Molecular farming of pharmaceutical proteins

Transgenic Research

Volumn 9, Issue 4-5, 2000, Pages 279-299

  Fischer, Rainer ^a   Emans, Neil ^b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b IUCT   (Germany)

Author keywords

Antibody; Biotechnology; Expression; Molecular farming; Purification; Recombinant protein; Transgenic plant

Indexed keywords

BIOMASS PRODUCTION; GENETIC TRANSFORMATION; INDUSTRY; MOLECULAR FARMING; PLANT BREEDING; RECOMBINANT PROTEIN; SUSPENSION CELL CULTURE; TRANSGENIC PLANT;

EID: 0033667765     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008975123362     Document Type: Review

References (161)

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4. Potato tubers as a biofactory for recombinant antibodies
5. Expression of a single-chain Fv antibody against abscisic acid creates a wilty phenotype in transgenic tobacco
6. Regulatory agency concerns in the manufacturing and testing of monoclonal antibodies for therapeutic use
7. Analysis of the N-glycosylation of recombinant glycoproteins produced in transgenic plants
8. The expression of a nopaline synthase-human growth hormone chimaeric gene in transformed tobacco and sunflower callus tissue
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12. Biological therapy of ovarian cancer: Current directions
13. Production of recombinant proteins in plant root exudates
14. A trout growth hormone is expressed, correctly folded and partially glycosylated in the leaves but not the seed of transgenic plants
15. Bacterial and plant-produced scFv proteins have similar antigen-binding properties
16. N-Glycosylation of a mouse IgG expressed in transgenic tobacco plants
17. Determination of the pore size of cell walls of living plant cells
18. Protective immune response to foot-and-mouth disease virus with VP1 expressed in transgenic plants
19. Agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/beta-glucuronidase gene
20. Expression of alpha-amylases, carbohydrate metabolism, and autophagy in cultured rice cells is coordinately regulated by sugar nutrient
21. Production of secretory immunoglobulin A by a single mammalian cell
22. Particle gun-mediated transformation
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28. Changes in protein methylation associated with the elicitation response in cell cultures of alfalfa (Medicago sativa L.)
29. High level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida
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31. Intact antigen-binding MAK33 antibody and Fab fragment accumulate in intercellular spaces of Arabidopsis thaliana
32. The expression, localization, and effect of a human interferon in plants
33. Elicitor-induced generation of active oxygen in suspension cultures of Arabidopsis thaliana
34. Human haemoglobin from transgenic tobacco
35. Synthesis and selfassembly of a functional monoclonal antibody in transgenic Nicotiana tabacum
36. Recombinant protein production in transgenic animals
37. Polypeptides of hepatitis B surface antigen produced in transgenic potato
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40. Nicotiana benthamiana plants expressing beet necrotic yellow vein virus (BNYVV) coat protein-specific scFv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection
41. High-level production and long-term storage of engineered antibodies in transgenic tobacco seeds
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44. Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures
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46. Toward molecular farming in the future: Using plant cell suspension cultures as bioreactors
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48. Expression and characterization of bispecific single chain Fv fragments produced in transgenic plants
49. Expression and characterization of bryodin 1 and a bryodin 1-based single-chain immunotoxin from tobacco cell culture
50. Functional expression in bacteria and plants of an scFv antibody fragment against tospoviruses
51. Recombinant proteins from transgenic plants
52. Controlling gene expression in transgenics
53. Costimulation of T-cell proliferation by a chimeric B7-antibody fusion protein
54. Co-introduction of an antisense gene for an endogenous seed storage protein can increase expression of a transgene in Arabidopsis thaliana seeds
55. Oral immunization with a recombinant bacterial antigen produced in transgenic plants
56. Rapid production of single-chain Fv fragments in plants using a potato virus X episomal vector
57. Antibodies produced in plants
58. Production of antibodies in transgenic plants
59. Characterization and applications of antibodies produced in plants
60. Assembly of antibodies and mutagenized variants in transgenic plants and plant cell cultures
61. Production of monoclonal antibody in plants
62. Transformation of rice mediated by Agrobacterium tumefaciens
63. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA
64. Expression of a chemically synthesized gene for human epidermal growth factor under the control of cauliflower mosaic virus 35S promoter in transgenic tobacco
65. Growth and nicotinate biotransformation in batch cultured and airlift fermenter grown soybean cell suspension cultures
66. Production of a foreign protein product with genetically modified plant cells
67. Commercial production of Avidin from transgenic maize: Characterization of transformant, production, processing, extraction and purification
68. Molecular farming of industrial proteins from transgenic maize
69. Cultivation of plant cells in a stirred tank reactor
70. A simple and general method for transferring genes into plants
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74. Production of a diagnostic monoclonal antibody in perennial alfalfa plants
75. Plant cell suspension cultures: Some engineering considerations
76. Continuous cultures of fused cells secreting antibody of predefined specificity
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78. Rapid, high-level expression of glycosylated rice alpha-amylase in transfected plants by an RNA viral vector
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83. Establishment of a transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony stimulating factor
84. Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants
85. Transient gene expression in electroporated protoplasts and intact cells of sugar beet
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88. Plant antibodies for immunotherapy
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91. Plant expression systems for the production of vaccines
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94. Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture
95. Validating the preparation of clinical monoclonal antibodies
96. Transgenic plants as vaccine production systems
97. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice
98. Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): Potatoes expressing a synthetic LT-B gene
99. Expression of hepatitis B surface antigen in transgenic plants
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101. Isolation of erythropoietin receptor agonist peptides using evolved phage libraries
102. Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants
103. Expression of the rabies virus glycoprotein in transgenic tomatoes
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105. Immunization against rabies with plant-derived antigen
106. Subcellular targeting and purification of recombinant proteins in plant production systems
107. Expression of human muscarinic cholinergic receptors in tobacco
108. FDA perspective on specifications for biotechnology products - From IND to PLA
109. Purification of monoclonal antibodies by epitope and mimotope affinity chromatography
110. Tobacco BY-2 cell line as the 'HeLa' cell in the cell biology of higher plants
111. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins
112. Synthesis of a functional anti-phytochrome single-chain Fv protein in transgenic tobacco
113. Production of biologically active hirudin in plant seeds using oleosin partitioning
114. Seed-specific immunomodulation of abscisic acid activity induces a developmental switch
115. Transgenic plants expressing human glutamic acid decarboxylase (GAD65), a mojor autoantigen in insulin-dependent diabetes mellitus
116. Use of viral replicons for the expression of genes in plants
117. The development of cowpea mosaic virus as a potential source of novel vaccines
118. Plasma membrane display of anti-viral single chain Fv fragments confers resistance to tobacco mosaic virus
119. Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum
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121. Improving scFv antibody expression levels in the plant cytosol
122. The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both cytosol and the secretory pathway in transgenic tobacco
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124. Biomass and chemical composition of tobacco plants under high density growth
125. Vectors based on maize streak virus can replicate to high copy numbers in maize plants
126. Hybrid antibodies
127. Production of correctly processed human serum albumin in transgenic plants
128. Bacterial expression of immunoglobulin fragments
129. High-yield production of a human therapeutic protein in tobacco chloroplasts
130. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies
131. Development of biopharmaceuticals in plant expression systems: Cloning, expression and immunological reactivity of human cytomegalovirus glycoprotein B (UL55) in seeds of transgenic tobacco
132. Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato
133. Large scale insect and plant cell culture
134. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack
135. Production of functional human alpha 1-antitrypsin by plant cell culture
136. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies
137. Malarial epitopes expressed on the surface of recombinant tobacco mosaic virus
138. Cell and tissue cultures of Catharanthus-Roseus LG Don a literature survey
139. Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco
140. Transient expression of a tumor-specific single chain fragment and a chimeric antibody in tobacco leaves
141. Expression and assembly of a full-length monoclonal antibody in plants using a plant virus vector
142. Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves
143. Reduced virus infectivity in N. tabacum secreting a TMV-specific full size antibody
144. Plants for delivery of edible vaccines
145. Pharmaceuticals, biologics and biopharmaceuticals
146. Heterologous protein production in transgenic plants
147. Antibody production in transgenic plants
148. Antibody expression in transgenic plants
149. Induction of a protective antibody response to foot and mouth disease virus in mice following oral or parenteral immunization with alfalfa transgenic plants expressing the viral structural protein VP1
150. Making antibodies by phage display technology
151. Man-made antibodies
152. Commercial production of b-glucuronidase (GUS): A model system for the production of proteins in plants
153. Correlation between in vitro stability and in vivo performance of anti- GCN4 intrabodies as cytoplasmic inhibitors
154. Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1
155. A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes
156. Commercial production of Aprotinin in transgenic maize seeds
157. Expression of human alpha-interferon in plants
158. Accumulation of a thermostable endo-1,4-b-D-glucanase in the apoplast of Arabidopsis thaliana leaves
159. Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum
160. Phage-displayed peptide libraries