Counterselectable markers: Untapped tools for bacterial genetics and pathogenesis

Infection and Immunity

Volumn 66, Issue 9, 1998, Pages 4011-4017

  Reyrat, Jean Marc ^a,b   Pelicic, Vladimir ^a,c   Gicquel, Brigitte ^a   Rappuoli, Rino ^b  

^a INSTITUT PASTEUR   (France)

^b CHIRON VACCINES   (Italy)

^c UNIVERSITY OF PADOVA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL VACCINE; FUSARIC ACID; LIVE VACCINE; STREPTOMYCIN; SUCROSE;

BACTERIAL GENETICS; BACTERIUM MUTANT; BORDETELLA PERTUSSIS; CHROMOSOME MUTATION; DRUG SENSITIVITY; GENE INSERTION SEQUENCE; GENETIC MARKER; GENETIC SELECTION; HELICOBACTER PYLORI; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PATHOGENESIS; PLASMID; PRIORITY JOURNAL; SHORT SURVEY; TRANSPOSON;

EID: 0031687168     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/.66.9.4011-4017.1998     Document Type: Short Survey

References (57)

1. Azad, A. K., T. D. Sirakova, N. D. Fernandes, and P. E. Kolattukudy. 1997. Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria. J. Biol. Chem. 272:16741-16745.
2. Berg, C. M., and D. E. Berg. 1996. Transposable element tools for microbial genetics, p. 2588-2612. In F. C. Neidhardt, R. Curtis III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology. ASM Press, Washington, D.C
3. Bernard, P., Gabant, P., E. M. Bahassi, and M. Couturier. 1994. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 148:71-74.
4. Blomfield, I. C., V. Vaughn, R. F. Rest, and B. I. Eisenstein. 1991. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature sensitive pSC101 replicon. Mol. Microbiol. 5:1447-1457.
5. Bochner, B. R. 1980. Positive selection for loss of tetracycline resistance. J. Bacteriol. 143:926-933.
6. Burns, D. M., and I. R. Beacham. 1984. Positive selection vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping fragments. Gene 27:323-325.
7. Butterton, J. R., S. A. Boyko, and S. B. Calderwood. 1993. Use of the Vibrio cholerae irgA gene as a locus for insertion and expression of heterologous antigens in cholera vaccine strain. Vaccine 11:1327-1335.
8. Copass, M., G. Grandi, and R. Rappuoli. 1997. Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker. Infect. Immun. 65:1949-1952.
9. Covacci, A., G. C. Kennedy, B. Cormack, R. Rappuoli, and S. Falkow. 1998. From microbial genomics to meta-genomics. Drug Dev. Res. 41:180-192.
10. Dean, D. 1981. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15:99-102.
11. Donnenberg, M. S., and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59:4310-4317.
12. Dumais Pope, C., L. Dhand, and N. P. Cianciotto. 1994. Random mutagenesis of Legionella pneumophila with mini-Tn10. FEMS Microbiol. Lett. 124: 107-112.
13. Gabor-Miklos, G. L., and G. M. Rubin. 1996. The role of the genome project in determining gene function: insights from model organisms. Cell 86:521-529.
14. Gay, P., D. Lecoq, M. Steinmetz, T. Berkelman, and C. I. Kado. 1985. Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J. Bacteriol. 164:918-921.
15. Gay, P., D. Lecoq, M. Steinmetz, E. Ferrari, and J. A. Hoch. 1983. Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli. J. Bacteriol. 153:1424-1431.
16. Greco, D., S. Salmaso, P. Mastrantonio, M. Giuliano, A. E. Tozzi, A. Anemona, M. L. Ciofi degli Atti, A. Giammanco, P. Panei, W. C. Blackwelder, D. L. Klein, and S. G. Wassilak. 1996. A controlled trial of two acellular vaccines and one whole-cell vaccine against pertussis. N. Engl. J. Med. 334:341-348.
17. Hensel, M., and D. W. Holden. 1996. Molecular genetic approaches for the study of virulence in both pathogenic bacteria and fungi. Microbiology 142:1049-1058.
18. Hynes, M. F., J. Quandt, M. P. O'Connell, and A. Pühler. 1989. Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene. Gene 78:111-120.
19. Jäger, W., A. Schäfer, A. Pühler, G. Labes, and W. Wohlleben. 1992. Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans. J. Bacteriol. 174:5462-5465.
20. Kaniga, K., I. Delor, and G. R. Cornells. 1991. A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene 109:137-141.
21. Kast, P. 1994. PKSS - a second-generation general purpose cloning vector for efficient positive selection of recombinant clones. Gene 138:109-114.
22. Knipfer, N. E., and T. E. Shrader. 1997. Inactivation of the 20S proteasome in Mycobacterium smegmatis. Mol. Microbiol. 25:375-383.
23. Kwaga, J. K. P., B. J. Allan, J. V. van den Hurk, H. Seida, and A. A. Potter. 1994. A carAB mutant of avian pathogenic Escherichia coli serogroup O2 is attenuated and effective as a live oral vaccine against collibacillosis in turkeys. Infect. Immun. 62:3766-3772.
24. Lederberg, J. 1951. Streptomycin resistance: a genetically recessive mutation. J. Bacteriol. 61:549-550.
25. Lee, C. A., B. D. Jones, and S. Falkow. 1992. Identification of a Salmonella typhimurium invasion locus by selection for hyperinvasive mutants. Proc. Natl. Acad, Sci. USA 89:1847-1851.
26. Link, A. J., D. Phillips, and G. M. Church. 1997. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179:6228-6237.
27. Maloy, S. R., and W. D. Nunn. 1981. Selection for loss of tetracycline resistance by Escherichia coli. J. Bacteriol. 145:1110-1112,
28. Marsden, M. J., L. M. Vaughan, T. J. Foster, and C. J. Secombes. 1996. A live (ΔaroA) Aeromonas salmonicida vaccine for furunculosis preferentially stimulates T-cell responses relative to B-cell responses in rainbow trout (Oncorhinchus mykiss). Infect. Immun. 64:3863-3869.
29. Martinez-Salazar, J. M., A. N. Palacios, R. Sanchez, A. Caro, and G. Soberón-Chavez. 1993. Genetic stability and xanthan gum production in Xanthomonas campestris pv. campestris NRRL B1459. Mol. Microbiol. 8:1053-1061.
30. McFadden, J. 1996. Recombination in mycobacteria. Mol. Microbiol. 21:205-211.
31. Moxon, R., and R. Rappuoli. 1990. Haemophilus influenzae infections and whooping cough. Lancet i:1324-1329.
32. Murphy, C. K., E. J. Stewart, and J. Beckwith. 1995. A double counter-selection system for the study of null alleles of essential genes in Escherichia coli. Gene 155:1-7.
33. Parsonnet, J., G. D. Friedman, D. P. Vandersteen, Y. Chang, J. H. Vogelman, N. Orentreich, and R. K. Sibley. 1991. Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325:1127-1131.
34. Pavelka, M. S., Jr., and W. R. Jacobs, Jr. 1996. Biosynthesis of diaminopimelate (DAP), the precursor of lysine and a component of the peptidoglycan, is an essential function of Mycobacterium smegmatis. J. Bacteriol. 178:6496-6507.
35. Pelicic, V., M. Jackson, J.-M. Reyrat, W. R. Jacobs, Jr., B. Gicquel, and C. Guilhot. 1997. Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 94:10955-10960.
36. Pelicic, V., J.-M. Reyrat, and B. Gicquel. 1996. Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J. Bacteriol. 178:1197-1199.
37. Pelicic, V., J.-M. Reyrat, and B. Gicquel. 1996. Generation of unmarked directed mutations in mycobacteria, using sucrose countersetectable suicide vectors. Mol. Microbiol. 20:919-925.
38. Pelicic, V., J.-M. Reyrat, and B. Gicquel. 1996. Positive selection of allelic exchange mutants in Mycobacterium bovis BCG. FEMS Microbiol. Lett. 144:161-166.
39. Pierce, J. C., B. Sauer, and N. Sternberg. 1992. A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy. Proc. Natl Acad. Sci. USA 89:2056-2060.
40. Pizza, M., A. Covacci, A. Bartoloni, M. Perugini, L. Nencioni, M. T. de Magistris, L. Villa, D. Nucci, R. Manetti, M. Bugnoli, F. Giovannoni, R. Olivieri, J. T. Barbieri, H. Sato, and R. Rappuoli. 1989. Mutants of pertussis toxin suitable for vaccine development. Science 246:497-500.
41. Rajakumar, K., C. Sasakawa, and B. Adler. 1997. Use of a novel approach, termed island probing, identifies the Shigella flexneri she pathogenicity island which encodes a homolog of immunoglobulin A protease-like family of proteins. Infect. Immun. 65:4606-4614.
42. Rappuoli, R. 1997. Rational design of vaccines. The recombinant pertussis vaccine induces early and long-lasting protection. Nat. Med. 3:374-376.
43. Ried, J. L., and A. Collmer. 1987. An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in Gram-negative bacteria by marker exchange-eviction mutagenesis. Gene 57:239-246.
44. Sabaty, M., and S. Kaplan. 1996. mgpS, a complex regulatory locus involved in the transcriptional control of the puc and puf operons in Rhodobacter sphaeroides. J. Bacteriol. 178:35-45.
45. +: a dominant selectable marker for gene replacement in mycobacteria. Mol. Microbiol. 16:991-1000.
46. 45a.Sansonetti, P. J. Personal communication.
47. Simon, R., B. Hötte, B. Klauke, and B. Kosier. 1991. Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors. J. Bacteriol. 173:1502-1508.
48. Stacey, K, A., and E. Simson. 1965. Improved method for the isolation of thymine-requiring mutants of Escherichia coli. J. Bacteriol. 90:554-555.
49. Steinmetz, M., D. Lecoq, H. Ben Djemia, and P. Gay. 1983. Analyse génétique de sacB, gène de structure d'une enzyme secrétée, la lévane-saccharase de Bacillus subtilis Marburg. Mol. Gen. Genet. 191:138-144.
50. Stibitz, S. 1994. Use of conditionally counterselectable suicide vectors for allelic exchange. Methods Enzymol. 235:458-465.
51. Stibitz, S., W. Black, and S. Falkow. 1986. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50:133-140.
52. Stojiljkovic, I., Z. Trgovcevic, and E. Salaj-Smic. 1991. Tn5-rpsL: a new derivative of transposon Tn5 useful in plasmide curing. Gene 99:101-104.
53. Tomb, J.-F., et al. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388:539-547.
54. Trudel, P., Provost, S., Massie, B., P. Chartrand, and L. Wall. 1996. pGATA: a positive selection vector based on the toxicity of the transcription factor GATA-1 to bacteria. BioTechniques 20:684-693.
55. Wang, G., R. W. Blakesley, D. E. Berg, and C. M. Berg. 1993. pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo. Proc. Natl. Acad. Sci. USA 90:7874-7878.
56. Weston-Hafer, K., and D. E. Berg. 1989. Specificity of deletion events in pBR322. Plasmid 21:251-253.
57. World Health Organization. 1991. Drugs used in mycobacterial infections. World Health Organization, Geneva, Switzerland.