Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel "unnatural" natural products

Proceedings of the National Academy of Sciences of the United States of America

Volumn 96, Issue 5, 1999, Pages 1846-1851

  Mcdaniel, Robert ^a   Thamchaipenet, Arinthip ^a,b   Gustafsson, Claes ^a   Fu, Hong ^a   Betlach, Melanie ^a   Betlach, Mary ^a   Ashley, Gary ^a  

^a KOSAN BIOSCIENCES INC   (United States)

^b KASETSART UNIVERSITY   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

AZITHROMYCIN; BACTERIAL ENZYME; CLARITHROMYCIN; ERYTHROMYCIN; ERYTHROMYCIN DERIVATIVE; ERYTHROMYCIN POLYKETIDE SYNTHASE; PEPTIDE LIBRARY; POLYKETIDE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GENETIC MANIPULATION; NONHUMAN; PRIORITY JOURNAL; STREPTOMYCES; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

CATALYTIC DOMAIN; CLONING, MOLECULAR; ERYTHROMYCIN; ESCHERICHIA COLI; GENETIC ENGINEERING; MULTIENZYME COMPLEXES; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; STREPTOMYCES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; STREPTOMYCES; UNCULTURED ACTINOMYCETE;

EID: 0033515090     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.96.5.1846     Document Type: Article

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