[14C]7-ethoxycoumarin metabolism by precision-cut rat hepatic slices

Drug Metabolism and Disposition

Volumn 24, Issue 4, 1996, Pages 383-389

  Ball, Simon E ^a   Thiel, Victoria E ^a   Tio, Cesario O ^a   Lim, Heng Keang ^a   Kao, John ^a   Sisenwine, Samuel F ^a   Scatina, Joann ^a  

^a PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

7 ETHOXYCOUMARIN; CARBON 14; GLUCURONIDE; UMBELLIFERONE; UMBELLIFERONE DERIVATIVE; UMBELLIFERONE SULFATE; UNCLASSIFIED DRUG; 7-ETHOXYCOUMARIN; CARBON; COUMARIN DERIVATIVE;

ANIMAL TISSUE; ARTICLE; BIOTRANSFORMATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIVER METABOLISM; LIVER SLICE; NONHUMAN; PRIORITY JOURNAL; RAT; ANIMAL; COMPARATIVE STUDY; CULTURE MEDIUM; IN VITRO STUDY; LIVER; MALE; MASS SPECTROMETRY; METABOLISM; SPRAGUE DAWLEY RAT; TIME;

ANIMALS; CARBON RADIOISOTOPES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COUMARINS; CULTURE MEDIA; LIVER; MALE; MASS SPECTROMETRY; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

EID: 0029666209     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. P. F. Smith, A. J. Gandolfi, C. L. Krumdieck, C. W. Putnam, C. F. Zukoski, W. M. Davis, and K. Brendel: Dynamic organ culture of precision liver slices for in vitro toxicology. Life Sci. 36, 1367-1375 (1985).
2. I. G. Sipes, R. L. Fisher, P. F. Smith, E. R. Stine, A. J. Gandolfi, and K. Brendel: A dynamic liver culture system: A tool for studying chemical biotransformation and toxicity. Arch. Toxicol. 11, (Suppl.) 20-33 (1987).
3. P. F. Smith, G. Krack, R. L. McKee, D. G. Johnson, A. J. Gandolfi, V. J. Hruby, C. L. Krumdieck, and K. Brendel: Maintenance of adult rat liver slices in dynamic organ culture in vitro. Cell. Devel. Biol. 22, 706-712 (1986).
4. C. L. Krumdieck, J. E. dos Santos, and K.-L. Ho: A new instrument for the rapid preparation of tissue slices. Anal. Biochem. 104, 118-123 (1980).
5. M. G. Miller, J. Beyer, G. L. Hall, L. A. deGraffenried, and P. E. Adams: Predictive value of liver slices for metabolism and toxicity in vitro: Use of acetaminophen as a model hepatotoxicant. Toxicol. Appl. Pharmacol. 122, 108-116 (1993).
6. A. Steensma, J. A. Bearnand, D. G. Walters, R. J. Price, and B. G. Lake: Metabolism of coumarin and 7-ethoxycoumarin by rat, mouse, guinea pig, Cynomolgus monkey, and human precision-cut liver slices. Xenobiotica 24, 893-907 (1994).
7. A. E. M. Vickers, V. Fisher, S. Connors, R. L. Fisher, J.-P. Baldeck, G. Maurer, and K. Brendel: Cyclosporine A metabolism in human liver, kidney, and intestine slices. Comparison to rat and dog slices and human cell lines. Drug Metab. Dispos. 20, 802-809 (1992).
8. P. Dogterom and J. Rothuizen: A species comparison of tolbutamide metabolism in precision-cut liver slices. Drug. Metab. Dispos. 21, 705-709 (1993).
9. B. G. Lake, J. A. Bearnand, A. C. Japenga, A. Renwick, S. Davies, and R J. Price: Induction of cytochrome P450-dependent enzyme activities in cultured rat liver slices. Food Chem. Toxicol. 31, 377-386 (1993).
10. J. A. Bearnand, R. J. Price, M. E. Cunninghame, and B. G. Lake: Culture of precision-cut liver slices: Effect of some peroxisome proliferators. Food Chem. Toxicol. 31, 137-147 (1993).
11. J. Barr, A. J. Weir, K. Brendel, and I. G. Sipes: Liver slices in dynamic organ culture. II. An in vitro cellular technique for the study of integrated drug metabolism using human tissue. Xenobiotica 21, 341-350 (1991).
12. J. Barr, A. J. Weir, K. Brendel, and I. G. Sipes: Liver slices in dynamic organ culture. I. An alternative in vitro technique for the study of rat hepatic drug metabolism. Xenobiotica 21, 331-339 (1991).
13. P. D. Worboys, A. Bradbury, and J. B. Houston: Ethoxycoumarin O-deethylase activity in precision-cut rat liver slices. Br. J. Clin. Pharmacol. 38, 176P (1994).
14. V. Rogiers, L. Anriaenssens, Y. Vandenberghe, E. Gepts, A. Callaerts, and A. Vercruysse: Critical evaluation of 7-ethoxycoumarin O-deethylase activity measurement in intact isolated rat hepatocytes. Xenobiotica 16, 817-826 (1986).
15. J. R. Fry, M. J. Garle, and K. Lal: Differentiation of cytochrome P450 inducers on the basis of alkoxycoumarin O-dealkylase activities. Xenobiotica 22, 211-215 (1992).
16. M. Warren and J. R. Fry: Influence of medium composition on 7-alkoxycoumarin O-dealkylase activities of rat hepatocytes in primary maintenance culture. Xenobiotica 18, 973-981 (1988).
17. A. M. Edwards, M. L. Glistak, C. M. Lucus, and P. A. Wilson: 7-ethoxycoumarin deethylase activity as a convenient measure of liver drug metabolizing enzymes: Regulation in cultured hepatocytes. Biochem. Pharmacol. 33, 1537-1546 (1984).
18. W. Ullrich and P. Weber: The O-dealkylation of 7-ethoxycoumarin by liver microsomes. Hoppeseylers Z. Physiol. Chem. 353, 1171-1177 (1972).
19. W. F. Greenlee and A. Poland: An improved assay of 7-ethoxycoumarin O-deethylase activity. J. Pharmacol. Exp. Ther. 205, 596-605 (1978).
20. S. M. Wishnies, A. R. Parrish, I. G. Sipes, A. J. Gandolfi, C. W. Putnam, C. L. Krumdieck, and K. Brendel: Biotransformation activity in vitrified human liver slices. Cryobiology 28, 216-226 (1991).
21. D. Egan, R. O'Kennedy, E. Moran, D. Cox, E. Prosser, and R. D. Thornes: The pharmacology, metabolism, analysis, and applications of coumarin and coumarin-related compounds. Drug Metab. Rev. 22, 503-529 (1990).
22. J. H. Fentem and J. R. Fry: Metabolism of coumarin by rat, gerbil, and human liver microsomes. Xenobiotica 22, 357-367 (1992).
23. M. M. Bradford: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 (1976).
24. B. Lake: Preparation and characterization of microsomal fractions for studies on xenobiotic metabolism. In "Biochemical Toxicology, A Practical Approach" (K. Snell and B. Mullock, eds.) pp. 183-215, IRL Press, Oxford, 1987.
25. J. Barr, A. J. Weir, K. Brendel, and I.G. Sipes: Liver slices in dynamic organ culture. I. An alternative in vitro technique for the study of rat hepatic drug metabolism. Xenobiotica 21, 331-339 (1991).