 189-216.
81. Whitelam G & Garry W (1996) Antibody expression in transgenic plants. Trends Plant Sci. 1: 268-271.
82. Willmitzer L, Depicker A, Dhaese P, De Greve H, Hernalsteens JP, Holsters M, Leemans J, Otten L, Schroder J, Schroder G, Zambryski P, van Montagu M & Schell J (1983) The use of Ti-plasmids as plant-directed gene vectors. Folia Biol. 29: 106-114.
83. Winter G, Griffiths AD, Hawkins RE & Hoogenboom HR (1994) Making antibodies by phage display technology. Ann. Rev. Immunol. 12: 433-455.
84. Winter G & Milstein C (1991) Man-made antibodies. Nature 349: 293-299.
85. Zeitlin L, Olmsted SS, Moench TR, Co MS, Martinell BJ, Paradkar VM, Russell DR, Queen C, Cone RA & Whaley KJ (1998) A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes. Nature Biotechnol. 16: 1361-1364.
86. Ziegler M, Thomas S & Danna K (2000) Accumulation of a thermostable endo-1,4-β-D-glucanase in the apoplast of Arabidopsis thaliana leaves. Mol. Breed. 6: 37-46.
87. Zimmermann S, Schillberg S, Liao YC & Fischer R (1998) Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum. Mol. Breed. 4: 369-379.