Molecular farming in plants: Host systems and expression technology

Trends in Biotechnology

Volumn 21, Issue 12, 2003, Pages 570-578

  Twyman, Richard M ^a   Stoger, Eva ^b   Schillberg, Stefan ^c   Christou, Paul ^c   Fischer, Rainer ^b,c  

^a UNIVERSITY OF YORK   (United Kingdom)

^b RWTH AACHEN UNIVERSITY   (Germany)

^c FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOREACTORS; DRUG PRODUCTS; PLANTS (BOTANY); PROTEINS;

PLANT-BASED EXPRESSION SYSTEMS;

GENETIC ENGINEERING;

OIL; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

BIOTECHNOLOGY;

BIOSAFETY; CEREAL; CROP; DRUG RESEARCH; FIBER; FRUIT; GENE EXPRESSION; GENE EXPRESSION SYSTEM; HUMAN; LEAFY VEGETABLE; LEGUME; NONHUMAN; PLANT BIOTECHNOLOGY; PLANT PRODUCT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN TARGETING; QUALITY CONTROL; REVIEW; SPECIES; TRANSGENE; TRANSGENIC PLANT;

EID: 0242475327     PISSN: 01677799     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tibtech.2003.10.002     Document Type: Review

References (86)

1. Hood E.E., et al. Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Mol. Breed. 3:1997;291-306.
2. Witcher D., et al. Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants. Mol. Breed. 4:1998;301-312.
3. Kusnadi A.R., et al. Processing of transgenic corn seed and its effect on the recovery of recombinant β-glucuronidase. Biotechnol. Bioeng. 60:1998;44-52.
4. Evangelista R.L., et al. Process and economic evaluation of the extraction and purification of recombinant β-glucuronidase from transgenic corn. Biotechnol. Prog. 14:1998;607-614.
5. Fischer R., Emans N. Molecular farming of pharmaceutical proteins. Transgenic Res. 9:2000;279-299.
6. Giddings G. Transgenic plants as protein factories. Curr. Opin. Biotechnol. 12:2001;450-454.
7. Hood, E.E. Plants as enzyme factories. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press).
8. Hood E.E., et al. Monoclonal antibody manufacturing in transgenic plants - myths and realities. Curr. Opin. Biotechnol. 13:2002;630-635.
9. Schillberg S., et al. Antibody molecular farming in plants and plant cells. Phytochem. Rev. 1:2002;45-54.
10. Mason H.S., et al. Edible plant vaccines: applications for prophylactic and therapeutic molecular medicine. Trends Mol. Med. 8:2002;324-329.
11. Denbow D.M., et al. Soybeans transformed with fungal phytase gene improve phosphorus availability for broilers. Poult. Sci. 77:1998;878-881.
12. Dai Z.Y., et al. Improved plant-based production of E1 endoglucanase using potato: expression optimization and tissue targeting. Mol. Breed. 6:2000;277-285.
13. Ziegler M.T., et al. Accumulation of a thermostable endo-1,4-β-D- glucanase in the apoplast of Arabidopsis thaliana leaves. Mol. Breed. 6:2000;37-46.
14. Ziegelhoffer T., et al. Dramatic effects of truncation and sub-cellular targeting on the accumulation of recombinant microbial cellulase in tobacco. Mol. Breed. 8:2001;147-158.
15. Perrin Y., et al. Transgenic pea seeds as bioreactors for the production of a single-chain Fv fragment (scFV) antibody used in cancer diagnosis and therapy. Mol. Breed. 6:2000;345-352.
16. Stoger E., et al. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol. Biol. 42:2000;583-590.
17. Moloney, M., et al. (2003) Oil bodies and associated proteins as affinity matrices. US Patent 6509453.
18. Schillberg S., et al. Plasma membrane display of anti-viral single chain Fv fragments confers resistance to tobacco mosaic virus. Mol. Breed. 6:2000;317-326.
19. Borisjuk N.V., et al. Production of recombinant proteins in plant root exudates. Nat. Biotechnol. 17:1999;466-469.
20. Komarnytsky S., et al. Production of recombinant proteins in tobacco guttation fluid. Plant Physiol. 124:2000;927-933.
21. Drake P.M.W., et al. Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots. Plant Mol. Biol. 52:2003;233-241.
22. Fischer R., et al. Towards molecular farming in the future: transient protein expression in plants. Biotechnol. Appl. Biochem. 30:1999;113-116.
23. Fischer R., et al. Towards molecular farming in the future: using plant-cell-suspension cultures as bioreactors. Biotechnol. Appl. Biochem. 30:1999;109-112.
24. Commandeur U., et al. The biosafety of molecular farming in plants. AgBiotechNet. 5:2003;110.
25. Stoger E., et al. Plantibodies: applications, advantages and bottlenecks. Curr. Opin. Biotechnol. 13:2002;161-166.
26. Ma J.K., et al. Generation and assembly of secretory antibodies in plants. Science. 268:1995;716-719.
27. Larrick J.W., et al. Production of secretory IgA antibodies in plants. Biomol. Eng. 18:2001;87-94.
28. Warner T.G. Metabolic engineering glycosylation: biotechnology's challenge to the glycobiologist in the new millennium. Ernst B., et al. Carbohydrates in Chemistry and Biology. 2000;1043-1064 Wiley-VCH.
29. Blixt O., et al. Efficient chemoenzymatic synthesis of O-linked sialyl oligosaccharides. J. Am. Chem. Soc. 124:2002;5739-5746.
30. Bakker H., et al. Galactose-extended glycans of antibodies produced by transgenic plants. Proc. Natl. Acad. Sci. U. S. A. 98:2001;2899-2904.
31. Chargelegue D., et al. A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice. Transgenic Res. 9:2000;187-194.
32. Tyagi A.K. Plant genes and their expression. Curr. Sci. 80:2001;161-169.
33. Christensen A.H., Quail P.H. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res. 5:1996;213-218.
34. Vain P., et al. Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.). Plant Cell Rep. 15:1996;489-494.
35. Zuo J., Chua N.-H. Chemical-inducible systems for regulated expression of plant genes. Curr. Opin. Biotechnol. 11:2000;146-151.
36. Cramer C.L., et al. Transgenic plants for therapeutic proteins: linking upstream and downstream technologies. Curr. Top. Microbiol. Immunol. 240:1999;95-118.
37. Gutiérrez R.A., et al. Current perspectives on mRNA stability in plants: multiple levels and mechanisms of control. Trends Plant Sci. 4:1999;429-438.
38. Kawaguchi R., Bailey-Serres J. Regulation of translational initiation in plants. Curr. Opin. Plant Biol. 5:2002;460-465.
39. Koziel M.G., et al. Optimizing expression of transgenes with an emphasis on post-transcriptional events. Plant Mol. Biol. 32:1996;393-405.
40. Plasterk R.H.A., Ketting R.F. The silence of the genes. Curr. Opin. Genet. Dev. 10:2000;562-567.
41. Twyman R.M., et al. Foreign DNA: integration and expression in transgenic plants. Setlow J.K. Genetic Engineering: Principles and Practice. Vol. 24:2002;107-136 Kluwer-Plenum Press.
42. Voinnet O. RNA silencing: small RNAs as ubiquitous regulators of gene expression. Curr. Opin. Plant Biol. 5:2002;444-451.
43. Kohli A., et al. Transgene integration, organization and interaction in plants. Plant Mol. Biol. 52:2003;247-258.
44. Fu X.D., et al. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Res. 9:2000;11-19.
45. Zimmermann S., et al. Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum. Mol. Breed. 4:1998;369-379.
46. Schillberg S., et al. Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum. Transgenic Res. 8:1999;255-263.
47. De Jaeger G., et al. High-level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida. Eur. J. Biochem. 259:1999;426-434.
48. Schouten A., et al. Formation of disulfide bridges by a single-chain Fv antibody in the reducing ectopic environment of the plant cytosol. J. Biol. Chem. 277:2002;19339-19345.
49. Nuttall J., et al. ER-resident chaperone interactions with recombinant antibodies in transgenic plants. Eur. J. Biochem. 269:2002;6042-6051.
50. Conrad U., Fiedler U. Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Mol. Biol. 38:1998;101-109.
51. Sriraman, R., et al. Glycosylation of recombinant antibodies in plants. Proceedings of the Ranbaxy Science Foundation Symposium 2002, Ranbaxy Science Foundation, New Delhi (in press).
52. Daniell H., et al. Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci. 7:2002;84-91.
53. Staub J.M., et al. High-yield production of a human therapeutic protein in tobacco chloroplasts. Nat. Biotechnol. 18:2000;333-338.
54. Millan A.F.-S., et al. A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein highly susceptible to proteolytic degradation. Plant Biotechnol. 1:2003;77-79.
55. Daniell H., et al. Expression of the native cholera B toxin subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J. Mol. Biol. 311:2001;1001-1009.
56. Tregoning J.S., et al. Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucleic Acids Res. 31:2003;1174-1179.
57. Leelavathi S., et al. Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme. Mol. Breed. 11:2003;59-67.
58. Kay E., et al. In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria. Appl. Environ. Microbiol. 68:2002;3345-3351.
59. Jobling S.A., et al. Immunomodulation of enzyme function in plants by single domain antibody fragments. Nat. Biotechnol. 21:2003;77-80.
60. Stöger E., et al. Practical considerations for pharmaceutical antibody production in different crop systems. Mol. Breed. 9:2002;149-158.
61. D'Aoust, M.A. Perennial plants as a production system for pharmaceuticals. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press).
62. Zeitlin L., et al. A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes. Nat. Biotechnol. 16:1998;1361-1364.
63. Khoudi H., et al. Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol. Bioeng. 64:1999;135-143.
64. Kapusta J., et al. A plant-derived edible vaccine against hepatitis B virus. FASEB J. 13:1999;1796-1799.
65. Hood E.E. From green plants to industrial enzymes. Enzyme & Microbial Technol. 30:2002;279-283.
66. Vierling R.A., Wilcox J.R. Microplate assay for soybean seed coat peroxidase activity. Seed Science Technol. 24:1996;485-494.
67. Tacket C.O., et al. Immunogenicity in humans of a recombinant bacterial-antigen delivered in transgenic potato. Nat. Med. 4:1998;607-609.
68. Tacket C.O., et al. Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes. J. Infect. Dis. 182:2000;302-305.
69. Richter L.J., et al. Production of hepatitis B surface antigen in transgenic plants for oral immunization. Nat. Biotechnol. 18:2000;1167-1171.
70. Yu J., Langridge W. Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice. Transgenic Res. 12:2003;163-169.
71. Artsaenko O., et al. Potato tubers as a biofactory for recombinant antibodies. Mol. Breed. 4:1998;313-319.
72. De Wilde C., et al. Expression of antibodies and Fab fragments in transgenic potato plants: a case study for bulk production in crop plants. Mol. Breed. 9:2002;2871-2882.
73. Schunmann P.H.D., et al. Biopharming the SimpliRED™ HIV diagnostic reagent in barley, potato and tobacco. Mol. Breed. 9:2002;113-121.
74. Chong D.K.X., Langridge W.H.R. Expression of full-length bioactive antimicrobial human lactoferrin in potato plants. Transgenic Res. 9:2000;71-78.
75. Chong D.K.X., et al. Expression of the human milk protein β-casein in transgenic potato plants. Transgenic Res. 6:1997;289-296.
76. McGarvey P.B., et al. Expression of the rabies virus glycoprotein in transgenic tomatoes. Biotechnology (N.Y.). 13:1995;1484-1487.
77. Sala F., et al. Vaccine antigen production in transgenic plants: strategies, gene constructs and perspectives. Vaccine. 21:2003;803-808.
78. Kapila J., et al. An agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci. 122:1997;101-108.
79. Vaquero C., et al. A carcinoembryonic antigen-specific diabody produced in tobacco. FASEB J. 16:2002;408-410.
80. Verch T., et al. Expression and assembly of a full-length monoclonal antibody in plants using a plant virus vector. J. Immunol. Methods. 220:1998;69-75.
81. McCormick A.A., et al. Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proc. Natl. Acad. Sci. U. S. A. 96:1999;703-708.
82. Mor, T.S. and Mason, H.S. Plants as a source of subunit vaccines. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press).
83. Doran P.M. Foreign protein production in plant tissue cultures. Curr. Opin. Biotechnol. 11:2000;199-204.
84. Torres E., et al. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies. Transgenic Res. 8:1999;441-449.
85. Bardor M., et al. Immunoreactivity in mammals of two typical plant glyco-epitopes, core α(1,3)-fucose and core xylose. Glycobiology. 13:2003;427-434.
86. Lerouge P., et al. N-glycoprotein biosynthesis in plants: recent developments and future trends. Plant Mol. Biol. 38:1998;31-48.