Novel fluorescence-assisted whole-cell assay for engineering and characterization of proteases and their substrates

Applied and Environmental Microbiology

Volumn 76, Issue 22, 2010, Pages 7500-7508

  Kostallas, George ^a   Samuelson, Patrik ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CELL ASSAYS; CELL SORTING; COEXPRESSED; DIFFERENT SUBSTRATES; E. COLI; FLUORESCENCE INTENSITIES; HIGH-THROUGHPUT; IMPROVED SOLUBILITY; IN-VIVO; PROTEASE ACTIVITIES; PROTEASE INHIBITOR; QUANTITATIVE ANALYSIS; SUBSTRATE PEPTIDES; SUBSTRATE SPECIFICITY; TEV PROTEASE; TOBACCO ETCH VIRUS PROTEASE;

CYTOLOGY; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; PEPTIDES; SOLUBILITY; VIRUSES;

SUBSTRATES;

PEPTIDE HYDROLASE; RECOMBINANT PROTEIN; VIRUS PROTEIN;

COLIFORM BACTERIUM; CYTOPLASM; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE; GENETIC ENGINEERING; PEPTIDE; SUBSTRATE; VIRUS;

ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; FLUORESCENCE; GENETICS; METABOLISM; METHODOLOGY; POTYVIRUS; PROTEIN ENGINEERING; STAINING;

ESCHERICHIA COLI; FLUORESCENCE; PEPTIDE HYDROLASES; POTYVIRUS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; STAINING AND LABELING; VIRAL PROTEINS;

ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

EID: 78649692108     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01558-10     Document Type: Article

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