MegaTevs: Single-chain dual nucleases for efficient gene disruption

Nucleic Acids Research

Volumn 42, Issue 13, 2014, Pages 8816-8829

  Wolfs, Jason M ^a   Dasilva, Matthew ^a   Meister, Sarah E ^a   Wang, Xu ^b   Schild Poulter, Caroline ^a,b   Edgell, David R ^a  

^a WESTERN UNIVERSITY   (Canada)

^b ROBARTS RESEARCH INSTITUTE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINYLASPARAGINYLASPARAGINYLASPARAGINYLGLYCINE; DOUBLE STRANDED DNA; HYBRID PROTEIN; MEGANUCLEASE TEV FUSION PROTEIN; NUCLEASE; POLYPEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DNA STRAND BREAKAGE; ENZYME ACTIVE SITE; ENZYME ENGINEERING; GENE DELETION; GENE DISRUPTION; GENE FREQUENCY; GENE TARGETING; HEK293 CELL LINE; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN CLEAVAGE; SEQUENCE ANALYSIS;

CATALYTIC DOMAIN; DNA BREAKS, DOUBLE-STRANDED; DNA CLEAVAGE; DNA REPAIR; ENDODEOXYRIBONUCLEASES; GENETIC ENGINEERING; HEK293 CELLS; HUMANS; MUTAGENESIS; NUCLEOTIDE MOTIFS; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION;

EID: 84905586329     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gku573     Document Type: Article

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