DNA shuffling of subgenomic sequences of subtilisin

Nature Biotechnology

Volumn 17, Issue 9, 1999, Pages 893-896

  Ness, Jon E ^a   Welch, Mark ^a   Giver, Lori ^a   Bueno, Manuel ^b   Cherry, Joel R ^c   Borchert, Torben V ^c   Stemmer, Willem P C ^d   Minshull, Jeremy ^b  

^a Maxygen   (United States)

^b NOVO NORDISK A S   (Denmark)

^c NOVO NORDISK INC   (United States)

^d NONE

Author keywords

Directed evolution; DNA shuffling; Molecular breeding; Savinase; Subtilisin

Indexed keywords

PROTEINASE; SUBTILISIN;

ARTICLE; BACILLUS; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE LIBRARY; GENE SEQUENCE; GENETIC ENGINEERING; GENETIC RECOMBINATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

ENZYME STABILITY; GENE LIBRARY; HEAT; HYDROGEN-ION CONCENTRATION; PEPTIDE FRAGMENTS; PEPTIDE HYDROLASES; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SELECTION (GENETICS); SERINE ENDOPEPTIDASES; SUBTILISINS;

POSIBACTERIA;

EID: 0032885231     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/12884     Document Type: Article

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