A flow cytometry-based screening system for directed evolution of proteases

Journal of Biomolecular Screening

Volumn 16, Issue 3, 2011, Pages 285-294

  Tu, Ran ^a   Martinez, Ronny ^a   Prodanovic, Radivoje ^b   Klein, Mathias ^a   Schwaneberg, Ulrich ^a,b  

^a JACOBS UNIVERSITY BREMEN   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

directed evolution; flow cytometry; high throughput; in vitro compartmentalization; protease

Indexed keywords

ANTIPAIN; PROTEINASE; RHODAMINE 110; SUBTILISIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; CELL COMPARTMENTALIZATION; CELL ENCAPSULATION; CELL SELECTION; DRUG RESISTANCE; EMULSION; ENZYME ACTIVITY; FLOW CYTOMETRY; HIGH THROUGHPUT SCREENING; MUTATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

AMINO ACID SUBSTITUTION; BACILLUS SUBTILIS; DIRECTED MOLECULAR EVOLUTION; EMULSIONS; ENZYME ACTIVATION; ENZYME INHIBITORS; FLOW CYTOMETRY; GENE LIBRARY; HIGH-THROUGHPUT SCREENING ASSAYS; MODELS, MOLECULAR; MUTATION; OILS; PEPTIDE HYDROLASES; PROTEIN STRUCTURE, TERTIARY; SUBTILISINS;

BACILLUS SUBTILIS;

EID: 79954615462     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110396361     Document Type: Article

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