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Volumn 16, Issue 10, 2015, Pages 24918-24945

High-throughput screening in protein engineering: Recent advances and future perspectives

Author keywords

FACS based screening platforms; High throughput screening; In vitro compartmentalization; Microfluidics based screening platforms; Protein engineering

Indexed keywords

ABELSON KINASE; ACTIVATION INDUCED CYTIDINE DEAMINASE; ARGININE DEIMINASE; ARGININOSUCCINATE SYNTHASE; ARYLSULFATASE; BETA GALACTOSIDASE; BETA GLUCOSIDASE; CYTOPLASM PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; G PROTEIN COUPLED RECEPTOR; HORSERADISH PEROXIDASE; MULTIDRUG RESISTANCE PROTEIN; NEUROTENSIN RECEPTOR; PHOSPHOTRIESTERASE;

EID: 84944729218     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms161024918     Document Type: Review
Times cited : (36)

References (87)
  • 3
    • 84883272014 scopus 로고    scopus 로고
    • Biocatalysis in organic chemistry and biotechnology: Past, present, and future
    • Reetz, M.T. Biocatalysis in organic chemistry and biotechnology: Past, present, and future. J. Am. Chem. Soc. 2013, 135, 12480-12496.
    • (2013) J. Am. Chem. Soc. , vol.135 , pp. 12480-12496
    • Reetz, M.T.1
  • 4
    • 84893488236 scopus 로고    scopus 로고
    • A roadmap to directed enzyme evolution and screening systems for biotechnological applications
    • Martínez, R.; Schwaneberg, U. A roadmap to directed enzyme evolution and screening systems for biotechnological applications. Biol. Res. 2013, 46, 395-405.
    • (2013) Biol. Res. , vol.46 , pp. 395-405
    • Martínez, R.1    Schwaneberg, U.2
  • 6
    • 34247374701 scopus 로고    scopus 로고
    • Selection strategies for improved biocatalysts
    • Boersma, Y.L.; Dröge, M.J.; Quax, W.J. Selection strategies for improved biocatalysts. FEBS J. 2007, 274, 2181-2195.
    • (2007) FEBS J. , vol.274 , pp. 2181-2195
    • Boersma, Y.L.1    Dröge, M.J.2    Quax, W.J.3
  • 7
    • 68049085915 scopus 로고    scopus 로고
    • Directed evolution of enzymes: Library screening strategies
    • Leemhuis, H.; Kelly, R.M.; Dijkhuizen, L. Directed evolution of enzymes: Library screening strategies. IUBMB Life 2009, 61, 222-228.
    • (2009) IUBMB Life , vol.61 , pp. 222-228
    • Leemhuis, H.1    Kelly, R.M.2    Dijkhuizen, L.3
  • 8
    • 0031854986 scopus 로고    scopus 로고
    • Man-made cell-like compartments for molecular evolution
    • Tawfik, D.S.; Griffiths, A.D. Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 1998, 16, 652-656.
    • (1998) Nat. Biotechnol. , vol.16 , pp. 652-656
    • Tawfik, D.S.1    Griffiths, A.D.2
  • 9
    • 33747130345 scopus 로고    scopus 로고
    • Miniaturising the laboratory in emulsion droplets
    • Griffiths, A.D.; Tawfik, D.S. Miniaturising the laboratory in emulsion droplets. Trends Biotechnol. 2006, 24, 395-402.
    • (2006) Trends Biotechnol. , vol.24 , pp. 395-402
    • Griffiths, A.D.1    Tawfik, D.S.2
  • 10
    • 46149085878 scopus 로고    scopus 로고
    • The future of high-throughput screening
    • Mayr, L.M.; Fuerst, P. The future of high-throughput screening. J. Biomol. Screen. 2008, 13, 443-448.
    • (2008) J. Biomol. Screen. , vol.13 , pp. 443-448
    • Mayr, L.M.1    Fuerst, P.2
  • 11
    • 0037131390 scopus 로고    scopus 로고
    • Microfluidic large-scale integration
    • Thorsen, T.; Maerkl, S.J.; Quake, S.R. Microfluidic large-scale integration. Science 2002, 298, 580-584.
    • (2002) Science , vol.298 , pp. 580-584
    • Thorsen, T.1    Maerkl, S.J.2    Quake, S.R.3
  • 12
    • 84873985413 scopus 로고    scopus 로고
    • Flow cytometry-based ultra-high-throughput screening assay for cellulase activity
    • Ostafe, R.; Prodanovic, R.; Commandeur, U.; Fischer, R. Flow cytometry-based ultra-high-throughput screening assay for cellulase activity. Anal. Biochem. 2013, 435, 93-98.
    • (2013) Anal. Biochem. , vol.435 , pp. 93-98
    • Ostafe, R.1    Prodanovic, R.2    Commandeur, U.3    Fischer, R.4
  • 15
    • 84863063178 scopus 로고    scopus 로고
    • Tolerability, pharmacokinetics and pharmacodynamics of CMAB007, a humanized anti-immunoglobulin E monoclonal antibody, in healthy Chinese subjects
    • Zhou, B.; Lin, B.; Li, J.; Qian, W.; Hou, S.; Zhang, D.; Kou, G.; Li, B.; Wang, H.; Chen, Y.; Guo, Y. Tolerability, pharmacokinetics and pharmacodynamics of CMAB007, a humanized anti-immunoglobulin E monoclonal antibody, in healthy Chinese subjects. MAbs 2012, 4, 110-119.
    • (2012) MAbs , vol.4 , pp. 110-119
    • Zhou, B.1    Lin, B.2    Li, J.3    Qian, W.4    Hou, S.5    Zhang, D.6    Kou, G.7    Li, B.8    Wang, H.9    Chen, Y.10    Guo, Y.11
  • 19
    • 34648832245 scopus 로고    scopus 로고
    • Yeast surface display for protein engineering and characterization
    • Gai, S.A.; Wittrup, K.D. Yeast surface display for protein engineering and characterization. Curr. Opin. Struct. Biol. 2007, 17, 467-473.
    • (2007) Curr. Opin. Struct. Biol. , vol.17 , pp. 467-473
    • Gai, S.A.1    Wittrup, K.D.2
  • 20
    • 79960980943 scopus 로고    scopus 로고
    • A general strategy for the evolution of bond-forming enzymes using yeast display
    • Chen, I.; Dorr, B.M.; Liu, D.R. A general strategy for the evolution of bond-forming enzymes using yeast display. Proc. Natl. Acad. Sci. USA 2011, 108, 11399-11404.
    • (2011) Proc. Natl. Acad. Sci. USA , vol.108 , pp. 11399-11404
    • Chen, I.1    Dorr, B.M.2    Liu, D.R.3
  • 21
    • 0035932982 scopus 로고    scopus 로고
    • Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
    • Ilangovan, U.; Ton-That, H.; Iwahara, J.; Schneewind, O.; Clubb, R.T. Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus. Proc. Natl. Acad. Sci. USA 2001, 98, 6056-6061.
    • (2001) Proc. Natl. Acad. Sci. USA , vol.98 , pp. 6056-6061
    • Ilangovan, U.1    Ton-That, H.2    Iwahara, J.3    Schneewind, O.4    Clubb, R.T.5
  • 23
    • 0035711194 scopus 로고    scopus 로고
    • Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency
    • Kapust, R.B.; Tözsér, J.; Fox, J.D.; Anderson, D.E.; Cherry, S.; Copeland, T.D.; Waugh, D.S. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng. 2001, 14, 993-1000.
    • (2001) Protein Eng. , vol.14 , pp. 993-1000
    • Kapust, R.B.1    Tözsér, J.2    Fox, J.D.3    Anderson, D.E.4    Cherry, S.5    Copeland, T.D.6    Waugh, D.S.7
  • 24
    • 84876919211 scopus 로고    scopus 로고
    • Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries
    • Yi, L.; Gebhard, M.C.; Li, Q.; Taft, J.M.; Georgiou, G.; Iverson, B.L. Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries. Proc. Natl. Acad. Sci. USA 2013, 110, 7229-7234.
    • (2013) Proc. Natl. Acad. Sci. USA , vol.110 , pp. 7229-7234
    • Yi, L.1    Gebhard, M.C.2    Li, Q.3    Taft, J.M.4    Georgiou, G.5    Iverson, B.L.6
  • 26
    • 78649692108 scopus 로고    scopus 로고
    • Novel fluorescence-assisted whole-cell assay for engineering and characterization of proteases and their substrates
    • Kostallas, G.; Samuelson, P. Novel fluorescence-assisted whole-cell assay for engineering and characterization of proteases and their substrates. Appl. Environ. Microbiol. 2010, 76, 7500-7508.
    • (2010) Appl. Environ. Microbiol. , vol.76 , pp. 7500-7508
    • Kostallas, G.1    Samuelson, P.2
  • 27
    • 4344658879 scopus 로고    scopus 로고
    • SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags
    • Hersch, G.L.; Baker, T.A.; Sauer, R.T. SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags. Proc. Natl. Acad. Sci. USA 2004, 101, 12136-12141.
    • (2004) Proc. Natl. Acad. Sci. USA , vol.101 , pp. 12136-12141
    • Hersch, G.L.1    Baker, T.A.2    Sauer, R.T.3
  • 28
    • 0037119435 scopus 로고    scopus 로고
    • Genetic analysis of the twin arginine translocator secretion pathway in bacteria
    • DeLisa, M.P.; Samuelson, P.; Palmer, T.; Georgiou, G. Genetic analysis of the twin arginine translocator secretion pathway in bacteria. J. Biol. Chem. 2002, 277, 29825-29831.
    • (2002) J. Biol. Chem. , vol.277 , pp. 29825-29831
    • DeLisa, M.P.1    Samuelson, P.2    Palmer, T.3    Georgiou, G.4
  • 29
    • 0034046020 scopus 로고    scopus 로고
    • The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue
    • Karzai, A.W.; Roche, E.D.; Sauer, R.T. The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue. Nat. Struct. Biol. 2000, 7, 449-455.
    • (2000) Nat. Struct. Biol. , vol.7 , pp. 449-455
    • Karzai, A.W.1    Roche, E.D.2    Sauer, R.T.3
  • 30
    • 79251539925 scopus 로고    scopus 로고
    • Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay
    • Kostallas, G.; Löfdahl, P.-Å.; Samuelson, P. Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay. PLoS ONE 2011, 6, e16136.
    • (2011) PLoS ONE , vol.6
    • Kostallas, G.1    Löfdahl, P.-Å.2    Samuelson, P.3
  • 32
    • 44449126770 scopus 로고    scopus 로고
    • Arginine biosynthesis in Escherichia coli: Experimental perturbation and mathematical modeling
    • Caldara, M.; Dupont, G.; Leroy, F.; Goldbeter, A.; Vuyst, L.D.; Cunin, R. Arginine biosynthesis in Escherichia coli: Experimental perturbation and mathematical modeling. J. Biol. Chem. 2008, 283, 6347-6358.
    • (2008) J. Biol. Chem. , vol.283 , pp. 6347-6358
    • Caldara, M.1    Dupont, G.2    Leroy, F.3    Goldbeter, A.4    Vuyst, L.D.5    Cunin, R.6
  • 33
    • 84934958774 scopus 로고    scopus 로고
    • Conceptual and methodological advances in cell-free directed evolution
    • Dodevski, I.; Markou, G.C.; Sarkar, C.A. Conceptual and methodological advances in cell-free directed evolution. Curr. Opin. Struct. Biol. 2015, 33, 1-7.
    • (2015) Curr. Opin. Struct. Biol. , vol.33 , pp. 1-7
    • Dodevski, I.1    Markou, G.C.2    Sarkar, C.A.3
  • 34
    • 0346728679 scopus 로고    scopus 로고
    • In vitro compartmentalization by double emulsions: Sorting and gene enrichment by fluorescence activated cell sorting
    • Bernath, K.; Hai, M.; Mastrobattista, E.; Griffiths, A.D.; Magdassi, S.; Tawfik, D.S. In vitro compartmentalization by double emulsions: Sorting and gene enrichment by fluorescence activated cell sorting. Anal. Biochem. 2004, 325, 151-157.
    • (2004) Anal. Biochem. , vol.325 , pp. 151-157
    • Bernath, K.1    Hai, M.2    Mastrobattista, E.3    Griffiths, A.D.4    Magdassi, S.5    Tawfik, D.S.6
  • 35
    • 84865466722 scopus 로고    scopus 로고
    • Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions
    • Prodanovic, R.; Ostafe, R.; Blanusa, M.; Schwaneberg, U. Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions. Anal. Bioanal. Chem. 2012, 404, 1439-1447.
    • (2012) Anal. Bioanal. Chem. , vol.404 , pp. 1439-1447
    • Prodanovic, R.1    Ostafe, R.2    Blanusa, M.3    Schwaneberg, U.4
  • 36
    • 79954615462 scopus 로고    scopus 로고
    • A flow cytometry-based screening system for directed evolution of proteases
    • Tu, R.; Martinez, R.; Prodanovic, R.; Klein, M.; Schwaneberg, U. A flow cytometry-based screening system for directed evolution of proteases. J. Biomol. Screen. 2011, 16, 285-294.
    • (2011) J. Biomol. Screen. , vol.16 , pp. 285-294
    • Tu, R.1    Martinez, R.2    Prodanovic, R.3    Klein, M.4    Schwaneberg, U.5
  • 37
    • 84902130440 scopus 로고    scopus 로고
    • Liposome display for in vitro selection and evolution of membrane proteins
    • Fujii, S.; Matsuura, T.; Sunami, T.; Nishikawa, T.; Kazuta, Y.; Yomo, T. Liposome display for in vitro selection and evolution of membrane proteins. Nat. Protoc. 2014, 9, 1578-1591.
    • (2014) Nat. Protoc. , vol.9 , pp. 1578-1591
    • Fujii, S.1    Matsuura, T.2    Sunami, T.3    Nishikawa, T.4    Kazuta, Y.5    Yomo, T.6
  • 38
    • 0038061507 scopus 로고    scopus 로고
    • Production of unilamellar vesicles using an inverted emulsion
    • Pautot, S.; Frisken, B.J.; Weitz, D.A. Production of unilamellar vesicles using an inverted emulsion. Langmuir 2003, 19, 2870-2879.
    • (2003) Langmuir , vol.19 , pp. 2870-2879
    • Pautot, S.1    Frisken, B.J.2    Weitz, D.A.3
  • 39
    • 33846122301 scopus 로고    scopus 로고
    • Spontaneous transfer of phospholipid-coated oil-in-oil and water-in-oil micro-droplets through an oil/water interface
    • Yamada, A.; Yamanaka, T.; Hamada, T.; Hase, M.; Yoshikawa, K.; Baigl, D. Spontaneous transfer of phospholipid-coated oil-in-oil and water-in-oil micro-droplets through an oil/water interface. Langmuir 2006, 22, 9824-9828.
    • (2006) Langmuir , vol.22 , pp. 9824-9828
    • Yamada, A.1    Yamanaka, T.2    Hamada, T.3    Hase, M.4    Yoshikawa, K.5    Baigl, D.6
  • 40
    • 0030447720 scopus 로고    scopus 로고
    • Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore
    • Song, L.; Hobaugh, M.R.; Shustak, C.; Cheley, S.; Bayley, H.; Gouaux, J.E. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore. Science 1996, 274, 1859-1865.
    • (1996) Science , vol.274 , pp. 1859-1865
    • Song, L.1    Hobaugh, M.R.2    Shustak, C.3    Cheley, S.4    Bayley, H.5    Gouaux, J.E.6
  • 41
    • 84863242790 scopus 로고    scopus 로고
    • Functional analysis of membranous Fo-a subunit of F1Fo-ATP synthase by in vitro protein synthesis
    • Kuruma, Y.; Suzuki, T.; Ono, S.; Yoshida, M.; Ueda, T. Functional analysis of membranous Fo-a subunit of F1Fo-ATP synthase by in vitro protein synthesis. Biochem. J. 2012, 442, 631-638.
    • (2012) Biochem. J. , vol.442 , pp. 631-638
    • Kuruma, Y.1    Suzuki, T.2    Ono, S.3    Yoshida, M.4    Ueda, T.5
  • 42
    • 84870367042 scopus 로고    scopus 로고
    • Cell-free protein synthesis of membrane (1,3)-β-D-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs
    • Periasamy, A.; Shadiac, N.; Amalraj, A.; Garajová, S.; Nagarajan, Y.; Waters, S.; Mertens, H.D.T.; Hrmova, M. Cell-free protein synthesis of membrane (1,3)-β-D-glucan (curdlan) synthase: co-translational insertion in liposomes and reconstitution in nanodiscs. Biochim. Biophys. Acta 2013, 1828, 743-757.
    • (2013) Biochim. Biophys. Acta , vol.1828 , pp. 743-757
    • Periasamy, A.1    Shadiac, N.2    Amalraj, A.3    Garajová, S.4    Nagarajan, Y.5    Waters, S.6    Mertens, H.D.T.7    Hrmova, M.8
  • 43
    • 84903137672 scopus 로고    scopus 로고
    • In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume
    • Soga, H.; Fujii, S.; Yomo, T.; Kato, Y.; Watanabe, H.; Matsuura, T. In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume. ACS Synth. Biol. 2014, 3, 372-379.
    • (2014) ACS Synth. Biol. , vol.3 , pp. 372-379
    • Soga, H.1    Fujii, S.2    Yomo, T.3    Kato, Y.4    Watanabe, H.5    Matsuura, T.6
  • 45
    • 84919913491 scopus 로고    scopus 로고
    • A fluorescent hydrogel-based flow cytometry high-throughput screening platform for hydrolytic enzymes
    • Pitzler, C.; Wirtz, G.; Vojcic, L.; Hiltl, S.; Böker, A.; Martinez, R.; Schwaneberg, U. A fluorescent hydrogel-based flow cytometry high-throughput screening platform for hydrolytic enzymes.Chem. Biol. 2014, 21, 1733-1742.
    • (2014) Chem. Biol. , vol.21 , pp. 1733-1742
    • Pitzler, C.1    Wirtz, G.2    Vojcic, L.3    Hiltl, S.4    Böker, A.5    Martinez, R.6    Schwaneberg, U.7
  • 46
    • 0037173305 scopus 로고    scopus 로고
    • Single living cell encapsulation in nano-organized polyelectrolyte shells
    • Diaspro, A.; Silvano, D.; Krol, S.; Cavalleri, O.; Gliozzi, A. Single living cell encapsulation in nano-organized polyelectrolyte shells. Langmuir 2002, 18, 5047-5050.
    • (2002) Langmuir , vol.18 , pp. 5047-5050
    • Diaspro, A.1    Silvano, D.2    Krol, S.3    Cavalleri, O.4    Gliozzi, A.5
  • 47
    • 33750312165 scopus 로고    scopus 로고
    • Biorecognition through layer-by-layer polyelectrolyte assembly: In-situ hybridization on living cells
    • Hillberg, A.L.; Tabrizian, M. Biorecognition through layer-by-layer polyelectrolyte assembly: In-situ hybridization on living cells. Biomacromolecules 2006, 7, 2742-2750.
    • (2006) Biomacromolecules , vol.7 , pp. 2742-2750
    • Hillberg, A.L.1    Tabrizian, M.2
  • 49
    • 18544384019 scopus 로고
    • LXXIII.-Oxidation of tartaric acid in presence of iron
    • Fenton, H.J.H. LXXIII.-Oxidation of tartaric acid in presence of iron. J. Chem. Soc. Trans. 1894, 65, 899-910.
    • (1894) J. Chem. Soc. Trans. , vol.65 , pp. 899-910
    • Fenton, H.J.H.1
  • 50
    • 84929095601 scopus 로고    scopus 로고
    • A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels
    • Lülsdorf, N.; Pitzler, C.; Biggel, M.; Martinez, R.; Vojcic, L.; Schwaneberg, U. A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels. Chem. Commun. 2015, 51, 8679-8682.
    • (2015) Chem. Commun. , vol.51 , pp. 8679-8682
    • Lülsdorf, N.1    Pitzler, C.2    Biggel, M.3    Martinez, R.4    Vojcic, L.5    Schwaneberg, U.6
  • 51
    • 84872867691 scopus 로고    scopus 로고
    • Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells
    • Scott, D.J.; Plückthun, A. Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. J. Mol. Biol. 2013, 425, 662-677.
    • (2013) J. Mol. Biol. , vol.425 , pp. 662-677
    • Scott, D.J.1    Plückthun, A.2
  • 52
    • 84922792125 scopus 로고    scopus 로고
    • Rapid directed evolution of stabilized proteins with cellular high-throughput encapsulation solubilization and screening (CHESS)
    • Yong, K.J.; Scott, D.J. Rapid directed evolution of stabilized proteins with cellular high-throughput encapsulation solubilization and screening (CHESS). Biotechnol. Bioeng. 2015, 112, 438-446.
    • (2015) Biotechnol. Bioeng. , vol.112 , pp. 438-446
    • Yong, K.J.1    Scott, D.J.2
  • 53
    • 84865068808 scopus 로고    scopus 로고
    • Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution
    • Schlinkmann, K.M.; Hillenbrand, M.; Rittner, A.; Künz, M.; Strohner, R.; Plückthun, A. Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. J. Mol. Biol. 2012, 422, 414-428.
    • (2012) J. Mol. Biol. , vol.422 , pp. 414-428
    • Schlinkmann, K.M.1    Hillenbrand, M.2    Rittner, A.3    Künz, M.4    Strohner, R.5    Plückthun, A.6
  • 54
    • 84905615352 scopus 로고    scopus 로고
    • Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies
    • Scott, D.J.; Kummer, L.; Egloff, P.; Bathgate, R.A.D.; Plückthun, A. Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies. BBA Biomembr. 2014, 1838, 2817-2824.
    • (2014) BBA Biomembr. , vol.1838 , pp. 2817-2824
    • Scott, D.J.1    Kummer, L.2    Egloff, P.3    Bathgate, R.A.D.4    Plückthun, A.5
  • 56
    • 77949285840 scopus 로고    scopus 로고
    • Manipulation of droplets in microfluidic systems
    • Yang, C.-G.; Xu, Z.-R.; Wang, J.-H. Manipulation of droplets in microfluidic systems. TrAC Trends Anal. Chem. 2010, 29, 141-157.
    • (2010) TrAC Trends Anal. Chem. , vol.29 , pp. 141-157
    • Yang, C.-G.1    Xu, Z.-R.2    Wang, J.-H.3
  • 57
    • 84900653348 scopus 로고    scopus 로고
    • One in a million: Flow cytometric sorting of single cell-lysate assays in monodisperse picoliter double emulsion droplets for directed evolution
    • Zinchenko, A.; Devenish, S.R.A.; Kintses, B.; Colin, P.-Y.; Fischlechner, M.; Hollfelder, F. One in a million: Flow cytometric sorting of single cell-lysate assays in monodisperse picoliter double emulsion droplets for directed evolution. Anal. Chem. 2014, 86, 2526-2533.
    • (2014) Anal. Chem. , vol.86 , pp. 2526-2533
    • Zinchenko, A.1    Devenish, S.R.A.2    Kintses, B.3    Colin, P.-Y.4    Fischlechner, M.5    Hollfelder, F.6
  • 58
    • 0032156886 scopus 로고    scopus 로고
    • Pore morphology influence on catalytic turn-over for enzyme activated porous silicon matrices
    • Drott, J.; Rosengren, L.; Lindström, K.; Laurell, T. Pore morphology influence on catalytic turn-over for enzyme activated porous silicon matrices. Thin Solid Films 1998, 330, 161-166.
    • (1998) Thin Solid Films , vol.330 , pp. 161-166
    • Drott, J.1    Rosengren, L.2    Lindström, K.3    Laurell, T.4
  • 59
    • 84901930983 scopus 로고    scopus 로고
    • CotA laccase: High-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics
    • Beneyton, T.; Coldren, F.; Baret, J.-C.; Griffiths, A.D.; Taly, V. CotA laccase: High-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics. Analyst 2014, 139, 3314-3323.
    • (2014) Analyst , vol.139 , pp. 3314-3323
    • Beneyton, T.1    Coldren, F.2    Baret, J.-C.3    Griffiths, A.D.4    Taly, V.5
  • 61
    • 0037455351 scopus 로고    scopus 로고
    • Formation of dispersions using "flow focusing" in microchannels
    • Anna, S.L.; Bontoux, N.; Stone, H.A. Formation of dispersions using "flow focusing" in microchannels. Appl. Phys. Lett. 2003, 82, 364-366.
    • (2003) Appl. Phys. Lett. , vol.82 , pp. 364-366
    • Anna, S.L.1    Bontoux, N.2    Stone, H.A.3
  • 63
    • 0344875161 scopus 로고    scopus 로고
    • Millisecond kinetics on a microfluidic chip using nanoliters of reagents
    • Song, H.; Ismagilov, R.F. Millisecond kinetics on a microfluidic chip using nanoliters of reagents. J. Am. Chem. Soc. 2003, 125, 14613-14619.
    • (2003) J. Am. Chem. Soc. , vol.125 , pp. 14613-14619
    • Song, H.1    Ismagilov, R.F.2
  • 68
    • 84857165856 scopus 로고    scopus 로고
    • A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution
    • Fallah-Araghi, A.; Baret, J.-C.; Ryckelynck, M.; Griffiths, A.D. A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution. Lab Chip 2012, 12, 882-891.
    • (2012) Lab Chip , vol.12 , pp. 882-891
    • Fallah-Araghi, A.1    Baret, J.-C.2    Ryckelynck, M.3    Griffiths, A.D.4
  • 71
    • 84931291559 scopus 로고    scopus 로고
    • Dissecting enzyme function with microfluidic-based deep mutational scanning
    • Romero, P.A.; Tran, T.M.; Abate, A.R. Dissecting enzyme function with microfluidic-based deep mutational scanning. Proc. Natl. Acad. Sci. USA 2015, doi:10.1073/pnas.1422285112.
    • (2015) Proc. Natl. Acad. Sci. USA
    • Romero, P.A.1    Tran, T.M.2    Abate, A.R.3
  • 72
    • 84858026557 scopus 로고    scopus 로고
    • A simple microfluidic device for fabrication of double emulsion droplets and polymer microcapsules
    • Nurumbetov, G.; Ballard, N.; Bon, S.A.F. A simple microfluidic device for fabrication of double emulsion droplets and polymer microcapsules. Polym. Chem. 2012, 3, 1043-1047.
    • (2012) Polym. Chem. , vol.3 , pp. 1043-1047
    • Nurumbetov, G.1    Ballard, N.2    Bon, S.A.F.3
  • 73
  • 75
    • 84905217368 scopus 로고    scopus 로고
    • Deep mutational scanning: A new style of protein science
    • Fowler, D.M.; Fields, S. Deep mutational scanning: A new style of protein science. Nat. Methods 2014, 11, 801-807.
    • (2014) Nat. Methods , vol.11 , pp. 801-807
    • Fowler, D.M.1    Fields, S.2
  • 76
    • 33748307400 scopus 로고    scopus 로고
    • Cell-free translation systems for protein engineering
    • Shimizu, Y.; Kuruma, Y.; Ying, B.-W.; Umekage, S.; Ueda, T. Cell-free translation systems for protein engineering. FEBS J. 2006, 273, 4133-4140.
    • (2006) FEBS J. , vol.273 , pp. 4133-4140
    • Shimizu, Y.1    Kuruma, Y.2    Ying, B.-W.3    Umekage, S.4    Ueda, T.5
  • 78
    • 19144366814 scopus 로고    scopus 로고
    • Metagenomics and industrial applications
    • Lorenz, P.; Eck, J. Metagenomics and industrial applications. Nat. Rev. Microbiol. 2005, 3, 510-516.
    • (2005) Nat. Rev. Microbiol. , vol.3 , pp. 510-516
    • Lorenz, P.1    Eck, J.2
  • 79
    • 84919883694 scopus 로고    scopus 로고
    • Droplet-based microfluidics platform for ultra-high-throughput bioprospecting of cellulolytic microorganisms
    • Najah, M.; Calbrix, R.; Mahendra-Wijaya, I.P.; Beneyton, T.; Griffiths, A.D.; Drevelle, A. Droplet-based microfluidics platform for ultra-high-throughput bioprospecting of cellulolytic microorganisms. Chem. Biol. 2014, 21, 1722-1732.
    • (2014) Chem. Biol. , vol.21 , pp. 1722-1732
    • Najah, M.1    Calbrix, R.2    Mahendra-Wijaya, I.P.3    Beneyton, T.4    Griffiths, A.D.5    Drevelle, A.6
  • 82
    • 84887026071 scopus 로고    scopus 로고
    • Recent advances in rational approaches for enzyme engineering
    • Steiner, K.; Schwab, H. Recent advances in rational approaches for enzyme engineering. Comput. Struct. Biotechnol. J. 2012, 2, e201209010.
    • (2012) Comput. Struct. Biotechnol. J. , vol.2
    • Steiner, K.1    Schwab, H.2
  • 84
    • 84856734748 scopus 로고    scopus 로고
    • Self-regulated, droplet-based sample chopper for microfluidic absorbance detection
    • Deal, K.S.; Easley, C.J. Self-regulated, droplet-based sample chopper for microfluidic absorbance detection. Anal. Chem. 2012, 84, 1510-1516.
    • (2012) Anal. Chem. , vol.84 , pp. 1510-1516
    • Deal, K.S.1    Easley, C.J.2
  • 85
    • 79954554233 scopus 로고    scopus 로고
    • Ultrafast surface enhanced resonance Raman scattering detection in droplet-based microfluidic systems
    • Cecchini, M.P.; Hong, J.; Lim, C.; Choo, J.; Albrecht, T.; deMello, A.J.; Edel, J.B. Ultrafast surface enhanced resonance Raman scattering detection in droplet-based microfluidic systems. Anal. Chem. 2011, 83, 3076-3081.
    • (2011) Anal. Chem. , vol.83 , pp. 3076-3081
    • Cecchini, M.P.1    Hong, J.2    Lim, C.3    Choo, J.4    Albrecht, T.5    deMello, A.J.6    Edel, J.B.7
  • 86
    • 84862189816 scopus 로고    scopus 로고
    • On-chip continuous monitoring of motile microorganisms on an ePetri platform
    • Lee, S.A.; Zheng, G.; Mukherjee, N.; Yang, C. On-chip continuous monitoring of motile microorganisms on an ePetri platform. Lab Chip 2012, 12, 2385-2390.
    • (2012) Lab Chip , vol.12 , pp. 2385-2390
    • Lee, S.A.1    Zheng, G.2    Mukherjee, N.3    Yang, C.4
  • 87
    • 84915749162 scopus 로고    scopus 로고
    • Accurate microfluidic sorting of droplets at 30 kHz
    • Sciambi, A.; Abate, A.R. Accurate microfluidic sorting of droplets at 30 kHz. Lab Chip 2014, 15, 47-51.
    • (2014) Lab Chip , vol.15 , pp. 47-51
    • Sciambi, A.1    Abate, A.R.2


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