Reprogramming the specificity of sortase enzymes

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 37, 2014, Pages 13343-13348

  Dorr, Brent M ^a   Ham, Hyun Ok ^b,c   An, Chihui ^a   Chaikof, Elliot L ^b,c   Liu, David R ^a  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c HARVARD UNIVERSITY   (United States)

Author keywords

Enzyme specificity; Protein evolution

Indexed keywords

AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; FETUIN A; MUTANT PROTEIN; SORTASE A;

ANTAGONISTS AND INHIBITORS; BIOCATALYSIS; CHEMICAL STRUCTURE; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; ENZYMOLOGY; HUMAN; METABOLISM; STAPHYLOCOCCUS AUREUS;

ALPHA-2-HS-GLYCOPROTEIN; AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; BIOCATALYSIS; CYSTEINE ENDOPEPTIDASES; DIRECTED MOLECULAR EVOLUTION; HUMANS; MODELS, MOLECULAR; MUTANT PROTEINS; STAPHYLOCOCCUS AUREUS; SUBSTRATE SPECIFICITY;

EID: 84907246904     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1411179111     Document Type: Article

References (36)

1. Leader B, Baca QJ, Golan DE (2008) Protein therapeutics: A summary and pharmacological classification. Nat Rev Drug Discov 7(1): 21-39.
2. Fierz B, Kilic S, Hieb AR, Luger K, Muir TW (2012) Stability of nucleosomes containing homogenously ubiquitylated H2A and H2B prepared using semisynthesis. J Am Chem Soc 134(48): 19548-19551.
3. Ito T, et al. (2010) Highly oriented recombinant glycosyltransferases: site-specific immobilization of unstable membrane proteins by using Staphylococcus aureus sortase A. Biochemistry 49(11): 2604-2614.
4. Witte MD, et al. (2012) Preparation of unnatural N-to-N and C-to-C protein fusions. Proc Natl Acad Sci USA 109(30): 11993-11998.
5. Proft T (2010) Sortase-mediated protein ligation: An emerging biotechnology tool for protein modification and immobilisation. Biotechnol Lett 32(1): 1-10.
6. Pritz S, et al. (2007) Synthesis of biologically active peptide nucleic acid-peptide conjugates by sortase-mediated ligation. J Org Chem 72(10): 3909-3912.
7. Antos JM, Miller GM, Grotenbreg GM, Ploegh HL (2008) Lipid modification of proteins through sortase-catalyzed transpeptidation. J Am Chem Soc 130(48): 16338-16343.
8. Chan L, et al. (2007) Covalent attachment of proteins to solid supports and surfaces via Sortase-mediated ligation. PLoS ONE 2(11): e1164.
9. Chen I, Dorr BM, Liu DR (2011) A general strategy for the evolution of bond-forming enzymes using yeast display. Proc Natl Acad Sci USA 108(28): 11399-11404.
10. Kruger RG, et al. (2004) Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA. Biochemistry 43(6): 1541-1551.
11. Piotukh K, et al. (2011) Directed evolution of sortase A mutants with altered substrate selectivity profiles. J Am Chem Soc 133(44): 17536-17539.
12. Antos JM, et al. (2009) Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity. J Am Chem Soc 131(31): 10800-10801.
13. Race PR, et al. (2009) Crystal structure of Streptococcus pyogenes sortase A: Implications for sortase mechanism. J Biol Chem 284(11): 6924-6933.
14. Zong Y, Bice TW, Ton-That H, Schneewind O, Narayana SVL (2004) Crystal structures of Staphylococcus aureus sortase A and its substrate complex. J Biol Chem 279(30): 31383-31389.
15. Ilangovan U, Ton-That H, Iwahara J, Schneewind O, Clubb RT (2001) Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus. Proc Natl Acad Sci USA 98(11): 6056-6061.
16. Suree N, et al. (2009) The structure of the Staphylococcus aureus sortase-substrate complex reveals how the universally conserved LPXTG sorting signal is recognized. J Biol Chem 284(36): 24465-24477.
17. Kruger RG, Dostal P, McCafferty DG (2004) Development of a high-performance liquid chromatography assay and revision of kinetic parameters for the Staphylococcus aureus sortase transpeptidase SrtA. Anal Biochem 326(1): 42-48.
18. Tanaka T, Yamamoto T, Tsukiji S, Nagamune T (2008) Site-specific protein modification on living cells catalyzed by Sortase. ChemBioChem 9(5): 802-807.
19. Consortium TU; UniProt Consortium (2014) Activities at the Universal Protein Resource (UniProt). Nucleic Acids Res 42(Database issue, D1): D191-D198.
20. Nanjappa V, et al. (2014) Plasma Proteome Database as a resource for proteomics research: 2014 update. Nucleic Acids Res 42(Database issue, D1): D959-D965.
21. Jahnen-Dechent W, Heiss A, Schäfer C, Ketteler M (2011) Fetuin-A regulation of calcified matrix metabolism. Circ Res 108(12): 1494-1509.
22. Ketteler M, et al. (2003) Association of low fetuin-A (AHSG) concentrations in serum with cardiovascular mortality in patients on dialysis: A cross-sectional study. Lancet 361(9360): 827-833.
23. Jethwaney D, et al. (2005) Fetuin-A, a hepatocyte-specific protein that binds Plasmodium berghei thrombospondin-related adhesive protein: A potential role in infectivity. Infect Immun 73(9): 5883-5891.
24. Popp MW, Dougan SK, Chuang T-Y, Spooner E, Ploegh HL (2011) Sortase-catalyzed transformations that improve the properties of cytokines. Proc Natl Acad Sci USA 108(8): 3169-3174.
25. Xia X, Babcock JP, Blaber SI, Harper KM, Blaber M (2012) Pharmacokinetic properties of 2nd-generation fibroblast growth factor-1 mutants for therapeutic application. PLoS ONE 7(11): e48210.
26. Suh JM, et al. (2014) Endocrinization of FGF1 produces a neomorphic and potent insulin sensitizer. Nature.
27. Yanagisawa-Miwa A, et al. (1992) Salvage of infarcted myocardium by angiogenic action of basic fibroblast growth factor. Science 257(5075): 1401-1403.
28. Laham RJ, et al. (2005) Transendocardial and transepicardial intramyocardial fibroblast growth factor-2 administration: Myocardial and tissue distribution. Drug Metab Dispos 33(8): 1101-1107.
29. Butt TR, Edavettal SC, Hall JP, Mattern MR (2005) SUMO fusion technology for difficult-to-express proteins. Protein Expr Purif 43(1): 1-9.
30. Jordan SW, Chaikof EL (2007) Novel thromboresistant materials. J Vasc Surg 45(6, Suppl A): A104-A115.
31. Banerjee I, Pangule RC, Kane RS (2011) Antifouling coatings: Recent developments in the design of surfaces that prevent fouling by proteins, bacteria, and marine organisms. Adv Mater 23(6): 690-718.
32. Varadarajan N, Rodriguez S, Hwang B-Y, Georgiou G, Iverson BL (2008) Highly active and selective endopeptidases with programmed substrate specificities. Nat Chem Biol 4(5): 290-294.
33. Yi L, et al. (2013) Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries. Proc Natl Acad Sci USA 110(18): 7229-7234.
34. Nie Z (1992) Fetuin: Its enigmatic property of growth promotion. Am J Physiol 263(3 Pt 1): C551-C562.
35. Pal D, et al. (2012) Fetuin-A acts as an endogenous ligand of TLR4 to promote lipidinduced insulin resistance. Nat Med 18(8): 1279-1285.
36. Walsh G (2006) Biopharmaceutical benchmarks 2006. Nat Biotechnol 24(7): 769-776.