Plant-derived mouse IgG monoclonal antibody fused to KDEL endoplasmic reticulum-retention signal is N-glycosylated homogeneously throughout the plant with mostly high-mannose-type N-glycans

Plant Biotechnology Journal

Volumn 3, Issue 4, 2005, Pages 449-457

  Triguero, Ada ^a   Cabrera, Gleysin ^a   Cremata, José A ^a   Yuen, Chun Ting ^b   Wheeler, Jun ^b   Ramírez, Nadia I ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

Author keywords

ER retention signal; Glycosylation; KDEL; Oligomannosides; Plantibody; Tobacco plant

Indexed keywords

MAMMALIA; MURINAE; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 33646842291     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2005.00137.x     Document Type: Article

References (37)

1. Anders D.A. Dickerson I.M. Dixon J.E. ( 1990) Variants of the carboxyl-terminal KDEL sequence direct intracellular retention. J. Biol. Chem. 265, 5952 5955.
2. Bardor M. Faveeuw C. Fitchette A.C. Gilbert D. Galas L. Trottein F. Faye L. Lerouge P. ( 2003) Immunoreactivity in mammals of two typical plant glyco-epitopes, core α(1,3)-fucose and core xylose. Glycobiology, 13, 427 434.
3. Bigge J.C. Patel T.P. Bruce J.A. Goulding P.N. Charles S.M. Parekh R.B. ( 1995) Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. Anal. Biochem. 230, 229 238.
4. Bouquin T. Thomsen M. Nielsen L.K. Green T.H. Mundy J. Dziegie M.H. ( 2002) Human anti-rhesus D IgG1 antibody produced in transgenic plants. Transgenic Res. 11, 115 122.
5. Cabanes-Macheteau M. Fitchette-Laine A.C. Loutelier-Bourhis C. Lange C. Vine N.D. Ma J.K. Lerouge P. Faye L. ( 1999) N-glycosylation of a mouse IgG expressed in transgenic tobacco plants. Glycobiology, 9, 365 372.
6. Conrad U. Fiedler U. ( 1998) Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Mol. Biol. 38, 101 109.
7. During K. Hippe S. Kreuzaler F. Schell J. ( 1990) Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum. Plant Mol. Biol. 15, 281 293.
8. Elbers I.J.W. Stoopen G.M. Bakker H. Stevens L.H. Bardor M. Molthoff J.W. Jordi W.J. Bosch D. Lommen A. ( 2001) Influence of growth conditions and developmental stage on N-glycan heterogeneity of transgenic immunoglobulin G and endogenous proteins in tobacco leaves. Plant Physiol. 126, 1314 1322.
9. Faye L. Gomord V. Fitchette-Lainé A.C. Chrispeels M.J. ( 1993) Affinity purification of antibodies specific for Asn-linked glycans containing α 1,3 fucose or β 1,2 xylose. Anal. Biochem. 209, 104 108.
10. Frigerio L. Pastres A. Prada A. Vitale A. ( 2001) Influence of KDEL on the fate of trimeric or assembly-defective phaseolin: selective use of an alternative route to vacuoles. Plant Cell, 13, 1109 1126.
11. Galili G. Sengupta-Gopalan C. Ceriotti A. ( 1998) The endoplasmic reticulum of plant cells and its role in protein maturation and biogenesis of oil bodies. Plant Mol. Biol. 38, 1 29.
12. Gomord V. Denmat L.A. Fitchette-Laine A.C. Satiat-Jeunemaitre B. Hawes C. Faye L. ( 1997) The C-terminal HDEL sequence is sufficient for retention of secretory proteins in the endoplasmic reticulum (ER) but promotes vacuolar targeting of proteins that escape the ER. Plant J. 11, 313 325.
13. Gomord V. Faye L. ( 1996) Signals and mechanisms involved in intracellular transport of secreted proteins in plants. Plant Physiol. Biochem. 34, 165 182.
14. Helenius A. Aebi M. ( 2001) Intracellular functions of N-linked glycans. Science, 291, 2364 2369.
15. Herbers K. Sonnewald U. ( 1999) Production of new/modified proteins in transgenic plants. Curr. Opin. Biotechnol. 10, 163 168.
16. Hiatt A. Caffertey R. Bowdish K. ( 1989) Production of antibodies in transgenic plants. Nature, 342, 76 78.
17. Jefferis R. Lund J. Pound J.D. ( 1998) IgG-Fc-mediated effector functions: molecular definition of interaction sites for effector ligands and the role of glycosylation. Immunol. Rev. 163, 59 76.
18. Jenkins N. Parekh R.B. James D.C. ( 1996) Getting the glycosylation right: implications for the biotechnology industry. Nat. Biotechnol. 14, 975 981.
19. Khoudi H. Laberge S. Ferullo J.M. Bazin R. Darveau A. Castonguay Y. Allard G. Lemieux R. Vezina L.P. ( 1999) Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol. Bioeng. 64, 135 143.
20. Ko K. Tekoah Y. Rudd P.M. Harvey D.J. Dwek R.A. Spitsin S. Hanlon C.A. Rupprecht C. Dietzschold B. Golovkin M. Koprowski H. ( 2003) Function and glycosylation of plant-derived antiviral monoclonal antibody. Proc. Natl. Acad. Sci. USA, 100, 8013 8018.
21. Lerouge P. Cabanes-Macheteau M. Rayon C. Fischette-Laine A.C. Gomord V. Faye L. ( 1998) N-glycoprotein biosynthesis in plants: recent developments and future trends. Plant Mol. Biol. 38, 31 48.
22. Ma J.K. Hikmat B.Y. Wycoff K. Vine N.D. Chargelegue D. Yu L. Hein M.B. Lehner T. ( 1998) Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nat. Med. 4, 601 606.
23. Munro S. Pelham H.R. ( 1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell, 48, 899 907.
24. Navazio L. Miuzzo M. Royle L. Baldan B. Varotto S. Merry A.H. Harvey D.J. Dwek R.A. Rudd P.M. Mariani P. ( 2002) Monitoring endoplasmic reticulum-to-Golgi traffic of a plant calreticulin by protein glycosylation analysis. Biochemistry, 41, 14 141 14 149.
25. Pagny S. Cabanes-Macheteau M. Gillikin J.W. Leborgne-Castel N. Lerouge P. Boston R.S. Faye L. Gomord V. ( 2000) Protein recycling from the Golgi apparatus to the endoplasmic reticulum in plants and its minor contribution to calreticulin retention. Plant Cell, 12, 739 756.
26. Pelham H.R. ( 1988) Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment. EMBO J. 4, 913 918.
27. Ramirez N. Rodriguez M. Ayala M. Cremata J.A. Perez M. Martinez A. Linares M. Hevia Y. Paez R. Valdes R. Gavilondo J.V. Selman-Housein G. ( 2003) Expression and characterization of an anti-(hepatitis B surface antigen) glycosylated mouse antibody in transgenic tobacco (Nicotiana tabacum) plants and its use in the immunopurification of its target antigen. Biotechnol. Appl. Biochem. 38, 223 230.
28. van Ree R. Cabanes-Macheteau M. Akkerdaas J. Milazzo J.P. Loutelier-Bourhis C. Rayon C. Villalba M. Koppelman S. Aalberse R. Rodriguez R. Faye L. Lerouge P. ( 2000) Beta(1,2)-xylose and alpha(1,3)-fucose residues have a strong contribution in IgE binding to plant glycoallergens. J. Biol. Chem. 275, 11 451 11 458.
29. Rudd P.M. Elliott T. Cresswell P. Wilson I.A. Dwek R.A. ( 2001) Glycosylation and the immune system. Science, 291, 2370 2376.
30. Sharp J.M. Doran P.M. ( 2001) Characterization of monoclonal antibody fragments produced by plant cells. Biotechnol. Bioeng. 73, 338 346.
31. Sriraman R. Bardor M. Sack M. Vaquero C. Faye L. Fischer R. Finnern R. Lerouge P. ( 2004) Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core-α(1,3)-fucose residues. Plant Biotechnol. J. 2, 279 287.
32. Stevens L.H. Stoopen G.M. Elbers I.J. Molthoff J.W. Bakker H.A. Lommen A. Bosch D. Jordi W. ( 2000) Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco. Plant Physiol. 124, 173 182.
33. Tekoah Y. Ko K. Koprowski H. Harvey D.J. Wormald M.R. Dwek R.A. Rudd P.M. ( 2004) Controlled glycosylation of therapeutic antibodies in plants. Arch. Biochem. Biophys. 426, 266 278.
34. Valdés R. Ibarra N. Gómez M. Alvarez T. García J. Llambias R. Pérez C.A. Quintero O. Fischer R. ( 2001) CB.Hep1 hybridoma growth and antibody production using protein-free medium in a hollow fiber bioreactor. Cytechnol. 35, 145 154.
35. Valdés R. Reyes B. Alvarez T. García J. Montero J.A. Figueroa A. Gómez L. Padilla S. Geada D. Abrahantes M.C. Dorta L. Fernández D. Mendoza O. Ramírez N. Rodriguez M. Pujol M. Borroto C. Brito J. ( 2003) Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale. Biochem. Biophys. Res. Commun. 310, 742 747.
36. Wright A. Morrison S.L. ( 1997) Effect of glycosylation on antibody function: implications for genetic engineering. Trends Biotechnol. 15, 26 32.
37. Zeitlin L. Olmsted S.S. Moench T.C. Co M.S. Martinell B.J. Paradkar V.M. Russell D.R. Queen C. Cone R.A. Whaley K.J. ( 1998) A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes. Nat. Biotechnol. 16, 1361 1364.