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A standard HPLC gradient (5% acetonitrile → 45% acetonitrile (0.01% formic acid) over 5 min) was used for all compounds and adjusted for cases that did not show satisfactory separation.
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Preparative HPLC was accomplished using LC gradients based on successful analytical methods for each sample. Criteria for fraction collection was a mixed trigger involving both ELSD intensity and mass identity.
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