I-Ppol and I-CreI homing site sequence degeneracy determined by random mutagenesis and sequential in vitro enrichment

Journal of Molecular Biology

Volumn 280, Issue 3, 1998, Pages 345-353

  Argast, Gretchen M ^a   Stephens, Kathryn M ^a   Emond, Mary J ^a   Monnat Jr , Raymond J ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Group I intron; Homing endonuclease; In vitro enrichment; Intron homing; Sequence degeneracy

Indexed keywords

DODECYL SULFATE SODIUM; ENDONUCLEASE; DEOXYRIBONUCLEASE; ENDODEOXYRIBONUCLEASE CREI; I PPO ENDONUCLEASE; I-PPO ENDONUCLEASE; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA DENATURATION; ESCHERICHIA COLI; EUKARYOTE; MOLECULAR RECOGNITION; MUTAGENESIS; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN NUCLEIC ACID INTERACTION; SEQUENCE HOMOLOGY; BINDING SITE; ENZYME SPECIFICITY; GENETICS; INTRON; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; SEQUENCE ALIGNMENT;

ESCHERICHIA COLI;

BASE SEQUENCE; BINDING SITES; DNA RESTRICTION ENZYMES; ENDODEOXYRIBONUCLEASES; ESCHERICHIA COLI; INTRONS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PLASMIDS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

EID: 0032540751     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.1886     Document Type: Article

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