Engineering a polyketide with a longer chain by insertion of an extra module into the erythromycin-producing polyketide synthase

Chemistry and Biology

Volumn 8, Issue 5, 2001, Pages 475-485

  Rowe, Christine J ^a,c   Böhm, Ines U ^a   Thomas, Iain P ^a   Wilkinson, Barrie ^b,c   Rudd, Brian A M ^b   Foster, Graham ^b   Blackaby, Andrew P ^b   Sidebottom, Philip J ^b   Roddis, Ylva ^a,d   Buss, Anthony D ^b,e   Staunton, James ^a   Leadlay, Peter F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c BIOTICA TECHNOLOGY LTD   (United Kingdom)

^d Karo Bio   (Sweden)

^e Singapore Science Park   (Singapore)

Author keywords

Erythromycin; Modular; Module insertion; Octaketide; Polyketide synthase; Rapamycin; Saccharopolyspora erythraea; Skipping

Indexed keywords

ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME ENGINEERING; ERYTHROMYCIN; MULTIENZYME COMPLEX; NATURAL PRODUCT; POLYKETIDE SYNTHASE; RAPAMYCIN; THREE DIMENSIONAL IMAGING;

CYCLOHEXANONES; DISACCHARIDES; ERYTHROMYCIN; MULTIENZYME COMPLEXES; MUTAGENESIS, INSERTIONAL; PROTEIN ENGINEERING; SACCHAROPOLYSPORA;

SACCHAROPOLYSPORA ERYTHRAEA;

EID: 0343371621     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(01)00024-2     Document Type: Article

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