Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore

Science

Volumn 274, Issue 5294, 1996, Pages 1859-1866

  Song, Langzhou ^b   Hobaugh, Michael R ^b   Shustak, Christopher ^b   Cheley, Stephen ^b   Bayley, Hagan ^b   Gouaux, J Eric ^b  

^a UNIVERSITY OF CHICAGO   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

GLYCINE; HEMOLYSIN; PROTEIN SUBUNIT; STAPHYLOCOCCUS ALPHA TOXIN;

ARTICLE; GENETIC ENGINEERING; HYDROGEN BOND; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN QUATERNARY STRUCTURE; STAPHYLOCOCCUS AUREUS; TOXIN STRUCTURE;

AMINO ACID SEQUENCE; BACTERIAL TOXINS; CELL MEMBRANE; CRYSTALLOGRAPHY, X-RAY; HEMOLYSIN PROTEINS; HYDROGEN BONDING; LIPID BILAYERS; MEMBRANE POTENTIALS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; STAPHYLOCOCCUS AUREUS;

BASIDIOMYCOTA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 0030447720     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5294.1859     Document Type: Article

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66. We thank a balciurias for crystallization assistance, K. Cowtan P. Lox, B, Perman, D. Picot, B. Ramachandran, Z. Ben, X.-J. Yang, and members of tne Bayley, Souaux, Garavito, and Moffat groups for assistance and comments: and H.-M. Ke (U. of North Carolina) for area detector time and helpful suggestions Supported in part by funds from a PHS/NIH Shared instrumentation Grant and a Louis Block grant, respectively, for the FAST and R-Axis IIo area detectors. initial refinement was performed at the Pittsburgh Supercomputer Center. This work was supported in part by grants from the Office of Naval Research (J.E.G. and H.B.), the NIH (J.E.G.), the;Martin D and Virginia S, Kamen Sustaining Fund for junior faculty (J.E.G), the Searle Sohoiars Program (J.E.G.), the NSF (J.E.G.), and the DOE (H.B.), the Cancer Research Foundation Young investigator fund (J.E.G.), and the Medical Scientist Training Program at the University of Chicago (M.R.H.). The coordinates have been deposited at the Protein Data Bank at Brookhaven with accession number 7ahl.