Stability of phase II conjugation systems in cultured precision-cut rat hepatic slices

Toxicology in Vitro

Volumn 13, Issue 3, 1999, Pages 459-466

  Hashemi, E ^a   Till, C ^b   Ioannides, C ^b  

^a UNIVERSITY OF SURREY   (United Kingdom)

^b Hoechst Roussel Vet   (United Kingdom)

Author keywords

Conjugation reactions; Epoxide hydrolase; In vitro toxicology; Multiwell system; Tissue slices; Xenobiotic metabolism

Indexed keywords

EPOXIDE HYDROLASE; GLUCURONOSYLTRANSFERASE; GLUTATHIONE REDUCTASE; SULFOTRANSFERASE;

ANIMAL CELL; ARTICLE; CONJUGATION; ENZYME ACTIVITY; LIVER CELL; LIVER MICROSOME METABOLISM; LIVER SLICE; NONHUMAN; PROTEIN CONTENT; RAT; XENOBIOTIC METABOLISM;

EID: 0032866860     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(99)00017-X     Document Type: Article

References (24)

1. Akerboom T.P.H., Sies H. Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods in Enzymology. 7:1981;373-382.
2. Awasthi Y.C., Sharma R., Singhal S.S. Human glutathione S-transferases. International Journal of Biochemistry. 26:1994;295-308.
3. Bach P.H., Vickers A.E.M., Fisher R., Baumann A., Brittebo E., Carlile D.J., Koster H.J., Lake B.G., Salmon F., Sawyer T.W., Skibinski G. The use of tissue slices for pharmacotoxicology studies. ATLA. 24:1996;893-923.
4. 14C]-Ethoxycoumarin metabolism by precision-cut rat hepatic slices. Drug Metabolism and Disposition. 24:1996;383-389.
5. Carlberg I., Mannervik B. Purification and characterisation of the flavoenzyme glutathione reductase from rat liver. Journal of Biological Chemistry. 250:1975;5475-5480.
6. Dansette P.M., DuBois G.C., Jerina D.M. Continuous fluorometric assay of epoxide hydratase activity. Analytical Biochemistry. 97:1979;340-345.
7. DeLeve L.D., Kaplowitz N. Glutathione metabolism and its role in hepatotoxicity. Pharmacology and Therapeutics. 52:1991;287-305.
8. Dogterom P. Development of a single incubation system for metabolism studies with precision-cut liver slices. Drug Metabolism and Disposition. 21:1993;699-704.
9. Ekins S. Past, present, and future applications of precision-cut liver slices for in vitro xenobiotic metabolism. Drug Metabolism Reviews. 28:1996;591-623.
10. Fisher R.L., Hasal S.J., Sanuik J.T., Gandolfi A.J., Brendel K. Determination of optimal incubation media and suitable slice diameters in precision-cut liver slices: optimization of tissue slice culture, Part 2. Toxicological Methods. 5:1995;115-130.
11. Fisher R.L., Shaughnessy P., Jenkins P.M., Austin M.L., Roth G.L., Gandolfi A.J., Brendel K. Dynamic organ culture is superior to multiwell plate culture for maintaining precision-cut tissue slices: optimization of tissue slice culture, Part 1. Toxicological Methods. 5:1995;99-113.
12. Fisher R.L., Smith M.S., Hasal S.J., Hasal K.S., Gandolfi A.J., Brendel K. The use of human lung slices in toxicology. Human and Experimental Toxicology. 13:1994;466-471.
13. Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-transferase, the first enzymic step in mercapturic acid formation. Journal of Biological Chemistry. 249:1974;7130-7139.
14. Hashemi E., Dobrota M., Till C., Ioannides C. Structural and functional integrity of precision-cut liver slices in xenobiotic metabolism: a comparison of the dynamic organ and multiwell plate culture procedures. Xenobiotica. 29:1999;11-25.
15. Ioannides C., Parke D.V. Mechanism of induction of hepatic drug metabolising enzymes by a series of barbiturates. Journal of Pharmacy and Pharmacology. 27:1975;739-749.
16. Lake B.G., Beamand J.A., Japenga A.C., Renwick A., Davies S., Price R.J. Induction of cytochrome P-450-dependent enzyme activities in cultured rat slices. Food and Chemical Toxicology. 31:1993;377-386.
17. Lake B.G., Charzat T., Tredger J.M., Renwick A.B., Beamand J.A., Price R.J. Induction of cytochrome P450 isoenzymes in cultured precision-cut rat and human liver slices. Xenobiotica. 26:1996;297-306.
18. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 193:1951;263-275.
19. Olinga P., Meijer D.K.F., Slooff M.J.H., Groothuis G.M.M. Liver slices in in vitro pharmacotoxicology with special reference to the use of human liver tissue. Toxicology in Vitro. 12:1997;77-100.
20. Olinga P., Groen K., Hof I.H., De Kanter R., Koster H.J., Leeman W.R., Rutten A.A.J.J.L., Van Twillert K., Groothuis G.M.M. Comparison of five incubation systems for rat liver slices using functional and viability parameters. Journal of Pharmacological and Toxicological Methods. 38:1997;59-69.
21. Parrish A.R., Gandolfi A.J., Brendel K. Precision-cut tissue slices: applications in pharmacology and toxicology. Life Sciences. 54:1995;1887-1901.
22. Sekura R.D., Duffel M.W., Jakoby W.B. Aryl sulfotransferases. Methods in Enzymology. 77:1981;197-199.
23. Steensma A., Beamand J.A., Walters D.G., Price R.J., Lake B.G. Metabolism of coumarin and 7-ethoxycoumarin by rat, mouse, guinea pig, Cynomolgus monkey and human precision-cut liver slices. Xenobiotica. 24:1994;893-907.
24. Wisnes A. Studies on the activation in vitro of glucuronyltransferase. Biochimica et Biophysica Acta. 191:1969;279-291.