Biopharming the SimpliRED™ HIV diagnostic reagent in barley, potato and tobacco

Molecular Breeding

Volumn 9, Issue 2, 2002, Pages 113-121

  Schünmann, Petra H D ^a   Coia, Greg ^a   Waterhouse, Peter M ^a  

^a CSIRO   (Australia)

Author keywords

Antibody; Bioreactor; Diagnostic; Fusion protein; Molecular pharming; Recombinant

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; BIOCONVERSION; CHEMICAL MODIFICATION; DIAGNOSIS; DISEASES; EPITOPES; GENE EXPRESSION; GRAIN (AGRICULTURAL PRODUCT); IMMUNE SYSTEM; MONOCLONAL ANTIBODIES; PLANTS (BOTANY); RECOMBINANT PROTEINS; SEED; TOBACCO; VIRUSES;

BIOPHARMING; DIAGNOSTIC; DIAGNOSTIC ASSAYS; DIRECT USE; FUSION PROTEINS; GENE CONSTRUCTS; MEDICAL DIAGNOSTICS; MOLECULAR PHARMING; RECOMBINANT; TRANSGENEIC PLANTS;

BIOREACTORS;

HORDEUM VULGARE SUBSP. VULGARE; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; NICOTIANA TABACUM; SOLANUM TUBEROSUM;

EID: 0036342361     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026752805494     Document Type: Article

References (40)

1. Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells
2. Potato tubers as a biofactory for recombinant antibodies
3. Upstream sequences regulating legumin gene expression in heterologous transgenic plants
4. Transcription of a maize cDNA in Lotus corniculatus is regulated by T-DNA methylation and transgene copy number
5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding
6. Developmental timing of transgene expression is dosage dependent
7. Construction of recombinant extended single-chain antibody peptide conjugates for use in the diagnosis of HIV-1 and HIV-2
8. Agrobacterium-mediated transformation of New Zealand potato cultivars
9. Maize Adh-I promoter sequences control anaerobic regulation: Addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco
10. Optimisation of scFv antibody production in transgenic plants
11. Molecular farming of pharmaceutical proteins
12. Antibody engineering at the millenniun
13. Expression patterns of vascular specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV resistant plants
14. Commercial production of Avidin from transgenic maize: Characterization of transformant, production, processing, extraction, and purification
15. Affinity maturation of recombinant antibodies using E. coli mutator cells
16. Autologous red cell agglutination assay for HIV-1 antibodies: Simplified test with whole blood
17. Production of a diagnostic monoclonal antibody in perennial alfalfa plants
18. Cleavage of structural proteins during assembly of the head of bacteriophage T4
19. Production of antibodies in transgenic plants
20. Recombinant single-chain antibody peptide conjugates expressed in Escherichia coli for the rapid diagnosis of HIV
21. Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans
22. Sequence and expression of a stem-abundant caffeic acid O-methyltransferase cDNA from perennial ryegrass (Lolium perenne)
23. Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants
24. A C-terminal sequence prevents secretion of luminal ER proteins
25. The retention signal for soluble proteins of the endoplasmic reticulum
26. Transgenic pea seeds as bioreactors for the production of a single-chain Fv fragment (scFV) antibody used in cancer diagnosis and therapy
27. Analysis of a genomic DNA segment carrying the wheat high- molecular-weight (HMW) glutenin B×17subunit and its use as an RFLP marker
28. The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco
29. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies
30. A suite of promoters and terminators for plant biotechnology
31. Agrobacterium tumefaciens-mediated barley transformation
32. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies
33. A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants
34. From farming for food to pharming for pharmaceuticals and other high value proteins in crucifers
35. Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves
36. Improved vectors for Agrobacterium tumefaciens-mediated transformation of monocot plants
37. Studies on the planting distance effect on the open pollination rate in barley
38. A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes
39. Commercial production of aprotinin in transgenic maize seeds