A Competitive Flow Cytometry Screening System for Directed Evolution of Therapeutic Enzyme

ACS Synthetic Biology

Volumn 4, Issue 7, 2015, Pages 768-775

  Cheng, Feng ^a   Kardashliev, Tsvetan ^a,b   Pitzler, Christian ^a   Shehzad, Aamir ^a,d   Lue, Hongqi ^c   Bernhagen, Jürgen ^c   Zhu, Leilei ^a   Schwaneberg, Ulrich ^a,b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b DWI LEIBNIZ INSTITUTE FOR INTERACTIVE MATERIALS   (Germany)

^c UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

^d KOHAT UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Pakistan)

Author keywords

arginine deiminase; competitive screening system; directed evolution; LiMEx; protein engineering; synthetic biology

Indexed keywords

ARGININE; ARGININE DEIMINASE; ENHANCED GREEN FLUORESCENT PROTEIN; ENZYME; LIGAND; GREEN FLUORESCENT PROTEIN; HYDROLASE;

ARTICLE; CANCER INHIBITION; CLONE; CONTROLLED STUDY; EVOLUTION; FLOW CYTOMETRY; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; MELANOMA CELL LINE; MOLECULAR LIBRARY; PHYSIOLOGICAL PROCESS; PRIORITY JOURNAL; PSEUDOMONAS; PSEUDOMONAS PLECOGLOSSICIDA; SCREENING; SUBSTRATE CONCENTRATION; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; HIGH THROUGHPUT SCREENING; MELANOMA; METABOLISM; MUTATION; PATHOLOGY; PROTEIN ENGINEERING; SKIN TUMOR; TUMOR CELL LINE;

PSEUDOMONAS PLECOGLOSSICIDA;

ARGININE; CELL LINE, TUMOR; DIRECTED MOLECULAR EVOLUTION; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYDROLASES; MELANOMA; MUTATION; PROTEIN ENGINEERING; PSEUDOMONAS; SKIN NEOPLASMS;

EID: 84937153707     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb500343g     Document Type: Article

References (25)

1. Zhu, L., Verma, R., Roccatano, D., Ni, Y., Sun, Z. H., and Schwaneberg, U. (2010) A potential antitumor drug (arginine deiminase) reengineered for efficient operation under physiological conditions ChemBioChem 11, 2294-2301
2. Ruff, A. J., Dennig, A., Wirtz, G., Blanusa, M., and Schwaneberg, U. (2012) Flow cytometer-based high-throughput screening system for accelerated directed evolution of P450 monooxygenases ACS Catal. 2, 2724-2728
3. Yang, G. Y. and Withers, S. G. (2009) Ultrahigh-throughput FACS-based screening for directed enzyme evolution ChemBioChem 10, 2704-2715
4. Dietrich, J. A., McKee, A. E., and Keasling, J. D. (2010) High-throughput metabolic engineering: Advances in small-molecule screening and selection Annu. Rev. Biochem. 79, 563-590
5. Binder, S., Schendzielorz, G., Stabler, N., Krumbach, K., Hoffmann, K., Bott, M., and Eggeling, L. (2012) A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level Genome Biol. 13, R40
6. Schendzielorz, G., Dippong, M., Grunberger, A., Kohlheyer, D., Yoshida, A., Binder, S., Nishiyama, C., Nishiyama, M., Bott, M., and Eggeling, L. (2014) Taking control over control: Use of product sensing in single cells to remove flux control at key enzymes in biosynthesis pathways ACS Synth. Biol. 3, 21-29
7. Siedler, S., Schendzielorz, G., Binder, S., Eggeling, L., Bringer, S., and Bott, M. (2014) SoxR as a single-cell biosensor for NADPH-consuming enzymes in Escherichia coli ACS Synth. Biol. 3, 41-47
8. Uchiyama, T., Abe, T., Ikemura, T., and Watanabe, K. (2005) Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes Nat. Biotechnol. 23, 88-93
9. Schallmey, M., Frunzke, J., Eggeling, L., and Marienhagen, J. (2014) Looking for the pick of the bunch: High-throughput screening of producing microorganisms with biosensors J. Curr. Opin. Biotechnol. 26C, 148-154
10. Zhu, L., Tee, K. L., Roccatano, D., Sonmez, B., Ni, Y., Sun, Z. H., and Schwaneberg, U. (2010) Directed evolution of an antitumor drug (arginine deiminase PpADI) for increased activity at physiological pH ChemBioChem 11, 691-697
11. Caldara, M., Dupont, G., Leroy, F., Goldbeter, A., De Vuyst, L., and Cunin, R. (2008) Arginine biosynthesis in Escherichia coli-Experimental perturbation and mathematical modeling J. Biol. Chem. 283, 6347-6358
12. Van Duyne, G. D., Ghosh, G., Maas, W. K., and Sigler, P. B. (1996) Structure of the oligomerization and L-arginine binding domain of the arginine repressor of Escherichia coli J. Mol. Biol. 256, 377-391
13. Krin, E., Laurent-Winter, C., Bertin, P. N., Danchin, A., and Kolb, A. (2003) Transcription regulation coupling of the divergent argG and metY promoters in Escherichia coli K-12 J. Bacteriol. 185, 3139-3146
14. Zhu, L., Cheng, F., Piatkowski, V., and Schwaneberg, U. (2014) Protein Engineering of the Antitumor Enzyme PpADI for Improved Thermal Resistance ChemBioChem 15, 276-283
15. Cheng, F., Zhu, L., Lue, H. Q., Bernhagen, J., and Schwaneberg, U. (2015) Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas Appl. Microbiol. Biotechnol. 99, 1237-1247
16. Ensor, C. M., Holtsberg, F. W., Bomalaski, J. S., and Clark, M. A. (2002) Pegylated arginine deiminase (ADI-SS PEG(20,000) (mw)) inhibits human melanomas and hepatocellular carcinomas in vitro and in vivo Cancer Res. 62, 5443-5450
17. Cheng, P. N. M., Lam, T. L., Lam, W. M., Tsui, S. M., Cheng, A. W. M., Lo, W. H., and Leung, Y. C. (2007) Pegylated recombinant human arginase (rhArg-peg(5,000mw)) inhibits the in vitro and in vivo proliferation of human hepatocellular carcinoma through arginine depletion Cancer Res. 67, 309-317
18. Stone, E. M., Glazer, E. S., Chantranupong, L., Cherukuri, P., Breece, R. M., Tierney, D. L., Curley, S. A., Iverson, B. L., and Georgiou, G. (2010) Replacing Mn(2+) with Co(2+) in human arginase I enhances cytotoxicity toward L-arginine auxotrophic cancer cell lines ACS Chem. Biol. 19, 333-342
19. Zheng, P. P., Hop, W. C., Luider, T. M., Smitt, P. A. E. S., and Kros, J. M. (2007) Increased levels of circulating endothelial progenitor cells and circulating endothelial nitric oxide synthase in patients with gliomas Ann. Neurol. 62, 40-48
20. Broome, J. D. (1981) L-Asparaginase: discovery and development as a tumor-inhibitory agent Cancer Treat. Rep. 65, 111-114
21. Lim, D. B., Oppenheim, J. D., Eckhardt, T., and Maas, W. K. (1987) Nucleotide sequence of the argR gene of Escherichia coli K-12 and isolation of its product, the arginine repressor Proc. Natl. Acad. Sci. U. S. A. 84, 6697-6701
22. Tolia, N. H. and Joshua-Tor, L. (2006) Strategies for protein coexpression in Escherichia coli Nat. Methods 3, 55-64
23. Hanahan, D., Jessee, J., and Bloom, F. R. (1991) Plasmid transformation of Escherichia coli and other bacteria Methods Enzymol. 204, 63-113
24. Miyazaki, K. and Takenouchi, M. (2002) Creating random mutagenesis libraries using megaprimer PCR of whole plasmid Biotechniques 33, 1033-1034
25. Archibald, R. M. (1945) Colorimetric determination of urea J. Biol. Chem. 157, 507-518