Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 11, 2014, Pages 4061-4066

  Takeuchi, Ryo ^a   Choi, Michael ^a   Stoddard, Barry L ^a  

^a Fred Hutchinson Cancer Research Center   (United States)

Author keywords

Gene modification; Gene therapy; Protein engineering

Indexed keywords

ENDONUCLEASE; GENOMIC DNA; NUCLEOTIDE; PROTEIN VARIANT;

ARTICLE; CELL COMPARTMENTALIZATION; DNA BINDING; DNA FLANKING REGION; DNA MODIFICATION; EXCISION; EXON; GENE THERAPY; HUMAN; HUMAN CELL; IN VITRO STUDY; INTRON; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION;

GENE MODIFICATION; GENE THERAPY; PROTEIN ENGINEERING;

BASE SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA; DNA CLEAVAGE; ENDODEOXYRIBONUCLEASES; GENETIC THERAPY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

EID: 84896504411     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1321030111     Document Type: Article

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