Precision-cut liver slices: Characteristics and use for in vitro pharmaco-toxicology

Toxicology

Volumn 153, Issue 1-3, 2000, Pages 221-253

  Lerche Langrand, Carole ^a   Toutain, Herve J ^a  

^a SANOFI   (France)

Author keywords

[No Author keywords available]

Indexed keywords

1 CHLORO 2,4 DINITROBENZENE; 1 NAPHTHOL; 1,1 DICHLORO 2,2,2 TRIFLUOROETHANE; AFLATOXIN; BIPHENYL DERIVATIVE; BRIMONIDINE; BROMOBENZENE; CAFFEINE; CARNITINE; CHOLIC ACID; CLENBUTEROL; CYCLOSPORIN A; DIAZEPAM; ETHIONINE; FENOLDOPAM; GALACTOSAMINE; HALOTHANE; HERNIARIN; LIDOCAINE; ONDANSETRON; PARACETAMOL; PHENYTOIN; PHOSPHORUS; PROTEINASE INHIBITOR; SALBUTAMOL; SALMETEROL; TESTOSTERONE; TOLBUTAMIDE; UNINDEXED DRUG; XENOBIOTIC AGENT;

ANIMAL TISSUE; DRUG DETOXIFICATION; DRUG TRANSFORMATION; HUMAN; HUMAN TISSUE; LIVER METABOLISM; LIVER TOXICITY; NONHUMAN; PRIORITY JOURNAL; REVIEW; TISSUE PREPARATION; TOXICITY TESTING;

EID: 0034676501     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-483X(00)00316-4     Document Type: Article

References (225)

1. Characterization of brimonidine metabolism with rat, rabbit, dog, monkey and human liver fractions and rabbit liver aldehyde oxidase
2. Carbon tetrachloride toxicity in precision-cut rat liver slices
3. 4-induced cytochrome P-450 loss and lipid peroxidation in rat liver slices
4. 4 in rat liver slices
5. The hepatotoxicity of chloroform in precision-cut rat liver slices
6. 3 in precision-cut rat liver slices
7. Glucuronide conjugates of 4-aminobiphenyl and its N-hydroxy metabolites. pH stability and synthesis by human and dog liver
8. The use of tissue slices for pharmaco-toxicology studies
9. Metabolism of a new HIV-1 reverse transcriptase inhibitor, 3-(2- (benzoxazol-2-yl)ethyl)-5-ethyl-6-methylpyridin-2(1H)-one (L-696,229), in rat and liver slices
10. Metabolism of 3-(2-(benzoxazol-2-yl)ethyl)-5-ethyl-6-methylpyridin-2 (1H)-one (L-696,229), an HIV-1 reverse transcriptase inhibitor, by rat liver slices and in humans
11. Metabolites of L-735,524, a potent HIV-1 protease inhibitor, in human urine
12. Metabolism of L-689,502 by rat liver slices to potent HIV-1 protease inhibitors
13. (14C)7-ethoxycoumarin metabolism by precision-cut rat hepatic slices
14. Liver slices in dynamic organ culture. I. An alternative technique for the study of rat hepatic drug metabolism
15. Liver slices in dynamic organ culture. II. An in vitro cellular technique for the study of integrated drug metabolism using human tissue
16. Use of rat and human liver slices for the detection of steroid hormone-induced DNA-adducts in vitro by means of the 32P-postlabeling technique
17. Cell injury and protection in long-term incubation of liver slices after in vivo initiation with paracetamol: Cell injury after in vivo initiation with paracetamol
18. Culture of precision-cut liver slices: Effect of some peroxisome proliferators
19. Use of precision-cut liver slices for studies of unscheduled DNA synthesis
20. Lack of effect of piperonyl butoxide on unscheduled DNA synthesis in precision-cut human liver slices
21. Lack of effect of coumarin on unscheduled DNA synthesis in precision- cut human liver slices
22. Effect of some cooked food mutagens on unscheduled DNA synthesis in cultured precision-cut rat, mouse and human liver slices
23. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence and involvement of mitochondrial electron transport in MTT reduction
24. High yield preparation of isolated rat liver parenchymal cells
25. Caffeine metabolism in liver slices during postnatal development in rats
26. The characterisation of the major metabolite of salmeterol in the dog
27. Precision-cut rat liver slices in dynamic organ culture for structure-toxicity studies
28. Formation and identification of protein adducts to cytosolic proteins in guinea pig liver slices exposed to halothane
29. Glutathione-S-transferase is a target for covalent modification by a halothane reactive intermediate in the guinea pig liver
30. The application of electrospray and neutral-loss mass spectrometry to the identification of the metabolites of S12813 in rat liver slices
31. Mathematical model for chemically induced lipid peroxidation in precision-cut liver slices: Computer simulation and experimental calibration
32. A biologically based pharmacodynamic model for lipid peroxidation stimulated by trichloroethylene in vitro
33. Kinetics of drug metabolism in rat liver slices: IV. Comparison of ethoxycoumarin clearance by liver slices, isolated hepatocytes, and hepatic microsomes from rats pretreated with known modifiers of cytochrome P-450 activity
34. The relative importance of glutathione and metallothionein on protection of hepatotoxicity of menadione in rats
35. Protective roles of metallothionein and glutathione in hepatotoxicity of cadmium
36. Adenine and pyridine nucleotide concentrations and relationships to 2,6- dinitrotoluene metabolism in cultured rat liver slices
37. Viability and function in primary culture of adult hepatocytes from various animal species and human beings after cryopreservation
38. Cocaine hepatotoxicity in cultured liver slices: A species comparison
39. The metabolism and DNA binding of the cooked-food mutagen, 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) in precision-cut rat liver slices
40. Biotransformation of a somatostatin analogue in precision-cut liver and kidney slices from rat, dog and man
41. Cytochrome P450-dependent metabolic pathways and glucuronidation in trout liver slices
42. Rat liver slices: Metabolic changes during short time incubation in glucose-free buffer
43. Hepatic elimination of diazepam: Interactions with albumin, desflurane and sevoflurane
44. Gender aspects of liver slice incubations with N,N,N-triethylene-thiophosphamide (Thio-TEPA) in rats and mice
45. Effect of drug treatment on liver slice function following 72 h hypothermic perfusion
46. Species differences in hepatic glutathione depletion, covalent binding and hepatic necrosis after acetaminophen
47. Cryopreservation of rat and monkey liver slices
48. Organ slices as an in vitro test system for drug metabolism in human liver, lung and kidney
49. Development of a simple incubation system for metabolism studies with precision-cut liver slices
50. A species comparison of tolbutamide metabolism in precision-cut liver slices from rats and dogs. Qualitative and quantitative sex differences
51. Validation of precision-cut liver slices in dynamic organ culture as an in vitro model for studying CYP1A1 and CYP1A2 induction
52. Detection of CYP1A1 protein in human liver and induction by TCDD in precision-cut liver slices incubated in dynamic organ culture
53. Time- and concentration-dependent induction of CYP1A1 and CYP1A2 in precision-cut rat liver slices incubated in dynamic organ culture in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin
54. Determination of 7-hydroxycoumarin and its glucuronide and sulphate conjugates in liver slice incubates by capillary zone electrophoresis
55. The involvement of Kupffer cells in carbon tetrachloride toxicity
56. Quantitative differences in phase I and II metabolism between rat precision-cut liver slices and isolated hepatocytes
57. Vitrification of precision-cut liver slices
58. Short-term maintenance of phase I and II metabolism in precision-cut liver slices in dynamic organ culture
59. Xenobiotic metabolism in rat, dog, and human precision-cut liver slices, freshly isolated hepatocytes, and vitrified precision-cut liver slices
60. Past, present, and future applications of precision-cut liver slices for in vitro xenobiotic metabolism
61. Liver slices as a model in drug metabolism
62. DNA adduct formation of selected sex steroids in human liver slices in vitro
63. Toxicity of chlorobenzenes in cultured rat liver slices
64. Cryopreservation of pig and human liver slices
65. Toxicity of valproic acid in liver slices from Sprague-Dawley rats and domestic pigs
66. In-vitro hepatotoxicity of three dichlorobenzene isomers in human liver slices
67. Valproic acid hepatotoxicity in human liver slices
68. Correlation of metabolism, covalent binding and toxicity for a series of bromobenzene derivatives using rat liver slices in vitro
69. Culture medium composition affects the relative toxicities of chlorobenzenes in rat liver slices and the isolated perfused liver
70. Comparative toxicity of valproic acid and its metabolites in liver slices from adult rats, weanling rats and humans
71. Dynamic organ culture is superior to multiwell plate culture for maintaining precision-cut tissue slices: Optimization of tissue slice culture, part 1
72. Determination of optimal incubation media and suitable slice diameters in precision-cut liver slices: Optimization of tissue slice culture, part 2
73. Comparative metabolism and toxicity of dichlorobenzenes in Sprague- Dawley, Fischer-344 and human liver slices
74. Cold- and cryopreservation of dog liver and kidney slices
75. Use of tissue slices in chemical mixture toxicology and interspecies investigations
76. Milacemide metabolism in rat liver and brain slices by solids NMR
77. Metabolic zonation of the liver: Regulation and implications for liver function
78. Comparison of hepatocyte cultures and liver slices in in vitro toxicity testing
79. Precision-cut guinea pig liver slices as a tool for studying the toxicity of volatile anaesthetics
80. Biotransformation of halothane in guinea pig liver slices
81. Toxicity of halothane in guinea pig liver slices
82. Minimal biotransformation and toxicity of desflurane in guinea pig liver slices
83. Inhibition of protein synthesis and secretion by volatile anesthetics in guinea pig liver slices
84. Effects of halothane and other volatile anaesthetics on protein synthesis and secretion in guinea pig liver slices
85. Sevoflurane is biotransformed by guinea pig liver slices but causes minimal cytotoxicity
86. Dieldrin pretreatment alters (14C)dieldrin and (3H)7,12-dimethylbenz(a)anthracene uptake in rainbow trout liver slices
87. Ethoxycoumarin O-deethylation (ECOD) activity in rat liver slices exposed to beta-naphthoflavone (BNF) in vitro
88. Use of fresh and cryopreserved human liver slices in toxicology with special reference to in vitro induction of cytochrome P450
89. Cytochrome P-450 1A1/1A2 induction, albumin secretion, and histological changes in cultured rat liver slices
90. Cytochrome P450 isoenzyme activities in cultured rat and mouse liver slices
91. Inhibition of hepatic neoglucogenesis and glucose-6-phosphatase by aqueous extract of Bauhinia megalandra leaves
92. Toxicity of ortho-substituted bromobenzenes to isolated hepatocytes: Comparison to in vivo results
93. Liver cell models in in vitro toxicology
94. The N-acetylation of sulfamethazine and p-aminobenzoic acid by human liver slices in dynamic organ culture
95. Metabolism and alkylating activity of thio-TEPA in rat liver slice incubation
96. O6-Alkylguanine DNA alkyltransferase activity in monkey, human and rat liver
97. Metabolism of taxol by human hepatic microsomes and liver slices: Participation of cytochrome P450 3A4 and an unknown P450 enzyme
98. The in vitro metabolism of penclomedine in mouse, rat, and human systems
99. Effects of taurine in precision-cut liver slices exposed to the pyrrolizidine alkaloid retrorsine
100. Stability of phase II conjugation systems in cultured precision-cut rat hepatic slices
101. Structural and functional integrity of precision-cut liver slices in xenobiotic metabolism: A comparison of the dynamic organ and multiwell plate culture procedures
102. Nephrotoxicity testing in vitro
103. Determination of aflatoxin B1 biotransformation and binding to hepatic macromolecules in human precision liver slices
104. Assessment of regional cytochrome P450 activities in rat liver slices using resorufin substrates and fluorescence confocal laser cytometry
105. Metabolism of retigabine (D-23129), a novel anticonvulsant
106. Effect of a perfluorochemical erythrocyte substitute on the in vitro metabolism of lidocaine using rat liver slices
107. Prediction of hepatic clearance from microsomes, hepatocytes, and liver slices
108. Metabolism of taxol by human and rat liver in vitro: A screen for drug interactions and interspecies differences
109. Comparison of human and rat metabolism of molinate in liver microsomes and slices
110. In vitro metabolism of GV150013X by using liver microsomes and liver tissue slices from different species
111. Tissue slice technology for assessing alterations in fish hepatic phase I and phase II XME activity
112. Stereoselective metabolism of fenoldopam and its metabolites in human liver microsomes, cytosol, and slices
113. Identification and induction of cytochrome P450 2E1 in rat Kupffer cells
114. Body size and tissue respiration
115. A new instrument for the rapid preparation of tissue slices
116. Induction of cytochrome P450 1A1 in rat liver slices by 7-ethoxycoumarin and 4-methyl-7-ethoxycoumarin
117. In vitro metabolism of the HIV-1 protease inhibitor ABT-378: Species comparison and metabolite identification
118. Induction of cytochrome P-450-dependent enzyme activities in cultured rat liver slices
119. Effect of peroxisome proliferators and inducers of xenobiotic metabolism on marker enzyme activities in cultured rat liver slices
120. Metabolism of coumarin by precision-cut calf liver slices and calf liver microsomes
121. Use of precision-cut liver slices to evaluate species differences in 2-acetylaminofluorene-induced unscheduled DNA synthesis
122. Induction of cytochrome P450 isoenzymes in cultured precision-cut rat and human liver slices
123. Induction of CYP3A isoforms in cultured precision-cut human liver slices
124. 3,3'-Diindolylmethane induces CYP1A2 in cultured precision-cut human liver slices
125. Inhibition of xenobiotic-induced genotoxicity in cultured precision-cut human and rat liver slices
126. The role of acetylation in benzidine metabolism and DNA adduct formation in dog and rat liver
127. Cytotoxicity of retinoic acid, menadione and aflatoxin B1 in rat liver slices using Netwell inserts as a new culture system
128. Rat liver epithelial cells express functional cytochrome P450 2E1
129. Effect of albumin on the estimation, in vitro, of phenytoin Vmax and Km values: Implications for clinical correlation
130. Uptake and metabolism of 3H-adrenaline and 3H-noradrenaline by isolated hepatocytes and liver slices of the rat
131. In vitro glucuronidation of D-23129, a new anticonvulsant, by human liver microsomes and liver slices
132. Investigation of hepatotoxic interaction using precision cut rat liver slice culture: Steps coward validation, and comparisons to in vivo findings
133. Predictive value of liver slices for metabolism and toxicity in vivo: Use of acetaminophen as a model hepatotoxicant
134. Thermic transition and glycolytic capacity as critical events in the survival of rat liver slices after overnight cold hypoxic preservation
135. Comparison and in vivo relevance of two different in vitro head space metabolic systems: Liver S9 and liver slices
136. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
137. Monooxygenation, cytochrome P4501A1 and P4501A1-mRNA in rat liver slices exposed to beta-naphthoflavone and dexamethasone in vitro
138. Monooxygenation, cytochrome P450-mRNA expression and other functions in precision-cut rat liver slices
139. Characterization of olanzapine (LY170053) in human liver slices by liquid chromatography/tandem mass spectrometry
140. Impairment of TNF-α expression and secretion in primary rat liver cell cultures by acetaminophen treatment
141. Immunohistochemical localization of cytochrome P450 1A1 in precision-cut rat liver slices after in vitro exposure to beta-naphthoflavone
142. Time- and dose-response effects of the mycotoxin, fumonisin B1 on sphingoid base elevations in precision-cut rat liver and kidney slices
143. Mycotoxin-induced elevation of free sphingoid bases in precision-cut rat liver slices: Specificity of the response and structure-activity relationships
144. Toxicity of atractyloside in precision-cut rat and porcine renal and hepatic tissue slices
145. The toxic mechanism and metabolic effects of atractyloside in precision-cut pig kidney and liver slices
146. Optimizing preincubation conditions for precision-cut rat kidney and liver tissue slices: Effect of culture media and antioxidants
147. Assessment of rat liver slices as a suitable model system for studying the simultaneous sulphation and glucuronidation of phenolic xenobiotics
148. Human liver slices express the same lidocaine biotransformation rate as isolated human hepatocytes
149. Comparison of five incubation systems for rat liver slices using functional and viability parameters
150. Effect of cold and warm ischaemia on drug metabolism in isolated hepatocytes and slices from human and monkey liver
151. Effect of human liver source on the functionality of isolated hepatocytes and liver slices
152. Liver slices in in vitro pharmacotoxicology with special reference to the use of human liver tissue
153. Hepatic uptake and metabolism of 2,3,7,8-tetrachlorodibenzo-p-dioxin and 2,3,7,8-tetrachloro-dibenzofuran
154. Identification of 2,6-xylidine as a major lidocaine metabolite in human liver slices
155. Precision-cut tissue slices: Applications in pharmacology and toxicology
156. Benzo(a)pyrene-induced alterations in growth-related gene expression and signaling in precision-cut adult rat liver and kidney slices
157. Chemically induced oxidative stress disrupts the E-cadherin/catenin cell adhesion complex
158. Biotransformation of sevoflurane by rat neonate liver slices
159. Cellular localization of hepatic cytochrome 1B1 expression and its regulation by aromatic hydrocarbons and inflammatory cytokines
160. Dose-response relationships for hepatic aflatoxin B1-DNA adduct formation in the rat in vivo and in vitro: The use of immunoslot blotting for adduct quantitation
161. Inactivation of cytochromes P450 2B protects against cocaine-mediated toxicity in rat liver slices
162. Apparent rates of glutathione turnover in rat tissues
163. A volatile exposure method for precision cut tissue slices: HCFC-123 study
164. Comparison of the metabolism of 7-ethoxycoumarin and coumarin in precision-cut rat liver and lung slices
165. Metabolism of (ring-U-14C) agaritine by precision-cut rat, mouse and human liver and lung slices
166. Comparison of the toxicity of allyl alcohol, coumarin and menadione in precision-cut rat, guinea-pig, cynomolgus monkey and human liver slices
167. Use of precision-cut rat liver slices for studies of xenobiotic metabolism and toxicity: Comparison of the Krumdieck and Brendel tissue slicers
168. Species differences in the metabolism of a potent HIV-1 reverse transcriptase inhibitor L-738, 372. In vivo and in vitro studies in rats, dogs, monkeys, and human
169. Bromobenzene metabolism and hepatic necrosis
170. Mechanism of allyl alcohol-induced necrosis
171. Effect of some indole derivatives on xenobiotic metabolism and xenobiotic-induced toxicity in cultured rat liver slices
172. The in vitro hepatic metabolism of ABT-418, a cholinergic channel activator, in rats, dogs, cynomolgus monkeys, and humans
173. In vitro hepatic metabolism of ABT-418 in chimpanzee (Pan troglodytes). A unique pattern of microsomal flavin-containing monooxygenase-dependent stereoselective N'-oxidation
174. In vitro metabolism of terfenadine by a purified recombinant fusion protein containing cytochrome P4503A4 and NADPH-P450 reductase Comparison to human liver microsomes and precision-cut liver tissue slices
175. In vitro metabolism of theophylline by rat and human liver tissue
176. Studies on the periportal hepatotoxicity of allyl alcohol
177. Beta2-agonist abuse in food producing animals: Use of in vitro liver preparations to assess biotransformation and potential target residues for surveillance
178. The hepatotoxicities of endotoxin and ethanol comparisons in vitro using the precision-cut rat liver slice model
179. Drug metabolism enzymes activities in rat liver epithelial cell lines, hepatocytes and bile duct cells
180. A comparative study of precision cut liver slices, hepatocytes, and liver microsomes from the Wistar rat using metronidazole as a model substance
181. Trout liver slices for metabolism and toxicity studies
182. A dynamic liver culture system: A tool for studying chemical biotransformation and toxicity
183. Dynamic organ culture of precision liver slices for in vitro toxicology
184. In vitro cytotoxicity of allyl alcohol and bromobenzene in a novel organ culture system
185. Precision-cut liver slices: A new in vitro tool in toxicology
186. Microtome for the preparation of tissue slices for metabolic studies of surviving tissues in vitro
187. The metabolism of DuP 753, a nonpeptide angiotensin II receptor antagonist, by rat, monkey, and human liver slices
188. Metabolism of coumarin and 7-ethoxycoumarin by rat, mouse, guinea pig, cynomolgus monkey and human precision-cut liver slices
189. In vitro metabolism of N-(5-chloro-2-methylphenyl)-N'-(2-methylpropyl)thiourea: Species comparison and identification of a novel thiocarbamide-glutathione adduct
190. Hormone- and endotoxin-modulated gene expression of a long-term organ culture system of adult rat liver
191. A species comparison of 14C-labeled 7-ethoxycoumarin metabolism in precision-cut liver slices from guinea pig and dog using a phosphor imaging system
192. Lipid profiling in renal and hepatic tissue slices following exposure to atractyloside
193. Taurine conjugate of 3 alpha,6 beta,7 beta-trihydroxy-5 beta, 22-cholen-24-oic acid (tauro-delta 22-beta-muricholate): The major bile acid in the serum of female rats treated with alpha-naphthylisothiocyanate and its secretion by liver slices
194. Cresol isomers: Comparison of toxic potency in rat liver slices
195. Quinone methide formation from para isomers of methylphenol (cresol), ethylphenol, and isopropylphenol: Relationship to toxicity
196. o-Methoxy-4-alkylphenols that form quinone methides of intermediate reactivity are the most toxic in rat liver slices
197. Metabolism and toxicity of 4-hydroxyphenylacetone in rat liver slices: Comparison with acetaminophen
198. Precision-cut dog renal cortical slices in dynamic organ culture for the study of cisplatin nephrotoxicity
199. Morphological and functional integrity of precision-cut rat liver slices in rotating organ culture and multiwell plate culture: Effects of oxygen tension
200. In vitro metabolic studies of tacrolimus using precision-cut rat and human liver slices
201. Comparison of the in vitro toxicity of dichloroaniline structural isomers
202. Changes in expression of mRNA coding for glutathione S-transferase subunits 1-2 and 7 in cultured rat hepatocytes
203. Alterations of the catalytic activities of drug-metabolizing enzymes in cultures of human liver slices
204. Cyclosporin A metabolism in human liver, kidney, and intestine slices. Comparison to rat and dog slices and human cell lines
205. The biotransformation of the ergot derivative CQA 206-291 in human, dog, and rat liver slice cultures and prediction of in vivo plasma clearance
206. In vivo and in vitro metabolism studies on a class III antiarrhythmic agent
207. Sites of biotransformation for the cyclosporin derivative SDZ IMM 125 using human liver and kidney slices and intestine. Comparison with rat liver slices and cyclosporin A metabolism
208. Xenobiotic metabolism in precision-cut dynamic organ cultured human liver slices
209. Biotransformation of the antiemetic 5-HT3 antagonist tropisetron in liver and kidney slices of human, rat and dog with a comparison to in vivo
210. Human and rat lung biotransformation of cyclosporin A and its derivatives using slices and bronchial epithelial cells
211. Liver slices: An in vitro tool to predict drug biotransformation and to support risk assessment
212. Changes in human liver and kidney slice function related to potential side-effects in the presence of biotransformation of four cyclosporin derivatives-CSA, IMM, OG, and PSC
213. An improved HPLC assay for the assessment of liver slice metabolic viability using 7-ethoxycoumarin
214. The role of oxygen free radicals in isoniazid-induced hepatotoxicity
215. Treatment with aged garlic extract protects against bromobenzene toxicity to precision cut rat liver slices
216. Versuche an uberlebendem Karzinomgewebe
217. Sodium arsenite and heat shock induce stress proteins in precision-cut rat liver slices
218. Interactive toxicity and stress protein expression by vinylidene chloride and monochloroacetate in precision-cut rat liver slices
219. Kinetics of drug metabolism in rat liver slices. Rates of oxidation of ethoxycoumarin and tolbutamide examples of high- and low-clearance compounds
220. Kinetics of drug metabolism in rat liver slices II. Comparison of clearance by liver slices and freshly isolated hepatocytes
221. Metabolite kinetics of ondansetron in rat, comparison of hepatic microsomes, isolated hepatocytes and liver slices with in vivo disposition
222. Kinetics of drug metabolism in rat liver slices III. Relationship between metabolic clearance and slice uptake rate
223. Resistance of precision-cut liver slices to the toxic effects of menadione
224. Evidence that the loss of rat liver cytochrome P450 in vitro is not solely associated with the use of collagenase the loss of cell-cell contacts and/or the absence of an extracellular matrix
225. Preservation of the rate and profile of xenobiotic metabolism in rat hepatocytes stored in liquid nitrogen