A method for allelic replacement in Francisella tularensis

FEMS Microbiology Letters

Volumn 222, Issue 2, 2003, Pages 273-280

  Golovliov, Igor ^a   Sjöstedt, Anders ^a   Mokrievich, Alexander ^b   Pavlov, Vitaly ^b  

^a UMEÅ UNIVERSITY   (Sweden)

^b STATE RESEARCH CENTER FOR APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   (Russian Federation)

Author keywords

23 kDa protein; Allelic replacement; Francisella tularensis; Intracellular survival

Indexed keywords

CHLORAMPHENICOL; PLASMID VECTOR;

ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CONJUGATION; DNA FLANKING REGION; FRANCISELLA TULARENSIS; GENE DELETION; GENE INSERTION; MACROPHAGE; MUTAGENESIS; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOUTHERN BLOTTING; UPREGULATION;

BACILLUS SUBTILIS; FRANCISELLA TULARENSIS; SHIGELLA FLEXNERI;

EID: 0037721461     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00313-6     Document Type: Article

References (32)

1. Sjöstedt, A. (2003) Family XVII. Francisellaceae, Genus I. Francisella. In: Bergey's manual of systematic bacteriology (Brenner, D.J., Ed.). Springer, New York.
2. Sjöstedt A. Virulence determinants and protective antigens of Francisella tularensis. Curr. Opin. Microbiol. 6:2003;1-6.
3. Eigelsbach H.T., Down C.M. Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig. J. Immunol. 87:1961;415-425.
4. Burke D.S. Immunization against tularemia: analysis of the effectiveness of live Francisella tularensis vaccine in prevention of laboratory-acquired tularemia. J. Infect. Dis. 135:1977;55-60.
5. Fortier A.H., Slayter M.V., Ziemba R., Meltzer M.S., Nacy C.A. Live vaccine strain of Francisella tularensis: infection and immunity in mice. Infect. Immun. 59:1991;2922-2928.
6. Tärnvik A. Nature of protective immunity to Francisella tularensis. Rev. Infect. Dis. 11:1989;440-451.
7. Anthony L.S., Gu M.Z., Cowley S.C., Leung W.W., Nano F.E. Transformation and allelic replacement in Francisella spp. J. Gen. Microbiol. 137:1991;2697-2703.
8. Baron G.S., Myltseva S.V., Nano F.E. Electroporation of Francisella tularensis. Methods Mol. Biol. 47:1995;149-154.
9. Pavlov V.M., Mokreivich A.N., Volkovoy K. Cryptic plasmid pFNL10 from Francisella novicida-like F6168: The base of plasmid vectors for Francisella tularensis. FEMS Immunol. Med. Microbiol. 13:1996;253-256.
10. Pomerantsev A.P., Domaradskii I.V., Shishkova N.A. Hybrid plasmid pSKEFT5 assures the transfer of plasmids Sa from Escherichia coli cells into cells of the tularemia microbe vaccine strain cells. Mol. Gen. Mikrobiol. Virusol. 8:1991;13-16.
11. Gray C.G., Cowley S.C., Cheung K.K., Nano F.E. The identification of five genetic loci of Francisella novicida associated with intracellular growth. FEMS Microbiol. Lett. 215:2002;53-56.
12. Cowley S.C., Gray C.J., Nano F.E. Isolation and characterization of Francisella novicida mutants defective in lipopolysaccharide biosynthesis. FEMS Microbiol. Lett. 182:2000;63-67.
13. Baron G.S., Nano F.E. An erythromycin resistance cassette and mini-transposon for constructing transcriptional fusions to cat. Gene. 229:1999;59-65.
14. Gay P., Le Coq D., Steinmetz M., Berkelman T., Kado C.I. Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J. Bacteriol. 164:1985;918-921.
15. Reyrat J.M., Pelicic V., Gicquel B., Rappuoli R. Counterselectable markers: untapped tools for bacterial genetics and pathogenesis. Infect. Immun. 66:1998;4011-4017.
16. Golovliov I., Ericsson M., Sandström G., Tärnvik A., Sjöstedt A. Identification of proteins of Francisella tularensis induced during growth in macrophages and cloning of the gene encoding a prominently induced 23-kilodalton protein. Infect. Immun. 65:1997;2183-2189.
17. Sambrook, J., Fritch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
18. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
19. Watanabe T., Furuse C., Sakaizumi S. Transduction of various R factors by phage P1 in Escherichia coli and by phage P22 in Salmonella typhimurium. J. Bacteriol. 96:1968;1791-1795.
20. Milton D.L., O'Toole R., Horstedt P., Wolf-Watz H. Flagellin A is essential for the virulence of Vibrio anguillarum. J. Bacteriol. 178:1996;1310-1319.
21. Kuoppa K., Forsberg A., Norqvist A. Construction of a reporter plasmid for screening in vivo promoter activity in Francisella tularensis. FEMS Microbiol. Lett. 205:2001;77-81.
22. Ehrlich S.D. DNA cloning in Bacillus subtilis. Proc. Natl. Acad. Sci. USA. 75:1978;1433-1436.
23. Kaniga K., Delor I., Cornelis G.R. A wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene. 109:1991;137-141.
24. Blomfield I.C., Vaughn V., Rest R.F., Eisenstein B.I. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 5:1991;1447-1457.
25. Priefer U.B., Simon R., Puhler A. Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions. J. Bacteriol. 163:1985;324-330.
26. Miller V.L., Mekalanos J.J. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170:1988;2575-2583.
27. Abshire K.Z., Neidhardt F.C. Analysis of proteins synthesized by Salmonella typhimurium during growth within a host macrophage. J. Bacteriol. 175:1993;3734-3743.
28. Telepnev M., Golovliov I., Grundstrom T., Tarnvik A., Sjostedt A. Francisella tularensis inhibits Toll-like receptor-mediated activation of intracellular signalling and secretion of TNF-alpha and IL-1 from murine macrophages. Cell. Microbiol. 5:2003;41-51.
29. Woodcock D.M., Crowther P.J., Doherty J., Jefferson S., DeCruz E., Noyer-Weidner M., Smith S.S., Michael M.Z., Graham M.W. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17:1989;3469-3478.
30. Simon R., Priefer U., Puhler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology. 1:1983;784-791.
31. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.
32. Ehrlich S.D. DNA cloning in Bacillus subtilis. Proc. Natl. Acad. Sci. USA. 75:1978;1433-1436.