Metagenomic cellulases highly tolerant towards the presence of ionic liquids - Linking thermostability and halotolerance

Applied Microbiology and Biotechnology

Volumn 95, Issue 1, 2012, Pages 135-146

  Ilmberger, Nele ^a   Meske, Diana ^b   Juergensen, Julia ^a   Schulte, Michael ^c   Barthen, Peter ^c   Rabausch, Ulrich ^a   Angelov, Angel ^d   Mientus, Markus ^d   Liebl, Wolfgang ^d   Schmitz, Ruth A ^b   Streit, Wolfgang R ^a  

^a UNIVERSITY OF HAMBURG   (Germany)

^b UNIVERSITY OF KIEL   (Germany)

^c MERCK KGAA   (Germany)

^d Technischen Universtät München   (Germany)

Author keywords

Cellulase; Halotolerance; Ionic liquid; Metagenomics; Thermostability

Indexed keywords

BACTERIAL ENZYMES; BIOGAS PLANTS; CELLULASE; ENRICHMENT CULTURE; HALOTOLERANCE; HALOTOLERANT; HIGH ACTIVITY; KAMCHATKA; METAGENOMES; METAGENOMIC LIBRARIES; METAGENOMICS; MODERATELY THERMOPHILIC; REACTION MEDIUM; RELATIVE ACTIVITIES; RENEWABLE RESOURCE; THERMOPHILICITY; THERMOSTABILITY; THERMOSTABLE ENZYMES; THERMOTOGA MARITIMA;

BIOGAS; CELLULOSE; ENZYMATIC HYDROLYSIS; ETHANOL; SODIUM CHLORIDE; WOOD;

IONIC LIQUIDS;

BIOGAS; CELLULASE; CELLULOSE; IONIC LIQUID;

AQUEOUS SOLUTION; BACTERIUM; BIOTECHNOLOGY; BIVALVE; CELLULOSE; ENZYME ACTIVITY; FECES; GENOMICS; HYDROLYSIS; MICROBIAL ACTIVITY; RENEWABLE RESOURCE; SALINITY TOLERANCE; TEMPERATURE TOLERANCE; THERMOPHILIC BACTERIUM;

ANIMAL SHELL; ARTICLE; ENRICHMENT CULTURE; HYDROLYSIS; METAGENOMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; SALT TOLERANCE; SEQUENCE ANALYSIS; THERMOSTABILITY; THERMOTOGA;

ANIMALS; CELLULASES; ENZYME STABILITY; GENE LIBRARY; HOT TEMPERATURE; HYDROLYSIS; IONIC LIQUIDS; METAGENOME; METAGENOMICS; SODIUM CHLORIDE;

BIOGAS; CELLULASE; CELLULOSE; ENZYMATIC ACTIVITY; ENZYMOLYSIS; ETHANOL; IONIC MOBILITY; SODIUM CHLORIDE; THERMAL STABILITY; WOOD;

BACTERIA (MICROORGANISMS); TEREDO NAVALIS; THERMOTOGA MARITIMA;

EID: 84862018347     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3732-2     Document Type: Article

References (36)

1. Adsul MG, Terwadkar AP, Varma AJ, Gokhale D (2009) Cellulases from Penicillium janthinellum mutants: solid-state production and their stability in ionic liquids. BioRes 4:1670-1681
2. Anbarasan S, Janis J, Paloheimo M, Laitaoja M, Vuolanto M, Karimaki J, Vainiotalo P, Leisola M, Turunen O (2010) Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa. Appl Environ Microbiol 76:356-360
3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
4. Bridgwater AV, Peacocke GVC (2000) Fast pyrolysis processes for biomass. Renew Sustain Energ Rev 4:1-73 (Pubitemid 30534052)
5. Constantinescu D, Weingärtner H, Herrmann C (2007) Protein denaturation by ionic liquids and the Hofmeister series: a case study of aqueous solutions of ribonuclease A. Angew Chem Int Ed 46:8887-8889 (Pubitemid 350207949)
6. Datta S, Holmes B, Park JI, Chen Z, Dibble DC, Hadi M, Blanch HW, Simmons BA, Sapra A (2010) Ionic liquid tolerant hyperthermophilic cellulases for biomass pretreatment and hydrolysis. Green Chem 12:338-345
7. Engel P, Mladenov R, Wulfhorst H, Jäger G, Spiess AC (2010) Point by point analysis: how ionic liquid affects the enzymatic hydrolysis of native and modified cellulose. Green Chem 12:1959-1966
8. Heinze T, Schwikal K, Barthel S (2005) Ionic liquids as reaction medium in cellulose functionalization. Macromol Biosci 5:520-525 (Pubitemid 40933990)
9. Hofmeister F (1888) Zur Lehre von der Wirkung der Salze. Zweite Mittheilung. Arch Exp Pathol Pharmakol 24:247-260
10. Ilmberger N, Streit WR (2010) Screening for cellulase-encoding clones in metagenomic libraries. Methods Mol Biol 668:177-188
11. Kataeva I, Li XL, Chen H, Choi SK, Ljungdahl LG (1999) Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination. J Bacteriol 181:5288-5295 (Pubitemid 29416348)
12. Kataeva IA, Uversky VN, Brewer JM, Schubot F, Rose JP, Wang BC, Ljungdahl LG (2004) Interactions between immunoglobulin-like and catalytic modules in Clostridium thermocellum cellulosomal cellobiohydrolase CbhA. Protein Eng Des Sel 17:759-762
13. Kaushik JK, Ogasahara K, Yutani K (2002) The unusually slow relaxation kinetics of the folding-unfolding of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus. J Mol Biol 316:991-1003 (Pubitemid 34722136)
14. Kunz W, Henle J, Ninham BW (2004) 'Zur Lehre von der Wirkung der Salze' (about the science of the effect of salts): Franz Hofmeister's historical papers. Curr Opin Colloid Interface Sci 9:19-37 (Pubitemid 39194131)
15. Liang C, Xue Y, Fioroni M, Rodriguez-Ropero F, Zhou C, Schwaneberg U, Ma Y (2011) Cloning and characterization of a thermostable and halo-tolerant endoglucanase from Thermoanaerobacter tengcongensis MB4. Appl Microbiol Biotechnol 89:315-325
16. Liebl W, Ruile P, Bronnenmeier K, Riedel K, Lottspeich F, Greif I (1996) Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterization of the recombinant enzymes. Microbiology 142:2533-2542 (Pubitemid 26333786)
17. Maruyama T, Nagasawa S, Goto M (2002) Poly(ethylene glycol)- lipase complex that is catalytically active for alcoholysis reactions in ionic liquids. Biotechnol Lett 24:1341-1345 (Pubitemid 35000932)
18. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426-428
19. Ogasahara K, Nakamura M, Nakura S, Tsunasawa S, Kato I, Yoshimoto T, Yutani K (1998) The unusually slow unfolding rate causes the high stability of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus: equilibrium and kinetic studies of guanidine hydrochloride-induced unfolding and refolding. Biochemistry 37:17537-17544 (Pubitemid 29013755)
20. Park S, Kazlauskas RJ (2001) Improved preparation and use of roomtemperature ionic liquids in lipase-catalyzed enantio- and regioselective acylations. J Org Chem 66:8395-8401 (Pubitemid 34002932)
21. Pereira JH, Sapra R, Volponi JV, Kozina CL, Simmons B, Adams PD (2009) Structure of endoglucanase Cel9A from the thermoacidophilic Alicyclobacillus acidocaldarius. Acta Crystallogr D Biol Crystallogr 65:744-750
22. Pottkämper J, Barthen P, Ilmberger N, Schwaneberg U, Schenk A, Schulte M, Ignatiev N, Streit WR (2009) Applying metagenomics for the identification of bacterial cellulases that are stable in ionic liquids. Green Chem 11:957-965
23. Remsing RC, Swatloski RP, Rogers RD, Moyna G (2006) Mechanism of cellulose dissolution in the ionic liquid 1-n-butyl-3-methylimidazolium chloride: a 13C and 35/37Cl NMR relaxation study on model systems. Chem Commun (Camb) 1271-1273
24. Sambrook J, Russel DW (2001) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, New York
25. Sánchez C (2009) Lignocellulosic residues: biodegradation and bioconversion by fungi. Biotechnol Adv 27:185-194
26. Shill K, Padmanabhan S, Xin Q, Prausnitz JM, Clark DS, Blanch HW (2011) Ionic liquid pretreatment of cellulosic biomass: enzymatic hydrolysis and ionic liquid recycle. Biotechnol Bioeng 108:511-520
27. Simon C, Daniel R (2010) Construction of small-insert and large-insert metagenomic libraries. Methods Mol Biol 668:39-50
28. Suen G, Weimer PJ, Stevenson DM, Aylward FO, Boyum J, Deneke J, Drinkwater C, Ivanova NN, Mikhailova N, Chertkov O, Goodwin LA, Currie CR, Mead D, Brumm PJ (2011) The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. PLoS ONE 6:e18814
29. Swatloski RP, Spear SK, Holbrey JD, Rogers RD (2002) Dissolution of cellulose [correction of cellose] with ionic liquids. J Am Chem Soc 124:4974-4975 (Pubitemid 34495887)
30. Unsworth LD, van der Oost J, Koutsopoulos S (2007) Hyperthermophilic enzymes-stability, activity and implementation strategies for high temperature applications. FEBS Journal 274:4044-4056 (Pubitemid 47228643)
31. Vazquez-Figueroa E, Yeh V, Broering JM, Chaparro-Riggers JF, Bommarius AS (2008) Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media. Protein Eng Des Sel 21:673-680
32. Weiland N, Löscher C, Metzger R, Schmitz R (2010) Construction and screening of marine metagenomic libraries. Methods Mol Biol 668:51-65
33. Wu J, Zhang J, Zhang H, He J, Ren Q, Guo M (2004) Homogeneous acetylation of cellulose in a new ionic liquid. Biomacromolecules 5:266-268 (Pubitemid 38404459)
34. Yang Z (2009) Hofmeister effects: an explanation for the impact of ionic liquids on biocatalysis. J Biotechnol 144:12-22
35. Zhao H (2006) Are ionic liquids kosmotropic or chaotropic? An evaluation of available thermodynamic parameters for quantifying the ion kosmotropicity of ionic liquids. J Chem Technol Biotechnol 81:877-891
36. Zverlov VV, Velikodvorskaya GV, Schwarz WH, Bronnenmeier K, Kellermann J, Staudenbauer WL (1998) Multidomain structure and cellulosomal localization of the Clostridium thermocellum cellobiohydrolase CbhA. J Bacteriol 180:3091-3099 (Pubitemid 28285021)