Production of recombinant proteins in plant root exudates

Nature Biotechnology

Volumn 17, Issue 5, 1999, Pages 466-469

  Borisjuk, Niolai V ^a   Borisjuk, Ludmyla G ^a   Logendra, Sithes ^a   Petersen, Frank ^b   Gleba, Yuri ^c   Raskin, Ilya ^a  

^a RUTGERS UNIVERSITY   (United States)

^b RUTGERS UNIVERSITY   (United States)

^c NONE

Author keywords

Agricultural biotechnology; Hydroponic; Protein secretion; Recombinant proteins; Transgenic plants

Indexed keywords

ALKALINE PHOSPHATASE; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ACETYLATION; AGRICULTURE; AMINO ACID SEQUENCE; ARTICLE; BIOTECHNOLOGY; CLOSTRIDIUM THERMOCELLUM; ENDOPLASMIC RETICULUM; GLYCOSYLATION; JELLYFISH; NONHUMAN; PHOSPHORYLATION; PLANT ROOT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECRETION; TOBACCO; TRANSGENIC PLANT; WESTERN BLOTTING;

ALKALINE PHOSPHATASE; ANIMALS; CLONING, MOLECULAR; CLOSTRIDIUM BOTULINUM; GREEN FLUORESCENT PROTEINS; HUMANS; HYDROPONICS; LUMINESCENT PROTEINS; PLACENTA; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; RECOMBINANT PROTEINS; SCYPHOZOA; TOBACCO; XYLAN ENDO-1,3-BETA-XYLOSIDASE; XYLOSIDASES;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 0032916112     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/8643     Document Type: Article

References (25)

1. Conrad, U. & Fiedler, U. Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production of physiological functions and pathogen activity. Plant Mol. Biol. 38, 101-109 (1998).
2. Firek, S. et al. Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures. Plant Mol. Biol. 23, 861-670 (1993).
3. Wongsamuth, R. & Doran, P.M. Production of monoclonal antibodies by tobacco hairy roots. Biotechnology & Bioengineering. 54, 401-415 (1997).
4. 2. Annals of Botany 72, 155-163 (1993).
5. Marschner, H. Mineral Nutrition of Higher Plants (The University Press, Belfast, Northern Ireland, 1986).
6. Denecke, J., Botterman, J. & Deblaere, R. Protein secretion in plant cells can occur via a default pathway. Plant Cell 2, 51-59 (1990).
7. Reichel, C. et al. Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells. Proc. Natl. Acad. Sci. USA 93, 5888-5893 (1996).
8. Berger, J., Hauber, J., Hauber, R., Geiger, R. & Cullen, B.R. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66, 1-10 (1988).
9. Herbers, K., Wilke, I., and Sonnewald U. 1995. A thermostable xylanase from Clostridium thermocellum expressed at high levels in the apoplast of transgenic tobacco has no detrimental effects and is easily purified. Bio/Technology 13, 63-66.
10. Benfey, P.N., Ren, L. & Chua, N-H. The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J. 8, 2195-2202 (1989).
11. Ni, M., Cui, D. & Gelvin, S.B. Sequence-specific interactions of wound-inducible nuclear factors with mannopine synthase 2′ promoter wound responsive elements. Plant Mol. Biol. 30, 77-96 (1996).
12. Biely, P., Mislovicova, D. & Toman, R. Remazol Brilliant Blue xylan: a soluble chromogenic substrate for xylanases. Methods Enzymol. 160, 536-542 (1988).
13. Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G. & Cormier, M.J. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111, 229-233 (1992).
14. Borisjuk, N. et al. Calreticulin in plant cells: primary structure, developmentally regulated expression and intracellular distribution. Planta 206, 504-514 (1998).
15. Horsch, R.B. et al. A simple and general method for transferring genes into plants. Science 227, 1229-1231 (1985).
16. Davis, S.J. & Viestra, R.D. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol. Biol. 36, 521-528 (1998)
17. Hawes, M.C. & Brigham, L.A. Impact of root border cells on microbial populations in the rhizosphere. Adv. Plant Pathol. 8, 119-148 (1992).
18. Smith, R.A., Duncan, M.J. & Moir, D.T. Heterologous protein secretion from yeast. Science 229, 1219-1224 (1985).
19. Ursin, V.M. & Shewmaker C.K. Demonstration of a senescence component in the regulation of the mannopine synthase promoter. Plant Physiol. 102, 1033-1036 (1993).
20. Hood, E.E. et al. Commercial production of avidin from transgenic maize - characterization of transformant, production, processing, extraction and purification. Molecular Breeding 3, 291-306 (1997).
21. Reisman, H.R. Problems in scale-up of biotechnology product processing. Crit. Rev. Biotechnol. 13, 195-253 (1993).
22. Bevan, M. Binary Agrobacterium vectors for plant transformation, Nucl. Acids Res. 12, 8711-8721 (1984).
23. Chomczynski, P. & Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chtoroform extraction. Anal. Biochem. 162, 156-159 (1987).
24. Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).
25. Terry, M.E. & Bonner, B.A. An examination of centrifugation as a method of extracting an extracellular solution from peas, and its use for the study of indoleacetic acid-induced growth. Plant Physiol. 66, 321-325 (1980).