Plastid biotechnology: prospects for herbicide and insect resistance, metabolic engineering and molecular farming

Current Opinion in Biotechnology

Volumn 18, Issue 2, 2007, Pages 100-106

  Bock, Ralph ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DRUG PRODUCTS; GENE EXPRESSION; PLANTS (BOTANY); PROTEINS;

CHLOROPLASTS; NUCLEAR TRANSGENIC TECHNOLOGIES; PLASTID TRANSFORMATION;

GENETIC ENGINEERING;

HERBICIDE;

BIOTECHNOLOGY; CHLOROPLAST; GENETIC ENGINEERING; INSECT RESISTANCE; METABOLIC ENGINEERING; MOLECULAR FARMING; NONHUMAN; PLANT BIOTECHNOLOGY; PLASTID; PLASTID BIOTECHNOLOGY; PRIORITY JOURNAL; REVIEW;

AGRICULTURE; ANIMALS; BIOTECHNOLOGY; FORECASTING; GENETIC ENGINEERING; HERBICIDES; INSECTS; MOLECULAR BIOLOGY; PLANT DISEASES; PLANTS, GENETICALLY MODIFIED; PLASTIDS;

HEXAPODA;

EID: 34147158443     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.copbio.2006.12.001     Document Type: Review

References (40)

1. Boynton J.E., Gillham N.W., Harris E.H., Hosler J.P., Johnson A.M., Jones A.R., Randolph-Anderson B.L., Robertson D., Klein T.M., Shark K.B., and Sanford J.C. Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240 (1988) 1534-1538
2. Svab Z., Hajdukiewicz P., and Maliga P. Stable transformation of plastids in higher plants. Proc Natl Acad Sci USA 87 (1990) 8526-8530
3. McBride K.E., Svab Z., Schaaf D.J., Hogan P.S., Stalker D.M., and Maliga P. Amplification of a chimeric Bacillus gene in chloroplasts leads to an extraordinary level of an insecticidal protein in tobacco. Bio/Technology 13 (1995) 362-365
4. Bock R. Transgenic chloroplasts in basic research and plant biotechnology. J Mol Biol 312 (2001) 425-438
5. Maliga P. Plastid transformation in higher plants. Annu Rev Plant Biol 55 (2004) 289-313
6. Kota M., Daniell H., Varma S., Garczynski S.F., Gould F., and Moar W.J. Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects. Proc Natl Acad Sci USA 96 (1999) 1840-1845
7. De Cosa B., Moar W., Lee S.-B., Miller M., and Daniell H. Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol 19 (2001) 71-74
8. Chakrabarti S.K., Lutz K.A., Lertwiriyawong B., Svab Z., and Maliga P. Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers resistance to potato tuber moth. Transgenic Res 15 (2006) 481-488. This paper describes the deleterious effects of high-level Bt toxin expression from the plastid genome and highlights the importance of carefully determining the optimum expression level of resistance proteins.
9. Dufourmantel N., Tissot G., Goutorbe F., Garcon F., Muhr C., Jansens S., Pelissier B., Peltier G., and Dubald M. Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin. Plant Mol Biol 58 (2005) 659-668. The first reported success of engineering insect resistance into the plastid genome of an important food crop.
10. Daniell H., Datta R., Varma S., Gray S., and Lee S.-B. Containment of herbicide resistance through genetic engineering of the chloroplast genome. Nat Biotechnol 16 (1998) 345-348
11. Ye G.-N., Hajdukiewicz P.T.J., Broyles D., Rodriguez D., Xu C.W., Nehra N., and Staub J.M. Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco. Plant J 25 (2001) 261-270
12. Iamtham S., and Day A. Removal of antibiotic resistance genes from transgenic tobacco plastids. Nat Biotechnol 18 (2000) 1172-1176
13. Lutz K.A., Knapp J.E., and Maliga P. Expression of bar in the plastid genome confers herbicide resistance. Plant Physiol 125 (2001) 1585-1591
14. Ye G.-N., Colburn S.M., Xu C.W., Hajdukiewicz P.T.J., and Staub J.M. Persistence of unselected transgenic DNA during a plastid transformation and segregation approach to herbicide resistance. Plant Physiol 133 (2003) 402-410
15. Herz S., Füßl M., Steiger S., and Koop H.-U. Development of novel types of plastid transformation vectors and evaluation of factors controlling expression. Transgenic Res 14 (2005) 969-982. A conscientious and comprehensive analysis of expression signals that determine the level of foreign protein accumulation attainable in tobacco plastids. Different insertion sites, promoters and 5′ untranslated regions are compared and alternative strategies for designing plastid transformation vectors are outlined.
16. Birch-Machin I., Newell C.A., Hibberd J.M., and Gray J.C. Accumulation of rotavirus VP6 protein in chloroplasts of transplastomic tobacco is limited by protein stability. Plant Biotechnol J 2 (2004) 261-270
17. Andrews J.T., and Whitney S.M. Manipulating ribulose bisphosphate carboxylase/oxygenase in the chloroplasts of higher plants. Arch Biochem Biophys 414 (2003) 159-169
18. Bock R., and Khan M.S. Taming plastids for a green future. Trends Biotechnol 22 (2004) 311-318
19. Nakashita H., Arai Y., Shikanai T., Doi Y., and Yamaguchi I. Introducion of bacterial metabolism into higher plants by polycistronic transgene expression. Biosci Biotechnol Biochem 65 (2001) 1688-1691
20. Lössl A., Eibl C., Harloff H.-J., Jung C., and Koop H.-U. Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction. Plant Cell Rep 21 (2003) 891-899
21. Ruiz O.N., and Daniell H. Engineering cytoplasmic male sterility via the chloroplast genome by expression of β-ketothiolase. Plant Physiol 138 (2005) 1232-1246
22. Lössl A., Bohmert K., Harloff H., Eibl C., Mühlbauer S., and Koop H.-U. Inducible trans-activation of plastid transgenes: expression of the R. eutropha phb operon in transplastomic tobacco. Plant Cell Physiol 46 (2005) 1462-1471. A nice study demonstrating that the deleterious effects of constitutive expression of the bacterial polyhydroxybutyrate operon in chloroplasts can be circumvented by inducing plastid transgene expression with a nuclear-encoded and plastid-targeted ethanol-inducible T7 RNA polymerase.
23. Ruf S., Hermann M., Berger I.J., Carrer H., and Bock R. Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat Biotechnol 19 (2001) 870-875
24. Wurbs D, Ruf S, Bock R: Contained metabolic engineering in tomatoes by expression of carotenoid biosynthesis genes from the plastid genome. Plant J 2007, in press. The enhancement of provitamin A synthesis in transplastomic tomatoes as reported in this paper represents the first successful example of engineering a nutritionally important biochemical pathway in non-green plastids by transformation of the chloroplast genome. It is an encouraging step towards the application of plastid transformation technologies in food crops.
25. Raskin I., Ribnicky D.M., Komarnytsky S., Ilic N., Poulev A., Borisjuk N., Brinker A., Moreno D.A., Ripoll C., Yakoby N., et al. Plants and human health in the twenty-first century. Trends Biotechnol 20 (2002) 522-531
26. Ma J.K.-C., Barros E., Bock R., Christou P., Dale P.J., Dix P.J., Fischer R., Irwin J., Mahoney R., Pezzotti M., et al. Molecular farming for new drugs and vaccines. EMBO Rep 6 (2005) 593-599
27. Tregoning J.S., Nixon P., Kuroda H., Svab Z., Clare S., Bowe F., Fairweather N., Ytterberg J., van Wijk K.J., Dougan G., and Maliga P. Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucleic Acids Res 31 (2003) 1174-1179
28. Watson J., Koya V., Leppla S.H., and Daniell H. Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/feed crop. Vaccine 22 (2004) 4374-4384
29. Koya V., Moayeri M., Leppla S.H., and Daniell H. Plant-based vaccine: mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge. Infect Immun 73 (2005) 8266-8274. One of the first reports showing the efficacy of a chloroplast-produced vaccine protein. The protective antigen (PA) from B. anthracis was produced in tobacco chloroplasts and immunization with the purified PA protein protected mice from challenge with a lethal dose of anthrax toxin.
30. Glenz K., Bouchon B., Stehle T., Wallich R., Simon M.M., and Warzecha H. Production of a recombinant bacterial lipoprotein in higher plant chloroplasts. Nat Biotechnol 24 (2006) 76-77. An impressive paper describing the production of a Lyme disease vaccine in tobacco chloroplasts. The vaccine protein underwent N-terminal lipidation - a post-translational modification that is crucial for the immunogenicity of the protein and which was previously thought to be absent from chloroplasts.
31. 649-684, a mucosal subunit HIV/AIDS vaccine candidate. Vaccine 24 (2006) 5047-5055
32. Daniell H., Lee S.-B., Panchal T., and Wiebe P.O. Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J Mol Biol 311 (2001) 1001-1009
33. Limaye A., Koya V., Samsam M., and Daniell H. Receptor-mediated oral delivery of a bioencapsulated green fluorescent protein expressed in transgenic chloroplasts into the mouse circulatory system. FASEB J 20 (2006) E37-E46
34. Reed M.L., Wilson S.K., Sutton C.A., and Hanson M.R. High-level expression of a synthetic red-shifted GFP coding region incorporated into transgenic chloroplasts. Plant J 27 (2001) 257-265
35. Mason H.S., Warzecha H., Mor T., and Arntzen C.J. Edible plant vaccines: applications for prophylactic and therapeutic molecular medicine. Trends Mol Med 8 (2002) 324-329
36. Kumar S., Dhingra A., and Daniell H. Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56 (2004) 203-216
37. Dufourmantel N., Pelissier B., Garcon F., Peltier G., Ferullo J.-M., and Tissot G. Generation of fertile transplastomic soybean. Plant Mol Biol 55 (2004) 479-489
38. Magee A.M., Coyne S., Murphy D., Horvath E., Medgyesy P., and Kavanagh T.A. T7 RNA polymerase-directed expression of an antibody fragment transgene in plastids causes a semi-lethal pale-green seedling phenotype. Transgenic Res 13 (2004) 325-337
39. Padidam M. Chemically regulated gene expression in plants. Curr Opin Plant Biol 6 (2003) 163-177
40. Mühlbauer S.K., and Koop H.-U. External control of transgene expression in tobacco plastids using the bacterial lac repressor. Plant J 43 (2005) 941-946