Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs

Biochimica et Biophysica Acta - Biomembranes

Volumn 1828, Issue 2, 2013, Pages 743-757

  Periasamy, Agalya ^a   Shadiac, Nadim ^a   Amalraj, Amritha ^a   Garajová, Soňa ^a,c   Nagarajan, Yagnesh ^a   Waters, Shane ^a   Mertens, Haydyn D T ^b   Hrmova, Maria ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

^b AUSTRALIAN SYNCHROTRON   (Australia)

^c INSTITUTE OF CHEMISTRY   (Slovakia)

Author keywords

(1,3) d Glucan; Family GT2 transferase; Small angle X ray scattering; Surfactant; Surfactant peptide; Topology

Indexed keywords

1,3 BETA GLUCAN SYNTHASE; 2 OLEOYL 1 PALMITOYLPHOSPHATIDYLCHOLINE; BLOOD CLOTTING FACTOR 10A; DIMYRISTOYLPHOSPHATIDYLCHOLINE; LIPOSOME; NANODISC; SCAFFOLD PROTEIN; SURFACTANT;

ARTICLE; CATALYSIS; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; ESCHERICHIA COLI; FLOTATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEMBRANE; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RHIZOBIUM; SYNCHROTRON RADIATION; WHEAT GERM; X RAY CRYSTALLOGRAPHY;

AGROBACTERIUM; BETA-GLUCANS; CATALYSIS; CELL-FREE SYSTEM; ESCHERICHIA COLI; GLUCOSE; GLUCOSYLTRANSFERASES; LIPOSOMES; MICROSCOPY, ELECTRON, TRANSMISSION; NANOPARTICLES; NANOTECHNOLOGY; PEPTIDES; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEINS; RNA, MESSENGER; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SURFACE-ACTIVE AGENTS; TRYPSIN; URIDINE DIPHOSPHATE;

AGROBACTERIUM; ESCHERICHIA COLI; TOBACCO ETCH VIRUS; TRITICUM AESTIVUM;

EID: 84870367042     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2012.10.003     Document Type: Article

References (53)

1. S.J. Stasinopoulos, P.R. Fisher, B.A. Stone, and V.A. Stanisich Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene Glycobiology 9 1999 31 41
2. M. Hrmova, B.A. Stone, and G.B. Fincher High-yield production, refolding and molecular modelling of the catalytic module of (1,3)-β-d-glucan (curdlan) synthase from Agrobacterium sp Glycoconj. J. 27 2010 461 476
3. B.A. Stone, A.K. Jacobs, M. Hrmova, R.A. Burton, and G.B. Fincher The biosynthesis of plant cell wall and related polysaccharides by enzymes of the GT2 and GT48 families P. Ulvskov, Plant Polysaccharides Series: Annual Plant Reviews 41 2011 Blackwell Publishing Danvers 109 166
4. B.L. Cantarel, P.M. Coutinho, C. Rancurel, T. Bernard, V. Lombard, and B. Henrissat The Carbohydrate-Active enZymes database (CAZy): an expert resource for glycogenomics Nucleic Acids Res. 37 2009 D233 D238
5. S.J. Charnock, and G.J. Davies Structure of the nucleotide-diphospho- sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms Biochemistry 18 1999 6380 6385
6. T. Osawa, N. Sugiura, H. Shimada, R. Hirooka, A. Tsuji, T. Shirakawa, K. Fukuyama, M. Kimura, K. Kimata, and Y. Kakuta Crystal structure of chondroitin polymerase from Escherichia coli K4 Biochem. Biophys. Res. Commun. 378 2009 10 14
7. A.L. Lovering, L.Y. Lin, E.W. Sewell, T. Spreter, E.D. Brown, and N.C. Strynadka Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis Nat. Struct. Mol. Biol. 17 2010 582 589
8. E.J. Stewart, F. Aslund, and J. Beckwith Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins EMBO J. 17 1998 5543 5550
9. Y. Tsunoda, N. Sakai, and K. Kikuchi Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli Protein Expr. Purif. 42 2005 268 277
10. A.S. Spirin, and J.R. Swartz Cell-free protein synthesis Methods and Protocols 2008 Wiley-VCH Verlag Weinheim
11. N. Farrokhi, M. Hrmova, R.A. Burton, and G.B. Fincher Heterologous and cell free expression systems D. Somers, P. Langridge, P. Gustafson, Plant genomics Meth. Mol. Biol. 513 2009 Humana Press Inc. Totowa 175 198
12. F. Katzen, G. Chang, and W. Kudlicki The past, present and future of cell-free protein synthesis Trends Biotechnol. 23 2005 150 156
13. S. Wagner, M.L. Bader, D. Drew, and J.-W. de Gier Rationalizing membrane protein overexpression Trends Biotechnol. 24 2006 364 371
14. L. Liguori, B. Marques, A. Villegas-Mendez, R. Rothe, and J.L. Lenormand Production of membrane proteins using cell-free expression systems Expert Rev. Proteomics 4 2007 79 90
15. D.F. Savage, C.L. Anderson, Y. Robles-Colmenares, Z.E. Newby, and R.M. Stroud Cell-free complements in vivo expression of the E. coli membrane proteome Protein Sci. 16 2007 966 976
16. D. Schwarz, V. Dötsch, and F. Bernhard Production of membrane proteins using cell-free expression systems Proteomics 8 2008 3933 3946
17. F. Junge, S. Haberstock, C. Roos, S. Stefer, D. Proverbio, V. Dötsch, and F. Bernhard Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins New Biotechnol. 28 2011 262 271
18. F. Junge, B. Schneider, S. Reckel, D. Schwarz, V. Dotsch, and F. Bernhard Large-scale production of functional membrane proteins Cell. Mol. Life Sci. 65 2008 1729 1755
19. A.S. Spirin, V.I. Baranov, L.A. Ryabova, S.Y. Ovodov, and Y.B. Alakhov A continuous cell-free translation system capable of producing polypeptides in high yield Science 242 1988 1162 1164
20. K. Madin, T. Sawasaki, T. Ogasawara, and Y. Endo A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes Proc. Natl. Acad. Sci. U.S.A. 97 2000 559 564
21. D.A. Vinarov, C.L.L. Newman, and J.L. Markley Wheat germ cell-free platform for eukaryotic protein production FEBS J. 273 2006 4160 4416
22. M. Aoki, T. Matsuda, Y. Tomo, Y. Miyata, M. Inoue, T. Kigawaa, and S. Yokoyama Automated system for high-throughput protein production using the dialysis cell-free method Protein Expr. Purif. 68 2009 128 136
23. T.H. Bayburt, Y.V. Grinkova, and S.G. Sligar Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins Nano Lett. 2 2002 853 856
24. I.G. Denisov, Y.V. Grinkova, A.A. Lazarides, and S.G. Sligar Directed self-assembly of monodisperse phospholipid bilayer nanodiscs with controlled size J. Am. Chem. Soc. 126 2004 3477 3487
25. H.D.T. Mertens, and D.I. Svergun Structural characterization of proteins and complexes using small-angle X-ray solution scattering J. Struct. Biol. 172 2010 128 141
26. T. Sawasaki, Y. Hasegawa, M. Tsuchimochi, N. Kamura, T. Ogasawara, T. Kuroita, and Y. Endo Bilayer cell-free protein synthesis system for high-throughput screening of gene products FEBS Lett. 514 2010 102 105
27. M.D. Abramoff, P.J. Magalhaes, and S.J. Ram Image processing with ImageJ Biophotonics Int. 11 2004 36 42
28. 6-tagged TEV protease Meth. Mol. Biol. 498 2009 297 307
29. F.J.D. Alvarez, C. Orelle, and A.L. Davidson Functional reconstitution of an ABC transporter in nanodiscs for use in electron paramagnetic resonance spectroscopy J. Am. Chem. Soc. 132 2010 9513 9515
30. M.V. Petoukhov, D. Franke, A.V. Shkumatov, G. Tria, A.G. Kikhney, M. Gajda, C. Gorba, H.D.T. Mertens, P.V. Konarev, and D.I. Svergun New developments in the ATSAS program package for small-angle scattering data analysis J. Appl. Crystallogr. 45 2012 342 350
31. D.I. Svergun Determination of the regularization parameter in indirect-transform methods using perceptual criteria J. Appl. Crystallogr. 25 1992 495 503
32. R.B. Kapust, J. Tözsér, J.D. Fox, D.E. Anderson, S. Cherry, T.D. Copeland, and D.S. Waugh Tobacco Etch Virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency Protein Eng. 14 2001 993 1000
33. E.M. Shematek, J.A. Braatz, E. Cabib, and E. Biosynthesis Biosynthesis of the yeast cell wall. I. Preparation and properties of (1,3)-β-glucan synthetase J. Biol. Chem. 255 1980 888 894
34. M. Hrmova, C.S. Taft, and C.P. Selitrennikoff (1,3)-β-d-Glucan synthase of Neurospora crassa: partial purification and characterization of solubilized enzyme activity Exp. Mycol. 13 1989 129 139
35. A. Rath, M. Glibowicka, V.G. Nadeau, G. Chen, and C.M. Deber Detergent binding explains anomalous SDS-PAGE migration of membrane proteins Proc. Natl. Acad. Sci. U.S.A. 106 2009 1760 1765
36. A. Nozawa, T. Ogasawara, S. Matsunaga, T. Iwasaki, T. Sawasaki, and Y. Endo Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system BMC Biotechnol. 11 2011 35 44
37. I.G. Denisov, M.A. McLean, A.W. Shaw, Y.V. Grinkova, and S.G. Sligar Thermotropic phase transition in soluble nanoscale lipid bilayers J. Phys. Chem. B 109 2004 15580 15588
38. B.J. Baas, I.G. Denisov, and S.G. Sligar Homotropic cooperativity of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment Arch. Biochem. Biophys. 430 2004 218 228
39. O. Glatter A new method for the evaluation of small-angle scattering data J. Appl. Crystallogr. 10 1977 415 421
40. N. Skar-Gislinge, J.B. Simonsen, K. Mortensen, R. Feidenhans, S.G. Sligar, B.L. Møller, T. Bjørnholm, and L. Arleth Elliptical structure of phospholipid bilayer nanodiscs encapsulated by scaffold proteins: casting the roles of the lipids and the protein J. Am. Chem. Soc. 132 2010 13713 13722
41. C. Pasquier, V.J. Promponas, G.A. Palaios, and J.S. Hamodrakas A novel method for predicting transmembrane segments in proteins based on a statistical analysis of the SwissProt database: the PRED-TMR algorithm Protein Eng. 12 1999 381 385
42. G.S. Waldo, B.M. Standish, J. Berendzen, and T.C. Terwilliger Rapid protein-folding assay using green fluorescent protein Nat. Biotechnol. 17 1999 691 695
43. D. Drew, S. Newstead, Y. Sonoda, H. Kim, G. von Heijne, and S. Iwata GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae Nat. Protoc. 3 2008 784 798
44. J.M. Hsieh, G.M. Besserer, M.G. Madej, H.-Q. Bui, S. Kwon, and J. Abramson Bridging the gap: a GFP based strategy for overexpression and purification of membrane proteins with intra and extracellular C termini Protein Sci. 19 2010 868 880
45. K. Mizutani, S. Yoshioka, Y. Mizutani, S. Iwata, and B. Mikami High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins Protein Expr. Purif. 77 2011 1 8
46. A. Deniaud, L. Liguori, I. Blesneac, J.L. Lenormand, and E. Pebay-Peyroula Crystallisation of the membrane protein hVDAC1 produced in cell-free system Biochim, Biophys. Acta - Biomembranes 1798 2010 1540 1546
47. L. Isaksson, J. Enberg, R. Neutze, B.G. Karlsson, and A. Pedersen Expression screening of membrane proteins with cell-free protein synthesis Protein Expr. Purif. 82 2012 218 225
48. P. Bazzacco, E. Billon-Deni, S. Sharma, L.J. Catoire, S. Mar, C. Le Bon, E. Point, J.-L. Banères, G. Durand, F. Zito, B. Pucci, and J.-L. Popot Nonionic homopolymeric amphipols: application to membrane protein folding, cell-free synthesis, and solution nuclear magnetic resonance Biochemistry 51 2012 1416 1430
49. N. Dowhan Molecular basis for membrane phospholipid diversity, why are there so many lipids? Annu. Rev. Biochem. 66 1997 199 232
50. X. Zhao, Y. Nagai, P.J. Reeves, P. Kiley, H.G. Khorana, and S. Zhang Designer peptide surfactants stabilize diverse functional membrane proteins Chem. Soc. Rev. 41 2012 1721 1728
51. R. Yan, X. Mo, A.M. Paredes, K. Dai, F. Lanza, M.A. Cruz, and R. Li Reconstitution of the platelet glycoprotein Ib-IX complex in phospholipid bilayer nanodiscs Biochemistry 50 2011 10598 10606
52. M.T. Marty, A. Das, and S.G. Sligar Ultra-thin layer MALDI mass spectrometry of membrane proteins in nanodiscs Anal. Bioanal. Chem. 402 2012 721 729
53. Y. Ihara, T. Takeda, F. Sakai, and Y. Hayashi Transferase activity of GhCesA2 (putative cotton cellulose 4-β-glucosyltransferase) expressed in Pichia pastoris J. Wood Sci. 48 2002 425 428