A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

Biomaterials

Volumn 34, Issue 28, 2013, Pages 6607-6614

  Yang, Kisuk ^a   Han, Sewoon ^b   Shin, Yoojin ^b   Ko, Eunkyung ^a   Kim, Jin ^a   Park, Kook In ^c   Chung, Seok ^b   Cho, Seung Woo ^a  

^a Yonsei University   (South Korea)

^b Korea University   (South Korea)

^c Yonsei University College of Medicine   (South Korea)

Author keywords

Differentiation; Hypoxia; Microfluidic array; Neural stem cells; Self renewal

Indexed keywords

EXTRACELLULAR MATRICES; HYPOXIA; LOW OXYGEN TENSIONS; MICROENVIRONMENTS; MICROFLUIDIC ARRAY; NEURAL STEM CELL; QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION; SELF-RENEWAL;

BIOMIMETICS; CELL CULTURE; DIFFERENTIATION (CALCULUS); ENZYME ACTIVITY; FUNCTIONAL POLYMERS; POLYMERASE CHAIN REACTION; STEM CELLS; THREE DIMENSIONAL; THREE DIMENSIONAL COMPUTER GRAPHICS;

MICROFLUIDICS;

ARTICLE; CELL HYPOXIA; CELL RENEWAL; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FETUS; HUMAN; IMMUNOCYTOCHEMISTRY; MICROFLUIDIC ANALYSIS; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME ULTRASOUND SCANNER;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL HYPOXIA; CELLS, CULTURED; HUMANS; MICROFLUIDICS; MODELS, BIOLOGICAL; NEURAL STEM CELLS;

EID: 84879461661     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2013.05.067     Document Type: Article

References (40)

1. Martino G., Pluchino S. The therapeutic potential of neural stem cells. Nat Rev Neurosci 2006, 7:395-406.
2. Johansson C.B., Momma S., Clarke D.L., Risling M., Lendahl U., Frisen J. Identification of a neural stem cell in the adult mammalian central nervous system. Cell 1999, 96:25-34.
3. Cummings B.J., Uchida N., Tamaki S.J., Salazar D.L., Hooshmand M., Summers R., et al. Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. Proc Natl Acad Sci U S A 2005, 102:14069-14074.
4. Lindvall O., Kokaia Z., Martinez-Serrano A. Stem cell therapy for human neurodegenerative disorders-how to make it work. Nat Med 2004, S42-S50. Suppl. 10.
5. Chojnacki A., Weiss S. Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells. Nat Protoc 2008, 3:935-940.
6. Lindvall O., Kokaia Z. Stem cells in human neurodegenerative disorders-time for clinical translation?. JClin Invest 2010, 120:29-40.
7. Pluchino S., Quattrini A., Brambilla E., Gritti A., Salani G., Dina G., et al. Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis. Nature 2003, 422:688-694.
8. Uchida N., Chen K., Dohse M., Hansen K.D., Dean J., Buser J.R., et al. Human neural stem cells induce functional myelination in mice with severe dysmyelination. Sci Transl Med 2012, 4:155ra36.
9. Gupta N., Henry R.G., Strober J., Kang S.M., Lim D.A., Bucci M., et al. Neural stem cell engraftment and myelination in the human brain. Sci Transl Med 2012, 4:155ra37.
10. Shen Q., Wang Y., Kokovay E., Lin G., Chuang S.M., Goderie S.K., et al. Adult SVZ stem cells lie in a vascular niche: a quantitative analysis of niche cell-cell interactions. Cell Stem Cell 2008, 3:289-300.
11. Kerever A., Schnack J., Vellinga D., Ichikawa N., Moon C., Arikawa-Hirasawa E., et al. Novel extracellular matrix structures in the neural stem cell niche capture the neurogenic factor fibroblast growth factor 2 from the extracellular milieu. Stem Cells 2007, 25:2146-2157.
12. Lee C., Hu J., Ralls S., Kitamura T., Loh Y.P., Yang Y., et al. The molecular profiles of neural stem cell niche in the adult subventricular zone. PLoS One 2012, 7:e50501.
13. Shen Q., Goderie S.K., Jin L., Karanth N., Sun Y., Abramova N., et al. Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells. Science 2004, 304:1338-1340.
14. Tavazoie M., Van der Veken L., Silva-Vargas V., Louissaint M., Colonna L., Zaidi B., et al. Aspecialized vascular niche for adult neural stem cells. Cell Stem Cell 2008, 3:279-288.
15. Kazanis I., ffrench-Constant C. Extracellular matrix and the neural stem cell niche. Dev Neurobiol 2011, 71:1006-1017.
16. Fuentealba L.C., Obernier K., Alvarez-Buylla A. Adult neural stem cells bridge their niche. Cell Stem Cell 2012, 10:698-708.
17. Alvarez-Buylla A., Lim D.A. For the long run: maintaining germinal niches in the adult brain. Neuron 2004, 41:683-686.
18. Mohyeldin A., Garzon-Muvdi T., Quinones-Hinojosa A. Oxygen in stem cell biology: a critical component of the stem cell niche. Cell Stem Cell 2010, 7:150-161.
19. Storch A., Paul G., Csete M., Boehm B.O., Carvey P.M., Kupsch A., et al. Long-term proliferation and dopaminergic differentiation of human mesencephalic neural precursor cells. Exp Neurol 2001, 170:317-325.
20. Studer L., Csete M., Lee S.H., Kabbani N., Walikonis J., Wold B., et al. Enhanced proliferation, survival, and dopaminergic differentiation of CNS precursors in lowered oxygen. JNeurosci 2000, 20:7377-7383.
21. Shin Y., Han S., Jeon J.S., Yamamoto K., Zervantonakis I.K., Sudo R., et al. Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels. Nat Protoc 2012, 7:1247-1259.
22. Taylor A.M., Jeon N.L. Micro-scale and microfluidic devices for neurobiology. Curr Opin Neurobiol 2010, 20:640-647.
23. Funamoto K., Zervantonakis I.K., Liu Y., Ochs C.J., Kim C., Kamm R.D. Anovel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment. Lab Chip 2012, 12:4855-4863.
24. Yang F., Cho S.W., Son S.M., Hudson S.P., Bogatyrev S., Keung L., et al. Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D. Biomacromolecules 2010, 11:1909-1914.
25. Vickerman V., Blundo J., Chung S., Kamm R. Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging. Lab Chip 2008, 8:1468-1477.
26. Huang C.P., Lu J., Seon H., Lee A.P., Flanagan L.A., Kim H.Y., et al. Engineering microscale cellular niches for three-dimensional multicellular co-cultures. Lab Chip 2009, 9:1740-1748.
27. Han S., Yang K., Shin Y., Lee J.S., Kamm R.D., Chung S., et al. Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device. Lab Chip 2012, 12:2305-2308.
28. Yang K., Lee J.S., Kim J., Lee Y.B., Shin H., Um S.H., et al. Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering. Biomaterials 2012, 33:6952-6964.
29. Park H.J., Lee J., Kim M.J., Kang T.J., Jeong Y., Um S.H., et al. Sonic hedgehog intradermal gene therapy using a biodegradable poly(beta-amino esters) nanoparticle to enhance wound healing. Biomaterials 2012, 33:9148-9156.
30. Bhattacharya S., Datta A., Berg J.M., Gangopadhyay S. Studies on surface wettability of poly(dimethyl) siloxane (PDMS) and glass under oxygen-plasma treatment and correlation with bond strength. JMicroelectromech Syst 2005, 14:590-597.
31. Carmeliet P., Dor Y., Herbert J.M., Fukumura D., Brusselmans K., Dewerchin M., et al. Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature 1998, 394:485-490.
32. 2 sensing. Science 2001, 292:464-468.
33. Semenza G.L. Hydroxylation of HIF-1: oxygen sensing at the molecular level. Physiology (Bethesda) 2004, 19:176-182.
34. Szablowska-Gadomska I., Zayat V., Buzanska L. Influence of low oxygen tensions on expression of pluripotency genes in stem cells. Acta Neurobiol Exp (Wars) 2011, 71:86-93.
35. Forristal C.E., Wright K.L., Hanley N.A., Oreffo R.O., Houghton F.D. Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions. Reproduction 2010, 139:85-97.
36. Gustafsson M.V., Zheng X., Pereira T., Gradin K., Jin S., Lundkvist J., et al. Hypoxia requires notch signaling to maintain the undifferentiated cell state. Dev Cell 2005, 9:617-628.
37. Mathieu J., Zhang Z., Zhou W., Wang A.J., Heddleston J.M., Pinna C.M., et al. HIF induces human embryonic stem cell markers in cancer cells. Cancer Res 2011, 71:4640-4652.
38. Wojcik-Stanaszek L., Gregor A., Zalewska T. Regulation of neurogenesis by extracellular matrix and integrins. Acta Neurobiol Exp (Wars) 2011, 71:103-112.
39. Santilli G., Lamorte G., Carlessi L., Ferrari D., Rota Nodari L., Binda E., et al. Mild hypoxia enhances proliferation and multipotency of human neural stem cells. PLoS One 2010, 5:e8575.
40. Giese A.K., Frahm J., Hubner R., Luo J., Wree A., Frech M.J., et al. Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells. BMC Cell Biol 2010, 11:94.