Engineering broader specificity into an antibiotic-producing polyketide synthase

Science

Volumn 279, Issue 5348, 1998, Pages 199-202

  Marsden, Andrew F A ^a   Wilkinson, Barrie ^a   Cortés, Jesús ^a   Dunster, Nicholas J ^a   Staunton, James ^a   Leadlay, Peter F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AVERMECTIN; ERYTHROMYCIN; MACROLIDE;

ARTICLE; BACTERIAL INFECTION; BACTERICIDAL ACTIVITY; DRUG SYNTHESIS; ENZYME SPECIFICITY; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; SACCHAROPOLYSPORA;

ANTI-BACTERIAL AGENTS; CARBOXYLIC ACIDS; CLONING, MOLECULAR; ERYTHROMYCIN; FERMENTATION; IVERMECTIN; MULTIENZYME COMPLEXES; PROMOTER REGIONS (GENETICS); PROTEIN ENGINEERING; PROTEIN HYBRIDIZATION; SACCHAROPOLYSPORA; STREPTOMYCES; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); SACCHAROPOLYSPORA; SACCHAROPOLYSPORA ERYTHRAEA;

EID: 0032498205     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5348.199     Document Type: Article

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48. Supported by a project grant from the U.K. Biotechnology and Biological Sciences Research Council. Biotechnology Directorate. We thank P. Skelton and J. B. Lester for assistance with, respectively, mass spectrometry and DNA sequencing; A. L. Cutter for authentic samples of triketide lactones; H. A. I. McArthur and J. Dirlam for helpful discussions; and D. Hopwood and C. Khosla for the kind provision of S. coelicolor CH999 and plasmid pRM5. We also wish to thank Pfizer Central Research for assistance in the scale-up and isolation of some of the novel compounds, and I. Parsons and D. M. Rescek for NMR data.