Large-scale purification of an antibody directed against hepatitis B surface antigen from transgenic tobacco plants

Biochemical and Biophysical Research Communications

Volumn 308, Issue 1, 2003, Pages 94-100

  Valdés, Rodolfo ^a   Gómez, Leonardo ^a   Padilla, Sigifredo ^a   Brito, José ^a   Reyes, Biunayki ^a   ‹lvarez, Tatiana ^a   Mendoza, Otto ^a   Herrera, Orlando ^a   Ferro, William ^a   Pujol, Merardo ^a   Leal, Vladimir ^a   Linares, Marbelis ^a   Hevia, Yasser ^a   Garciÿ, Cristina ^a   Milÿ, Lorely ^a   Garciÿ, Olga ^a   Sÿnchez, Rafael ^a   Acosta, Alfonso ^a   Geada, Déborah ^a   Paez, Rolando ^a   Vega, Jorge Luis ^a   Borroto, Carlos ^a   more..

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Expanded bed adsorption; Monoclonal antibody; Plantibody; Tobacco; Transgenic plant

Indexed keywords

ANTIBODY; HEPATITIS B ANTIGEN; NICOTIANA TABACUM EXTRACT; PLANT EXTRACT; UNCLASSIFIED DRUG;

ABSORPTION CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANTIBODY PRODUCTION; ARTICLE; BIOMASS; CONTROLLED STUDY; MEDICINAL PLANT; NICOTIANA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ANIMALIA; MURINAE; NICOTIANA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 0041347563     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)01335-4     Document Type: Article

References (35)

1. Parastoo E., Alireza K., Nickolay N. Polypeptides of hepatitis B surface antigen produced in transgenic potato. Gene. 190:1997;107-111.
2. Fontirrochi G.M., Dueñaz M., Fernández de Cossio M.E., Fuentes P., Pérez M., Mainet D., Ayala M., Gavilondo J., Duarte C. A mouse hybridoma cell line secreting IgG and IgM with specificity for the hepatitis B surface antigen. Biotechol. Apl. 10:1993;24-30.
3. Pentón E., Muzio V., González G.M. The hepatitis B virus (HBV) infection y its prevention by a recombinant DNA viral surface antigen (rec-HBsAg) vaccine. Biotecnol. Apl. 11:1994;1-11.
4. Hendriksen C.F.M. A call for a European prohibition of monoclonal antibody (Mab) production by the ascites procedure in laboratory animals. ATLA. 26:1998;523-540.
5. Köhler G., Milstein C. Continuous culture of fused cells secreting antibodies of predefined specificity. Nature. 256:1975;485-497.
6. Brodeur B.R., Tsang P., Larose Y. Parameters affecting ascites tumor formation in mice and monoclonal antibody production. J. Immunol. Methods. 71:1984;265-272.
7. Brodeur B.R., Tsang P.S. High yield monoclonal antibody production in ascites. J. Immunol. Methods. 86:1986;239-241.
8. Stewart F., Callander A., Garwes D.J. Comparison of ascites production for monoclonal antibodies in BALB/c and BALB/c-derived cross-bred mice. J. Immunol. Methods. 119:1989;269-275.
9. Chandler J.P. Factors influencing monoclonal antibody production in mouse ascites fluid. Commercial Production of Monoclonal Antibodies: A Guide for Scale-up: SS. Seaver. 1987;75-92 Marcel Dekker, New York.
10. Birch J.R., Lambert K., Thompson P.W., Kenny A.C., Wood L.A. Antibody production with airlift fermentors. Lyderson B.K. Large-Scale Cell Culture Technology. 1987;1-20 Hanser Publications, New York.
11. Falkenberg F.W., Hengelage T., Krane M., Bartelsm I., Albrechtm A., Holtmeierm N. A simple and inexpensive high density dialysis tubing cell culture system for the in vitro production of monoclonal antibodies in high concentration. J. Immunol. Methods. 165:1993;193-206.
12. Evans T.L., Miller R.A. Large-scale production of murine monoclonal antibodies using hollow fiber bioreactors. Biotechniques. 6:1988;762-767.
13. Miele L. Plants as bioreactors for biopharmaceuticals: regulatory considerations. Tibetech February. 15:1997;45-50.
14. Hiatt A., Cafferkey R., Bowish K. Production of antibodies in transgenic plants. Nature. 342:1989;76-78.
15. Hansson M., Stahl S., Hjorth R., Uhlen M., Moks T. Single-step recovery of a secreted recombinant protein by expanded bed adsorption. Bio/technology. 12:1994;285-288.
16. Zurek H., Kubis E., Keup P., Hoerlein D., Beunink H., Tommes J., Kula M., Hollenberg C., Gellissen G. Production of two aprotinin variants in Hansenula polymorpha. Process Biochem. 31:1996;679-689.
17. Chang Y., Chase H. Ion exchange purification of G6PDH from unclarified yeast cell homogenates using expanded bed adsorption. Biotechnol. Bioeng. 49:1996;204-216.
18. Thömmes J., Halfar M., Lenz S., Kula M. Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption. Biotechnol. Bioeng. 45:1995;203-211.
19. Valdés R., Leyva J.L., González E., Mainet D., Costas L. Caracterización de anticuerpos monoclonales contra el antígeno de superficie del virus de la Hepatitis B. Biotecnol. Apl. 11(3):1994;219-224.
20. Lowry O.H., Rosenbroug N.J., Farr A.L., Randal R. Protein measurement with the Folinn phenol reagent. J. Biol. Chem. 193:1951;265-275.
21. Laemmli U.K. Cleavage of structural proteins during the assembly of head bacteriophage T4. Nature. 227:1970;680-685.
22. Little M., Kipriyanov M.S., Gall F.Le., Moldehauer G. Of mice and men: hybridomas and recombinant antibodies. Immunol. Today. 21(8):2000;364-369.
23. Wildman S.G. Tobacco a potential food crop. Crops Soils Mag. (January):1979;7-9.
24. M.N. Draeger, H.A. Chase, Liquid fluidized beds for protein purification, I. Chem. Eng. Symp. Ser. No. 118 (1990) 12.1-12.12.
25. Galliot F.P., Gleason C., Wilson J.J., Zwarick J. Fluidized bed adsorption for whole broth extraction. Biotech. Progr. 6:1990;370-375.
26. Barthels C.R., Kleinman G., Korzon N.J., Irish D.B. A novel ion-exchange method for the isolation of streptomycin. Chem. Eng. Prog. 54:1958;49-52.
27. Belter P.A., Cunningha F.L., Chen J.W. Development of a recovery process for novobocin. Biotechnol. Bioeng. 6:1973;533-549.
28. F. Barnfield, A. Johansson, R. Hjorth, Recovery of a recombinant protein from an E. coli homogenate using expanded bed adsorption, Poster Presented at Recovery of Biological Products VI, Engineering Foundation, Interlaken, Switzerland, September 1992.
29. A. Suding, M. Tomusiak, Protein recovery from E. coli homogenate using expanded bed adsorption chromatography, Presented at 205th American Chemical Society National Meeting, Denver, Colorado, USA, April 1993, Paper no. 61.
30. Chang Y.K., MacCreath G.E., Chase H.A. Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast cell homogenates. Biotech. Bioeng. 48:1995;355-366.
31. Noppe W., Hanssens I., Cuyper M. Simple two-step procedure for the preparation of highly active pure equine milk lysozyme. J. Chromatogr. 719:1996;327-331.i.
32. Garg N.Y., Yu I., Mattiason G., Mattiasson B. Polymer-shielded dye ligand chromatography of lactate deshydrogenase from porcine muscle in an expanded bed system. Bioseparations. 6:1996;193-199.
33. Fahrner R., Blank G., Zapata G. Expanded bed protein A affinity chromatography of a recombinant humanized monoclonal antibody: process development, operation and comparison with a packed bed method. J. Biotechnol. 75:1999;273-280.
34. Fischer R., Liao Y.C., Drossad J. Affinity purification of a TMV specific recombinant full-size antibody from a transgenic tobacco suspension culture. J. Immunol. Method. 226:1999;1-10.
35. Fernández de Cossio M.E., Valdés R., Agraz A., Pérez L., González M., García G., Valdivia I., Gavilondo J.V. Development of a mouse monoclonal antibody for the purification of a yeast-derived recombinant hepatitis B surface antigen. Minerva Biotechnol. 9:1997;76-84.