Cucurbitacin B induces DNA damage and autophagy mediated by reactive oxygen species (ROS) in MCF-7 breast cancer cells

Journal of Natural Medicines

Volumn 69, Issue 4, 2015, Pages 522-530

  Ren, Guowen ^a   Sha, Tongye ^b   Guo, Jiajie ^a   Li, Wenxue ^a   Lu, Jinjian ^a   Chen, Xiuping ^a  

^a University of Macau   (Macao)

^b Rizhao Hospital of TCM   (China)

Author keywords

Autophagy; Breast cancer; Cucurbitacin B; DNA damage; ROS

Indexed keywords

ACETYLCYSTEINE; ANTINEOPLASTIC AGENT; ATM PROTEIN; ATR PROTEIN; CUCURBITACIN B; DANSYLCADAVERINE; GAMMA HISTONE H2AX; HISTONE H2AX; NATURAL PRODUCT; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG; BIOLOGICAL PRODUCT; TRITERPENE;

ANTINEOPLASTIC ACTIVITY; ARTICLE; AUTOPHAGY; BREAST CANCER; CELL PROLIFERATION; CELL VIABILITY; COLONY FORMATION; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; ENZYME PHOSPHORYLATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MCF 7 CELL LINE; MTT ASSAY; PROTEIN EXPRESSION; WESTERN BLOTTING; BREAST TUMOR; CHEMISTRY; DRUG EFFECTS; FEMALE; METABOLISM; PHOSPHORYLATION;

AUTOPHAGY; BIOLOGICAL PRODUCTS; BREAST NEOPLASMS; DNA DAMAGE; FEMALE; HUMANS; MCF-7 CELLS; PHOSPHORYLATION; REACTIVE OXYGEN SPECIES; TRITERPENES;

EID: 84941259027     PISSN: 13403443     EISSN: 18610293     Source Type: Journal    
DOI: 10.1007/s11418-015-0918-4     Document Type: Article

References (46)

1. Chen JC, Chiu MH, Nie RL, Cordell GA, Qiu SX (2005) Cucurbitacins and cucurbitane glycosides: structures and biological activities. Nat Prod Rep 22:386-399
2. Chen X, Bao J, Guo J, Ding Q, Lu J, Huang M, Wang Y (2012) Biological activities and potential molecular targets of cucurbitacins: a focus on cancer. Anticancer Drugs 23:777-787
3. Liu T, Zhang M, Zhang H, Sun C, Deng Y (2008) Inhibitory effects of cucurbitacin B on laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 265:1225-1232
4. Thoennissen NH, Iwanski GB, Doan NB, Okamoto R, Lin P, Abbassi S, Song JH, Yin D, Toh M, Xie WD (2009) Cucurbitacin B induces apoptosis by inhibition of the JAK/STAT pathway and potentiates antiproliferative effects of gemcitabine on pancreatic cancer cells. Cancer Res 69:5876-5884
5. Zhang M, Zhang H, Sun C, Shan X, Yang X, Li-Ling J, Deng Y (2009) Targeted constitutive activation of signal transducer and activator of transcription 3 in human hepatocellular carcinoma cells by cucurbitacin B. Cancer Chemother Pharmacol 63:635-642
6. Chan KT, Meng FY, Li Q, Ho CY, Lam TS, To Y, Lee WH, Li M, Chu KH, Toh M (2010) Cucurbitacin B induces apoptosis and S phase cell cycle arrest in BEL-7402 human hepatocellular carcinoma cells and is effective via oral administration. Cancer Lett 294:118-124
7. Zhang Y, Ouyang D, Xu L, Ji Y, Zha Q, Cai J, He X (2011) Cucurbitacin B induces rapid depletion of the G-actin pool through reactive oxygen species-dependent actin aggregation in melanoma cells. Acta Biochim Biophys Sin (Shanghai) 43:556-567
8. Kausar H, Munagala R, Bansal SS, Aqil F, Vadhanam MV, Gupta RC (2013) Cucurbitacin B potently suppresses non-small-cell lung cancer growth: identification of intracellular thiols as critical targets. Cancer Lett 332:35-45
9. Liu T, Peng H, Zhang M, Deng Y, Wu Z (2010) Cucurbitacin B, a small molecule inhibitor of the Stat3 signaling pathway, enhances the chemosensitivity of laryngeal squamous cell carcinoma cells to cisplatin. Eur J Pharmacol 641:15-22
10. Iwanski GB, Lee DH, En-Gal S, Doan NB, Castor B, Vogt M, Toh M, Bokemeyer C, Said JW, Thoennissen NH (2010) Cucurbitacin B, a novel in vivo potentiator of gemcitabine with low toxicity in the treatment of pancreatic cancer. Br J Pharmacol 160:998-1007
11. Lee DH, Thoennissen NH, Goff C, Iwanski GB, Forscher C, Doan NB, Said JW, Phillip Koeffler H (2011) Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma. Cancer Lett 306:161-170
12. Aribi A, Gery S, Lee DH, Thoennissen NH, Thoennissen GB, Alvarez R, Ho Q, Lee K, Doan NB, Chan KT (2013) The triterpenoid cucurbitacin B augments the antiproliferative activity of chemotherapy in human breast cancer. Int J Cancer 132:2730-2737
13. Wakimoto N, Yin D, O'Kelly J, Haritunians T, Karlan B, Said J, Xing H, Koeffler HP (2008) Cucurbitacin B has a potent antiproliferative effect on breast cancer cells in vitro and in vivo. Cancer Sci 99:1793-1797
14. Gupta P, Srivastava SK (2014) Inhibition of Integrin-HER2 signaling by Cucurbitacin B leads to in vitro and in vivo breast tumor growth suppression. Oncotarget 5:1812-1828
15. Duangmano S, Sae-Lim P, Suksamrarn A, Domann FE, Patmasiriwat P (2012) Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation. BMC Complement Altern Med 12:185
16. Duangmano S, Dakeng S, Jiratchariyakul W, Suksamrarn A, Smith DR, Patmasiriwat P (2010) Antiproliferative effects of cucurbitacin B in breast cancer cells: down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle. Int J Mol Sci 11:5323-5338
17. Wakimoto N, Yin D, O'Kelly J, Haritunians T, Karlan B, Said J, Xing H, Koeffler HP (2008) Cucurbitacin B has a potent antiproliferative effect on breast cancer cells in vitro and in vivo. Cancer Sci 99:1793-1797
18. Dakeng S, Duangmano S, Jiratchariyakul W, Up Y, Bogler O, Patmasiriwat P (2012) Inhibition of Wnt signaling by cucurbitacin B in breast cancer cells: reduction of Wnt-associated proteins and reduced translocation of galectin-3-mediated beta-catenin to the nucleus. J Cell Biochem 113:49-60
19. Ray PD, Huang B-W, Tsuji Y (2012) Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 24:981-990
20. Zhang T, Li Y, Park KA, Byun HS, Won M, Jeon J, Lee Y, Seok JH, Choi S-W, Lee S-H (2012) Cucurbitacin induces autophagy through mitochondrial ROS production which counteracts to limit caspase-dependent apoptosis. Autophagy 8:559-576
21. Yasuda S, Yogosawa S, Izutani Y, Nakamura Y, Watanabe H, Sakai T (2010) Cucurbitacin B induces G2 arrest and apoptosis via a reactive oxygen species-dependent mechanism in human colon adenocarcinoma SW480 cells. Mol Nutr Food Res 54:559-565
22. Filomeni G, De Zio D, Cecconi F (2014) Oxidative stress and autophagy: the clash between damage and metabolic needs. Cell Death Differ 22:377-388
23. Guo J, Wu G, Bao J, Hao W, Lu J, Chen X (2014) Cucurbitacin B induced ATM-mediated DNA damage causes G2/M cell cycle arrest in a ROS-dependent manner. PLoS One 9:e88140
24. Guo J, Zhao W, Hao W, Ren G, Lu J, Chen X (2014) Cucurbitacin B induces DNA damage, G2/M phase arrest, and apoptosis mediated by reactive oxygen species (ROS) in leukemia K562 cells. Anticancer Agents Med Chem 14:1146-1153
25. Munafo DB, Colombo MI (2001) A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation. J Cell Sci 114:3619-3629
26. Lord CJ, Ashworth A (2012) The DNA damage response and cancer therapy. Nature 481:287-294
27. Garcia-Canton C, Anadon A, Meredith C (2012) gammaH2AX as a novel endpoint to detect DNA damage: applications for the assessment of the in vitro genotoxicity of cigarette smoke. Toxicol In Vitro 26:1075-1086
28. Mah LJ, El-Osta A, Karagiannis TC (2010) gammaH2AX: a sensitive molecular marker of DNA damage and repair. Leukemia 24:679-686
29. Maréchal A, Zou L (2013) DNA damage sensing by the ATM and ATR kinases. Cold Spring Harb Perspect Biol 5(9)
30. Smith J, Tho LM, Xu N, Gillespie DA (2010) The ATM-Chk2 and ATR-Chk1 pathways in DNA damage signaling and cancer. Adv Cancer Res 108:73-112
31. Nagelkerke A, Bussink J, Geurts-Moespot A, Sweep FC, Span PN (2014) Therapeutic targeting of autophagy in cancer. Part II: pharmacological modulation of treatment-induced autophagy. Semin Cancer Biol 31:99-105
32. Thorburn A, Thamm DH, Gustafson DL (2014) Autophagy and cancer therapy. Mol Pharmacol 85:830-838
33. Zhang T, Li Y, Park KA, Byun HS, Won M, Jeon J, Lee Y, Seok JH, Choi SW, Lee SH, Man Kim J, Lee JH, Son CG, Lee ZW, Shen HM, Hur GM (2012) Cucurbitacin induces autophagy through mitochondrial ROS production which counteracts to limit caspase-dependent apoptosis. Autophagy 8:559-576
34. He J, Wang Y, Xu LH, Qiao J, Ouyang DY, He XH (2013) Cucurbitacin IIa induces caspase-3-dependent apoptosis and enhances autophagy in lipopolysaccharide-stimulated RAW 264.7 macrophages. Int Immunopharmacol 16:27-34
35. Zhu JS, Ouyang DY, Shi ZJ, Xu LH, Zhang YT, He XH (2012) Cucurbitacin B induces cell cycle arrest, apoptosis and autophagy associated with G actin reduction and persistent activation of cofilin in Jurkat cells. Pharmacology 89:348-356
36. Yuan G, Yan SF, Xue H, Zhang P, Sun JT, Li G (2014) Cucurbitacin I induces protective autophagy in glioblastoma in vitro and in vivo. J Biol Chem 289:10607-10619
37. Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y, Yoshimori T (2000) LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J 19:5720-5728
38. Mizushima N, Yoshimori T, Levine B (2010) Methods in mammalian autophagy research. Cell 140:313-326
39. Vázquez CL, Colombo MI. (2009) Chapter 6 assays to assess autophagy induction and fusion of autophagic vacuoles with a degradative compartment, using monodansylcadaverine (MDC) and DQ'BSA. In: Daniel JK (ed) Methods in enzymology. Academic Press, pp 85-95
40. Rubinsztein DC, Codogno P, Levine B (2012) Autophagy modulation as a potential therapeutic target for diverse diseases. Nat Rev Drug Discov 11:709-730
41. Kang R, Zeh HJ, Lotze MT, Tang D (2011) The Beclin 1 network regulates autophagy and apoptosis. Cell Death Differ 18:571-580
42. Myeku N, Figueiredo-Pereira ME (2011) Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem 286:22426-22440
43. Scherz-Shouval R, Elazar Z (2007) ROS, mitochondria and the regulation of autophagy. Trends Cell Biol 17:422-427
44. Azad MB, Chen Y, Gibson SB (2009) Regulation of autophagy by reactive oxygen species (ROS): implications for cancer progression and treatment. Antioxid Redox Signal 11:777-790
45. Kurz EU, Lees-Miller SP (2004) DNA damage-induced activation of ATM and ATM-dependent signaling pathways. DNA Repair 3:889-900
46. Yasuda S, Yogosawa S, Izutani Y, Nakamura Y, Watanabe H, Sakai T (2010) Cucurbitacin B induces G2 arrest and apoptosis via a reactive oxygen species-dependent mechanism in human colon adenocarcinoma SW480 cells. Mol Nutr Food Res 54:559-565