-
1
-
-
0036302814
-
Protease degradomics: A new challenge for proteomics
-
López-Otín C, Overall C (2002) Protease degradomics: A new challenge for proteomics. Nat Rev Mol Cell Biol 3: 509-519.
-
(2002)
Nat Rev Mol Cell Biol
, vol.3
, pp. 509-519
-
-
López-Otín, C.1
Overall, C.2
-
2
-
-
52949151513
-
Protease proteomics: Revealing protease in vivo functions using systems biology approaches
-
Doucet A, Overall C (2009) Protease proteomics: Revealing protease in vivo functions using systems biology approaches. Mol Aspects Med 29: 339-358.
-
(2009)
Mol Aspects Med
, vol.29
, pp. 339-358
-
-
Doucet, A.1
Overall, C.2
-
3
-
-
18244414803
-
Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor
-
Duesbery NS, Webb CP, Leppla SH, Gordon VM, Klimpel KR, et al. (1998) Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science 280: 734-737.
-
(1998)
Science
, vol.280
, pp. 734-737
-
-
Duesbery, N.S.1
Webb, C.P.2
Leppla, S.H.3
Gordon, V.M.4
Klimpel, K.R.5
-
4
-
-
33644545381
-
Tumour microenvironment - opinion: Validating matrix metalloproteinases as drug targets and anti-targets for cancer therapy
-
Overall C, Kleifeld O (2006) Tumour microenvironment - opinion: validating matrix metalloproteinases as drug targets and anti-targets for cancer therapy. Nat Rev Cancer 6: 227-239.
-
(2006)
Nat Rev Cancer
, vol.6
, pp. 227-239
-
-
Overall, C.1
Kleifeld, O.2
-
5
-
-
33748308883
-
Targeting proteases: Successes, failures and future prospects
-
Turk B (2006) Targeting proteases: successes, failures and future prospects. Nature reviews Drug discovery 5: 785-799.
-
(2006)
Nature reviews Drug discovery
, vol.5
, pp. 785-799
-
-
Turk, B.1
-
6
-
-
12344293248
-
Papa's got a brand new tag: Advances in identification of proteases and their substrates
-
Marnett AB, Craik CS (2005) Papa's got a brand new tag: advances in identification of proteases and their substrates. Trends Biotechnol 23: 59-64.
-
(2005)
Trends Biotechnol
, vol.23
, pp. 59-64
-
-
Marnett, A.B.1
Craik, C.S.2
-
7
-
-
33847276695
-
In search of partners: Linking extracellular proteases to substrates
-
Overall C, Blobel CP (2007) In search of partners: linking extracellular proteases to substrates. Nat Rev Mol Cell Biol 8: 245-257.
-
(2007)
Nat Rev Mol Cell Biol
, vol.8
, pp. 245-257
-
-
Overall, C.1
Blobel, C.P.2
-
8
-
-
0027316004
-
Substrate phage: Selection of protease substrates by monovalent phage display
-
Matthews DJ, Wells JA (1993) Substrate phage: selection of protease substrates by monovalent phage display. Science 260: 1113-1117.
-
(1993)
Science
, vol.260
, pp. 1113-1117
-
-
Matthews, D.J.1
Wells, J.A.2
-
9
-
-
1842864784
-
Phage display substrate: A blind method for determining protease specificity
-
Deperthes D (2002) Phage display substrate: a blind method for determining protease specificity. Biological Chemistry 383: 1107-1112.
-
(2002)
Biological Chemistry
, vol.383
, pp. 1107-1112
-
-
Deperthes, D.1
-
10
-
-
33646726556
-
Protease specificity determination by using cellular libraries of peptide substrates (CLiPS)
-
Boulware KT, Daugherty PS (2006) Protease specificity determination by using cellular libraries of peptide substrates (CLiPS). Proc Natl Acad Sci USA 103: 7583-7588.
-
(2006)
Proc Natl Acad Sci USA
, vol.103
, pp. 7583-7588
-
-
Boulware, K.T.1
Daugherty, P.S.2
-
11
-
-
0034608931
-
Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries
-
Harris JL, Backes BJ, Leonetti F, Mahrus S, Ellman JA, et al. (2000) Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries. Proc Natl Acad Sci USA 97: 7754-7759.
-
(2000)
Proc Natl Acad Sci USA
, vol.97
, pp. 7754-7759
-
-
Harris, J.L.1
Backes, B.J.2
Leonetti, F.3
Mahrus, S.4
Ellman, J.A.5
-
12
-
-
0030849093
-
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis
-
Thornberry NA, Rano TA, Peterson EP, Rasper DM, Timkey T, et al. (1997) A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J Biol Chem 272: 17907-17911.
-
(1997)
J Biol Chem
, vol.272
, pp. 17907-17911
-
-
Thornberry, N.A.1
Rano, T.A.2
Peterson, E.P.3
Rasper, D.M.4
Timkey, T.5
-
14
-
-
0031746834
-
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria
-
Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, et al. (1998) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 64: 2240-2246.
-
(1998)
Appl Environ Microbiol
, vol.64
, pp. 2240-2246
-
-
Andersen, J.B.1
Sternberg, C.2
Poulsen, L.K.3
Bjorn, S.P.4
Givskov, M.5
-
15
-
-
0037119435
-
Genetic analysis of the twin arginine translocator secretion pathway in bacteria
-
DeLisa MP, Samuelson P, Palmer T, Georgiou G (2002) Genetic analysis of the twin arginine translocator secretion pathway in bacteria. J Biol Chem 277: 29825-29831.
-
(2002)
J Biol Chem
, vol.277
, pp. 29825-29831
-
-
DeLisa, M.P.1
Samuelson, P.2
Palmer, T.3
Georgiou, G.4
-
16
-
-
0034046020
-
The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue
-
Karzai AW, Roche ED, Sauer RT (2000) The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue. Nat Struct Biol 7: 449-455.
-
(2000)
Nat Struct Biol
, vol.7
, pp. 449-455
-
-
Karzai, A.W.1
Roche, E.D.2
Sauer, R.T.3
-
17
-
-
4344658879
-
SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags
-
Hersch GL, Baker TA, Sauer RT (2004) SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags. Proc Natl Acad Sci USA 101: 12136-12141.
-
(2004)
Proc Natl Acad Sci USA
, vol.101
, pp. 12136-12141
-
-
Hersch, G.L.1
Baker, T.A.2
Sauer, R.T.3
-
19
-
-
68349127373
-
HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification
-
Ohana RF, Encell LP, Zhao K, Simpson D, Slater MR, et al. (2009) HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification. Protein Expr Purif.
-
(2009)
Protein Expr Purif
-
-
Ohana, R.F.1
Encell, L.P.2
Zhao, K.3
Simpson, D.4
Slater, M.R.5
-
20
-
-
67651028031
-
Covalent immobilization of tobacco-etch-virus NIa protease: A useful tool for cleavage of the histidine tag of recombinant proteins
-
Puhl AC, Giacomini C, Irazoqui G, Batista-Viera F, Villarino A, et al. (2009) Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins. Biotechnol Appl Biochem 53: 165-174.
-
(2009)
Biotechnol Appl Biochem
, vol.53
, pp. 165-174
-
-
Puhl, A.C.1
Giacomini, C.2
Irazoqui, G.3
Batista-Viera, F.4
Villarino, A.5
-
21
-
-
77951138971
-
Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin
-
Steere AN, Roberts SE, Byrne SL, Dennis Chasteen N, Bobst CE, et al. Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin. Protein Expr Purif 72: 32-41.
-
Protein Expr Purif
, vol.72
, pp. 32-41
-
-
Steere, A.N.1
Roberts, S.E.2
Byrne, S.L.3
Dennis Chasteen, N.4
Bobst, C.E.5
-
22
-
-
33751217186
-
Monitoring regulated protein-protein interactions using split TEV
-
Wehr MC, Laage R, Bolz U, Fischer TM, Grunewald S, et al. (2006) Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3: 985-993.
-
(2006)
Nat Methods
, vol.3
, pp. 985-993
-
-
Wehr, M.C.1
Laage, R.2
Bolz, U.3
Fischer, T.M.4
Grunewald, S.5
-
23
-
-
66249103970
-
Efficient protein depletion by genetically controlled deprotection of a dormant N-degron
-
Taxis C, Stier G, Spadaccini R, Knop M (2009) Efficient protein depletion by genetically controlled deprotection of a dormant N-degron. Mol Syst Biol 5: 267.
-
(2009)
Mol Syst Biol
, vol.5
, pp. 267
-
-
Taxis, C.1
Stier, G.2
Spadaccini, R.3
Knop, M.4
-
24
-
-
77953570947
-
Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics
-
Boulware KT, Jabaiah A, Daugherty PS (2010) Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics. Biotechnol Bioeng 106: 339-346.
-
(2010)
Biotechnol Bioeng
, vol.106
, pp. 339-346
-
-
Boulware, K.T.1
Jabaiah, A.2
Daugherty, P.S.3
-
25
-
-
0024708229
-
Molecular genetic analysis of a plant virus polyprotein cleavage site: A model
-
Dougherty WG, Cary SM, Parks TD (1989) Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. Virology 171: 356-364.
-
(1989)
Virology
, vol.171
, pp. 356-364
-
-
Dougherty, W.G.1
Cary, S.M.2
Parks, T.D.3
-
26
-
-
0024730021
-
Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase
-
Dougherty WG, Parks TD, Cary SM, Bazan JF, Fletterick RJ (1989) Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase. Virology 172: 302-310.
-
(1989)
Virology
, vol.172
, pp. 302-310
-
-
Dougherty, W.G.1
Parks, T.D.2
Cary, S.M.3
Bazan, J.F.4
Fletterick, R.J.5
-
27
-
-
0037184981
-
Structural basis for the substrate specificity of tobacco etch virus protease
-
Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK, et al. (2002) Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem 277: 50564-50572.
-
(2002)
J Biol Chem
, vol.277
, pp. 50564-50572
-
-
Phan, J.1
Zdanov, A.2
Evdokimov, A.G.3
Tropea, J.E.4
Peters, H.K.5
-
28
-
-
18044397860
-
Mathematical expressions useful in the construction, description and evaluation of protein libraries
-
Bosley AD, Ostermeier M (2005) Mathematical expressions useful in the construction, description and evaluation of protein libraries. Biomol Eng 22: 57-61.
-
(2005)
Biomol Eng
, vol.22
, pp. 57-61
-
-
Bosley, A.D.1
Ostermeier, M.2
-
29
-
-
0028559783
-
Trinucleotide phosphoramidites: Ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis
-
Virnekas B, Ge L, Pluckthun A, Schneider KC, Wellnhofer G, et al. (1994) Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. Nucleic Acids Res 22: 5600-5607.
-
(1994)
Nucleic Acids Res
, vol.22
, pp. 5600-5607
-
-
Virnekas, B.1
Ge, L.2
Pluckthun, A.3
Schneider, K.C.4
Wellnhofer, G.5
-
30
-
-
0029810446
-
A convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries
-
Kayushin AL, Korosteleva MD, Miroshnikov AI, Kosch W, Zubov D, et al. (1996) A convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries. Nucleic Acids Res 24: 3748-3755.
-
(1996)
Nucleic Acids Res
, vol.24
, pp. 3748-3755
-
-
Kayushin, A.L.1
Korosteleva, M.D.2
Miroshnikov, A.I.3
Kosch, W.4
Zubov, D.5
-
31
-
-
0031259833
-
Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins
-
Nilsson J, Stahl S, Lundeberg J, Uhlen M, Nygren PA (1997) Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif 11: 1-16.
-
(1997)
Protein Expr Purif
, vol.11
, pp. 1-16
-
-
Nilsson, J.1
Stahl, S.2
Lundeberg, J.3
Uhlen, M.4
Nygren, P.A.5
-
32
-
-
33646797435
-
Mapping protease substrates by using a biotinylated phage substrate library
-
Scholle MD, Kriplani U, Pabon A, Sishtla K, Glucksman MJ, et al. (2006) Mapping protease substrates by using a biotinylated phage substrate library. ChemBioChem 7: 834-838.
-
(2006)
ChemBioChem
, vol.7
, pp. 834-838
-
-
Scholle, M.D.1
Kriplani, U.2
Pabon, A.3
Sishtla, K.4
Glucksman, M.J.5
-
33
-
-
46349100463
-
Engineering of protease variants exhibiting altered substrate specificity
-
Sellamuthu S, Shin BH, Lee ES, Rho SH, Hwang W, et al. (2008) Engineering of protease variants exhibiting altered substrate specificity. Biochem Biophys Res Commun 371: 122-126.
-
(2008)
Biochem Biophys Res Commun
, vol.371
, pp. 122-126
-
-
Sellamuthu, S.1
Shin, B.H.2
Lee, E.S.3
Rho, S.H.4
Hwang, W.5
-
34
-
-
0034460955
-
Sensitive genetic screen for protease activity based on a cyclic AMP signaling cascade in Escherichia coli
-
Dautin N, Karimova G, Ullmann A, Ladant D (2000) Sensitive genetic screen for protease activity based on a cyclic AMP signaling cascade in Escherichia coli. J Bacteriol 182: 7060-7066.
-
(2000)
J Bacteriol
, vol.182
, pp. 7060-7066
-
-
Dautin, N.1
Karimova, G.2
Ullmann, A.3
Ladant, D.4
-
35
-
-
33846872163
-
Methods for mapping protease specificity
-
Diamond S (2007) Methods for mapping protease specificity. Current Opinion in Chemical Biology 11: 46-51.
-
(2007)
Current Opinion in Chemical Biology
, vol.11
, pp. 46-51
-
-
Diamond, S.1
-
36
-
-
0020480638
-
pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts
-
Rüther U (1982) pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res 10: 5765-5772.
-
(1982)
Nucleic Acids Res
, vol.10
, pp. 5765-5772
-
-
Rüther, U.1
-
37
-
-
0035711194
-
Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency
-
Kapust RB, Tözsér J, Fox JD, Anderson DE, Cherry S, et al. (2001) Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng 14: 993-1000.
-
(2001)
Protein Eng
, vol.14
, pp. 993-1000
-
-
Kapust, R.B.1
Tözsér, J.2
Fox, J.D.3
Anderson, D.E.4
Cherry, S.5
-
38
-
-
0029000550
-
Expression and purification of a recombinant tobacco etch virus NIa proteinase: Biochemical analyses of the full-length and a naturally occurring truncated proteinase form
-
Parks TD, Howard ED, Wolpert TJ, Arp DJ, Dougherty WG (1995) Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form. Virology 210: 194-201.
-
(1995)
Virology
, vol.210
, pp. 194-201
-
-
Parks, T.D.1
Howard, E.D.2
Wolpert, T.J.3
Arp, D.J.4
Dougherty, W.G.5
-
39
-
-
0035996712
-
Processive degradation of nascent polypeptides, triggered by tandem AGA codons, limits the accumulation of recombinant tobacco etch virus protease in Escherichia coli BL21(DE3)
-
Kapust RB, Routzahn KM, Waugh DS (2002) Processive degradation of nascent polypeptides, triggered by tandem AGA codons, limits the accumulation of recombinant tobacco etch virus protease in Escherichia coli BL21(DE3). Protein Expr Purif 24: 61-70.
-
(2002)
Protein Expr Purif
, vol.24
, pp. 61-70
-
-
Kapust, R.B.1
Routzahn, K.M.2
Waugh, D.S.3
-
40
-
-
0027229928
-
Gene splicing by overlap extension
-
Horton RM, Ho SN, Pullen JK, Hunt HD, Cai Z, et al. (1993) Gene splicing by overlap extension. Meth Enzymol 217: 270-279.
-
(1993)
Meth Enzymol
, vol.217
, pp. 270-279
-
-
Horton, R.M.1
Ho, S.N.2
Pullen, J.K.3
Hunt, H.D.4
Cai, Z.5
-
41
-
-
0032787876
-
Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused
-
Kapust RB, Waugh DS (1999) Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci 8: 1668-1674.
-
(1999)
Protein Sci
, vol.8
, pp. 1668-1674
-
-
Kapust, R.B.1
Waugh, D.S.2
-
42
-
-
34250348569
-
A combined approach to improving large-scale production of tobacco etch virus protease
-
Blommel PG, Fox BG (2007) A combined approach to improving large-scale production of tobacco etch virus protease. Protein Expr Purif 55: 53-68.
-
(2007)
Protein Expr Purif
, vol.55
, pp. 53-68
-
-
Blommel, P.G.1
Fox, B.G.2
-
44
-
-
0029973636
-
FACS-optimized mutants of the green fluorescent protein (GFP)
-
Cormack BP, Valdivia RH, Falkow S (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
-
(1996)
Gene
, vol.173
, pp. 33-38
-
-
Cormack, B.P.1
Valdivia, R.H.2
Falkow, S.3
-
45
-
-
0029018327
-
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
-
Guzman LM, Belin D, Carson MJ, Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177: 4121-4130.
-
(1995)
J Bacteriol
, vol.177
, pp. 4121-4130
-
-
Guzman, L.M.1
Belin, D.2
Carson, M.J.3
Beckwith, J.4
-
46
-
-
0034725140
-
Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems
-
Bandmann N, Collet E, Leijen J, Uhlen M, Veide A, et al. (2000) Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems. J Biotechnol 79: 161-172.
-
(2000)
J Biotechnol
, vol.79
, pp. 161-172
-
-
Bandmann, N.1
Collet, E.2
Leijen, J.3
Uhlen, M.4
Veide, A.5
-
47
-
-
0343526348
-
Highthroughput protein expression of cDNA products as a tool in functional genomics
-
Larsson M, Graslund S, Yuan L, Brundell E, Uhlen M, et al. (2000) Highthroughput protein expression of cDNA products as a tool in functional genomics. J Biotechnol 80: 143-157.
-
(2000)
J Biotechnol
, vol.80
, pp. 143-157
-
-
Larsson, M.1
Graslund, S.2
Yuan, L.3
Brundell, E.4
Uhlen, M.5
-
48
-
-
0025675856
-
High efficiency transformation of Escherichia coli with plasmids
-
Inoue H, Nojima H, Okayama H (1990) High efficiency transformation of Escherichia coli with plasmids. Gene 96: 23-28.
-
(1990)
Gene
, vol.96
, pp. 23-28
-
-
Inoue, H.1
Nojima, H.2
Okayama, H.3
-
49
-
-
33645417226
-
High-throughput protein purification using an automated set-up for high-yield affinity chromatography
-
Steen J, Uhlén M, Hober S, Ottosson J (2006) High-throughput protein purification using an automated set-up for high-yield affinity chromatography. Protein Expr Purif 46: 173-178.
-
(2006)
Protein Expr Purif
, vol.46
, pp. 173-178
-
-
Steen, J.1
Uhlén, M.2
Hober, S.3
Ottosson, J.4
|