High-throughput screening approaches and combinatorial development of biomaterials using microfluidics

Acta Biomaterialia

Volumn 34, Issue , 2016, Pages 1-20

  Barata, David ^a   Van Blitterswijk, Clemens ^a   Habibovic, Pamela ^a  

^a UNIVERSITY OF TWENTE   (Netherlands)

Author keywords

Biomaterials; Biomedical research; High throughput screening; Microfluidics; Organ on chip

Indexed keywords

CLINICAL RESEARCH; FLUIDIC DEVICES; MOLECULAR BIOLOGY; THROUGHPUT; TISSUE CULTURE;

BIOMEDICAL RESEARCH; CO-CULTURES; COMBINATORIAL SCREENINGS; CONTROLLED PROPERTIES; HIGH THROUGHPUT SCREENING; MICROFLUIDICS DEVICES; MICROFLUIDICS SYSTEMS; ON CHIPS; ORGAN-ON-CHIP; SCREENING APPROACHES;

MICROFLUIDICS;

BIOMATERIAL;

BIOMIMETICS; BIOPRINTING; CELL COMPARTMENTALIZATION; CELL SELECTION; HIGH THROUGHPUT SCREENING; HUMAN; HYDROGEL; MEDICAL RESEARCH; MICROENVIRONMENT; MICROFLUIDICS; NONHUMAN; PRIORITY JOURNAL; REVIEW; SCREENING; SURFACE PROPERTY; TUMOR SPHEROID; ANIMAL; COMBINATORIAL CHEMISTRY; PROCEDURES; SYNTHESIS;

ANIMALS; BIOCOMPATIBLE MATERIALS; BIOMIMETICS; COMBINATORIAL CHEMISTRY TECHNIQUES; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROFLUIDICS;

EID: 84941687449     PISSN: 17427061     EISSN: 18787568     Source Type: Journal    
DOI: 10.1016/j.actbio.2015.09.009     Document Type: Review

References (210)

1. S. Kobel, and M.P. Lutolf Biomaterials meet microfluidics: building the next generation of artificial niches Curr. Opin. Biotechnol. 22 2011 690 697
2. C.J. Bettinger, and J.T. Borenstein Biomaterials-based microfluidics for engineered tissue constructs Soft Matter 6 2010 4999 5015
3. P. Habibovic, T. Woodfield, K. de Groot, and C. van Blitterswijk Predictive value of in vitro and in vivo assays in bone and cartilage repair-What do they really tell us about the clinical performance? Adv. Exp. Med. Biol. 585 2006 327 360
4. G.M. Whitesides The origins and the future of microfluidics Nature 442 2006 368 373
5. S.F. Mao, D. Gao, W. Liu, H.B. Wei, and J.M. Lin Imitation of drug metabolism in human liver and cytotoxicity assay using a microfluidic device coupled to mass spectrometric detection Lab Chip 12 2012 219 226
6. P. Domachuk, K. Tsioris, F.G. Omenetto, and D.L. Kaplan Bio-microfluidics: biomaterials and biomimetic designs Adv. Mater. 22 2010 249 260
7. E. Berthier, E.W. Young, and D. Beebe Engineers are from PDMS-land, biologists are from polystyrenia Lab Chip 12 2012 1224 1237
8. A. van Reenen, A.M. de Jong, J.M. den Toonder, and M.W. Prins Integrated lab-on-chip biosensing systems based on magnetic particle actuation-a comprehensive review Lab Chip 14 2014 1966 1986
9. S. Choi, M. Goryll, L.Y.M. Sin, P.K. Wong, and J. Chae Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins Microfluid. Nanofluid. 10 2011 231 247
10. S. Kumar, S. Kumar, M.A. Ali, P. Anand, V.V. Agrawal, R. John, and et al. Microfluidic-integrated biosensors: prospects for point-of-care diagnostics Biotechnol. J. 8 2013 1267 1279
11. M. Zhan, L. Chingozha, and H. Lu Enabling systems biology approaches through microfabricated systems Anal. Chem. 85 2013 8882 8894
12. J.-H. Lee, Y. Gu, H. Wang, and W.Y. Lee Microfluidic 3D bone tissue model for high-throughput evaluation of wound-healing and infection-preventing biomaterials Biomaterials 33 2012 999 1006
13. Y. Zheng, J. Chen, M. Craven, N.W. Choi, S. Totorica, A. Diaz-Santana, and et al. In vitro microvessels for the study of angiogenesis and thrombosis Proc. Natl. Acad. Sci. U.S.A. 109 2012 9342 9347
14. A.K. Au, W. Lee, and A. Folch Mail-order microfluidics: evaluation of stereolithography for the production of microfluidic devices Lab Chip 14 2014 1294 1301
15. O.H. Paydar, C.N. Paredes, Y. Hwang, J. Paz, N.B. Shah, and R.N. Candler Characterization of 3D-printed microfluidic chip interconnects with integrated O-rings Sens. Actuators, A 205 2014 199 203
16. A.K. Au, N. Bhattacharjee, L.F. Horowitz, T.C. Chang, and A. Folch 3D-printed microfluidic automation Lab Chip 15 2015 1934 1941
17. M. Zhang, J. Wu, L. Wang, K. Xiao, and W. Wen A simple method for fabricating multi-layer PDMS structures for 3D microfluidic chips Lab Chip 10 2010 1199 1203
18. D.M. Titmarsh, J.E. Hudson, A. Hidalgo, A.G. Elefanty, E.G. Stanley, E.J. Wolvetang, and et al. Microbioreactor arrays for full factorial screening of exogenous and paracrine factors in human embryonic stem cell differentiation PLoS One 2012 7
19. B.-H. Chueh, D. Huh, C.R. Kyrtsos, T. Houssin, N. Futai, and S. Takayama Leakage-free bonding of porous membranes into layered microfluidic array systems Anal. Chem. 79 2007 3504 3508
20. B.R. Flachsbart, K. Wong, J.M. Iannacone, E.N. Abante, R.L. Vlach, P.A. Rauchfuss, and et al. Design and fabrication of a multilayered polymer microfluidic chip with nanofluidic interconnects via adhesive contact printing Lab Chip 6 2006 667 674
21. M. Gong, B.R. Flachsbart, M.A. Shannon, P.W. Bohn, and J.V. Sweedler Fluidic communication between multiple vertically segregated microfluidic channels connected by nanocapillary array membranes Electrophoresis 29 2008 1237 1244
22. X. Chen, L. Zhang, H. Li, J. Sun, H. Cai, and D. Cui Development of a multilayer microfluidic device integrated with a PDMS-cellulose composite film for sample pre-treatment and immunoassay Sens. Actuators, A 193 2013 54 58
23. S. Haeberle, and R. Zengerle Microfluidic platforms for lab-on-a-chip applications Lab Chip 7 2007 1094 1110
24. O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, and et al. Centrifugal microfluidic platforms: advanced unit operations and applications Chem. Soc. Rev. 2015
25. G. Velve-Casquillas, M. Le Berre, M. Piel, and P.T. Tran Microfluidic tools for cell biological research Nano Today 5 2010 28 47
26. S. Le Gac, and A. van den Berg Single cells as experimentation units in lab-on-a-chip devices Trends Biotechnol. 28 2010 55 62
27. A.R. Wheeler, W.R. Throndset, R.J. Whelan, A.M. Leach, R.N. Zare, Y.H. Liao, and et al. Microfluidic device for single-cell analysis Anal. Chem. 75 2003 3581 3586
28. D.D. Carlo, L.Y. Wu, and L.P. Lee Dynamic single cell culture array Lab Chip 6 2006 1445 1449
29. S.L. Faley, M. Copland, J. Reboud, and J.M. Cooper Cell chip array for microfluidic proteomics enabling rapid in situ assessment of intracellular protein phosphorylation Biomicrofluidics 2011 5
30. J. Akagi, J. Skommer, A. Matuszek, K. Takeda, Y. Fujimura, K. Khoshmanesh, and et al. Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry Proc. SPIE 2013 8615 10.1117/12.2001474
31. Y.-Y. Chiang, J. Stewart, C. Gizewski, P. Ehrhard, D. Janasek, J. West, Recording signal transduction dynamics with unprecedented temporal resolution, in: The Sixteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012), Japan, 2012.
32. M. Junkin, and S. Tay Microfluidic single-cell analysis for systems immunology Lab Chip 14 2014 1246 1260
33. D. Wlodkowic, S. Faley, M. Zagnoni, J.P. Wikswo, and J.M. Cooper Microfluidic single-cell array cytometry for the analysis of tumor apoptosis Anal. Chem. 81 2009 5517 5523
34. M. Chabert, and J.L. Viovy Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells Proc. Natl. Acad. Sci. U.S.A. 105 2008 3191 3196
35. D.J. Eastburn, A. Sciambi, and A.R. Abate Ultrahigh-throughput mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops Anal. Chem. 85 2013 8016 8021
36. E. Brouzes, M. Medkova, N. Savenelli, D. Marran, M. Twardowski, J.B. Hutchison, and et al. Droplet microfluidic technology for single-cell high-throughput screening Proc. Natl. Acad. Sci. U.S.A. 106 2009 14195 14200
37. I.K. Dimov, G. Kijanka, Y. Park, J. Ducree, T. Kang, and L.P. Lee Integrated microfluidic array plate (iMAP) for cellular and molecular analysis Lab Chip 11 2011 2701 2710
38. K. Yang, S. Han, Y. Shin, E. Ko, J. Kim, K.I. Park, and et al. A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment Biomaterials 34 2013 6607 6614
39. H.Y. Wang, N. Bao, and C. Lu A microfluidic cell array with individually addressable culture chambers Biosens. Bioelectron. 24 2008 613 617
40. M. Nourmohammadzadeh, J.F. Lo, M. Bochenek, J.E. Mendoza-Elias, Q. Wang, Z. Li, and et al. Microfluidic array with integrated oxygenation control for real-time live-cell imaging: effect of hypoxia on physiology of microencapsulated pancreatic islets Anal. Chem. 85 2013 11240 11249
41. E. Kang, Y.Y. Choi, Y. Jun, B.G. Chung, and S.H. Lee Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval Lab Chip 10 2010 2651 2654
42. J.L. Wilson, S. Suri, A. Singh, C.A. Rivet, H. Lu, and T.C. McDevitt Single-cell analysis of embryoid body heterogeneity using microfluidic trapping array Biomed. Microdevices 16 2014 79 90
43. K. Chung, Y. Kim, J.S. Kanodia, E. Gong, S.Y. Shvartsman, and H. Lu A microfluidic array for large-scale ordering and orientation of embryos Nat. Methods 8 2011 171-U03
44. E. Bier Drosophila, the golden bug, emerges as a tool for human genetics Nat. Rev. Genet. 6 2005 9 23
45. M.C. Leung, P.L. Williams, A. Benedetto, C. Au, K.J. Helmcke, M. Aschner, and et al. Caenorhabditis elegans: an emerging model in biomedical and environmental toxicology Toxicol. Sci. 106 2008 5 28
46. D. St Johnston The art and design of genetic screens: Drosophila melanogaster Nat. Rev. Genet. 3 2002 176 188
47. T.J. Levario, M. Zhan, B. Lim, S.Y. Shvartsman, and H. Lu Microfluidic trap array for massively parallel imaging of Drosophila embryos Nat. Protoc. 8 2013 721 736
48. J.A. Carr, A. Parashar, R. Gibson, A.P. Robertson, R.J. Martin, and S. Pandey A microfluidic platform for high-sensitivity, real-time drug screening on C. elegans and parasitic nematodes Lab Chip 11 2011 2385 2396
49. S.E. Hulme, S.S. Shevkoplyas, A.P. McGuigan, J. Apfeld, W. Fontana, and G.M. Whitesides Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans Lab Chip 10 2010 589 597
50. S.R. Lockery, S.E. Hulme, W.M. Roberts, K.J. Robinson, A. Laromaine, T.H. Lindsay, and et al. A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans Lab Chip 12 2012 2211 2220
51. E.M. Wielhouwer, S. Ali, A. Al-Afandi, M.T. Blom, M.B. Olde Riekerink, C. Poelma, and et al. Zebrafish embryo development in a microfluidic flow-through system Lab Chip 11 2011 1815 1824
52. J.P. Dexter, M.B. Tamme, C.H. Lind, and E.-M.S. Collins On-chip immobilization of planarians for in vivo imaging Sci. Rep. 2014 4
53. S. Kim, H.J. Kim, and N.L. Jeon Biological applications of microfluidic gradient devices Integr. Biol.-UK 2 2010 584 603
54. E. Berthier, and D.J. Beebe Gradient generation platforms: new directions for an established microfluidic technology Lab Chip 14 2014 3241 3247
55. E. Weibull, S. Matsui, M. Sakai, H. Andersson Svahn, and T. Ohashi Microfluidic device for generating a stepwise concentration gradient on a microwell slide for cell analysis Biomicrofluidics 7 2013 064115
56. H. Kim, J. Ali, K. Phuyal, S. Park, and M. Kim Investigation of bacterial chemotaxis using a simple three-point microfluidic system BioChip J. 9 2015 50 58
57. J.D. Wu, X. Wu, and F. Lin Recent developments in microfluidics-based chemotaxis studies Lab Chip 13 2013 2484 2499
58. G.M. Walker, J. Sai, A. Richmond, M. Stremler, C.Y. Chung, and J.P. Wikswo Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generator Lab Chip 5 2005 611 618
59. H. Somaweera, A. Ibragimov, and D. Pappas Generation of a chemical gradient across an array of 256 cell cultures in a single chip Analyst 138 2013 5566 5571
60. M.J. Kim, K.H. Lim, H.J. Yoo, S.W. Rhee, and T.H. Yoon Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (microFIC) Lab Chip 10 2010 415 417
61. B. Harink, S. Le Gac, D. Barata, C. van Blitterswijk, and P. Habibovic Microfluidic platform with four orthogonal and overlapping gradients for soluble compound screening in regenerative medicine research Electrophoresis 36 2015 475 484
62. B.G. Chung, L.A. Flanagan, S.W. Rhee, P.H. Schwartz, A.P. Lee, E.S. Monuki, and et al. Human neural stem cell growth and differentiation in a gradient-generating microfluidic device Lab Chip 5 2005 401 406
63. Y.Q. Zhang, W.J. Zhang, and L.D. Qin Mesenchymal-mode migration assay and antimetastatic drug screening with high-throughput microfluidic channel networks Angew. Chem. Int. Ed. 53 2014 2344 2348
64. N. Gopalakrishnan, R. Hannam, G.P. Casoni, D. Barriet, J.M. Ribe, M. Haug, and et al. Infection and immunity on a chip: a compartmentalised microfluidic platform to monitor immune cell behaviour in real time Lab Chip 15 2015 1481 1487
65. Y. Awwad, T. Geng, A.S. Baldwin, and C. Lu Single cell NF-κB dynamics under stimulant concentration gradient Anal. Chem. 84 2012 1224 1228
66. T.F. Byrd Iv, L.T. Hoang, E.G. Kim, M.E. Pfister, E.M. Werner, S.E. Arndt, and et al. The microfluidic multitrap nanophysiometer for hematologic cancer cell characterization reveals temporal sensitivity of the calcein-AM efflux assay Sci. Rep.-UK 4 2014 5117
67. S. Paliwal, P.A. Iglesias, K. Campbell, Z. Hilioti, A. Groisman, and A. Levchenko MAPK-mediated bimodal gene expression and adaptive gradient sensing in yeast Nature 446 2007 46 51
68. H.J. Yoo, J. Park, and T.H. Yoon High throughput cell cycle analysis using microfluidic image cytometry (μFIC) Cytometry A 83A 2013 356 362
69. E. Jastrzebska, S. Flis, A. Rakowska, M. Chudy, Z. Jastrzebski, A. Dybko, and et al. A microfluidic system to study the cytotoxic effect of drugs: the combined effect of celecoxib and 5-fluorouracil on normal and cancer cells Microchim. Acta 180 2013 895 901
70. R.O. Hynes The extracellular matrix: not just pretty fibrils Science 326 2009 1216 1219
71. J. Wu, Z. Mao, H. Tan, L. Han, T. Ren, and C. Gao Gradient biomaterials and their influences on cell migration Interface Focus 2 2012 337 355
72. J. Almodovar, T. Crouzier, S. Selimovic, T. Boudou, A. Khademhosseini, and C. Picart Gradients of physical and biochemical cues on polyelectrolyte multilayer films generated via microfluidics Lab Chip 13 2013 1562 1570
73. T.F. Didar, and M. Tabrizian Generating multiplex gradients of biomolecules for controlling cellular adhesion in parallel microfluidic channels Lab Chip 12 2012 4363 4371
74. L.S.M. Teixeira, J. Patterson, and F.P. Luyten Skeletal tissue regeneration: where can hydrogels play a role? Int. Orthop. 38 2014 1861 1876
75. S. Cosson, S. Allazetta, and M.P. Lutolf Patterning of cell-instructive hydrogels by hydrodynamic flow focusing Lab Chip 13 2013 2099 2105
76. C. Neils, Z. Tyree, B. Finlayson, and A. Folch Combinatorial mixing of microfluidic streams Lab Chip 4 2004 342 350
77. P.J. Hung, P.J. Lee, P. Sabounchi, R. Lin, and L.P. Lee Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays Biotechnol. Bioeng. 89 2005 1 8
78. Y.H. Jang, M.J. Hancock, S.B. Kim, S. Selimovic, W.Y. Sim, H. Bae, and et al. An integrated microfluidic device for two-dimensional combinatorial dilution Lab Chip 11 2011 3277 3286
79. R. Sinha, S. Le Gac, N. Verdonschot, A. van den Berg, B. Koopman, and J. Rouwkema A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells Lab Chip 15 2015 429 439
80. D. Kilinc, A. Blasiak, J.J. O'Mahony, and G.U. Lee Low piconewton towing of CNS axons against diffusing and surface-bound repellents requires the inhibition of motor protein-associated pathways Sci. Rep.-UK 2014 4
81. J. Li, L. Zhu, M. Zhang, and F. Lin Microfluidic device for studying cell migration in single or co-existing chemical gradients and electric fields Biomicrofluidics 2012 6
82. K. Hattori, S. Sugiura, and T. Kanamori Microenvironment array chip for cell culture environment screening Lab Chip 11 2011 212 214
83. Y. Yang, K. Kulangara, J. Sia, L. Wang, and K.W. Leong Engineering of a microfluidic cell culture platform embedded with nanoscale features Lab Chip 11 2011 1638 1646
84. L. Wang, W. Liu, Y. Wang, J.-c. Wang, Q. Tu, R. Liu, and et al. Construction of oxygen and chemical concentration gradients in a single microfluidic device for studying tumor cell-drug interactions in a dynamic hypoxia microenvironment Lab Chip 13 2013 695 705
85. S. Garcia, R. Sunyer, A. Olivares, J. Noailly, J. Atencia, and X. Trepat Generation of stable orthogonal gradients of chemical concentration and substrate stiffness in a microfluidic device Lab Chip 2015
86. J. Kim, D. Taylor, N. Agrawal, H. Wang, H. Kim, A. Han, and et al. A programmable microfluidic cell array for combinatorial drug screening Lab Chip 12 2012 1813 1822
87. G.Y. Huang, L.H. Zhou, Q.C. Zhang, Y.M. Chen, W. Sun, F. Xu, and et al. Microfluidic hydrogels for tissue engineering Biofabrication 3 2011 012001
88. J.D. Wan Microfluidic-based synthesis of hydrogel particles for cell microencapsulation and cell-based drug delivery Polymers-Basel 4 2012 1084 1108
89. B.M. Baker, B. Trappmann, S.C. Stapleton, E. Toro, and C.S. Chen Microfluidics embedded within extracellular matrix to define vascular architectures and pattern diffusive gradients Lab Chip 13 2013 3246 3252
90. Y. Shin, S. Han, J.S. Jeon, K. Yamamoto, I.K. Zervantonakis, R. Sudo, and et al. Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels Nat. Protoc. 7 2012 1247 1259
91. Y.C. Toh, T.C. Lim, D. Tai, G.F. Xiao, D. van Noort, and H.R. Yu A microfluidic 3D hepatocyte chip for drug toxicity testing Lab Chip 9 2009 2026 2035
92. G.M. Price, K.K. Chu, J.G. Truslow, M.D. Tang-Schomer, A.P. Golden, J. Mertz, and et al. Bonding of macromolecular hydrogels using perturbants J. Am. Chem. Soc. 130 2008 6664-+
93. S. Cosson, S.A. Kobel, and M.P. Lutolf Capturing complex protein gradients on biomimetic hydrogels for cell-based assays Adv. Funct. Mater. 19 2009 3411 3419
94. N.D. Dinh, Y.Y. Chiang, H. Hardelauf, J. Baumann, E. Jackson, S. Waide, and et al. Microfluidic construction of minimalistic neuronal co-cultures Lab Chip 13 2013 1402 1412
95. G.W. Si, W. Yang, S.Y. Bi, C.X. Luo, and Q. Ouyang A parallel diffusion-based microfluidic device for bacterial chemotaxis analysis Lab Chip 12 2012 1389 1394
96. B. Carrion, C.P. Huang, C.M. Ghajar, S. Kachgal, E. Kniazeva, N.L. Jeon, and et al. Recreating the perivascular niche ex vivo using a microfluidic approach Biotechnol. Bioeng. 107 2010 1020 1028
97. S. Ostrovidov, N. Annabi, A. Seidi, M. Ramalingam, F. Dehghani, H. Kaji, and et al. Controlled release of drugs from gradient hydrogels for high-throughput analysis of cell-drug interactions Anal. Chem. 84 2012 1302 1309
98. F. Piraino, G. Camci-Unal, M.J. Hancock, M. Rasponi, and A. Khademhosseini Multi-gradient hydrogels produced layer by layer with capillary flow and crosslinking in open microchannels Lab Chip 12 2012 659 661
99. J. Malda, J. Visser, F.P. Melchels, T. Jungst, W.E. Hennink, W.J.A. Dhert, and et al. 25th anniversary article: engineering hydrogels for biofabrication Adv. Mater. 25 2013 5011 5028
100. D. Gao, J.J. Liu, H.B. Wei, H.F. Li, G.S. Guo, and J.M. Lin A microfluidic approach for anticancer drug analysis based on hydrogel encapsulated tumor cells Anal. Chim. Acta 665 2010 7 14
101. N.S. Bhise, J. Ribas, V. Manoharan, Y.S. Zhang, A. Polini, S. Massa, and et al. Organ-on-a-chip platforms for studying drug delivery systems J. Control. Release 190 2014 82 93
102. S.N. Bhatia, and D.E. Ingber Microfluidic organs-on-chips Nat. Biotechnol. 32 2014 760 772
103. R.W. Muthard, and S.L. Diamond Side view thrombosis microfluidic device with controllable wall shear rate and transthrombus pressure gradient Lab Chip 13 2013 1883 1891
104. M. Li, N.A. Hotaling, D.N. Ku, and C.R. Forest Microfluidic thrombosis under multiple shear rates and antiplatelet therapy doses PLoS One 2014 9
105. S. Chung, R. Sudo, V. Vickerman, I. Zervantonakis, and R. Kamm Microfluidic platforms for studies of angiogenesis, cell migration, and cell-cell interactions Ann. Biomed. Eng. 38 2010 1164 1177
106. E.W.K. Young Advances in microfluidic cell culture systems for studying angiogenesis J. Lab. Autom. 18 2013 427 436
107. S.H. Lim, C. Kim, A.R. Aref, R.D. Kamm, and M. Raghunath Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting Integr. Biol.-UK 5 2013 1474 1484
108. K.H. Wong, J.M. Chan, R.D. Kamm, and J. Tien Microfluidic models of vascular functions Annu. Rev. Biomed. Eng. 14 2012 205 230
109. R. Booth, and H. Kim Characterization of a microfluidic in vitro model of the blood-brain barrier ([small mu]BBB) Lab Chip 12 2012 1784 1792
110. B. Prabhakarpandian, M.-C. Shen, J.B. Nichols, I.R. Mills, M. Sidoryk-Wegrzynowicz, M. Aschner, and et al. SyM-BBB: a microfluidic blood brain barrier model Lab Chip 13 2013 1093 1101
111. L.M. Griep, F. Wolbers, B. de Wagenaar, P.M. ter Braak, B.B. Weksler, I.A. Romero, and et al. BBB on chip: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function Biomed. Microdevices 15 2013 145 150
112. Y.H. Hsu, M.L. Moya, C.C.W. Hughes, S.C. George, and A.P. Lee A microfluidic platform for generating large-scale nearly identical human microphysiological vascularized tissue arrays Lab Chip 13 2013 2990 2998
113. A. Hasan, A. Paul, N.E. Vrana, X. Zhao, A. Memic, Y.S. Hwang, and et al. Microfluidic techniques for development of 3D vascularized tissue Biomaterials 35 2014 7308 7325
114. J. Friedrich, R. Ebner, and L.A. Kunz-Schughart Experimental anti-tumor therapy in 3-D: spheroids-old hat or new challenge? Int. J. Radiat. Biol. 83 2007 849 871
115. F. Hirschhaeuser, H. Menne, C. Dittfeld, J. West, W. Mueller-Klieser, and L.A. Kunz-Schughart Multicellular tumor spheroids: an underestimated tool is catching up again J. Biotechnol. 148 2010 3 15
116. D. Herrmann, J.R.W. Conway, C. Vennin, A. Magenau, W.E. Hughes, J.P. Morton, and et al. Three-dimensional cancer models mimic cell-matrix interactions in the tumour microenvironment Carcinogenesis 35 2014 1671 1679
117. X. Xu, M.C. Farach-Carson, and X. Jia Three-dimensional in vitro tumor models for cancer research and drug evaluation Biotechnol. Adv. 32 2014 1256 1268
118. K. Kwapiszewska, A. Michalczuk, M. Rybka, R. Kwapiszewski, and Z. Brzozka A microfluidic-based platform for tumour spheroid culture, monitoring and drug screening Lab Chip 14 2014 2096 2104
119. J. Ruppen, L. Cortes-Dericks, E. Marconi, G. Karoubi, R.A. Schmid, R. Peng, and et al. A microfluidic platform for chemoresistive testing of multicellular pleural cancer spheroids Lab Chip 14 2014 1198 1205
120. O. Frey, P.M. Misun, D.A. Fluri, J.G. Hengstler, and A. Hierlemann Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis Nat. Commun. 2014 5
121. D. Majumdar, Y. Gao, D. Li, and D.J. Webb Co-culture of neurons and glia in a novel microfluidic platform J. Neurosci. Methods 196 2011 38 44
122. H. Hardelauf, J. Sisnaiske, A.A. Taghipour-Anvari, P. Jacob, E. Drabiniok, U. Marggraf, and et al. High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes Lab Chip 11 2011 2763 2771
123. D. Huh, D.C. Leslie, B.D. Matthews, J.P. Fraser, S. Jurek, G.A. Hamilton, and et al. A human disease model of drug toxicity-induced pulmonary edema in a lung-on-a-chip microdevice Sci. Transl. Med. 2012 4
124. D. Huh, B.D. Matthews, A. Mammoto, M. Montoya-Zavala, H.Y. Hsin, and D.E. Ingber Reconstituting organ-level lung functions on a chip Science 328 2010 1662 1668
125. J.S. Jeon, S. Bersini, M. Gilardi, G. Dubini, J.L. Charest, M. Moretti, and et al. Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation Proc. Natl. Acad. Sci. U.S.A. 112 2015 214 219
126. A.G. Sciancalepore, F. Sallustio, S. Girardo, L.G. Passione, A. Camposeo, E. Mele, and et al. A bioartificial renal tubule device embedding human renal stem/progenitor cells PLoS One 2014 9
127. A. Polini, L. Prodanov, N.S. Bhise, V. Manoharan, M.R. Dokmeci, and A. Khademhosseini Organs-on-a-chip: a new tool for drug discovery Expert Opin. Drug Discovery 9 2014 335 352
128. D. Huh, G.A. Hamilton, and D.E. Ingber From 3D cell culture to organs-on-chips Trends Cell Biol. 21 2011 745 754
129. B. Harink, S. Le Gac, R. Truckenmuller, C. van Blitterswijk, and P. Habibovic Regeneration-on-a-chip? The perspectives on use of microfluidics in regenerative medicine Lab Chip 13 2013 3512 3528
130. E.C. Jensen, A.M. Stockton, T.N. Chiesl, J. Kim, A. Bera, and R.A. Mathies Digitally programmable microfluidic automaton for multiscale combinatorial mixing and sample processing Lab Chip 13 2013 288 296
131. N.T. Huang, S.C. Truxal, Y.C. Tung, A. Hsiao, S. Takayama, and K. Kurabayashi High-speed tuning of visible laser wavelength using a nanoimprinted grating optical tunable filter Appl. Phys. Lett. 95 2009
132. T.L. Chen, M. Ciocarlie, S. Cousins, P.M. Grice, K. Hawkins, K. Hsiao, and et al. Robots for humanity using assistive robotics to empower people with disabilities IEEE Robot. Autom. Mag. 20 2013 30 39
133. S.W. Hu, B.Y. Xu, W.K. Ye, X.H. Xia, H.Y. Chen, and J.J. Xu Versatile microfluidic droplets array for bioanalysis ACS Appl. Mater. Interfaces 7 2015 935 940
134. Q.Q. Zhang, S.J. Zeng, J.H. Qin, and B.C. Lin Microfluidic droplet trapping array as nanoliter reactors for gas-liquid chemical reaction Electrophoresis 30 2009 3181 3188
135. G.S. Du, J.Z. Pan, S.P. Zhao, Y. Zhu, J.M.J. den Toonder, and Q. Fang Cell-based drug combination screening with a microfluidic droplet array system Anal. Chem. 85 2013 6740 6747
136. S. Allazetta, T.C. Hausherr, and M.P. Lutolf Microfluidic synthesis of cell-type-specific artificial extracellular matrix hydrogels Biomacromolecules 14 2013 1122 1131
137. P. Agarwal, S. Zhao, P. Bielecki, W. Rao, J.K. Choi, Y. Zhao, and et al. One-step microfluidic generation of pre-hatching embryo-like core-shell microcapsules for miniaturized 3D culture of pluripotent stem cells Lab Chip 13 2013 4525 4533
138. V. Miralles, A. Huerre, H. Williams, B. Fournie, and M.C. Jullien A versatile technology for droplet-based microfluidics: thermomechanical actuation Lab Chip 2015
139. E.J. Griffith, and S. Akella Coordinating multiple droplets in planar array digital microfluidic systems Int. J. Robot. Res. 24 2005 933 949
140. D.Q. Jin, Y. Zhu, and Q. Fang Swan probe: a nanoliter-scale and high-throughput sampling interface for coupling electrospray ionization mass spectrometry with microfluidic droplet array and multiwell plate Anal. Chem. 86 2014 10796 10803
141. Y. Su, Y. Zhu, and Q. Fang A multifunctional microfluidic droplet-array chip for analysis by electrospray ionization mass spectrometry Lab Chip 13 2013 1876 1882
142. Y. Zhu, H. Chen, G.S. Du, and Q. Fang Microfluidic droplet-array liquid-liquid chromatography based on droplet trapping technique Lab Chip 12 2012 4350 4354
143. A.Y. Hsiao, Y.S. Torisawa, Y.C. Tung, S. Sud, R.S. Taichman, K.J. Pienta, and et al. Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids Biomaterials 30 2009 3020 3027
144. J. Thiele, M. Windbergs, A.R. Abate, M. Trebbin, H.C. Shum, S. Forster, and et al. Early development drug formulation on a chip: fabrication of nanoparticles using a microfluidic spray dryer Lab Chip 11 2011 2362 2368
145. S.H. Kim, J.W. Kim, D.H. Kim, S.H. Han, and D.A. Weitz Polymersomes containing a hydrogel network for high stability and controlled release Small 9 2013 124 131
146. P.M. Valencia, E.M. Pridgen, M. Rhee, R. Langer, O.C. Farokhzad, and R. Karnik Microfluidic platform for combinatorial synthesis and optimization of targeted nanoparticles for cancer therapy ACS Nano 7 2013 10671 10680
147. H. Zec, T.D. Rane, and T.H. Wang Microfluidic platform for on-demand generation of spatially indexed combinatorial droplets Lab Chip 12 2012 3055 3062
148. A.B. Theberge, E. Mayot, A. El Harrak, F. Kleinschmidt, W.T. Huck, and A.D. Griffiths Microfluidic platform for combinatorial synthesis in picolitre droplets Lab Chip 12 2012 1320 1326
149. C.H. Yang, K.S. Huang, P.W. Lin, and Y.C. Lin Using a cross-flow microfluidic chip and external crosslinking reaction for monodisperse TPP-chitosan microparticles Sens. Actuators, B-Chem. 124 2007 510 516
150. C.E.Y. Cha, J. Oh, K. Kim, Y.L. Qiu, M. Joh, S.R. Shin, and et al. Microfluidics-assisted fabrication of gelatin-silica core-shell microgels for injectable tissue constructs Biomacromolecules 15 2014 283 290
151. H. Andrade-Zaldívar, L. Santos, and A. De León Rodríguez Expansion of human hematopoietic stem cells for transplantation: trends and perspectives Cytotechnology 56 2008 151 160
152. S. Kedong, F. Xiubo, L. Tianqing, H. Macedo, J. LiLi, F. Meiyun, and et al. Simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived from umbilical cord blood J. Mater. Sci. - Mater. Med. 21 2010 3183 3193
153. M. Buitinga, R. Truckenmüller, M.A. Engelse, L. Moroni, H.W.M. Ten Hoopen, C.A. van Blitterswijk, and et al. Microwell scaffolds for the extrahepatic transplantation of islets of langerhans PLoS One 8 2013 e64772
154. T. Siminiak, and M. Kurpisz Myocardial replacement therapy Circulation 108 2003 1167 1171
155. A. Fernandes-Platzgummer, M.M. Diogo, R.P. Baptista, C.L. da Silva, and J.M. Cabral Scale-up of mouse embryonic stem cell expansion in stirred bioreactors Biotechnol. Prog. 27 2011 1421 1432
156. S.M. Azarin, and S.P. Palecek Development of scalable culture systems for human embryonic stem cells Biochem. Eng. J. 48 2010 378 384
157. J. Carmelo, A. Fernandes-Platzgummer, J.S. Cabral, and C. da Silva Scalable ex vivo expansion of human mesenchymal stem/stromal cells in microcarrier-based stirred culture systems K. Turksen, Stem Cells and Good Manufacturing Practices 2015 Springer New York 147 159
158. A.A. Tomei, V. Manzoli, C.A. Fraker, J. Giraldo, D. Velluto, M. Najjar, and et al. Device design and materials optimization of conformal coating for islets of Langerhans Proc. Natl. Acad. Sci. U.S.A. 111 2014 10514 10519
159. Y. Teramura, and H. Iwata Bioartificial pancreas microencapsulation and conformal coating of islet of Langerhans Adv. Drug Deliv. Rev. 62 2010 827 840
160. H.F. Chan, Y. Zhang, Y.P. Ho, Y.L. Chiu, Y. Jung, and K.W. Leong Rapid formation of multicellular spheroids in double-emulsion droplets with controllable microenvironment Sci. Rep.-UK 2013 3
161. K.A. Hyun, and H.I. Jung Advances and critical concerns with the microfluidic enrichments of circulating tumor cells Lab Chip 14 2014 45 56
162. C.A. Bichsel, S. Gobaa, S. Kobel, C. Secondini, G.N. Thalmann, M.G. Cecchini, and et al. Diagnostic microchip to assay 3D colony-growth potential of captured circulating tumor cells Lab Chip 12 2012 2313 2316
163. B.Y. Zhang, J.V. Green, S.K. Murthy, and M. Radisic Label-free enrichment of functional cardiomyocytes using microfluidic deterministic lateral flow displacement PLoS One 2012 7
164. A. Tripathi, J. Riddell, and N. Chronis A biochip with a 3D microfluidic architecture for trapping white blood cells Sens. Actuators, B Chem. 186 2013 244 251
165. H.W. Wu, X.Z. Lin, S.M. Hwang, and G.B. Lee A microfluidic device for separation of amniotic fluid mesenchymal stem cells utilizing louver-array structures Biomed. Microdevices 11 2009 1297 1307
166. C. Chun-Li, C.A. Savran, S. Jalal, and D.E. Matei Micro-aperture chip system for high-throughput immunomagnetic cell detection Sensors, IEEE 2012 1 4
167. R.M. Cooper, D.C. Leslie, K. Domansky, A. Jain, C. Yung, M. Cho, and et al. A microdevice for rapid optical detection of magnetically captured rare blood pathogens Lab Chip 14 2014 182 188
168. Q.S. Chen, J. Wu, Y.D. Zhang, Z. Lin, and J.M. Lin Targeted isolation and analysis of single tumor cells with aptamer-encoded microwell array on microfluidic device Lab Chip 12 2012 5180 5185
169. J.H. Chung, Y.J. Kim, and E. Yoon Highly-efficient single-cell capture in microfluidic array chips using differential hydrodynamic guiding structures Appl. Phys. Lett. 98 2011
170. C.X. Luo, H. Li, C.Y. Xiong, X.L. Peng, Q.L. Kou, Y. Chen, and et al. The combination of optical tweezers and microwell array for cells physical manipulation and localization in microfluidic device Biomed. Microdevices 9 2007 573 578
171. M. Werner, F. Merenda, J. Piguet, R.P. Salathe, and H. Vogel Microfluidic array cytometer based on refractive optical tweezers for parallel trapping, imaging and sorting of individual cells Lab Chip 11 2011 2432 2439
172. L.J. Millet, K. Park, N.N. Watkins, K.J. Hsia, R. Bashir, Separating beads and cells in multi-channel microfluidic devices using dielectrophoresis and laminar flow, J. Vis. Exp. (48)(2011), http://dx.doi.org/10.3791/2545.
173. A. Valero, T. Braschler, N. Demierre, and P. Renaud A miniaturized continuous dielectrophoretic cell sorter and its applications Biomicrofluidics 4 2010 022807
174. X. Chen, J.Q. Liu, J. Zhu, Y.F. Sun, and J. Fan Optimization of microfluidic immunomagnetic chip for circulating tumor cell capture Sens. Mater. 25 2013 667 671
175. P. Chen, Y.-Y. Huang, K. Hoshino, and J.X.J. Zhang Microscale magnetic field modulation for enhanced capture and distribution of rare circulating tumor cells Sci. Rep. 2015 5
176. S.N. Wang, X.L. Zhang, W.X. Wang, and L.J. Lee Semicontinuous flow electroporation chip for high-throughput transfection on mammalian cells Anal. Chem. 81 2009 4414 4421
177. N. Hu, X.L. Zhang, J. Yang, S.W. Joo, and S.Z. Qian A cell electrofusion microfluidic chip with micro-cavity microelectrode array Microfluid. Nanofluid. 15 2013 151 160
178. N. Hu, J. Yang, S.W. Joo, A.N. Banerjee, and S. Qian Cell electrofusion in microfluidic devices: a review Sens. Actuators, B: Chem. 178 2013 63 85
179. R.M. Schoeman, E.W.M. Kemna, F. Wolbers, and A. van den Berg High-throughput deterministic single-cell encapsulation and droplet pairing, fusion, and shrinkage in a single microfluidic device Electrophoresis 35 2014 385 392
180. C. Wyatt Shields Iv, C.D. Reyes, and G.P. Lopez Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation Lab Chip 15 2015 1230 1249
181. 4 nanocomposite: synthesis, characterization, antioxidant activity, and its application as a biosensor Int. J. Polym. Mater. Polym. 64 2015 175 183
182. Z. Bagheryan, J.B. Raoof', and R. Ojani Development of a new gquadruplex biosensor with the functionalized SBA-15-Au nanoparticles: a platform for selecting gquadruplex-binding ligands Sens. Actuators, B: Chem. 213 2015 124 130
183. P. Guo, E.W. Hall, R. Schirhagl, H. Mukaibo, C.R. Martin, and R.N. Zare Microfluidic capture and release of bacteria in a conical nanopore array Lab Chip 12 2012 558 561
184. U. Shimanovich, I. Efimov, T.O. Mason, P. Flagmeier, A.K. Buell, A. Gedanken, and et al. Protein microgels from amyloid fibril networks ACS Nano 9 2015 43 51
185. G. Castillo, K. Spinella, A. Poturnayova, M. Snejdarkova, L. Mosiello, and T. Hianik Detection of aflatoxin B-1 by aptamer-based biosensor using PAMAM dendrimers as immobilization platform Food Control 52 2015 9 18
186. S. Choi, and J.K. Park Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array Lab Chip 5 2005 1161 1167
187. L. Zou, C.S. Wu, Q. Wang, J. Zhou, K.Q. Su, H.B. Li, and et al. An improved sensitive assay for the detection of PSP toxins with neuroblastoma cell-based impedance biosensor Biosens. Bioelectron. 67 2015 458 464
188. G. Catanante, L. Espin, and J.L. Marty Sensitive biosensor based on recombinant PP1 alpha for microcystin detection Biosens. Bioelectron. 67 2015 700 707
189. J. Cecchetto, F.C. Carvalho, A. Santos, F.C.B. Fernandes, and P.R. Bueno An impedimetric biosensor to test neat serum for dengue diagnosis Sens. Actuators, B: Chem. 213 2015 150 154
190. X.Y. Ding, P. Li, S.C.S. Lin, Z.S. Stratton, N. Nama, F. Guo, and et al. Surface acoustic wave microfluidics Lab Chip 13 2013 3626 3649
191. X.L. Wang, S.X. Chen, M. Kong, Z.K. Wang, K.D. Costa, R.A. Li, and et al. Enhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologies Lab Chip 11 2011 3656 3662
192. Q. Hamid, C. Wang, J. Snyder, S. Williams, Y. Liu, and W. Sun Maskless fabrication of cell-laden microfluidic chips with localized surface functionalization for the co-culture of cancer cells Biofabrication 7 2015 015012
193. A.I. Shallan, P. Smejkal, M. Corban, R.M. Guijt, and M.C. Breadmore Cost-effective three-dimensional printing of visibly transparent microchips within minutes Anal. Chem. 86 2014 3124 3130
194. J.L. Erkal, A. Selimovic, B.C. Gross, S.Y. Lockwood, E.L. Walton, S. McNamara, and et al. 3D printed microfluidic devices with integrated versatile and reusable electrodes Lab Chip 14 2014 2023 2032
195. Y. Zhang, Y. Yu, and I.T. Ozbolat Direct bioprinting of vessel-like tubular microfluidic channels J. Nanotechnol. Eng. Med. 4 2013 0210011 210017
196. J.E. Snyder, Q. Hamid, C. Wang, R. Chang, K. Emami, H. Wu, and et al. Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip Biofabrication 3 2011
197. H.C. Shum, A. Bandyopadhyay, S. Bose, and D.A. Weitz Double emulsion droplets as microreactors for synthesis of mesoporous hydroxyapatite Chem. Mater. 21 2009 5548 5555
198. Y. Kitagawa, Y. Naganuma, Y. Yajima, M. Yamada, and M. Seki Patterned hydrogel microfibers prepared using multilayered microfluidic devices for guiding network formation of neural cells Biofabrication 6 2014
199. F. Stumpf, J. Schoendube, A. Gross, C. Rath, S. Niekrawietz, P. Koltay, and et al. Single-cell PCR of genomic DNA enabled by automated single-cell printing for cell isolation Biosens. Bioelectron. 69 2015 301 306
200. J. Schoendube, A. Yusof, K. Kalkandjiev, R. Zengerle, and P. Koltay Wafer level fabrication of single cell dispenser chips with integrated electrodes for particle detection J. Micromech. Microeng. 2015 25
201. A. Gross, J. Schöndube, S. Niekrawitz, W. Streule, L. Riegger, R. Zengerle, and et al. Single-cell printer: automated, on demand, and label free J. Lab. Autom. 18 2013 504 518
202. T. Kawahara, S. Ohashi, M. Hagiwara, Y. Yamanishi, and F. Arai Air-flow-based single-cell dispensing system Adv. Rob. 26 2012 291 306
203. L. Feng, Y. Sun, C. Ohsumi, and F. Arai Accurate dispensing system for single oocytes using air ejection Biomicrofluidics 7 2013 054113
204. A. van Reenen, A.M. de Jong, J.M.J. den Toonder, and M.W.J. Prins Integrated lab-on-chip biosensing systems based on magnetic particle actuation - a comprehensive review Lab Chip 14 2014 1966 1986
205. O. Strohmeier, S. Keil, B. Kanat, P. Patel, M. Niedrig, M. Weidmann, and et al. Automated nucleic acid extraction from whole blood, B. subtilis, E. coli and Rift Valley fever virus on a centrifugal microfluidic LabDisk RSC Adv. 5 2015 32144 32150
206. D. Jens, H. Stefan, L. Sascha, P. Sarah, S. Felix von, and Z. Roland The centrifugal microfluidic Bio-Disk platform J. Micromech. Microeng. 17 2007 S103
207. R. Gorkin, J. Park, J. Siegrist, M. Amasia, B.S. Lee, J.-M. Park, and et al. Centrifugal microfluidics for biomedical applications Lab Chip 10 2010 1758 1773
208. M. Keller, J. Naue, R. Zengerle, F. von Stetten, and U. Schmidt Automated forensic animal family identification by nested pcr and melt curve analysis on an off-the-shelf thermocycler augmented with a centrifugal microfluidic disk segment PLoS One 10 2015 e0131845
209. M.A. Witek, M.L. Hupert, D.S.W. Park, K. Fears, M.C. Murphy, and S.A. Soper 96-Well polycarbonate-based microfluidic titer plate for high-throughput purification of DNA and RNA Anal. Chem. 80 2008 3483 3491
210. P.J. Lee, N. Ghorashian, T.A. Gaige, and P.J. Hung Microfluidic system for automated cell-based assays J. Assoc. Lab. Autom. 12 2007 363 367